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          • Primary Antibodies ›
          • uNOS Antibodies

          Invitrogen

          uNOS Polyclonal Antibody

          7个已发表图片
          35 篇参考文献

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          Cite uNOS Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (1)
          • 已发表图片 (7)
          uNOS Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          uNOS Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          图: 1 / 8

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          uNOS Antibody (PA1-039) in IHC

          Immunolocalization staining of NOS in drosophila optic nerve using Product # PA1-039. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          uNOS Polyclonal Antibody

          产品信息

          PA1-039

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500
          查看8篇已发表文章 8篇已发表文章

          免疫组化 (IHC)

          -
          查看16篇已发表文章 16篇已发表文章

          免疫组化(冰冻) (IHC (F))

          1:100
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:100-1:200
          查看10篇已发表文章 10篇已发表文章
          产品规格

          种属反应

          Arthropod, Bovine, Fruit fly, Human, Mouse, Pig, Rat

          已发表种属

          Arthropod, Cnidarian, Crab, Fish, Fruit fly, Human, Insect, Mollusc, Mouse, Pig, Rabbit, Rat, Shark

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of murine iNOS and bNOS.
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          规格

          100 µg

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7.2-7.4, with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325776

          产品详细信息

          PA1-039 detects mouse macrophage inducible nitric oxide synthase (iNOS); rat, bovine, Drosophila, and porcine brain nitric oxide synthase (bNOS); human, porcine and bovine epithelial nitric oxide synthase (eNOS).

          PA1-039 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse macrophage iNOS; an ~155 kDa protein representing rat, bovine and porcine bNOS; ~140 kDa protein representing porcine and bovine eNOS. Immunohistochemical staining of NOS in Drosophila optic tissue with PA1-039 results in staining of the optic nerve. In immunohistochemistry procedures PA1-039 also stains NOS in human endothelial cells, macrophages, and peripheral neural tract cells.

          The PA1-039 immunogen is a synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of mouse iNOS and bNOS. This sequence is highly conserved among the different NOS isoforms.

          靶标信息

          NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: bNOS; cNOS; Constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; HEP-NOS; Hepatocyte NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, brain; Nitric oxide synthase, endothelial; Nitric oxide synthase, inducible; nitric-oxide synthase; nNOS; NOS type I; NOS type II; NOS type III; NOSII; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; Peptidyl-cysteine S-nitrosylase NOS2

          查看更多 收起

          基因别名: 2310005C01Rik; 2310065A03Rik; Bnos; cNOS; EC-NOS; Ecnos; eNOS; i-NOS; INOS; Inosl; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS1; NOS2; NOS2A; NOS3; NOSIII

          查看更多 收起

          UniProt ID: (Bovine) Q27995, (Pig) P79290, (Pig) Q28969, (Rat) P29476, (Mouse) Q9Z0J4, (Mouse) P29477, (Rat) Q06518, (Mouse) P70313, (Rat) Q62600

          查看更多 收起

          Entrez Gene ID: (Pig) 100520934, (Bovine) 282876, (Pig) 396859, (Bovine) 287024, (Pig) 397557, (Rat) 24598, (Mouse) 18125, (Mouse) 18126, (Rat) 24599, (Mouse) 18127, (Rat) 24600

          查看更多 收起

          功能
          nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding NADPH-hemoprotein reductase activity iron ion binding protein binding amino acid binding sodium channel regulator activity enzyme binding protein homodimerization activity ion channel binding cadmium ion binding ATPase binding scaffold protein binding actin binding receptor binding beta-catenin binding cAMP-dependent protein kinase regulator activity oxidoreductase activity protein kinase binding tetrahydrobiopterin binding arginine binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding actin monomer binding oxidoreductase protein modifying enzyme
          参与通路
          nitric oxide biosynthetic process peptidyl-cysteine S-nitrosylation prostaglandin secretion interleukin-6 production interleukin-8 production oxidation-reduction process regulation of cytokine production involved in inflammatory response arginine catabolic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion positive regulation of blood vessel diameter response to hypoxia regulation of sodium ion transport muscle contraction striated muscle contraction nitric oxide mediated signal transduction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to heat response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity response to nutrient levels response to lipopolysaccharide negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport ovulation from ovarian follicle superoxide metabolic process inflammatory response negative regulation of gene expression cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of protein kinase activity regulation of insulin secretion positive regulation of killing of cells of other organism cellular response to lipopolysaccharide cellular response to interferon-gamma endothelial cell proliferation blood vessel remodeling signal transduction G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger regulation of blood pressure response to mechanical stimulus response to hormone glycoside metabolic process cGMP-mediated signaling response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing positive regulation of apoptotic process retinoic acid receptor signaling pathway defense response to Gram-negative bacterium cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state response to dexamethasone angiogenesis in utero embryonic development regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin negative regulation of muscle hyperplasia smooth muscle hyperplasia lung development lipopolysaccharide-mediated signaling pathway endothelial cell migration positive regulation of angiogenesis negative regulation of smooth muscle cell proliferation regulation of blood vessel size cellular response to transforming growth factor beta stimulus response to fructose response to metal ion response to cytokine response to fluid shear stress cellular response to heat response to statin response to drug response to amino acid response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
          It has to be done as per old AB suggested Products section.

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