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Invitrogen
recA Recombinant Rabbit Monoclonal Antibody (PSH09-96)
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recA Antibody (MA570104) in ICC/IF
Immunocytochemistry analysis of recA in E.coli. Samples were incubated with recA monoclonal antibody (Product # MA570104) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was used ... View More
产品信息
MA570104
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH09-96
抗原
Recombinant protein within bacterium RecA aa 1-353.
偶联物
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: E.coli lysates.
Subcellular location: Cytoplasm.
靶标信息
E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Expression is induced by SOS response caused by DNA damage. The RecA protein has multiple functions, such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loops and Holliday structures during homologous recombination, and co-protease activities that promote self-cleavage of the LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its is homologous to the eukaryotic Rad51 protein and Dmcl protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: RAD51; RAD51A
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