{"id":1237,"date":"2026-03-26T02:43:01","date_gmt":"2026-03-26T02:43:01","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1237"},"modified":"2026-04-22T21:59:26","modified_gmt":"2026-04-22T21:59:26","slug":"guide-to-selecting-immunofluorescence-methods","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/guide-to-selecting-immunofluorescence-methods\/","title":{"rendered":"\u514d\u75ab\u8367\u5149\u65b9\u6cd5\u9009\u62e9\u6307\u5357\uff1a\u4ece\u5165\u95e8\u5230\u8fdb\u9636\uff0c\u4e00\u6587\u8bb2\u900f\uff01"},"content":{"rendered":"\n<p>\u5728\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4e2d\uff0c\u514d\u75ab\u8367\u5149\uff08Immunofluorescence, IF\uff09\u662f\u6700\u5e38\u7528\u7684\u6210\u50cf\u624b\u6bb5\u4e4b\u4e00\u3002\u5b83\u80fd\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u5728\u7ec6\u80de\u4e0e\u7ec4\u7ec7\u4e2d\u201c\u770b\u5230\u201d\u86cb\u767d\u8d28\u7684\u7a7a\u95f4\u5206\u5e03\u3001\u8868\u8fbe\u53d8\u5316\u3001\u4fe1\u53f7\u901a\u8def\u4fe1\u606f\uff0c\u751a\u81f3\u662f\u75be\u75c5\u5fae\u73af\u5883\u4e2d\u7684\u590d\u6742\u7ec6\u80de\u5173\u7cfb\u3002<\/p>\n\n\n\n<p>\u7136\u800c\uff0c\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\uff0c<strong>\u514d\u75ab\u8367\u5149\u7684\u9009\u62e9\u5df2\u7ecf\u4e0d\u518d\u662f\u7b80\u5355\u7684\u201c\u7528\u4ec0\u4e48\u989c\u8272\u201d\u8fd9\u4e48\u5bb9\u6613\u3002<\/strong>\u9762\u5bf9<strong>\u9ad8\u4f4e\u4e0d\u540c\u7684\u9776\u6807\u4e30\u5ea6<\/strong>\u3001\u4e0d\u65ad\u589e\u957f\u7684<strong>\u591a\u91cd\u68c0\u6d4b<\/strong>\u9700\u6c42\u3001\u5404\u5f0f\u5404\u6837\u7684\u4fe1\u53f7\u653e\u5927\u65b9\u5f0f\u4e0e\u6210\u50cf\u5e73\u53f0\uff0c\u5982\u4f55\u9009\u62e9\u6700\u5408\u9002\u7684\u514d\u75ab\u8367\u5149\u7b56\u7565\uff0c\u6210\u4e3a\u8bb8\u591a\u79d1\u7814\u4eba\u5458\u7684\u5171\u540c\u56f0\u60d1\u3002<\/p>\n\n\n\n<p>\u4eca\u5929\uff0c\u8fd9\u7bc7\u63a8\u6587\u5c06\u5e26\u4f60\u4ece\u5e94\u7528\u573a\u666f\u5230\u524d\u6cbf\u6280\u672f\uff0c\u518d\u5230\u65b9\u6cd5\u9009\u62e9\u7684\u6838\u5fc3\u56e0\u7d20\uff0c\u6784\u5efa\u4e00\u5957\u6e05\u6670\u3001\u5b9e\u7528\u7684<strong>\u514d\u75ab\u8367\u5149\u65b9\u6cd5\u9009\u62e9\u6307\u5357<\/strong>\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u514d\u75ab\u8367\u5149\u7684\u5e38\u89c1\u5e94\u7528\u573a\u666f\"><strong>\u514d\u75ab\u8367\u5149\u7684\u5e38\u89c1\u5e94\u7528\u573a\u666f<\/strong><\/h2>\n\n\n\n<p>\u514d\u75ab\u8367\u5149\u51e0\u4e4e\u