{"id":1058,"date":"2026-03-24T08:52:12","date_gmt":"2026-03-24T08:52:12","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1058"},"modified":"2026-04-22T21:59:28","modified_gmt":"2026-04-22T21:59:28","slug":"caspase","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/caspase\/","title":{"rendered":"\u89e3\u8c1c\u7ec6\u80de\u51cb\u4ea1-Caspase\u7684\u795e\u79d8\u529b\u91cf"},"content":{"rendered":"\n

\u7ec6\u80de\u51cb\u4ea1\u6982\u89c8<\/strong><\/h2>\n\n\n\n

\u7ec6\u80de\u51cb\u4ea1\u662f\u4e00\u79cd\u7a0b\u5e8f\u6027\u7ec6\u80de\u6b7b\u4ea1\u8fc7\u7a0b\uff0c\u901a\u8fc7\u4e25\u683c\u8c03\u63a7\u7684\u7ec6\u80de\u5185\u4fe1\u53f7\u4f20\u5bfc\u673a\u5236\u6267\u884c\u3002\u8be5\u8fc7\u7a0b\u5bf9\u4e8e\u7ef4\u6301\u7ec4\u7ec7\u7a33\u6001\u548c\u6b63\u5e38\u53d1\u80b2\u81f3\u5173\u91cd\u8981\u3002\u5f02\u5e38\u7684\u7ec6\u80de\u51cb\u4ea1\u673a\u5236\u53ef\u80fd\u5bfc\u81f4\u591a\u79cd\u75be\u75c5\uff0c\u5982\u7ec6\u80de\u51cb\u4ea1\u8fc7\u5ea6\u4f1a\u5f15\u8d77\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\uff0c\u800c\u7ec6\u80de\u51cb\u4ea1\u4e0d\u8db3\u5219\u4e0e\u764c\u75c7\u7684\u53d1\u751f\u548c\u53d1\u5c55\u5bc6\u5207\u76f8\u5173\u3002\u5728\u51cb\u4ea1\u8fc7\u7a0b\u4e2d\uff0c\u7ec6\u80de\u4f1a\u7ecf\u5386\u4e00\u7cfb\u5217\u6807\u5fd7\u6027\u4e8b\u4ef6\uff0c\u4ece\u6700\u521d\u7684\u4fe1\u53f7\u63a5\u6536\u5230\u6700\u7ec8\u7684\u7ec6\u80de\u89e3\u4f53\uff0c\u6bcf\u4e00\u6b65\u90fd\u81f3\u5173\u91cd\u8981\u3002\u7406\u89e3\u8fd9\u4e9b\u8fc7\u7a0b\u4e0d\u4ec5\u6709\u52a9\u4e8e\u63ed\u793a\u75be\u75c5\u673a\u5236\uff0c\u8fd8\u80fd\u4e3a\u65b0\u7684\u6cbb\u7597\u65b9\u6cd5\u63d0\u4f9b\u601d\u8def\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe1. \u7ec6\u80de\u51cb\u4ea1\u8fdb\u7a0b\u56fe[1]<\/sup><\/p>\n\n\n\n

\u7ec6\u80de\u51cb\u4ea1\u8bf1\u5bfc\u9014\u5f84<\/strong><\/h2>\n\n\n\n

\u7ec6\u80de\u51cb\u4ea1\u7684\u8bf1\u5bfc\u9014\u5f84\u4e3b\u8981\u5305\u62ec\u5916\u6e90\u6027\uff08Extrinsic\uff09<\/strong>\u548c\u5185\u6e90\u6027\uff08Intrinsic\uff09<\/strong>\u4e24\u79cd\u3002<\/p>\n\n\n\n

