{"id":1170,"date":"2026-03-25T06:51:31","date_gmt":"2026-03-25T06:51:31","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1170"},"modified":"2026-04-22T21:59:27","modified_gmt":"2026-04-22T21:59:27","slug":"isotype-control","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/isotype-control\/","title":{"rendered":"\u6d41\u5f0f\u540c\u578b\u5bf9\u7167 (Isotype Control)"},"content":{"rendered":"\n

\u65b0\u5b66\u671f\u5f00\u5b66\u540e\uff0c\u540c\u5b66\u4eec\u4ecd\u7136\u8fd8\u662f\u5728\u6d41\u5f0f\u5bf9\u7167\u4e0a\u6709\u4e9b\u95ee\u9898\uff0c\u8ba9\u6211\u4eec\u4e00\u8d77\u53bb\u95ee\u95eeDr.\u8d5b\u5427\u3002<\/strong><\/p>\n\n\n\n


Q Dr.\u8d5b\uff0c\u6211\u53d1\u73b0eBioscience\u6d41\u5f0f\u6297\u4f53\u5728\u5b98\u7f51\u4e0a\u4f1a\u63a8\u8350\u540c\u578b\u5bf9\u7167\u6297\u4f53\uff0c\u540c\u578b\u5bf9\u7167\u6297\u4f53\u662f\u5565\u610f\u601d\u554a\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u540c\u578b\u5bf9\u7167\u6297\u4f53\u662f\u6307\u4e0e\u76ee\u7684\u6297\u4f53\u79cd\u5c5e\u6765\u6e90\u548c\u4e9a\u578b\u76f8\u540c\uff0c\u5e26\u6709\u76f8\u540c\u8367\u5149\u6807\u8bb0\uff0c\u4f46\u4e0d\u80fd\u4e0e\u9776\u6807\u4ea7\u751f\u7279\u5f02\u6027\u7ed3\u5408\u7684\u6297\u4f53\u3002<\/p>\n\n\n\n

Q \u90a3\u2026\u2026\u4e3a\u4ec0\u4e48\u8981\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167\u5462\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u76ee\u7684\u662f\u6392\u9664\u6297\u4f53\u7684\u975e\u7279\u5f02\u6027\u7ed3\u5408\u5bf9\u5b9e\u9a8c\u4ea7\u751f\u5e72\u6270\u3002\u8ba9\u6211\u4eec\u4eca\u5929\u4e00\u8d77\u4e86\u89e3\u4e0b\u540c\u578b\u5bf9\u7167\u5427\uff01<\/p>\n\n\n\n

\u4ec0\u4e48\u662f\u540c\u578b\u5bf9\u7167\uff0c\u5982\u4f55\u9009\u62e9\u540c\u578b\u5bf9\u7167\u6297\u4f53<\/strong><\/h2>\n\n\n\n

