{"id":1090,"date":"2026-03-20T06:16:43","date_gmt":"2026-03-20T06:16:43","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1090"},"modified":"2026-04-22T21:59:29","modified_gmt":"2026-04-22T21:59:29","slug":"ic-fixation-buffer","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/ic-fixation-buffer\/","title":{"rendered":"\u7ec6\u80de\u5185\u6d41\u5f0f\u67d3\u8272\u505a\u5f97\u597d\uff0c\u56fa\u5b9a\u7834\u819c\u4e0d\u80fd\u5c11\uff01"},"content":{"rendered":"\n

\u6d41\u5f0f\u7ec6\u80de\u672f\u4e2d\u5bf9\u7ec6\u80de\u5185\u7684\u86cb\u767d\u9776\u6807\u67d3\u8272\uff0c\u5f80\u5f80\u9700\u8981\u7528\u5230\u56fa\u5b9a\u5242\u548c\u7834\u819c\u5242\uff0c\u5176\u76ee\u7684\u662f\u4e3a\u4e86\u56fa\u5b9a\u7ec6\u80de\u5e76\u7ed9\u7ec6\u80de\u819c\u7a7f\u5b54\uff0c\u4ee5\u4fbf\u8fdb\u884c\u540e\u7eed\u7684\u67d3\u8272\u548c\u5206\u6790\u3002\u8fd9\u6837\u53ef\u4ee5\u4fdd\u6301\u7ec6\u80de\u7684\u5f62\u6001\u5b8c\u6574\u6027\uff0c\u4f7f\u5f97\u7ec6\u80de\u5185\u7684\u76ee\u6807\u86cb\u767d\u80fd\u591f\u66b4\u9732\u51fa\u6765\uff0c\u65b9\u4fbf\u67d3\u8272\u548c\u5206\u6790\uff0c\u5e76\u4e14\u80fd\u591f\u5bf9\u7ec6\u80de\u5185\u86cb\u767d\u8fdb\u4e00\u6b65\u7814\u7a76\u548c\u5206\u6790\u3002\u4f7f\u7528\u5408\u9002\u7684\u56fa\u5b9a\u7834\u819c\u5242\uff0c\u53ef\u4ee5\u5728\u56fa\u5b9a\u7834\u819c\u7684\u540c\u65f6\u4fdd\u7559\u7ec6\u80de\u819c\u86cb\u767d\u4e0a\u7ed3\u5408\u7684\u8367\u5149\u3002<\/p>\n\n\n\n

eBioscience\u2122 \u6d41\u5f0f\u80de\u5185\u56fa\u5b9a\u7834\u819c\u7f13\u51b2\u6db2\u4e3b\u8981\u5305\u542b\u4e24\u4e2a\u7ec4\u5206\uff1aIntracellular Fixation Buffer\uff0c125 mL\uff08\u5355\u72ec\u8d27\u53f700-8222-49<\/a>\uff09\uff0cPermeabilization Buffer\uff0810x\uff09\uff0c100 mL\uff08\u5355\u72ec\u8d27\u53f7 00-8333-56\uff09<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u8bd5\u5242\u914d\u7f6e<\/strong><\/h2>\n\n\n\n

Intracellular Fixation Buffer\uff1a\u901a\u5e38\u662f2X\uff0c\u53ef\u76f4\u63a5\u7b49\u4f53\u79ef\u52a0\u5165\u5f85\u56fa\u5b9a\u7684\u7ec6\u80de\u4f53\u7cfb\u4e2d\u3002<\/p>\n\n\n\n

Permeabilization Buffer\uff1a\u4ea7\u54c1\u4e3a10X\uff0c\u9700\u8981\u4f7f\u7528\u53bb\u79bb\u5b50\u6c34\u7a00\u91ca\u81f31X\u4f7f\u7528\u3002<\/p>\n\n\n\n

\u5728\u8fd9\u91cc\u63d0\u9192\u5927\u5bb6\u7684\u662f\uff0c\u68c0\u6d4b\u7ec6\u80de\u6838\u5185\u7684\u56e0\u5b50\u5efa\u8bae\u4f7f\u7528eBioscience\u2122 Foxp3\/\u8f6c\u5f55\u56e0\u5b50\u6d41\u5f0f\u56fa\u5b9a\u7834\u819c\u7f13\u51b2\u6db2\uff08\u8d27\u53f700-5523-00<\/a>\uff09\uff0c\u540e\u7eed\u6211\u4eec\u4e5f\u4f1a\u4ecb\u7ecd\u5173\u4e8e\u8be5\u4ea7\u54c1\u7684\u4f7f\u7528\u65b9\u6cd5\u3002<\/p>\n\n\n\n