\u5916\u6e90\u6027\u9014\u5f84<\/strong>\uff08\u6b7b\u4ea1\u53d7\u4f53\u9014\u5f84\uff09\u7531\u7ec6\u80de\u5916\u90e8\u4fe1\u53f7\u8bf1\u5bfc\uff0c\u901a\u8fc7\u6b7b\u4ea1\u53d7\u4f53\u4ecb\u5bfc\u3002\u5173\u952e\u5206\u5b50\u5305\u62ecFas\u53d7\u4f53\u548c\u80bf\u7624\u574f\u6b7b\u56e0\u5b50\u53d7\u4f53\uff08TNFR\uff09\u3002\u5f53\u914d\u4f53\uff08\u5982FasL\u6216TNF-\u03b1\uff09\u4e0e\u6b7b\u4ea1\u53d7\u4f53\u7ed3\u5408\u65f6\uff0c\u5f62\u6210\u6b7b\u4ea1\u8bf1\u5bfc\u4fe1\u53f7\u590d\u5408\u7269\uff08DISC\uff09\uff0cDISC\u6fc0\u6d3bCaspase-8\uff0c\u968f\u540e\u6fc0\u6d3b\u4e0b\u6e38\u6548\u5e94Caspase\uff08\u5982Caspase-3\uff09\uff0c\u5f15\u53d1\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n\n\n\n

\u5185\u6e90\u6027\u9014\u5f84<\/strong>\uff08\u7ebf\u7c92\u4f53\u9014\u5f84\uff09\u7531\u7ec6\u80de\u5185\u90e8\u4fe1\u53f7\uff08\u5982DNA\u635f\u4f24\u3001\u6c27\u5316\u5e94\u6fc0\uff09\u8bf1\u5bfc\uff0c\u4e3b\u8981\u901a\u8fc7\u7ebf\u7c92\u4f53\u4ecb\u5bfc\u3002\u5173\u952e\u5206\u5b50\u5305\u62ecBcl-2\u5bb6\u65cf\u86cb\u767d\u8d28\uff08\u5982Bax\u3001Bak\uff09\u3002\u4fc3\u51cb\u4ea1\u4fe1\u53f7\u5bfc\u81f4Bax\/Bak\u5728\u7ebf\u7c92\u4f53\u819c\u4e0a\u805a\u96c6\uff0c\u5f62\u6210\u5b54\u9053\uff0c\u91ca\u653e\u7ec6\u80de\u8272\u7d20c\u3002\u7ec6\u80de\u8272\u7d20c\u4e0eApaf-1\u548cCaspase-9\u5f62\u6210\u51cb\u4ea1\u5c0f\u4f53\uff0c\u6fc0\u6d3bCaspase-9\uff0c\u5e76\u8fdb\u4e00\u6b65\u6fc0\u6d3b\u4e0b\u6e38\u6548\u5e94Caspase\uff08\u6548\u5e94\u578b\u534a\u80f1\u5929\u51ac\u9176\uff09<\/strong>\uff0c\u6267\u884c\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe2. \u5185\u6e90\u6027\u9014\u5f84\u548c\u5916\u6e90\u6027\u9014\u5f84\u7ec6\u80de\u51cb\u4ea1[2]<\/sup><\/p>\n\n\n\n

Caspase\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/h2>\n\n\n\n

\u4f46\u65e0\u8bba\u5916\u6e90\u6216\u662f\u5185\u6e90\u6027\u9014\u5f84\u7ec6\u80de\u51cb\u4ea1\uff0c\u4e8c\u8005\u5747\u901a\u8fc7\u6fc0\u6d3b\u6548\u5e94Caspase\u542f\u52a8\u7ec6\u80de\u51cb\u4ea1\u8fc7\u7a0b\uff0c\u6548\u5e94Caspase\u662f\u53c2\u4e0e\u7ec6\u80de\u51cb\u4ea1\u8fc7\u7a0b\u7684\u5173\u952e\u9176\u7c7b\uff0c\u4e3b\u8981\u5305\u62ecCaspase 3\/7\uff0c\u56e0\u6b64\uff0c\u68c0\u6d4bCaspase 3\/7\u7684\u6d3b\u6027\u5bf9\u786e\u8ba4\u7ec6\u80de\u662f\u5426\u53d1\u751f\u51cb\u4ea1\u3001\u836f\u7269\u7b5b\u9009\u548c\u75be\u75c5\u7814\u7a76\u975e\u5e38\u91cd\u8981\u3002<\/p>\n\n\n\n

