{"id":1023,"date":"2026-03-19T07:08:26","date_gmt":"2026-03-19T07:08:26","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1023"},"modified":"2026-04-22T21:59:29","modified_gmt":"2026-04-22T21:59:29","slug":"complete-guide-to-cell-proliferation","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/complete-guide-to-cell-proliferation\/","title":{"rendered":"\u7ec6\u80de\u589e\u6b96\u5168\u653b\u7565\uff0c\u7cbe\u51c6\u6355\u83b7\u7ec6\u80de\u52a8\u6001"},"content":{"rendered":"\n

\u7ec6\u80de\u589e\u6b96\u6982\u5ff5\u53ca\u7ec6\u80de\u5468\u671f\u4ecb\u7ecd<\/strong><\/h2>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u56fe1. \u7ec6\u80de\u5468\u671f\u793a\u610f\u56fe<\/p>\n\n\n\n

\u5e38\u89c1\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/h2>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u56fe2. PrestoBlue HS & AlamaBlue HS\u53cd\u5e94\u539f\u7406\uff08\u4e0a\uff09\u53ca\u5b9e\u9a8c\u7ed3\u679c\u56fe\uff08\u4e0b\uff091<\/sup><\/p>\n\n\n\n

DNA\u542b\u91cf\u68c0\u6d4b\u6cd5\uff1aDNA\u7684\u7cbe\u51c6\u52a8\u6001\u89e3\u6790<\/strong><\/h3>\n\n\n\n

\u901a\u8fc7\u91cf\u5316\u7ec6\u80de\u5185DNA\u542b\u91cf\u8bc4\u4f30\u7ec6\u80de\u589e\u6b96\u72b6\u6001\u3002\u5e38\u89c1\u8bd5\u5242\u4e3aCyQUANT\u2122 NF\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08\u603b\u7ec6\u80de\u8ba1\u6570\uff09\u548cCyQUANT\u2122 \u76f4\u63a5\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08\u6d3b\u7ec6\u80de\u8ba1\u6570\uff09\u3002\u8bd5\u5242\u76d2\u5185\u7684\u7ec6\u80de\u901a\u900f\u6027\u67d3\u6599\u80fd\u591f\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\u6838\uff0c\u4e0eDNA\u7ed3\u5408\u540e\u53d1\u51fa\u8367\u5149\uff0c\u901a\u8fc7\u4e0e\u5df2\u77e5\u7ec6\u80de\u6570\u7684\u6807\u51c6\u66f2\u7ebf\u5bf9\u6bd4\uff0c\u786e\u5b9a\u7ec6\u80de\u5185\u7684\u6838\u9178\u542b\u91cf\uff0c\u4ece\u800c\u53cd\u6620\u7ec6\u80de\u6570\u91cf\u3002\u4e0e\u7ecf\u5178\u7684CyQUANT\u2122 \u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u8bd5\u5242\u76d2\u76f8\u6bd4\uff0c\u8fd9\u4e24\u79cd\u8bd5\u5242\u76d2\u65e0\u9700\u88c2\u89e3\u7ec6\u80de\uff0c\u8be5\u7c7b\u65b9\u5177\u6709\u8d85\u9ad8\u7075\u654f\u5ea6\uff0c\u9002\u914d\u81ea\u52a8\u5316\u5de5\u4f5c\u7ad9\uff0c\u66f4\u9002\u5408\u5feb\u901f\u8bc4\u4f30\u5242\u91cf-\u6548\u5e94\u5173\u7cfb\u7684\u6297\u80bf\u7624\u836f\u7269\u7b5b\u9009<\/strong>\u4ee5\u53ca3D\u7ec6\u80de\u57f9\u517b\u4e0e\u7ec4\u7ec7\u5de5\u7a0b\u4e2d\u7684\u7ec6\u80de\u589e\u6b96\u5206\u6790<\/strong>\u7b49\u65b9\u5411\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe3. CyQUANT \u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u8bd5\u5242\u76d2\u5de5\u4f5c\u6d41\u7a0b\uff08\u5de6\uff09\u53ca\u5bf9\u5e94\u7684\u68c0\u6d4b\u4fe1\u53f7\u7ebf\u6027\u56fe\uff08\u53f3\uff09<\/p>\n\n\n\n