\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/h2>\n\n\n\n

\u672c\u5b9e\u9a8c\u65b9\u6848\u662f\u4ee51.5mL EP\u7ba1\u4e3a\u4f8b<\/strong><\/h3>\n\n\n\n

1. [\u53ef\u9009]\u6839\u636e\u9700\u8981\u68c0\u6d4b\u7684\u80de\u5185\u86cb\u767d\uff0c\u9009\u62e9\u5408\u9002\u7684\u523a\u6fc0\u6761\u4ef6\u5bf9\u7ec6\u80de\u8fdb\u884c\u9884\u5904\u7406\u3002\u70b9\u51fb\u5b9e\u9a8c\u6b65\u9aa4\u6700\u540e\u7684\u5f80\u671f\u5185\u5bb9\uff1a\u7ec6\u80de\u56e0\u5b50\u6d41\u5f0f\u5b9e\u9a8c\u65b9\u6cd5\uff0c\u4e86\u89e3\u66f4\u591a\u3002
2. \u5236\u5907\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u5c06\u51c6\u5907\u597d\u768410^6\u4e2a\u7ec6\u80de\u7f6e\u4e8e100 \u03bcL PBS\u4e2d\u3002
3. [\u53ef\u9009]\u6839\u636e\u6837\u672c\u79cd\u5c5e\u53ca\u6765\u6e90\u9009\u62e9\u5408\u9002\u7684Fc\u53d7\u4f53\u5242\u8fdb\u884c\u5c01\u95ed\u3002\u70b9\u51fb\u5b9e\u9a8c\u6b65\u9aa4\u6700\u540e\u7684\u5f80\u671f\u5185\u5bb9\uff1a\u5c01\u95edFc\u53d7\u4f53\uff0c\u4e86\u89e3\u66f4\u591a\u3002
4. [\u53ef\u9009]\u4f7f\u7528\u53ef\u56fa\u5b9a\u7ec6\u80de\u6d3b\u6027\u67d3\u6599\u5bf9\u7ec6\u80de\u8fdb\u884c\u67d3\u8272\uff1a\u4f7f\u75281 mL PBS\u91cd\u60ac\u7ec6\u80de\uff0c\u5411\u5176\u4e2d\u52a0\u51651 \u03bcL\u53ef\u56fa\u5b9a\u6b7b\u6d3b\u67d3\u6599\uff0c\u6df7\u5408\u5747\u5300\u540e4\u00b0C\u907f\u5149\u5b75\u80b230 min\u3002
5. [\u53ef\u9009]\u5bf9\u7ec6\u80de\u8868\u9762\u6807\u5fd7\u7269\u8fdb\u884c\u67d3\u8272\uff1a\u4f7f\u7528Staining Buffer\u914d\u5236\u8868\u9762\u6297\u4f53\u9884\u6df7\u6db2\uff0c\u6bcf\u4e2a\u6837\u672c\u9700\u898150 \u03bcL\u7684\u6297\u4f53\u9884\u6df7\u6db2\uff0c\u4f7f\u67d3\u8272\u4f53\u7cfb\u603b\u4f53\u79ef\u7ea6\u4e3a100 \u03bcL\uff0c\u8f7b\u8f7b\u5730\u8109\u51b2\u6da1\u65cb\u6df7\u5408\uff0c4\u00b0C\u907f\u5149\u5b75\u80b230 min\u3002 \u70b9\u51fb\u5b9e\u9a8c\u6b65\u9aa4\u6700\u540e\u7684\u5f80\u671f\u5185\u5bb9\uff1a\u7ec6\u80de\u8868\u9762\u6d41\u5f0f\u67d3\u8272\uff0c\u4e86\u89e3\u66f4\u591a\u3002
