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VPS53 Polyclonal Antibody
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FIGURE: 1 / 3
VPS53 Antibody (600-401-FX3) in ICC/IF
Immunocytochemistry of VPS53 antibody. Tissue: 293 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: VPS53 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: VPS53 is nuclear and occasionally cytoplasmic. Staining: VPS53 as a precipitated brown signal with hematoxylin purple counterstain.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-FX3
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-VPS53 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the C-terminus of human VPS53.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hepatocellular carcinoma suppressor 1; hVps53L; unnamed protein product; vacuolar protein sorting 53 homolog; VPS53 GARP complex subunit; VPS53, GARP complex subunit
Gene Aliases: 2010002A08Rik; 2310040I21Rik; 3100002B05Rik; HCCS1; hVps53L; PCH2E; pp13624; RGD1311391
UniProt ID: (Human) Q5VIR6, (Mouse) Q8CCB4
Entrez Gene ID: (Human) 55275, (Mouse) 68299, (Rat) 287535
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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