Invitrogen

hnRNP U Polyclonal Antibody

Name: hnRNP U Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143907

查看其他13个hnRNP U抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (16)

hnRNP U Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 16

hnRNP U Antibody (PA5-143907) in ICC/IF

Immunocytochemistry analysis of hnRNP U in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in hnRNP U Polyclonal antibody (Product # PA5-143907) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropria... View More

hnRNP U Antibody in Immunohistochemistry (Paraffin) (IHC (P))

hnRNP U Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image hnRNP U Polyclonal Antibody

产品信息

PA5-143907

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human hnRNP U/p120/HNRNPU recombinant protein (Position: E262-L563).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075121

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human MOLT-4 whole cell, human Daudi whole cell, human HEL whole cell, human U251 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human breast cancer tissue, human gastric carcinoma tissue, human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human esophageal squamous carcinoma tissue, human thyroid cancer tissue, human spleen tissue, mouse brain tissue, rat brain tissue. ICC/IF: SiHa cell. Flow: HEL cell, C6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ30202; FLJ37978; GRIP120; Heterogeneous nuclear ribonucleoprotein U; heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); heterogenous nuclear ribonucleoprotein U; hnRNP U; HNRNPU antisense RNA 1; NCRNA00201; nuclear matrix protein sp120; Nuclear p120 ribonucleoprotein; p120; p120 nuclear protein; pp120; SAF-A; scaffold attachment factor A; Scaffold-attachment factor A; SP120; system N1 Na+ and H+-coupled glutamine transporter; transporter protein; transporter protein; system N1 Na+ and H+-coupled glutamine transporter; U211

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基因别名: AA408410; AI256620; AL024194; AL024437; AW557595; C1orf199; C86794; hnRNP U; HNRNPU; HNRNPU-AS1; HNRPU; rCG_20317; SAF-A; SAFA; SN1; Sp120; U21.1

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UniProt ID: (Human) Q00839, (Rat) Q6IMY8, (Mouse) Q8VEK3

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Entrez Gene ID: (Human) 3192, (Rat) 117280, (Mouse) 51810

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功能