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Invitrogen
XPB Polyclonal Antibody
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FIGURE: 1 / 3
XPB Antibody (PA5-104396) in IHC (P)
Immunohistochemistry analysis of XPB in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with XPB polyclonal antibody (Product # PA5-104396) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104396
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ERCC3(Accession P19447), corresponding to amino acid residues D33-H83.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ERCC3.
Target Information
The human XPB DNA helicase is a subunit of the DNA repair/basal transcription factor TFIIH, and is involved in early steps of the nucleotide excision repair (NER) pathway. Two distinct clinical phenotypes, xeroderma pigmentosum associated with Cockayne's syndrome (XP/CS) and trichothiodystrophy (TTD), can be due to mutations in the XPB gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2-p89; DNA 3'-5' helicase/translocase XPB; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; excision repair 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; General transcription and DNA repair factor IIH helicase/translocase subunit XPB; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH subunit XPB; Xeroderma pigmentosum group B-complementing protein
Gene Aliases: BTF2 p89; Ercc-3; ERCC3; XPB; XPBC
UniProt ID: (Mouse) P49135, (Rat) Q4G005
Entrez Gene ID: (Mouse) 13872, (Rat) 291703
nucleotide binding
DNA binding
ATP-dependent DNA helicase activity
helicase activity
protein kinase activity
ATP binding
GTP binding
protein C-terminus binding
DNA-dependent ATPase activity
RNA polymerase II carboxy-terminal domain kinase activity
hydrolase activity
ATPase activity
dATP binding
peptide binding
3'-5' DNA helicase activity
protein N-terminus binding
protein binding
transcription factor binding
DNA metabolism protein
DNA helicase
nucleic acid metabolism protein
nucleotide-excision repair, DNA duplex unwinding
DNA topological change
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein phosphorylation
apoptotic process
cellular response to DNA damage stimulus
response to oxidative stress
protein localization
response to UV
UV protection
nucleotide-excision repair, DNA incision
hair cell differentiation
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
regulation of mitotic cell cycle phase transition
response to hypoxia
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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