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Invitrogen
WDR45 Polyclonal Antibody
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FIGURE: 1 / 2
WDR45 Antibody (PA5-77801) in ICC/IF
Immunofluorescent analysis of WDR45B in human HeLa cells (human cervical cancer). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with WDR45B polyclonal antibody (Product # PA5-77801) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Merged image. Magnificat... View More
Product Details
PA5-77801
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the C-terminal of Human WIPI4 (aa 312-322)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of PA5-77801 was sufficient for detection of WIPI4 in 15 µg of Human HeLa Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 40 kDa.
Target Information
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: neurodegeneration with brain iron accumulation 4; neurodegeneration with brain iron accumulation 5; WD repeat domain phosphoinositide-interacting protein 4; WD repeat domain, X-linked 1; WD repeat-containing protein 45; WD45 repeat protein interacting with phosphoinositides 4; WIPI-4
Gene Aliases: JM5; NBIA4; NBIA5; WDR45; WDRX1; WDRXI4; WIPI-4; WIPI4
UniProt ID: (Human) Q6IBS7, (Rat) Q5U2Y0
Entrez Gene ID: (Human) 11152, (Rat) 302559
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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