\u60f3\u5fc5\u5927\u5bb6\u90fd\u6709\u5728\u6d41\u5f0f\u68c0\u6d4b\u4e2d\u9047\u5230\u67d0\u4e2a\u7ec6\u80de\u5206\u7fa4\u4e0d\u660e\u663e\u7684\u60c5\u51b5\uff0c\u8fd9\u4f1a\u4f7f\u6211\u4eec\u5f88\u96be\u5206\u6790\u5212\u95e8\u3002\u6211\u4eec\u53ea\u5e0c\u671b\u76ee\u7684\u6297\u4f53\u80fd\u591f\u7279\u5f02\u6027\u5730\u7ed3\u5408\u76f8\u5e94\u9776\u6807\uff0c\u4f46\u4e8b\u5b9e\u5e76\u975e\u5982\u6b64\uff0c\u9664\u5f00\u6297\u4f53Fab\u7aef\u6297\u539f\u8bc6\u522b\u4f4d\u70b9\u5916\uff0c\u76ee\u7684\u6297\u4f53\u5176\u4ed6\u90e8\u5206\u4e5f\u53ef\u80fd\u56e0\u4e3a\u5404\u79cd\u4f5c\u7528\u529b\u8ddf\u9776\u6807\u7ed3\u5408\uff0c\u5373\u975e\u7279\u5f02\u6027\u7ed3\u5408\u3002\u5982\u679c\u6709\u8fd9\u6837\u7684\u6297\u4f53\u2014\u2014\u9664\u4e86\u6297\u539f\u8bc6\u522b\u4f4d\u70b9\u4ee5\u5916\u8ddf\u76ee\u7684\u6297\u4f53\u51e0\u4e4e\u4e00\u6a21\u4e00\u6837\uff08\u5982\u88681\u793a\u4f8b\uff09\uff0c\u6211\u4eec\u53ef\u4ee5\u7528\u5b83\u6765\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u6392\u9664\u975e\u7279\u5f02\u6027\u7ed3\u5408\u5bf9\u5b9e\u9a8c\u7684\u5e72\u6270\uff0c\u5e2e\u52a9\u6211\u4eec\u5212\u95e8\u3002\u8fd9\u5c31\u662f\u540c\u578b\u5bf9\u7167\u3002<\/strong><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u88681. \u540c\u578b\u5bf9\u7167\u3001\u76ee\u7684\u6297\u4f53\u5bf9\u6bd4\u793a\u4f8b<\/p>\n\n\n\n

\u6211\u4eec\u6765\u770b\u770b\u4f7f\u7528\u4e0a\u8868\u4e2d\u7684\u76ee\u7684\u6297\u4f53\u548c\u540c\u578b\u5bf9\u7167\u6297\u4f53\u68c0\u6d4bMouse Foxp3\u7684\u6570\u636e\uff08\u56fe1\uff09\uff1a\u5bbf\u4e3b\/\u4e9a\u578b\u540c\u6837\u4e3aRat \/ IgG2a, kappa\u4e14\u8367\u5149\u4e3aPE\u7684\u6297\u4f53\u6807\u8bb0\u6837\u54c1\u4f5c\u4e3a\u540c\u578b\u5bf9\u7167\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5212\u51fa\u9634\u6027\u7ec6\u80de\u7fa4\u3002\u5728\u4e86\u89e3\u540c\u578b\u5bf9\u7167\u7684\u542b\u4e49\u53ca\u4f5c\u7528\u4e4b\u540e\uff0c\u76f8\u4fe1\u5927\u5bb6\u5df2\u7ecf\u77e5\u9053\u9009\u62e9\u540c\u578b\u5bf9\u7167\u7684\u539f\u5219\u4e86\uff1a1\uff09\u79cd\u5c5e\u6765\u6e90<\/strong>\u76f8\u540c\uff0c2\uff09\u4e9a\u578b<\/strong>\u76f8\u540c\uff0c3\uff09\u8367\u5149\u6807\u8bb0<\/strong>\u76f8\u540c\uff0c4\uff09\u4e0d\u80fd<\/strong>\u8ddf\u7279\u5f02\u6027\u9776\u6807\u7ed3\u5408\u3002PS\uff1a\u6211\u4eec\u7684\u6bcf\u652feBioscience\u6d41\u5f0f\u6297\u4f53\u5b98\u7f51\u9875\u9762\u6709\u76f8\u5e94\u540c\u578b\u5bf9\u7167\u63a8\u8350\uff0c\u5f88\u65b9\u4fbf\u5594\uff01<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe1. \u540c\u578b\u5bf9\u7167\u6297\u4f53\u6392\u9664\u975e\u7279\u5f02\u6027\u7ed3\u5408\u4ea7\u751f\u7684\u80cc\u666f\u8367\u5149\u3002\u4f7f\u7528\u540c\u578b\u5bf9\u7167\u6297\u4f53\uff08Rat IgG2a PE\uff09\u6216Foxp3 PE\u6297\u4f53\u5bf9\u5c0f\u9f20\u813e\u810f\u5355\u7ec6\u80de\u60ac\u6db2\u8fdb\u884c\u67d3\u8272\u5e76\u8fdb\u884c\u6d41\u5f0f\u5206\u6790\u3002<\/p>\n\n\n\n

