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Invitrogen
VDAC Polyclonal Antibody
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FIGURE: 1 / 51
VDAC Antibody (PA1-954A) in ICC/IF
Immunofluorescence analysis of VDAC was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VDAC Polyclonal Antibody (Product # PA1-954A) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
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.jpg?time=20220826?time=20220826 "VDAC Antibody (PA1-954A) in WB")
.jpg?time=20220826?time=20220826 "VDAC Antibody (PA1-954A) in WB")
Product Details
PA1-954A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Bovine,
C. elegans,
Hamster,
Human,
Mouse,
Rat,
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C N(185) D G T E F G G S I Y Q K(197)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-954A detects voltage-dependent anion channel (VDAC) from human, non-human primates, mouse, and rat samples.
PA1-954A has been successfully used in IF/ICC, IHC (P) and Western blot procedures. By Western blot, this antibody detects a single, prominent ~31 kDa band representing VDAC from rat heart extract.
PA1-954A immunizing peptide corresponds to the C-terminal amino acid residues 185-197 of human VDAC3. This sequence is completely conserved between isoforms 1, 2 and 3 of human VDAC and PA1-954A detects endogenous levels of total VDAC. PA1-954A immunizing peptide (Cat. # PEP-082) is available for use in neutralization and control experiments.
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC constitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hVDAC1; MGC111064; Mitochondrial Porin; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN-31-HL; rVDAC1; VD; VDAC-1; VDAC-5; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5
Gene Aliases: AL033343; PORIN; VDAC; VDAC-1; VDAC1; Vdac5
UniProt ID: (Human) P21796, (Mouse) Q60932, (Rat) Q9Z2L0
Entrez Gene ID: (Human) 7416, (Mouse) 22333, (Rat) 83529
behavioral fear response
anion transport
mitochondrial calcium ion transport
apoptotic process
neuron-neuron synaptic transmission
learning
viral process
macroautophagy
epithelial cell differentiation
anion transmembrane transport
regulation of mitophagy
regulation of anion transmembrane transport
negative regulation of reactive oxygen species metabolic process
transport
ion transport
chemical synaptic transmission
regulation of anion transport
transmembrane transport
ion transmembrane transport
regulation of ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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