Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
beta Arrestin 1 Antibody (PA5-35089) in ICC/IF
Immunocytochemistry analysis of beta Arrestin 1 in A549 cells. Samples were incubated in beta Arrestin 1 polyclonal antibody (Product # PA5-35089) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
产品信息
PA5-35089
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 336-363 amino acids from the C-terminal region of human ARRB1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2552399
产品详细信息
This antibody is predicted to react with bovine, mouse, rat and rabbit based on sequence homology.
靶标信息
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: arrestin; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1; Non-visual arrestin-2
基因别名: ARB1; ARR1; ARRB1
UniProt ID: (Human) P49407
Entrez Gene ID: (Human) 408
histone acetyltransferase activity
enzyme inhibitor activity
GTPase activator activity
insulin-like growth factor receptor binding
protein binding
transcription factor binding
estrogen receptor binding
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
alpha-1A adrenergic receptor binding
alpha-1B adrenergic receptor binding
angiotensin receptor binding
follicle-stimulating hormone receptor binding
V2 vasopressin receptor binding
AP-2 adaptor complex binding
clathrin adaptor activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
transcription regulatory region DNA binding
ion channel binding
protein phosphorylated amino acid binding
scaffold/adaptor protein
activation of MAPK activity
positive regulation of protein phosphorylation
G-protein coupled receptor internalization
positive regulation of receptor internalization
transcription from RNA polymerase II promoter
apoptotic process
phototransduction
protein transport
protein ubiquitination
platelet activation
negative regulation of protein ubiquitination
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
positive regulation of protein binding
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
positive regulation of peptidyl-serine phosphorylation
negative regulation of GTPase activity
positive regulation of smooth muscle cell apoptotic process
positive regulation of Rho protein signal transduction
positive regulation of histone acetylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
response to antidepressant
follicle-stimulating hormone signaling pathway
stress fiber assembly
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of histone H4 acetylation
