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Invitrogen

p300 Polyclonal Antibody

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p300 Polyclonal Antibody

Product Details

PA5-143448

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EP300. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.29 mg/mL

Amount

129 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2938443

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Positive control: A431 nuclear extract.

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; histone butyryltransferase p300; histone crotonyltransferase p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein isonicotinyltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; unnamed protein product

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Gene Aliases: A430090G16; A730011L11; KAT3B; MKHK2; p300; p300 HAT; RSTS2

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UniProt ID: (Human) Q09472, (Mouse) B2RWS6

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Entrez Gene ID: (Human) 2033, (Mouse) 328572

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Function(s)
transcription cofactor binding transcription coactivator binding p53 binding DNA binding chromatin binding damaged DNA binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding zinc ion binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity acetyltransferase activity transferase activity, transferring acyl groups ligand-dependent nuclear receptor binding chromatin DNA binding histone acetyltransferase activity (H3-K18 specific) histone H2B acetyltransferase activity histone acetyltransferase activity (H3-K27 specific) tau protein binding androgen receptor binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding peptide-lysine-N-acetyltransferase activity protein propionyltransferase activity pre-mRNA intronic binding STAT family protein binding peptide 2-hydroxyisobutyryltransferase activity peptide lactyltransferase (CoA-dependent) activity histone lactyltransferase (CoA-dependent) activity acetylation-dependent protein binding peptide crotonyltransferase activity peptide butyryltransferase activity histone crotonyltransferase activity histone butyryltransferase activity histone isonicotinyltransferase activity DNA-binding transcription factor binding histone H3K122 acetyltransferase activity histone H1K75 acetyltransferase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding antigen binding protein kinase binding glucocorticoid receptor binding peroxisome proliferator activated receptor binding bHLH transcription factor binding macromolecular complex binding SMAD binding mitogen-activated protein kinase binding histone reader activity protein antigen binding promoter-specific chromatin binding
Process(es)
autophagosome assembly negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis positive regulation of protein phosphorylation thigmotaxis behavioral defense response gluconeogenesis glycolytic process regulation of glycolytic process chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade heart development skeletal muscle tissue development learning or memory circadian rhythm lipid biosynthetic process response to xenobiotic stimulus response to glucose organ morphogenesis negative regulation of autophagy positive regulation of glycoprotein biosynthetic process positive regulation of gene expression regulation of mitochondrion organization positive regulation of neuron projection development positive regulation of muscle atrophy N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation peptidyl-lysine acetylation B cell differentiation platelet formation positive regulation of cell growth lung development positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of protein oligomerization positive regulation of collagen biosynthetic process cellular response to UV multicellular organism growth megakaryocyte development endodermal cell differentiation swimming TORC1 signaling TORC2 signaling positive regulation of protein import into nucleus intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator protein kinase B signaling response to estrogen positive regulation by host of viral transcription fat cell differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of axon extension positive regulation of cell size positive regulation of gene expression, epigenetic positive regulation of proteolysis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of protein secretion protein stabilization cartilage development response to calcium ion positive regulation of protein kinase B signaling canonical Wnt signaling pathway regulation of angiotensin metabolic process positive regulation of sarcomere organization face morphogenesis regulation of androgen receptor signaling pathway positive regulation of cell growth involved in cardiac muscle cell development peptidyl-lysine propionylation protein-DNA complex assembly protein localization to chromatin cellular response to leucine cellular response to interleukin-1 response to dexamethasone tricarboxylic acid metabolic process regulation of tubulin deacetylation peptidyl-lysine crotonylation peptidyl-lysine butyrylation DNA repair-dependent chromatin remodeling positive regulation of cellular response to hypoxia positive regulation of hydrogen peroxide-mediated programmed cell death positive regulation of protein acetylation negative regulation of chromosome condensation positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of T-helper 17 cell lineage commitment negative regulation of miRNA metabolic process regulation of nucleotide-excision repair negative regulation of apoptotic signaling pathway stimulatory C-type lectin receptor signaling pathway nervous system development regulation of autophagy regulation of cellular response to heat positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of brown fat cell differentiation
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