{"id":1256,"date":"2026-03-27T06:05:05","date_gmt":"2026-03-27T06:05:05","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1256"},"modified":"2026-04-22T21:59:26","modified_gmt":"2026-04-22T21:59:26","slug":"extracellular-matrix","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/extracellular-matrix\/","title":{"rendered":"\u591a\u80fd\u5e72\u7ec6\u80de\u57f9\u517b\uff1a\u7ec6\u80de\u5916\u57fa\u8d28\u600e\u4e48\u9009\uff1f\u4e00\u7bc7\u641e\u5b9a\uff01"},"content":{"rendered":"\n

PSC \u57f9\u517b\u9700\u8981\u4e00\u6574\u5957\u517c\u5bb9\u7684\u57f9\u517b\u57fa\u3001\u57fa\u8d28\u548c\u4f20\u4ee3\u65b9\u6cd5\uff0c\u4ee5\u652f\u6301\u7ec6\u80de\u7684\u5065\u5eb7\u751f\u957f\u548c\u4fdd\u6301\u591a\u80fd\u6027\u3002Thomson \u7b49\u4eba\u5728 1998 \u5e74\u9996\u6b21\u83b7\u5f97\u4eba\u80da\u80ce\u5e72\u7ec6\u80de \uff08ESCs\uff09[1]<\/sup>\uff0c\u5f53\u521d\u7684\u57f9\u517b\u4f53\u7cfb\u975e\u5e38\u590d\u6742\uff0c\u9700\u4f7f\u7528\u7ecf\u6709\u4e1d\u5206\u88c2\u706d\u6d3b\u5904\u7406\u7684\u5c0f\u9f20\u80da\u80ce\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08MEF\uff09\u4f5c\u4e3a\u9972\u517b\u5c42\uff0c\u5e76\u4f7f\u7528\u6dfb\u52a0\u80ce\u725b\u8840\u6e05\uff08FBS\uff09\u7684\u57f9\u517b\u57fa\u8fdb\u884c\u57f9\u517b\u3002\u8fd9\u9879\u5f00\u521b\u6027\u5de5\u4f5c\u6210\u4e3a\u4e86\u5f00\u53d1\u5404\u79cd PSC \u57f9\u517b\u7cfb\u7edf\u7684\u8d77\u70b9\u3002\u6b64\u540e\u7814\u7a76\u4eba\u5458\u5728\u7b80\u5316\u57f9\u517b\u7cfb\u7edf\u65b9\u9762\u4ed8\u51fa\u4e86\u5de8\u5927\u7684\u52aa\u529b\uff0c\u6700\u7ec8\u5f00\u53d1\u51fa\u6765\u4e86\u4e0d\u4f9d\u8d56\u4e8e\u9972\u517b\u5c42\u7684\u57f9\u517b\u57fa\u548c\u57fa\u8d28\u7528\u4e8e\u57f9\u517b PSCs\u3002<\/p>\n\n\n\n

\u5728\u8fdb\u884c\u4eba\u591a\u80fd\u5e72\u7ec6\u80de\uff08hPSC\uff09\u57f9\u517b\u65f6\uff0c\u60a8\u662f\u5426\u8fd8\u5728\u4e3a\u9009\u62e9\u54ea\u79cd\u7ec6\u80de\u5916\u57fa\u8d28\u800c\u7ea0\u7ed3\uff1f\u672c\u6587\u5c06\u5e26\u4f60\u6df1\u5165\u4e86\u89e3\u76ee\u524d\u4e3b\u6d41\u7684\u4e09\u79cdhPSC\u65e0\u9972\u517b\u5c42\u57fa\u8d28\uff1a<\/p>\n\n\n\n

Geltrex Flex \u57fa\u8d28<\/strong><\/h2>\n\n\n\n

\u5728hPSC\u57fa\u7840\u7814\u7a76\u9886\u57df\uff0c\u6765\u6e90\u4e8e\u5c0f\u9f20EHS\u80bf\u7624\u7684\u57fa\u5e95\u819c\u63d0\u53d6\u7269\uff08BME\uff09\u662f\u7ecf\u5178\u7684\u7ec6\u80de\u5916\u57fa\u8d28\u3002Gibco Geltrex Flex \u57fa\u8d28\u5373\u5c5e\u4e8e\u6b64\u7c7b\uff0c\u5176\u4e3b\u8981\u6210\u5206\u5305\u62ec\u5c42\u7c98\u8fde\u86cb\u767d\u3001IV\u578b\u80f6\u539f\u3001\u5de2\u86cb\u767d\u548c\u786b\u9178\u809d\u7d20\u86cb\u767d\u805a\u7cd6\u3002<\/p>\n\n\n\n