51fa\u73b0\u5728\u6240\u6709\u4e0e\u7ec6\u80de\u3001\u7ec4\u7ec7\u76f8\u5173\u7684\u7814\u7a76\u9886\u57df\uff0c\u5305\u62ec\uff1a<\/p>\n\n\n\n<p><strong>\u7ec6\u80de\u751f\u7269\u5b66\u7814\u7a76\uff1a\u63a2\u7a76\u86cb\u767d\u5b9a\u4f4d\u4e0e\u52a8\u6001\u53d8\u5316<\/strong><br>\u4f8b\u5982\u89c2\u5bdf\u7ec6\u80de\u9aa8\u67b6\u3001\u8f6c\u5f55\u56e0\u5b50\u5b9a\u4f4d\u3001\u7ec6\u80de\u5468\u671f\u86cb\u767d\u7b49\u3002<\/p>\n\n\n\n<p><strong>\u80bf\u7624\u5fae\u73af\u5883\u89e3\u6790<\/strong><br>\u591a\u91cd\u514d\u75ab\u8367\u5149\u80fd\u540c\u65f6\u8bc6\u522b\u514d\u75ab\u7ec6\u80de\u4e9a\u7fa4\u3001\u80bf\u7624\u6807\u5fd7\u7269\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\u3001\u8840\u7ba1\u751f\u6210\u7b49\u5173\u952e\u7279\u5f81\u3002<\/p>\n\n\n\n<p><strong>\u795e\u7ecf\u79d1\u5b66\u7814<\/strong><br>\u7528\u4e8e\u8bc6\u522b\u795e\u7ecf\u5143\u4e9a\u578b\u3001\u795e\u7ecf\u80f6\u8d28\u7ec6\u80de\u3001\u7a81\u89e6\u6807\u5fd7\u7269\u53ca\u75c5\u7406\u6c89\u79ef\u3002<\/p>\n\n\n\n<p><strong>\u7ec4\u7ec7\u5b66\u4e0e\u75c5\u7406\u7814\u7a76<\/strong><br>\u5e2e\u52a9\u75c5\u7406\u7814\u7a76\u8005\u89c2\u5bdf\u7ec6\u80de\u5171\u8868\u8fbe\u3001\u533a\u57df\u7279\u5f02\u6027\u53d8\u5316\u3001\u75be\u75c5\u6a21\u578b\u4e2d\u7684\u7ec4\u7ec7\u7ed3\u6784\u53d8\u5316\u3002<\/p>\n\n\n\n<p><strong>\u836f\u6548 &amp; \u6bd2\u7406\u5b66\u8bc4\u4f30<\/strong><br>\u5b9a\u4f4d\u836f\u7269\u9776\u70b9\u3001\u68c0\u6d4b\u751f\u7269\u6807\u5fd7\u7269\u53d8\u5316\uff0c\u662f\u65b0\u836f\u7814\u53d1\u7684\u91cd\u8981\u624b\u6bb5\u3002<\/p>\n\n\n\n<p>\u514d\u75ab\u8367\u5149\u7684\u4ef7\u503c\u5728\u4e8e\uff1a<strong>\u4e0d\u4ec5\u770b\u201c\u6709\u6ca1\u6709\u201d\uff0c\u8fd8\u80fd\u770b\u201c\u5728\u54ea\u91cc\u201d\u3002<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u514d\u75ab\u8367\u5149\u7684\u524d\u6cbf\u6280\u672f-\u4ece\u4f20\u7edf\u4e09\u8272\u5230\u9ad8\u7ef4\u7a7a\u95f4\u89e3\u6790\"><strong>\u514d\u75ab\u8367\u5149\u7684\u524d\u6cbf\u6280\u672f\uff1a\u4ece\u4f20\u7edf\u4e09\u8272\u5230\u9ad8\u7ef4\u7a7a\u95f4\u89e3\u6790<\/strong><\/h2>\n\n\n\n<p>\u968f\u7740\u7a7a\u95f4\u7ec4\u5b66\u7684\u5174\u8d77\uff0c\u514d\u75ab\u8367\u5149\u65e9\u5df2\u4ece\u4f20\u7edf\u7684 3\u20134 \u8272\u6210\u50cf\u53d1\u5c55\u4e3a\u80fd\u4e00\u6b21\u6027\u83b7\u53d6\u5927\u91cf\u4fe1\u606f\u7684\u524d\u6cbf\u6280\u672f\uff1a<\/p>\n\n\n\n<p><strong>\u5149\u8c31\u6210\u50cf + \u5149\u8c31\u62c6\u5206<\/strong><br>\u53ef\u5c06 