6. \u6700\u540e\u4e00\u6b21\u6d17\u6da4\u540e\uff0c\u5f03\u4e0a\u6e05\u5e76\u8109\u51b2\u6da1\u65cb\u6837\u54c1\u4ee5\u5b8c\u5168\u79bb\u89e3\u7ec6\u80de\u56e2\u5757\uff0c\u901a\u5e38\u4fdd\u7559\u7ea6100 \u03bcL\u6b8b\u4f59\u4f53\u79ef\u3002
7. \u6dfb\u52a0100 \u03bcL IC\u56fa\u5b9a\u6db2\u6765\u56fa\u5b9a\u7ec6\u80de\uff0c\u4e4b\u540e\u8109\u51b2\u6da1\u65cb\u6df7\u5408\u3002
8. \u5ba4\u6e29\u6761\u4ef6\u4e0b\u907f\u5149\u5b75\u80b220-60\u5206\u949f\u3002
9. \u52a0\u51651 mL 1X\u7834\u819c\u6db2\uff0c\u5728\u5ba4\u6e29\u4e0b400-600 x g \u79bb\u5fc35\u5206\u949f\u3002
10. \u5f03\u4e0a\u6e05\uff0c\u91cd\u590d\u4e0a\u4e00\u6b65\u3002
11. \u5c06\u7ec6\u80de\u6c89\u6dc0\u91cd\u60ac\u4e8e100 \u03bcL 1X\u7834\u819c\u6db2\u4e2d\uff0c\u76f4\u63a5\u52a0\u5165\u63a8\u8350\u91cf\u7684\u76f4\u6807\u4e00\u6297\u4ee5\u68c0\u6d4b\u7ec6\u80de\u5185\u6297\u539f\uff0c\u5e76\u5728\u5ba4\u6e29\u4e0b\u907f\u5149\u5b75\u80b220-60\u5206\u949f\u3002
12. \u52a0\u51651 mL 1X\u7834\u819c\u6db2\uff0c\u5ba4\u6e29\u4e0b400-600 x g\u79bb\u5fc35\u5206\u949f\u3002
13. \u5f03\u4e0a\u6e05\uff0c\u91cd\u590d\u4e0a\u4e00\u6b65\u3002
14. \u5c06\u67d3\u8272\u540e\u7684\u7ec6\u80de\u91cd\u60ac\u4e8e\u9002\u5f53\u4f53\u79ef\u7684\u6d41\u5f0f\u7ec6\u80de\u67d3\u8272\u6db2\u4e2d\uff0c\u4e0a\u673a\u68c0\u6d4b\u3002
15. \u7ed3\u679c\u5206\u6790\uff1a\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5708\u95e8\u89c4\u5219\u5206\u6790\u86cb\u767d\u8868\u8fbe\u60c5\u51b5\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7ed3\u679c\u5206\u6790\u4e3e\u4f8b\uff1a\u901a\u8fc7\u6d41\u5f0f\u591a\u8272\u67d3\u8272\uff0c\u68c0\u6d4b\u7ecf\u523a\u6fc0\u540eT\u7ec6\u80de\u4e2dIFN-\u03b3\uff0cTNF-\u03b1\u548cIL-17A\u7b49\u7ec6\u80de\u56e0\u5b50\u7684\u8868\u8fbe\u60c5\u51b5\u3002<\/p>\n\n\n\n