\u5e38\u7528\u68c0\u6d4b\u65b9\u6cd5\u6839\u636e\u5b9e\u9a8c\u6837\u672c\u7684\u4e0d\u540c\uff0c\u53ef\u4ee5\u5206\u4e3a\u5747\u8d28\u6cd5\u3001FLICA\uff08Fluorochrome-Labeled Inhibitors of Caspases\uff09\u3001\u8367\u5149\u5e95\u7269\u6cd5\u3001\u57fa\u4e8e\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\uff08FRET\uff0cFluorescence Resonance Energy Transfer\uff09\u7684\u68c0\u6d4b\u65b9\u6cd5\u4ee5\u53ca\u6297\u4f53\u68c0\u6d4b\u6cd5\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u5e2e\u52a9\u7814\u7a76\u7ec6\u80de\u51cb\u4ea1\u7684\u673a\u5236\u548c\u8fc7\u7a0b\u3002<\/p>\n\n\n\n

CellEvent Caspase 3\/7\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/h2>\n\n\n\n

CellEvent Caspase 3\/7\u68c0\u6d4b\u65b9\u6cd5\u57fa\u4e8e\u8367\u5149\u5e95\u7269\u6cd5\uff0c\u5e95\u7269\u4e2d\u5305\u542bDEVD\u80bd\u5e8f\u5217\uff0c\u5f53\u9176\u88ab\u6fc0\u6d3b\u65f6\uff0c\u8be5\u80bd\u5e8f\u5217\u88ab\u5207\u5272\uff0c\u91ca\u653e\u51fa\u5f3a\u8367\u5149\u4fe1\u53f7\u3002\u901a\u8fc7\u68c0\u6d4b\u8367\u5149\u5f3a\u5ea6\u7684\u53d8\u5316\uff0c\u53ef\u4ee5\u8bc4\u4f30Caspase 3\/7\u7684\u6d3b\u6027\u3002\u4e0e\u4f20\u7edfCaspase\u68c0\u6d4b\u65b9\u6cd5\u76f8\u6bd4\uff0c\u8fd9\u79cd\u68c0\u6d4b\u65b9\u6cd5\u5bf9\u7ec6\u80de\u7684\u51cb\u4ea1\u8fc7\u7a0b\u6ca1\u6709\u6291\u5236\u4f5c\u7528\uff0c\u65e0\u9700\u6e05\u6d17\uff0c\u6709\u52a9\u4e8e\u5b9e\u65f6\u76d1\u6d4b\u7ec6\u80de\u51cb\u4ea1\u3002\u5b83\u517c\u5bb9\u6d3b\u7ec6\u80de\u6210\u50cf\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\u68c0\u6d4b\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u9002\u7528\u4e8e\u591a\u79cd\u5b9e\u9a8c\u9700\u6c42\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe3. CellEvent Caspase3\/7\u68c0\u6d4b\u539f\u7406\u53caWorkflow<\/p>\n\n\n\n