8\u201310 \u79cd\u5149\u8c31\u91cd\u53e0\u7684\u67d3\u6599\u533a\u5206\u5f00\uff0c\u5b9e\u73b0<strong>\u5355\u6b21\u626b\u63cf\u591a\u91cd\u6210\u50cf<\/strong>\uff0c\u5e38\u7528\u4e8e\u4e2d\u901a\u91cf\u7a7a\u95f4\u7ec4\u5b66\u5206\u6790\u3002<\/p>\n\n\n\n<p><strong>\u916a\u80fa\u4fe1\u53f7\u653e\u5927<\/strong><br>\u9002\u7528\u4e8e\u4f4e\u4e30\u5ea6\u9776\u6807\uff0c\u80fd\u663e\u8457\u63d0\u5347\u4fe1\u566a\u6bd4\uff0c\u5c24\u5176\u9002\u5408 FFPE \u7ec4\u7ec7\u548c\u5f31\u8868\u8fbe\u86cb\u767d\u3002<\/p>\n\n\n\n<p><strong>\u5faa\u73af\u514d\u75ab\u8367\u5149<\/strong><br>\u901a\u8fc7\u201c\u67d3\u8272\u2014\u6210\u50cf\u2014\u5265\u79bb\u2014\u518d\u67d3\u8272\u201d\uff0c\u53ef\u68c0\u6d4b 20\u201360+ \u6807\u8bb0\u7269\uff0c\u662f\u9ad8\u901a\u91cf\u7a7a\u95f4\u7ec4\u5b66\u7684\u91cd\u8981\u624b\u6bb5\u3002<\/p>\n\n\n\n<p><strong>DNA \u6761\u5f62\u7801\u6280\u672f<\/strong><br>\u5927\u5e45\u6269\u5c55\u68c0\u6d4b\u7ef4\u5ea6\uff0c\u662f\u672a\u6765\u9ad8\u901a\u91cf\u7a7a\u95f4\u86cb\u767d\u7ec4\u5b66\u7684\u6838\u5fc3\u5de5\u5177\u4e4b\u4e00\u3002<\/p>\n\n\n\n<p>\u8fd9\u4e9b\u524d\u6cbf\u6280\u672f\u7684\u5171\u540c\u76ee\u6807\u662f\uff1a<br><strong>\u7528\u66f4\u5c11\u7684\u7ec4\u7ec7\uff0c\u5f97\u5230\u66f4\u6df1\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u514d\u75ab\u8367\u5149\u65b9\u6cd5\u9009\u62e9\u7684\u5173\u952e\u8003\u91cf\u56e0\u7d20\"><strong>\u514d\u75ab\u8367\u5149\u65b9\u6cd5\u9009\u62e9\u7684\u5173\u952e\u8003\u91cf\u56e0\u7d20<\/strong><\/h2>\n\n\n\n<p>\u5728\u9009\u62e9\u514d\u75ab\u8367\u5149\u7b56\u7565\u65f6\uff0c\u53ef\u4ee5\u4ece\u4ee5\u4e0b 10 \u5927\u6838\u5fc3\u56e0\u7d20\u51fa\u53d1\uff1a<\/p>\n\n\n\n<p><strong>1.\u9776\u6807\u4e30\u5ea6\uff1a\u51b3\u5b9a\u662f\u5426\u9700\u8981\u653e\u5927<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u9ad8\u4e30\u5ea6\uff1a\u76f4\u63a5\u6297\u4f53\u5373\u53ef<\/li>\n\n\n\n<li>\u4e2d\u4e30\u5ea6\uff1a\u4e8c\u6297\u653e\u5927\u6216\u9ad8\u4eae\u5ea6\u8367\u5149\u67d3\u6599<\/li>\n\n\n\n<li>\u4f4e\u4e30\u5ea6\uff1a\u9176\u4fc3\u653e\u5927\u6548\u679c\u6700\u4f73\uff08\u5982\u916a\u80fa\u4fe1\u53f7\u653e\u5927\uff09<\/li>\n<\/ul>\n\n\n\n<p><strong>2.\u591a\u91cd\u68c0\u6d4b\u9700\u6c42<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3\u20134 \u8272 \u2192 \u5e38\u89c4 IF<\/li>\n\n\n\n<li>5\u201310 \u8272 \u2192 \u5149\u8c31\u6210\u50cf<\/li>\n\n\n\n<li>10\u201360 \u8272 \u2192 \u5faa\u73af\u6210\u50cf \/ DNA \u6761\u5f62\u7801<\/li>\n<\/ul>\n\n\n\n<p><strong>3.