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\u901a\u8fc7\u6807\u8bb0\u65b0\u5408\u6210\u7684DNA\u6765\u8bc4\u4f30\u7ec6\u80de\u589e\u6b96\u3002\u76ee\u524d\u5e38\u7528\u7684\u65b9\u6cd5\u662fEdU\uff085-\u4e59\u7094\u57fa-2′-\u8131\u6c27\u5c3f\u82f7\uff09\u63ba\u5165\u6cd5\u3002EdU\u4f5c\u4e3a\u80f8\u82f7\u7c7b\u4f3c\u7269\uff0c\u53ef\u4ee5\u5728DNA\u5408\u6210\u8fc7\u7a0b\u4e2d\u63ba\u5165\u5230\u65b0\u751fDNA\u4e2d\uff0c\u672b\u7aef\u88f8\u9732\u51fa\u7684\u7094\u70c3\u80fd\u591f\u4e0e\u53e0\u6c2e\u57fa\u4fee\u9970\u7684\u8367\u5149\u67d3\u6599\u901a\u8fc7\u70b9\u51fb\u5316\u5b66\u53cd\u5e94\u7279\u5f02\u6027\u7ed3\u5408\uff0c\u901a\u8fc7\u6210\u50cf\u6216\u6d41\u5f0f\u65b9\u6cd5\u68c0\u6d4b\u8367\u5149\u4fe1\u53f7\uff0c\u4ece\u800c\u786e\u5b9a\u7ec6\u80de\u7684\u589e\u6b96\u60c5\u51b5\u3002\u4e0e\u4f20\u7edf\u5e95\u7269BrdU\u76f8\u6bd4\uff0cEdU\u65b9\u6cd5\u64cd\u4f5c\u66f4\u4e3a\u7b80\u4fbf\u4e14\u4e0d\u9700\u8981\u53d8\u6027DNA\u3002\u8be5\u7c7b\u65b9\u6cd5\u7075\u654f\u5ea6\u9ad8\u3001\u7279\u5f02\u6027\u597d\uff0c\u53ef\u7528\u4e8e\u6d3b\u4f53\u5b9e\u9a8c<\/strong>\uff0c\u4e5f\u80fd\u591f\u534f\u540c\u5176\u4ed6\u6838\u9178\u67d3\u6599\u7279\u5f02\u6027\u68c0\u6d4b\u7ec6\u80deS\u671f<\/strong>\uff0c\u5e38\u7528\u4e8e\u8bc4\u4f30\u836f\u7269\u5bf9DNA\u590d\u5236\u5f71\u54cd<\/strong>\u3001\u8ffd\u8e2a\u7ec6\u80de\u7684\u589e\u6b96\u5206\u5316<\/strong>\u548c\u65b0\u795e\u7ecf\u5143\u7684\u751f\u6210\u7b49<\/strong>\u7814\u7a76\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u56fe5. \u67d3\u6599\u7a00\u91ca\u6d4b\u91cf\u7ec6\u80de\u5206\u88c2\u539f\u7406\uff08\u5de6\uff09\u53caCellTrace\u7528\u4e8e\u68c0\u6d4bPBMC\u523a\u6fc0\u540e\u7684\u589e\u6b96\u60c5\u51b5\uff08\u53f3\uff09<\/p>\n\n\n\n

\u86cb\u767d\u68c0\u6d4b\u6cd5\uff1a\u5206\u5b50\u6807\u8bb0\u7684\u7cbe\u51c6\u6355\u6349<\/strong><\/h3>\n\n\n\n