\u4ec0\u4e48\u60c5\u51b5\u4e0b\u9700\u8981\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167<\/strong><\/h2>\n\n\n\n

\u5bf9\u4e8e\u4e00\u4e9b\u8fde\u7eed\u8868\u8fbe\u3001\u4f4e\u8868\u8fbe\u6216\u6fc0\u6d3b\u6027\u9776\u6807<\/strong>\uff08\u5982CD45RO\u3001CD25\u3001CD80\u7b49\uff09\uff0c\u901a\u5e38\u6ca1\u6709\u660e\u663e\u9634\u9633\u6027\u5206\u7fa4\uff0c\u8fd9\u65f6\u53ef\u4ee5\u8003\u8651\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167\uff0c\u5e2e\u52a9\u5212\u5206\u9633\u6027\u7ec6\u80de\u7fa4\u3002\u6211\u4eec\u53ef\u4ee5\u770b\u770b\u56fe2a\u8fd9\u4e2a\u6848\u4f8b\uff0c\u4f7f\u7528CD25 Monoclonal Antibody (CD25-4E3), APC, eBioscience\u2122\uff08\u8d27\u53f717-0257-42\uff09\u68c0\u6d4bHuman CD25\uff0c\u6ca1\u6709\u660e\u663e\u7684\u5206\u7fa4\u754c\u9650\uff0c\u53ef\u4ee5\u4f7f\u7528\u540c\u578b\u5bf9\u7167\u6297\u4f53Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience\u2122\uff08\u8d27\u53f717-4732-81\uff09\u6807\u8bb0\u6837\u54c1\u4e4b\u540e\u8f85\u52a9\u5212\u95e8\u3002<\/p>\n\n\n\n

\u5bf9\u4e8e\u9ad8\u8868\u8fbe\u9776\u6807<\/strong>\uff08\u5982CD45\u3001CD3\u3001CD4\u7b49\uff09\uff0c\u5728\u7535\u538b\u8bbe\u7f6e\u5408\u9002\u7684\u60c5\u51b5\u4e0b\u901a\u5e38\u5206\u7fa4\u660e\u663e\uff0c\u4e00\u822c\u4e0d\u9700\u8981\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167\u3002\u6211\u4eec\u53ef\u4ee5\u770b\u770b\u56fe2b\u8fd9\u4e2a\u6848\u4f8b\uff0cCD45\uff08\u6d0b\u7ea2\u8272\u5cf0\uff09\u4fe1\u53f7\u5f3a\u4e14\u5206\u7fa4\u660e\u663e\uff0c\u5373\u4f7f\u4e0d\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167\uff08\u84dd\u8272\u5cf0\uff09\u4e5f\u80fd\u8f7b\u677e\u5212\u95e8\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe2a. CD25 APC\u53ca\u540c\u578b\u5bf9\u7167Mouse IgG2b APC <\/p>\n\n\n\n

b.CD45 PE\uff08\u6d0b\u7ea2\u8272\u76f4\u65b9\u56fe\uff09\u53ca\u540c\u578b\u5bf9\u7167\uff08\u84dd\u8272\u76f4\u65b9\u56fe\uff09<\/p>\n\n\n\n