\u4fe1\u566a\u6bd4\u662f\u5426\u591f\u9ad8\uff1f<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u80cc\u666f\u9ad8 \u2192 \u539f\u56e0\uff1a\u4fe1\u53f7\u653e\u5927\u65b9\u6cd5\u4e0d\u5f53\u6216\u5149\u8c31\u62c6\u5206\u95ee\u9898<\/li>\n\n\n\n<li>\u81ea\u53d1\u8367\u5149\u5f3a\u7684\u7ec4\u7ec7\u66f4\u9700\u8981\u4f18\u5316<\/li>\n<\/ul>\n\n\n\n<p><strong>4.\u662f\u5426\u6709\u7ecf\u8fc7\u9a8c\u8bc1\u7684\u6297\u4f53\uff1f<\/strong><\/p>\n\n\n\n<p>\u9009\u62e9\u76f4\u63a5\u6807\u8bb0\u3001\u4e8c\u6297\u653e\u5927\u6216DIY\u76f4\u6807\u6297\u4f53\u9700\u6839\u636e\u662f\u5426\u6709\u53ef\u7528\u6297\u4f53\u548c\u6297\u4f53\u7684\u5e94\u7528\u9a8c\u8bc1\u6570\u636e\u51b3\u5b9a\u3002<\/p>\n\n\n\n<p><strong>5.\u7ec4\u7ec7\u7c7b\u578b\u4e0e\u6837\u54c1\u8d28\u91cf<\/strong><\/p>\n\n\n\n<p>FFPE \u81ea\u53d1\u8367\u5149\u9ad8\u3001\u539a\u7ec4\u7ec7\u5149\u6563\u5c04\u5f3a\u3001\u77ff\u5316\u7ec4\u7ec7\u96be\u5904\u7406 \u2192 \u4fe1\u53f7\u653e\u5927\u65b9\u6cd5\u9700\u6700\u5927\u5316\u4fe1\u566a\u6bd4\u3002<\/p>\n\n\n\n<p><strong>6.\u6210\u50cf\u5e73\u53f0\u80fd\u529b<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u662f\u5426\u652f\u6301\u5149\u8c31\u6210\u50cf\uff1f<\/li>\n\n\n\n<li>\u76f8\u673a\u7075\u654f\u5ea6\u5982\u4f55\uff1f<\/li>\n\n\n\n<li>\u80fd\u5426\u8fdb\u884c\u5faa\u73af\u6210\u50cf\uff1f<\/li>\n<\/ul>\n\n\n\n<p><strong>7.\u5b9e\u9a8c\u6d41\u7a0b\u590d\u6742\u5ea6\u4e0e\u901a\u91cf<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u60f3\u5feb\uff1f\u2192 \u76f4\u63a5 IF<\/li>\n\n\n\n<li>\u8981\u5e73\u8861\u4eae\u5ea6\uff1f\u2192 \u5149\u8c31\u6210\u50cf<\/li>\n\n\n\n<li>\u8981\u6700\u4e30\u5bcc\u4fe1\u606f\uff1f\u2192 \u5faa\u73af 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id=\"h-\u5982\u4f55\u5feb\u901f\u9009\u62e9\u65b9\u6cd5\"><strong>\u5982\u4f55\u5feb\u901f\u9009\u62e9\u65b9\u6cd5\uff1f<\/strong><\/h2>\n\n\n\n<p>\ud83d\udc49 \u82e5\u9776\u6807\u5f31 \u2192 \u4f18\u5148\u9176\u4fc3\u653e\u5927\uff08Aluora\u2122\/TSA\uff09<br>\ud83d\udc49 \u82e5\u901a\u9053\u591a \u2192 \u7528\u5149\u8c31\u6210\u50cf\u6216\u5faa\u73af IF<br>\ud83d\udc49 \u82e5\u7ed3\u6784\u8106\u5f31 \u2192 \u907f\u514d\u591a\u8f6e\u5265\u79bb<br>\ud83d\udc49 \u82e5\u6837\u672c\u73cd\u8d35 \u2192 \u9009\u62e9\u5355\u6b21\u6210\u50cf\u65b9\u6848<br>\ud83d\udc49 \u82e5\u8981\u5b9a\u91cf \u2192 \u907f\u514d\u975e\u7ebf\u6027\u4fe1\u53f7\u653e\u5927\u65b9\u6cd5<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5212\u91cd\u70b9-\u9009\u62e9\u514d\u75ab\u8367\u5149\u65b9\u6cd5-\u5c31\u662f\u5e73\u8861\u827a\u672f\"><strong>\u5212\u91cd\u70b9\uff1a\u9009\u62e9\u514d\u75ab\u8367\u5149\u65b9\u6cd5\uff0c\u5c31\u662f\u5e73\u8861\u827a\u672f<\/strong><\/h2>\n\n\n\n<p>\u514d\u75ab\u8367\u5149\u6280\u672f\u6b63\u5728\u4ece\u4f20\u7edf\u89c2\u5bdf\u5de5\u5177\uff0c\u5feb\u901f\u8fc8\u5411\u7a7a\u95f4\u751f\u7269\u5b66\u7684\u6838\u5fc3\u5e73\u53f0\u3002\u9009\u62e9\u65b9\u6cd5\u4e0d\u518d\u662f\u4e00\u4e2a\u201c\u8bd5\u8bd5\u770b\u201d\u7684\u8fc7\u7a0b\uff0c\u800c\u662f\u4e00\u9879<strong>\u7efc\u5408\u9776\u6807\u7279\u6027\u3001\u7ec4\u7ec7\u6761\u4ef6\u3001\u5e73\u53f0\u80fd\u529b\u3001\u7814\u7a76\u76ee\u6807<\/strong>\u7684\u4e13\u4e1a\u5224\u65ad\u3002<\/p>\n\n\n\n<p>\u4e00\u5957\u5408\u7406\u7684\u514d\u75ab\u8367\u5149\u7b56\u7565\uff0c\u5c06\u663e\u8457\u63d0\u5347\u6210\u50cf\u8d28\u91cf\u3001\u6570\u636e\u53ef\u4fe1\u5ea6\uff0c\u5e76\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5728\u6709\u9650\u7684\u6837\u672c\u4e2d\u6d1e\u5bdf\u66f4\u591a\u751f\u7269\u5b66\u7ebf\u7d22\u3002<\/p>\n\n\n\n<p>\u5982\u679c\u4f60\u6b63\u5728\u89c4\u5212\u590d\u6742panel\u3001\u4f4e\u8868\u8fbe\u9776\u6807\u68c0\u6d4b\u6216\u7a7a\u95f4\u751f\u7269\u5b66\u5206\u6790\uff0c\u53ef\u4ee5\u53c2\u8003\u672c\u6587\u4ece<strong>\u9776\u6807\u4e30\u5ea6 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https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/03\/WX20260323-180134@2x-1-768x751.png 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/03\/WX20260323-180134@2x-1.png 1092w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><strong>Invitrogen EVOS S1000\u7a7a\u95f4\u751f\u7269\u5b66\u6210\u50cf\u5206\u6790\u7cfb\u7edf<\/strong>\u662f\u8d5b\u9ed8\u98de\u5168\u65b0\u63a8\u51fa\u7684\u4e00\u6b3e\u9ad8\u901f\u5bbd\u573a\u5149\u8c31\u6210\u50cf\u7cfb\u7edf\uff0c\u6574\u5408\u4e86\u591a\u91cd\u5149\u8c31\u8367\u5149\u3001\u900f\u5c04\u660e\u573a\u3001\u76f8\u5dee\u53ca\u5f69\u8272\u660e\u573a\u7b49\u8bf8\u591a\u6210\u50cf\u529f\u80fd\uff0c\u53ef\u5feb\u901f\u5b8c\u6210\u539f\u4f4d\u3001\u591a\u6837\u672c\u3001\u591a\u901a\u9053\u7a7a\u95f4\u7ec4\u5b66\u7814\u7a76\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" 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class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, 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