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Invitrogen
eNOS Polyclonal Antibody
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产品信息
PA1-037
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Human,
Mouse,
Pig,
Rat
已发表种属
Amphibian,
Bovine,
C. elegans,
Cat,
Dog,
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombinant protein. This antibody does not detect human iNOS or rat nNOS.
PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells.
The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
靶标信息
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183
基因别名: 2310065A03Rik; cNOS; EC-NOS; ECNOS; eNOS; NOS; Nos-3; NOS3; NOSIII
UniProt ID: (Pig) Q28969, (Human) P29474, (Mouse) P70313, (Rat) Q62600
Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846, (Dog) 403784, (Mouse) 18127, (Rat) 24600
nitric-oxide synthase activity
calmodulin binding
FMN binding
heme binding
metal ion binding
flavin adenine dinucleotide binding
NADP binding
actin monomer binding
iron ion binding
protein binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
scaffold protein binding
actin binding
NADPH-hemoprotein reductase activity
beta-catenin binding
oxidoreductase activity
cadherin binding
nitric-oxide synthase binding
Hsp90 protein binding
oxidoreductase
protein modifying enzyme
arginine catabolic process
nitric oxide biosynthetic process
mitochondrion organization
blood coagulation
positive regulation of guanylate cyclase activity
oxidation-reduction process
cellular response to laminar fluid shear stress
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of leukocyte cell-cell adhesion
positive regulation of blood vessel diameter
angiogenesis
ovulation from ovarian follicle
in utero embryonic development
blood vessel remodeling
regulation of sodium ion transport
regulation of the force of heart contraction by chemical signal
regulation of systemic arterial blood pressure by endothelin
nitric oxide mediated signal transduction
regulation of blood pressure
negative regulation of cell proliferation
response to heat
negative regulation of platelet activation
negative regulation of muscle hyperplasia
smooth muscle hyperplasia
removal of superoxide radicals
lung development
lipopolysaccharide-mediated signaling pathway
response to fluid shear stress
negative regulation of potassium ion transport
endothelial cell migration
cell redox homeostasis
positive regulation of angiogenesis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of blood vessel size
regulation of nitric-oxide synthase activity
negative regulation of hydrolase activity
negative regulation of calcium ion transport
signal transduction
response to estradiol
positive regulation of apoptotic process
negative regulation of smooth muscle cell proliferation
cellular response to mechanical stimulus
cellular response to transforming growth factor beta stimulus
response to hypoxia
female pregnancy
aging
response to mechanical stimulus
response to fructose
response to metal ion
response to lead ion
response to organic cyclic compound
response to activity
response to lipopolysaccharide
response to cytokine
cellular response to heat
response to statin
response to drug
response to amino acid
response to peptide hormone
response to ethanol
response to axon injury
response to corticosterone
response to hyperoxia
response to lipoprotein particle
retina development in camera-type eye
bone development
cellular response to magnesium ion
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
cellular response to organic cyclic compound
response to candesartan
response to peptide
response to ketone
response to salt