\u6570\u636e\u5b9e\u4f8b[3]<\/sup><\/strong><\/h2>\n\n\n\n

\u4e3a\u4e86\u7814\u7a76\u661f\u5f62\u5b62\u83cc\u7d20\u5bf9\u51cb\u4ea1\u7684\u8bf1\u5bfc\u4f5c\u7528\uff0c\u6211\u4eec\u5bf9HeLa\u7ec6\u80de\u8fdb\u884c\u4e86\u957f\u65f6\u95f4\u7684\u8367\u5149\u76d1\u6d4b\u3002\u6211\u4eec\u5148\u540e\u752850 nM\u7684TMRM\u67d3\u6599\u548c5 \u00b5M\u7684CellEvent\u2122 Caspase-3\/7\u8bd5\u5242\u5bf9HeLa\u7ec6\u80de\u8fdb\u884c\u67d3\u8272\u3002\u968f\u540e\uff0c\u75280.5 \u00b5M\u7684\u661f\u5f62\u5b62\u83cc\u7d20\u5904\u7406\u7ec6\u80de\uff0c\u5e76\u6301\u7eed\u91c7\u96c67\u5c0f\u65f6\u7684\u56fe\u50cf\u3002\u5728\u8fd97\u5c0f\u65f6\u7684\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u4ee5\u660e\u663e\u89c2\u5bdf\u5230\u661f\u5f62\u5b62\u83cc\u7d20\u5f15\u8d77\u7684\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u4e27\u5931\u548ccaspase 3\/7\u7684\u6fc0\u6d3b\uff0c\u8fd9\u5206\u522b\u901a\u8fc7TMRM\u4fe1\u53f7\uff08\u7ea2\u8272\uff09\u7684\u51cf\u5c11\u548cCaspase 3\/7\u4fe1\u53f7\uff08\u7eff\u8272\uff09\u7684\u589e\u52a0\u5f97\u4ee5\u663e\u793a\uff08\u89c1\u56fe4\uff09\u3002\u6b64\u5916\uff0c\u7ed3\u5408\u4f7f\u7528CellEvent\u2122 Caspase-3\/7\u548c\u6b7b\u7ec6\u80de\u6838\u9178\u67d3\u6599PI\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u68c0\u6d4b\u51cb\u4ea1\u7ec6\u80de\u7684\u6bd4\u4f8b\uff08\u89c1\u56fe5\uff09\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe4. \u661f\u5f62\u5b62\u83cc\u7d20\u8bf1\u5bfc\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\uff08\u7ea2\uff09\u4e0b\u964d\u548cCaspase 3\/7\uff08\u7eff\uff09\u6fc0\u6d3b\uff0810x\uff0c5 min\/freq.\uff09<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe5. \u4e0d\u540c\u6d53\u5ea6\uff080 \u00b5M \u5de6\uff0c0.11 \u00b5M \u53f3\uff09\u661f\u5f62\u5b62\u83cc\u7d20\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u6d41\u5f0f\u56fe<\/p>\n\n\n\n

FAQs<\/strong><\/h2>\n\n\n\n

Q CellEvent Caspase-3\/7 Green Detection Reagent\u53ef\u4ee5\u7528\u4e8e\u56fa\u5b9a\u6837\u672c\u5417\uff1f<\/strong><\/p>\n\n\n\n

A<\/strong> \u4e0d\u53ef\u4ee5\uff0c\u8be5\u8bd5\u5242\u9002\u7528\u4e8e\u6d3b\u7ec6\u80de\u67d3\u8272\uff0c\u67d3\u8272\u540e\u53ef\u4ee5\u7528\u7532\u919b\u56fa\u5b9a\uff0c\u4e0d\u80fd\u76f4\u63a5\u67d3\u56fa\u5b9a\u6837\u672c\u3002<\/p>\n\n\n\n

Q CellEvent Caspase-3\/7 Green Detection Reagent\u67d3\u8272\u540e\u8fd8\u80fd\u505a\u514d\u75ab\u8367\u5149\u5b9e\u9a8c\u5417\uff1f<\/strong><\/p>\n\n\n\n

A<\/strong> \u53ef\u4ee5\uff0c\u63a8\u8350\u5148\u8fdb\u884c\u7ec6\u80de\u8868\u9762\u67d3\u8272\u548c\u672c\u4ea7\u54c1\u7684\u67d3\u8272\uff0c\u56fa\u5b9a\u540e\u518d\u8fdb\u884c\u5176\u4ed6\u7ec6\u80de\u5185\u7684\u67d3\u8272\u3002<\/p>\n\n\n\n

\u53c2\u8003\u8d44\u6599\u4e0e\u6587\u732e\uff1a<\/h2>\n\n\n\n

1. Invitrogen \u7ec6\u80de\u529f\u80fd\u5206\u6790\u4ea7\u54c1\u9009\u62e9\u6307\u5357<\/p>\n\n\n\n

2. Yuan, J., & Ofengeim, D. (2024). A guide to cell death pathways. Nature Reviews Molecular Cell Biology, 25(5), 379-395.<\/p>\n\n\n\n

3. \u201c\u51cb\u4ea1\u4f7f\u8005-Caspase\u5728\u51cb\u4ea1\u5206\u6790\u4e2d\u7684\u5e94\u7528\u201d \u8d5b\u9ed8\u98deCOT\u8bfe\u7a0b<\/p>\n\n\n\n

\"contact<\/a><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7ec6\u80de\u51cb\u4ea1\u6982\u89c8 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