\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u867d\u7136\u540c\u578b\u5bf9\u7167\u80fd\u591f\u5e2e\u52a9\u6211\u4eec\u5212\u95e8\uff0c\u4f46\u5b83\u5e76\u4e0d\u80fd\u76f4\u63a5\u4f5c\u4e3a\u5212\u95e8\u7684\u4f9d\u636e\uff0c<\/strong>\u6765\u770b\u770b\u4e0b\u9762\u8fd9\u4e2a\u6848\u4f8b\u5427\u3002\u5b9e\u9a8c\u7ec4\u6709\u8f83\u4e3a\u660e\u663e\u7684\u5206\u7fa4\uff0c\u4f46\u5982\u679c\u76f4\u63a5\u4ee5\u540c\u578b\u5bf9\u7167\u6563\u70b9\u56fe\u5206\u5e03\u6765\u5212\u95e8\uff0c\u5c31\u4f1a\u201c\u9519\u5931\u201d\u5f88\u591a\u9633\u6027\u7ec6\u80de\uff1b\u8fd9\u65f6\u4ee5\u5b9e\u9a8c\u7ec4\u5206\u7fa4\u7ed3\u679c\u6765\u5212\u95e8\uff0c\u53ef\u4ee5\u770b\u5230\u540c\u578b\u5bf9\u7167\u7ec4\u548c\u7a7a\u767d\u5bf9\u7167\u7ec4P2\u533a\u57df\u7ec6\u80de\u5360\u6bd4\u5747\u4e3a0.9%\uff0c\u6700\u540e\u5c06\u5b9e\u9a8c\u7ec4\u9633\u6027\u7387\u51cf\u53bb0.9%\u5373\u53ef\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe3  MDA-MB-231\u7ec6\u80de\u7a7a\u767d\u5bf9\u7167\uff08\u5de6\uff09\u3001\u540c\u578b\u5bf9\u7167\uff08\u4e2d\uff09\u548c\u5b9e\u9a8c\u7ec4\uff08\u53f3\uff09<\/p>\n\n\n\n

\u5bf9\u4e8e\u540c\u578b\u5bf9\u7167\u7684\u4f7f\u7528\u7684\u601d\u8003<\/strong><\/h2>\n\n\n\n

\u54f2\u5b66\u5bb6\u66fe\u8bf4\uff0c\u4e16\u754c\u4e0a\u6ca1\u6709\u5b8c\u5168\u76f8\u540c\u7684\u4e24\u7247\u6811\u53f6\u3002\u90a3\u4e48Dr.\u8d5b\u8bf4\uff0c\u4e16\u754c\u4e0a\u4e5f\u6ca1\u6709\u5b8c\u5168\u76f8\u540c\u7684\u4e24\u53ea\u5c0f\u9f20\u3002\u65e2\u7136\u76ee\u7684\u6297\u4f53\u548c\u540c\u578b\u5bf9\u7167\u6297\u4f53\u6765\u81ea\u4e0d\u540c\u5bbf\u4e3b\uff0c\u90a3\u4e48\u4e5f\u5c31\u4e0d\u5b58\u5728\u5b8c\u7f8e\u7684\u201c\u9664\u6297\u539f\u8bc6\u522b\u4f4d\u70b9\u5916\u5168\u90fd\u76f8\u540c\u201d\u7684\u8bf4\u6cd5\u3002\u8fd9\u4e5f\u662f\u6587\u4e2dDr.\u8d5b\u5728\u8bb2\u89e3\u540c\u578b\u5bf9\u7167\u65f6\u7528\u8bcd<\/strong>\uff08\u53ef\u80fd\u3001\u51e0\u4e4e\u3001\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u2026\u2026\uff09\u975e\u5e38\u514b\u5236<\/strong>\u7684\u539f\u56e0\u3002<\/p>\n\n\n\n

