Invitrogen

VPS34 Polyclonal Antibody

Name: VPS34 Polyclonal Antibody
Brand: Invitrogen
SKU: 38-2100
Availability: InStock

高级验证

27个已发表图片

39 篇参考文献

查看其他20个VPS34抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

VPS34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 33

VPS34 Antibody (38-2100) in IHC (P)

Immunohistochemistry analysis of VPS34 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temper... View More

VPS34 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

VPS34 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

VPS34 Antibody in Immunohistochemistry (IHC)

VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)

VPS34 Antibody in Immunoprecipitation (IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

VPS34 Antibody — Fig. 6. Vps34 participates in NS4B- and HCV-induced autophagy. (A and B) GFP-LC3 cells were transfected with HA-NS4B (A) or infected with HCV JC1 virus (B). After 5 h, cells were treated with 3-MA at various concentrations for an additional 40 h of incubation and then fixed for indirect immunofluorescence staining with anti-HA or anticore antibody. GFP-LC3 (green) and HA-tagged NS4B or HCV core (red) are shown. (C and D) Huh7.5 cells were transduced with lentiviruses expressing shRNA against Vps34 (two different clones denoted as shVps34#1 [lanes 2 and 5] and shVps34#2 [lanes 3 and 6]) or shLacZ (as control [lanes 1 and 4]) and then treated with puromycin for selection of shRNA-carrying cells. At 4 days posttransduction, 1 x 10 5 of the transduced cells were reseeded into a 12-well plate for further experiments. The knockdown cells were transfected with 0.5 mug NS4B for 40 h (C) or infected with HCV JC1 virus at an MOI of 0.1 for 72 h (D). The cells were harvested, and the lysates were used for Western blot analysis with the indicated antibodies. The relative densitometric LC3-II/actin ratios are shown below the blots. Mock vector-transfected and noninfected cells were used as a control. {KD}

产品信息

38-2100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看24篇已发表文章 24篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看6篇已发表文章 6篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Hamster, Human, Mouse, Non-human primate, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the mid region of the human VPS34 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533360

靶标信息

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphoinositide-3-kinase class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; ptdIns-3-kinase type 3

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基因别名: 5330434F23Rik; Pik3c3; Vps34

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UniProt ID: (Mouse) Q6PF93

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Entrez Gene ID: (Mouse) 225326

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功能