\u7ecfhESC\u8ba4\u8bc1\u7684Geltrex Flex\u57fa\u8d28\u53ef\u7528\u4e8e\u4eba\u80da\u80ce\u5e72\u7ec6\u80de\uff08hESCs\uff09\u548c\u8bf1\u5bfc\u591a\u80fd\u5e72\u7ec6\u80de\uff08iPSCs\uff09\u7684\u8d34\u58c1\u4e0e\u7ef4\u6301\u57f9\u517b\u3002\u6bcf\u6279\u5747\u4f7f\u7528iPSC\u548cH9 hESC\u8fdb\u884c\u4e86\u81f3\u5c11\u4e94\u4ee3\u57f9\u517b\u6d4b\u8bd5\uff0c\u5e76\u51cf\u4f4e\u751f\u957f\u56e0\u5b50\u542b\u91cf\uff0c\u4ee5\u786e\u4fddhESC\/iPSC\u7814\u7a76\u7684\u53ef\u9760\u6027\u4e0e\u4e00\u81f4\u6027\u3002<\/strong><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe1. Geltrex Flex \u652f\u6301 hESC \u4eba\u80da\u80ce\u5e72\u7ec6\u80de\u7684\u7684\u9ad8\u6548\u751f\u957f\u3001\u591a\u80fd\u6027\u7ef4\u6301\uff1a\u4f7f\u7528 StemFlex \u57f9\u517b\u57fa\u3001\u5206\u522b\u5305\u88ab Geltrex Flex\u3001Geltrex \u548c C\u54c1\u724c\u7684\u57f9\u517b\u4f53\u7cfb\u4e2d\uff0c BS3C PSC \u7ef4\u6301\u81ea\u6211\u66f4\u65b0\u56e0\u5b50\u7684\u8868\u8fbe\u3002<\/p>\n\n\n\n

\u73bb\u8fde\u86cb\u767d\uff08Vitronectin, VTN-N\uff09<\/strong><\/h2>\n\n\n\n

\u57fa\u8d28\u80f6\u53ef\u6709\u6548\u652f\u6301\u4eba\u80da\u80ce\u5e72\u7ec6\u80de\u7684\u957f\u671f\u589e\u6b96\uff0c\u4f46\u5176\u6210\u5206\u662f\u590d\u6742\u7684\u5c0f\u9f20\u6e90\u86cb\u767d\u6df7\u5408\u7269\uff0c\u662f\u57f9\u517b\u4f53\u7cfb\u53d8\u5f02\u6027\u7684\u6765\u6e90\u4e4b\u4e00\u3002<\/p>\n\n\n\n

\u4e0e\u6807\u51c6\u7684\u57fa\u5e95\u819c\u63d0\u53d6\u7269 \uff08BMEs\uff09 \u4e0d\u540c\uff0c\u73bb\u8fde\u86cb\u767d \uff08VTN-N\uff09 \u662f\u4e00\u79cd\u91cd\u7ec4\u4eba\u86cb\u767d\uff0c\u53ef\u4e3a\u4eba\u591a\u80fd\u6027\u5e72\u7ec6\u80de \uff08PSCs\uff09 \u7684\u65e0\u6ecb\u517b\u5c42\u57f9\u517b\u63d0\u4f9b\u6210\u5206\u9650\u5b9a\u7684\u8868\u9762\u3002Gibco VTN-N\u53d8\u4f53\u57fa\u4e8e James Thomson \u5b9e\u9a8c\u5ba4\u8bbe\u8ba1\uff0c\u5df2\u88ab\u8bc1\u660e\u5728\u652f\u6301\u4eba\u591a\u80fd\u5e72\u7ec6\u80de\u8d34\u58c1\u548c\u5b58\u6d3b\u65b9\u9762\u4f18\u4e8e\u91ce\u751f\u578b\u73bb\u8fde\u86cb\u767d[2]<\/sup>\u3002\u4e0eEssential 8\u548cEssential 8 Flex \u57f9\u517b\u57fa\u7ed3\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5efa\u7acb\u6210\u5206\u786e\u5b9a\u4e14\u4e0d\u542b\u5916\u6e90\u6027\u6210\u5206\u7684\u57f9\u517b\u7cfb\u7edf\u3002<\/strong><\/p>\n\n\n\n

\u73bb\u8fde\u86cb\u767d \uff08VTN-N\uff09 \u5df2\u88ab\u8bc1\u660e\u53ef\u652f\u6301\u591a\u80fd\u5e72\u7ec6\u80de\u751f\u957f\u8d85\u8fc7 50 \u4ee3\u4e14\u65e0\u4efb\u4f55\u6838\u578b\u5f02\u5e38\u8ff9\u8c61\uff0c\u5e76\u80fd\u7ef4\u6301\u591a\u80fd\u5e72\u7ec6\u80de\u5206\u5316\u4e3a\u6240\u6709\u4e09\u4e2a\u80da\u5c42\u8c31\u7cfb\u7684\u80fd\u529b\u3002<\/p>\n\n\n\n