\u901a\u8fc7\u68c0\u6d4b\u4e0e\u7ec6\u80de\u589e\u6b96\u76f8\u5173\u7684\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u91cf\u6765\u8bc4\u4f30\u7ec6\u80de\u589e\u6b96\u60c5\u51b5\u3002\u5e38\u7528\u7684\u86cb\u767d\u8d28\u6807\u8bb0\u5305\u62ecKi-67\u548cPCNA\uff08\u589e\u6b96\u7ec6\u80de\u6838\u6297\u539f\uff09\uff0cKi-67\u5728\u9664G0\u671f\u5916\u7684\u6240\u6709\u5468\u671f\u9636\u6bb5\u8868\u8fbe\uff0cPCNA\u5219\u53c2\u4e0eDNA\u590d\u5236\u3002\u8be5\u7c7b\u65b9\u6cd5\u53ef\u7ed3\u5408\u5176\u4ed6\u7ec6\u80de\u6807\u8bb0\u8fdb\u884c\u591a\u8272\u5206\u6790\uff0c\u5e38\u7528\u4e8e\u4fe1\u53f7\u901a\u8def\u86cb\u767d\u7684\u68c0\u6d4b<\/strong>\u3001\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790<\/strong>\u7b49\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe6. HeLa\u91ce\u751f\u578b\u7ec6\u80de\u3001HeLa Cas9\u5bf9\u7167\u7ec6\u80de\u548cHeLa Ki-67\u6572\u9664\u7ec6\u80de\u7684Ki-67\u514d\u75ab\u8367\u5149\u5206\u6790\uff08\u5de6\uff09\u4eba\u7c7b\u7ed3\u80a0\uff08\u795e\u7ecf\u5185\u5206\u6ccc\u80bf\u7624\uff09\u7ec4\u7ec7\u5207\u7247PCNA\u7684\u514d\u75ab\u7ec4\u5316\u5206\u6790\uff08\u53f3\uff09<\/p>\n\n\n\n

\u4e8b\u5b9e\u4e0a\u968f\u7740\u68c0\u6d4b\u6280\u672f\u7684\u53d1\u5c55\uff0c\u591a\u89d2\u5ea6\u8bc4\u4f30\u7ec6\u80de\u589e\u6b96\u60c5\u51b5\u6210\u4e3a\u8d8b\u52bf\uff0c\u4e0d\u540c\u65b9\u6cd5\u76f8\u4e92\u9a8c\u8bc1\u63d0\u9ad8\u4e86\u7ed3\u679c\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\uff0c\u591a\u53c2\u6570\u5206\u6790\u4e5f\u6709\u52a9\u4e8e\u63ed\u793a\u7ec6\u80de\u589e\u6b96\u590d\u6742\u7684\u751f\u7269\u73b0\u8c61\uff0c\u56e0\u6b64\u5982\u679c\u9700\u8981\u5bf9\u7ec6\u80de\u589e\u6b96\u8fdb\u884c\u6df1\u5165\u548c\u5168\u9762\u5206\u6790\u7684\u7814\u7a76\uff0c\u53ef\u4ee5\u8003\u8651\u591a\u79cd\u68c0\u6d4b\u65b9\u6cd5\u8054\u5408\u5e94\u7528\u3002<\/strong><\/p>\n\n\n\n

\u5e38\u89c1\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u65b9\u6cd5\u6c47\u603b<\/strong><\/h2>\n\n\n\n
\u68c0\u6d4b\u65b9\u6cd5<\/td>\u4eea\u5668\u5e73\u53f0<\/td>\u70ed\u95e8\u8bd5\u5242<\/td>\u63a8\u8350\u8d27\u53f7<\/td>\u89c4\u683c<\/td><\/td><\/td><\/tr>
\u6d3b\u529b\u68c0\u6d4b\u6cd5<\/td>\u9176\u6807\u4eea<\/td>PrestoBlue HS\u9ad8\u7eaf\u5ea6\u7ec6\u80de\u6d3b\u529b\u68c0\u6d4b\u8bd5\u5242<\/td>P50200<\/a><\/td>25mL<\/td><\/td><\/td><\/tr>
\u6d3b\u529b\u68c0\u6d4b\u6cd5<\/td>\u9176\u6807\u4eea<\/td>AlamarBlueHS \u9ad8\u7eaf\u5ea6\u7ec6\u80de\u6d3b\u529b\u68c0\u6d4b\u8bd5\u5242<\/td>A50100<\/a><\/td>25mL<\/td><\/td><\/td><\/tr>
DNA\u542b\u91cf\u68c0\u6d4b\u6cd5<\/td>\u9176\u6807\u4eea<\/td>CyQUANTM<\/sup> Direct
\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b<\/td>		C35011<\/a><\/td>10x96T<\/td><\/td><\/td><\/tr>
DNA\u542b\u91cf\u68c0\u6d4b\u6cd5<\/td>\u9176\u6807\u4eea<\/td>CyQUANTTM<\/sup> NF
\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b<\/td>		C35007<\/a><\/td>200T<\/td><\/td><\/td><\/tr>
DNA\u542b\u91cf\u68c0\u6d4b\u6cd5<\/td>\u9176\u6807\u4eea<\/td>CyQUANTT
\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b<\/td>		C7026<\/a><\/td>1000T<\/td><\/td><\/td><\/tr>
\u65b0\u751fDNA\u68c0\u6d4b\u6cd5<\/td>\u6210\u50cf\/\u6d41\u5f0f<\/td>Click-iTr EdU
\u7ec6\u80de\u589e\u6b96\u6210\u50cf\u8bd5\u5242\u76d2<\/td>		C10337<\/a><\/td>1 kit<\/td><\/td><\/td><\/tr>
\u65b0\u751fDNA\u68c0\u6d4b\u6cd5<\/td>\u6210\u50cf\/\u6d41\u5f0f<\/td>Click-iT Plus EdU
\u7ec6\u80de\u589e\u6b96\u6210\u50cf\u8bd5\u5242\u76d2<\/td>		C10637<\/a><\/td>1 kit<\/td><\/td><\/td><\/tr>
\u67d3\u6599\u7a00\u91ca\u6cd5<\/td>\u6d41\u5f0f<\/td>eBioscience CFSE
\u7ec6\u80de\u589e\u6b96\u8bd5\u5242\u76d2<\/td>		65-0850-84<\/a><\/td>5x500ug<\/td><\/td><\/td><\/tr>
\u67d3\u6599\u7a00\u91ca\u6cd5<\/td>\u6d41\u5f0f<\/td>CellTrace Violet
\u7ec6\u80de\u589e\u6b96\u8bd5\u5242\u76d2<\/td>		C34571<\/a><\/td>20T<\/td><\/td><\/td><\/tr>
\u86cb\u767d\u68c0\u6d4b\u6cd5<\/td>\u6210\u50cf<\/td>Ki-67\u6297\u4f53<\/td>740008T<\/a><\/td>100 uL<\/td><\/td><\/td><\/tr>
\u86cb\u767d\u68c0\u6d4b\u6cd5<\/td>\u6210\u50cf<\/td>PCNA\u6297\u4f53<\/td>MA5-32051<\/a><\/td>100 uL<\/td><\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