Question & Answer<\/strong><\/h2>\n\n\n\n

Q \u7834\u819c\u5242\u6709\u6c89\u6dc0\u3001\u6709\u4e0d\u540c\u989c\u8272\uff0c\u662f\u6b63\u5e38\u73b0\u8c61\u5417\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u7834\u819c\u5242\u5185\u7684\u6c89\u6dc0\u901a\u5e38\u662f\u76d0\u6790\u51fa\uff0c\u4e0d\u5f71\u54cd\u4f7f\u7528\u3002\u7531\u4e8e\u989c\u8272\u4e0d\u5728\u4ea7\u54c1\u8d28\u68c0\u9879\u76ee\u5185\uff0c\u4e0d\u540c\u6279\u6b21\u53ef\u80fd\u4f1a\u51fa\u73b0\u989c\u8272\u5dee\u5f02\uff0c\u4f46\u4e0d\u5f71\u54cd\u4ea7\u54c1\u6027\u80fd\u3002<\/p>\n\n\n\n

Q \u7ec6\u80de\u6837\u672c\u7ecf\u8fc7\u8868\u9762\u67d3\u8272\u540e\u662f\u5426\u53ef\u4ee5\u4fdd\u5b58\u5728IC\u56fa\u5b9a\u6db2\u91cc\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u67d3\u8272\u5b8c\u6210\u540e\u6211\u4eec\u5efa\u8bae\u5c3d\u5feb\u4e0a\u673a\u68c0\u6d4b\uff0c\u5982\u679c\u5b9e\u5728\u6765\u4e0d\u53ca\u4e0a\u673a\uff0c\u53ef\u4ee5\u5c06100 \u03bcL \u6837\u54c1\u4e0e100 \u03bcL IC\u56fa\u5b9a\u6db2\uff08\u8d27\u53f700-8222-49\uff09\u6df7\u5408\uff0c\u4ece\u800c\u5c06\u6837\u54c1\u4fdd\u5b58\u5728IC\u56fa\u5b9a\u6db2\u4e2d\u3002<\/p>\n\n\n\n

Q \u80fd\u5426\u5148\u5bf9\u7ec6\u80de\u8fdb\u884c\u56fa\u5b9a\u7834\u819c\uff0c\u7136\u540e\u518d\u5bf9\u8868\u9762\u548c\u80de\u5185\u6807\u5fd7\u7269\u67d3\u8272\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u7406\u8bba\u4e0a\u6765\u8bf4\u53ef\u4ee5\uff0c\u4f46\u662f\u9700\u8981\u6ce8\u610f\u6297\u4f53\u7684\u514b\u9686\u53f7\u662f\u5426\u8010\u53d7\u56fa\u5b9a\/\u7834\u819c\u5904\u7406\uff0c\u8d5b\u9ed8\u98de\u63a2\u7d22\u4e86\u90e8\u5206\u6297\u4f53\u4e0d\u540c\u514b\u9686\u53f7\u7684\u517c\u5bb9\u6027\uff0c\u70b9\u51fb\u4e86\u89e3\u66f4\u591a\u4fe1\u606f<\/a>\u3002<\/p>\n\n\n\n

Q \u4e3a\u4ec0\u4e48\u56fa\u5b9a\u7834\u819c\u540e\uff0c\u8868\u9762\u6807\u5fd7\u7269\u7684\u8367\u5149\u4e22\u5931\u4e86\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u5982\u679c\u6297\u4f53\u53d1\u751f\u5185\u5316\u7684\u8bdd\uff0c\u53ef\u80fd\u65e0\u6cd5\u68c0\u6d4b\u5230\u8868\u9762\u7684\u6807\u5fd7\u7269\uff1b\u6b64\u5916\u5982\u679c\u56fa\u5b9a\u7834\u819c\u8bd5\u5242\u4e2d\u5982\u679c\u542b\u9187\u7c7b\u8bd5\u5242\uff0888-8824-00\u5185\u7684\u56fa\u5b9a\u7834\u819c\u5242\u662f\u4e0d\u542b\u9187\u7c7b\u8bd5\u5242\u7684\uff09<\/strong>\uff0c\u4f1a\u6709\u5bfc\u81f4\u8367\u5149\u6dec\u706d\u7684\u53ef\u80fd\uff0c\u5c24\u5176\u5bf9\u4e8e\u8367\u5149\u86cb\u767d\u7c7b\u67d3\u6599\u5f71\u54cd\u6700\u5927\u3002\u56e0\u6b64\uff0c\u8981\u4f7f\u7528\u542b\u9187\u7c7b\u8bd5\u5242\u7684\u56fa\u5b9a\u7834\u819c\u5242\uff0c\u8868\u9762\u67d3\u8272\u5c3d\u91cf\u907f\u514d\u4f7f\u7528APC\u3001PE\u548cPerCP\u53ca\u5176\u590d\u5408\u67d3\u6599\u5076\u8054\u7684\u6297\u4f53\u3002\u7834\u819c\u64cd\u4f5c\u540e\uff0c\u53ef\u4ee5\u7ee7\u7eed\u4f7f\u7528\u542b\u6709\u8367\u5149\u86cb\u767d\u7c7b\u5076\u67d3\u6599\u8054\u7684\u6297\u4f53\u8fdb\u884c\u80de\u5185\u6807\u8bb0\u3002<\/p>\n\n\n\n

*\u4ee5\u4e0a\u6570\u636e\u5747\u51fa\u81ea\u8d5b\u9ed8\u98de\u6d41\u5f0f\u7ec6\u80de\u672f\u7cbe\u54c1\u57f9\u8bad\u73ed\uff0c\u6240\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e3aAttune\u2122 NxT\u6d41\u5f0f\u7ec6\u80de\u4eea\u3002<\/p>\n\n\n\n

\"contact<\/a><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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