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Invitrogen
VGLUT1 Polyclonal Antibody
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VGLUT1 Antibody (48-2400) in ICC/IF
Immunofluorescence analysis of VGLUT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VGLUT1 Rabbit Polyclonal Antibody (Product # 48-2400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
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.jpg?time=20240507?time=20240507 "VGLUT1 Antibody (48-2400) in ICC/IF")
产品信息
48-2400
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q3TXX4), which is 93% homologous to human and rat.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for the VGLUT1 protein. On western blots, it identifies the target band at ~55 kDa. Reactivity has been confirmed with mouse brain homogenates by western blotting, with mouse hippocampus frozen sections by immunohistochemistry, and in immunofluorescence analysis with human glutamate neurons derived from iPSCs.
靶标信息
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; solute carrier family 17 (vesicular glutamate transporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1
基因别名: 2900052E22Rik; AI851913; BNPI; SLC17A7; VGLUT1
UniProt ID: (Human) Q9P2U7, (Rat) Q62634, (Mouse) Q3TXX4
Entrez Gene ID: (Human) 57030, (Rat) 116638, (Mouse) 72961
L-glutamate transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
extracellular-glutamate-gated chloride channel activity
sodium:inorganic phosphate symporter activity
sodium-dependent phosphate transmembrane transporter activity
symporter activity
secondary carrier transporter
neural retina development
ion transport
phosphate ion transport
neurotransmitter transport
brain development
long-term memory
glutamate secretion
synaptic transmission, glutamatergic
phosphate ion transmembrane transport
sodium ion transmembrane transport
sequestering of neurotransmitter
sodium-dependent phosphate transport
L-glutamate import
excitatory postsynaptic potential
L-glutamate transmembrane transport
synaptic vesicle lumen acidification
chloride transmembrane transport
chemical synaptic transmission
transport
sodium ion transport
response to stimulus
transmembrane transport