FAQs<\/strong><\/h2>\n\n\n\n

Q<\/strong> \u54ea\u79cd\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u65b9\u6cd5\u53ef\u4ee5\u7528\u4e8e3D\u57f9\u517b\u4f53\u7cfb\uff1f<\/strong><\/p>\n\n\n\n

A<\/strong> PrestoBlue HS\u7ec6\u80de\u6d3b\u529b\u8bd5\u5242\u3001CyQUANT\u2122<\/sup>\u00a0XTT\u548cCyQUANT\u2122 Direct\u5df2\u88ab\u9a8c\u8bc1\u8fc7\u53ef\u7528\u4e8e3D\u57f9\u517b\u4f53\u7cfb\u3002<\/p>\n\n\n\n

Q<\/strong> \u5bf9\u4e8e\u5177\u6709\u591a\u4e2a\u65f6\u95f4\u70b9\u7684\u9ad8\u901a\u91cf\u68c0\u6d4b\uff0c\u6211\u5e94\u8be5\u9009\u62e9\u54ea\u79cd\u68c0\u6d4b\u65b9\u6cd5\uff1f<\/strong><\/p>\n\n\n\n

A<\/strong> \u5bf9\u4e8e\u5177\u6709\u591a\u4e2a\u65f6\u95f4\u70b9\u6216\u8f83\u957f\u5b75\u80b2\u65f6\u95f4\u7684\u5b9e\u9a8c\uff0c\u6211\u4eec\u5efa\u8bae\u4f7f\u7528PrestoBlue HS\u53caAlamarBlue HS\u7ec6\u80de\u6d3b\u529b\u8bd5\u5242\u3002\u6ce8\u610f\u9700\u8981\u5728\u6bcf\u4e2a\u5b75\u80b2\u671f\u7ed3\u675f\u65f6\u7684\u65f6\u95f4\u70b9\u6dfb\u52a0\u8bd5\u5242\uff0c\u800c\u975e\u8fde\u7eed\u6d4b\u91cf\u3002\u8fd9\u79cd\u65b9\u5f0f\u53ef\u4ee5\u9632\u6b62\u8bd5\u5242\u88ab\u4ee3\u8c22\u6d3b\u8dc3\u7684\u7ec6\u80de\u8f6c\u5316\uff0c\u4ece\u800c\u5f71\u54cd\u7ed3\u679c\u3002<\/p>\n\n\n\n

\"contact<\/a><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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