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Invitrogen
alpha Tubulin Monoclonal Antibody (YL1/2)
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alpha Tubulin Antibody (MA1-80017) in ICC/IF
Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin (YL1/2) Rat Monoclonal Antibody at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temper... View More
产品信息
MA1-80017
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Fruit fly,
Human,
Mouse,
Plant,
Pig,
Rabbit,
Rat,
Xenopus,
Yeast
已发表种属
Arthropod,
Bacteria,
C. elegans,
Cnidarian,
Dog,
Fruit fly,
Fungi,
Human,
Insect,
Mouse,
Non-human primate,
Plant,
Protozoa,
Rabbit,
Rat
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
YL1/2
抗原
Yeast tubulin.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody appears to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology. Does not react with nephrotoma suturalis.
This antibody is suitable for use as a loading control.The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
靶标信息
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: &agr-tubulin 84B; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; AT25469p; beta-tubulin T beta15 (aa 1-445); CG1913-PA; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog; Tubulin-alpha1; tubulin1alpha; tubulin84B
基因别名: 1t; 2410129E14Rik; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; Alpha-Tubulin84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; BEST:LD32507; brdp; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; PMGYSA; RGD1309427; RGD1565476; T; Talpha1; TBA1_DROME; Tub; Tub 84B; TUB1; Tub1C; Tub84B; Tuba-1; TUBA1; TUBA1A; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; tubal2; TUBAL3; Tubalpha1; Tubb2; Tubb2a; TUBB2B; tubulin; XELAEV_18019849mg; XELAEV_18020901mg; YML085C
UniProt ID: (Baker's yeast) P09733, (Human) Q71U36, (Pig) Q2XVP4, (Human) P68363, (Human) Q9BQE3, (Human) Q6PEY3, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) A8K068, (Mouse) P68369, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Fruit fly) P06603
Entrez Gene ID: (Dog) 106557476, (Baker's yeast) 854889, (Pig) 100135051, (Human) 7846, (Pig) 733594, (Human) 10376, (Rabbit) 100009327, (Human) 84790, (Human) 7278, (Human) 112714, (Pig) 100151951, (Dog) 478918, (Human) 7277, (Rabbit) 100354352, (Human) 79861, (Rabbit) 100353478, (Dog) 487148, (Pig) 100623355, (Dog) 478702, (Human) 347733, (Pig) 100153507, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081, (Fruit fly) 40848, (Xenopus) 431994, (Xenopus) 100036943
nucleotide binding
molecular_function
structural constituent of cytoskeleton
GTP binding
GTPase activity
structural molecule activity
protein binding
protein domain specific binding
double-stranded RNA binding
ubiquitin protein ligase binding
enzyme binding
protein heterodimerization activity
tubulin
mitotic sister chromatid segregation
microtubule cytoskeleton organization
mitotic cell cycle
nuclear division
microtubule-based process
nuclear migration along microtubule
homologous chromosome segregation
nuclear migration by microtubule mediated pushing forces
G2/M transition of mitotic cell cycle
cytoskeleton organization
cytoskeleton-dependent intracellular transport
cell division
cellular response to interleukin-4
biological_process
platelet degranulation
neuron migration
protein polymerization
metabolic process
lysosome localization