\u90a3\u4e48\u540c\u578b\u5bf9\u7167\u5230\u5e95\u6709\u6ca1\u6709\u8bbe\u7f6e\u7684\u5fc5\u8981\u5462\uff1f<\/strong>\u4ece\u5b9e\u9a8c\u7684\u89d2\u5ea6\u6765\u8bf4\uff0c\u9700\u8981\u6839\u636e\u81ea\u5df1\u7684\u5e94\u7528\u53bb\u51b3\u5b9a\u662f\u5426\u4f7f\u7528\u540c\u578b\u5bf9\u7167\uff1a\u5728\u591a\u8272panel\u5206\u6790\u65f6\u540c\u578b\u5bf9\u7167\u9700\u8981\u8ddfFMO(\u8367\u5149\u51cf\u4e00)\u5bf9\u7167\u8054\u5408\u4f7f\u7528\uff1b\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u7684\u67d0\u4e9b\u6b65\u9aa4\uff08\u5982\u56fa\u5b9a\u7834\u819c\uff0c\u591a\u514b\u9686\u6297\u4f53\u4f7f\u7528\u2026\u2026\uff09\u53ef\u80fd\u5bfc\u81f4\u8f83\u591a\u975e\u7279\u5f02\u6027\u7ed3\u5408\u65f6\uff0c\u4e5f\u5efa\u8bae\u8bbe\u7f6e\u540c\u578b\u5bf9\u7167\uff1b\u5f53\u6211\u4eec\u53bb\u63a2\u7d22\u4e00\u4e2a\u65b0\u7684\u9776\u6807\uff0c\u4e0d\u6e05\u695a\u5176\u8868\u8fbe\u6c34\u5e73\uff0c\u540c\u578b\u5bf9\u7167\u80fd\u591f\u4f5c\u4e3a\u6211\u4eec\u5212\u95e8\u7684\u91cd\u8981\u53c2\u8003\u3002Maurice R.G. O\u2019Gorman\u5728Isotype Controls\u2014Time to Let Go? \u4e00\u6587\u4e2d\u5bf9\u540c\u578b\u5bf9\u7167\u7684\u8bbe\u7f6e\u8868\u8fbe\u4e86\u80af\u5b9a\uff1aSaying goodbye to isotype controls is premature. The use of isotype specific controls should be left up to the discretion of the individual responsible for the laboratory. \u867d\u7136\u8fd9\u7bc7\u6587\u732e\u6bd4\u8f83\u4e45\u8fdc\uff0c\u4f46\u5176\u4e2d\u89c2\u70b9\u4f9d\u7136\u5f88\u6709\u4ef7\u503c\uff0c\u611f\u5174\u8da3\u7684\u8bdd\u53ef\u4ee5\u53bb\u641c\u641c\u539f\u6587\u3002<\/p>\n\n\n\n

Question & Answer<\/strong><\/h2>\n\n\n\n

Q \u540c\u578b\u5bf9\u7167\u7684\u7528\u91cf\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u7528\u91cf\u8ddf\u76ee\u7684\u6297\u4f53\u4e00\u81f4\uff1b\u6bd4\u5982\u76ee\u7684\u6297\u4f53\u52a00.25 \u03bcg\uff0c\u540c\u578b\u5bf9\u7167\u6297\u4f53\u4e5f\u52a00.25 \u03bcg\u3002<\/p>\n\n\n\n

Q \u5982\u82e5\u7f51\u9875\u4e0a\u6ca1\u6709\u63a8\u8350\u540c\u578b\u5bf9\u7167\u7684\u6297\u4f53\uff0c\u5982\u4f55\u67e5\u627e\u5176\u540c\u578b\u5bf9\u7167\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u5728\u6d4f\u89c8\u5668\u4e2d\u68c0\u7d22\u8be5\u7f51\u5740<\/a>\u6839\u636e\u7b5b\u9009\u5668Filter\u8fdb\u884c\u7b5b\u9009\u3002<\/p>\n\n\n\n

*\u4ee5\u4e0a\u6570\u636e\u5747\u51fa\u81ea\u8d5b\u9ed8\u98de\u6d41\u5f0f\u7ec6\u80de\u672f\u7cbe\u54c1\u57f9\u8bad\u73ed\uff0c\u6240\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e3aAttune\u2122 NxT\u6d41\u5f0f\u7ec6\u80de\u4eea\u3002<\/p>\n\n\n\n

\"contact<\/a><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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