\u4e3a\u4e86\u786e\u4fdd\u4f9b\u5e94\u7684\u7a33\u5b9a\u6027\u548c\u53ef\u6301\u7eed\u6027\uff0c\u8d5b\u9ed8\u98de\u73b0\u5df2\u5c06RUO Vitronectin \u7684\u751f\u4ea7\u7ebf\u6210\u529f\u8f6c\u79fb\u81f3\u4f4d\u4e8e\u7acb\u9676\u5b9b\u7ef4\u5c14\u7ebd\u65af\u7684\u751f\u4ea7\u57fa\u5730\uff0c\u5e76\u540c\u6b65\u542f\u7528\u65b0\u8d27\u53f7 A400457 \u548c A400458\uff0c<\/strong>\u5176\u4ea7\u80fd\u5c06\u8fdb\u4e00\u6b65\u5f97\u5230\u63d0\u5347\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe2. \u4f7f\u7528VTN-N\u57f9\u517b\u7684iPSCs\u9ad8\u8868\u8fbe\u591a\u80fd\u6027\u6807\u5fd7\u7269\uff08\u5982OCT4\u3001SOX2\u3001TRA-1-60\u7b49\uff09: \u5229\u7528CytoTune-iPS\u4ed9\u53f0\u75c5\u6bd2\u91cd\u7f16\u7a0b\u8bd5\u5242\u76d2\u5c06BJ\u6210\u7ea4\u7ef4\u7ec6\u80de\u8bf1\u5bfc\u4e3a\u8bf1\u5bfc\u591a\u80fd\u6027\u5e72\u7ec6\u80de \uff08iPSCs\uff09\uff0c\u4f7f\u7528 Essential 8\u57f9\u517b\u57fa \uff08A1517001\uff09 \u548c 0.5 \u03bcg\/cm2 VTN-N\u57f9\u517b\u516b\u4ee3\u4ee5\u4e0a\u3002\u8fdb\u884c\u7ec6\u80de\u67d3\u8272\uff0c\u68c0\u6d4b\u5176\u591a\u80fd\u6027\u7684 \uff08A\uff09 \u7ec6\u80de\u5185\u6807\u8bb0\u7269\u548c \uff08B\uff09 \u8868\u9762\u6807\u8bb0\u7269\u3002<\/p>\n\n\n\n

\u4eba\u91cd\u7ec4\u5c42\u7c98\u8fde\u86cb\u767d\uff08rhLaminin-521\uff09<\/strong><\/h2>\n\n\n\n

\u65e0\u8bba\u662f\u6210\u5206\u590d\u6742\u4f46\u7ecf\u5178\u7684\u57fa\u8d28\u80f6\uff0c\u8fd8\u662f\u6210\u5206\u660e\u786e\u4e14\u9ad8\u6548\u7684Vitronectin\uff0c\u90fd\u4e3ahPSC\u57f9\u517b\u63d0\u4f9b\u4e86\u4f18\u79c0\u7684\u89e3\u51b3\u65b9\u6848\u3002\u7136\u800c\uff0c\u5728\u9762\u5bf9\u4e00\u4e9b\u6781\u5177\u6311\u6218\u6027\u7684\u5b9e\u9a8c\u573a\u666f\u65f6\uff0c\u7814\u7a76\u4eba\u5458\u9700\u8981\u66f4\u5f3a\u5927\u7684\u201c\u5229\u5668\u201d\uff0c\u90a3\u5c31\u662f\u63a5\u4e0b\u6765\u8981\u4ecb\u7ecd\u7684\u4eba\u91cd\u7ec4\u5c42\u7c98\u8fde\u86cb\u767d\uff08rhLaminin-521\uff09\u3002<\/p>\n\n\n\n

Laminin-521\u662f\u5929\u7136\u5e72\u7ec6\u80de\u5fae\u73af\u5883\u7684\u5173\u952e\u57fa\u5e95\u819c\u86cb\u767d\uff0c\u7531\u80da\u80ce\u7684\u5185\u90e8\u7ec6\u80de\u56e2\u4e2d\u7684hPSC\u8868\u8fbe\u548c\u5206\u6ccc\uff0c\u53ef\u4ee5\u652f\u6301hPSC\u7684\u7a33\u5b9a\u6269\u589e\u4ee5\u53ca\u968f\u540e\u7684\u7ec6\u80de\u8c31\u7cfb\u7279\u5316\u3002<\/p>\n\n\n\n

Gibco rhLaminin-521\u7ecf\u8bc1\u5b9e\u53ef\u5728\u538b\u529b\u6761\u4ef6\u4e0b\u63d0\u5347 PSC \u7684\u5b58\u6d3b\uff0c\u5373\u4f7f\u6ca1\u6709\u5c0f\u5206\u5b50 Rho \u76f8\u5173\u86cb\u767d\u6fc0\u9176 \uff08ROCK\uff09 \u6291\u5236\u5242\u5b58\u5728[3]\uff0c\u5982\u7528\u4e8e\u91cd\u7f16\u7a0b\u3001PSC \u4ece\u8f83\u4e30\u5bcc\u7684\u57f9\u517b\u57fa\u5230\u7cbe\u7b80\u578b\u57f9\u517b\u57fa\u7684\u5207\u6362\uff0c\u4ee5\u53ca\u8367\u5149\u6fc0\u6d3b\u7ec6\u80de\u5206\u9009 \uff08FACS\uff09 \u540e\u7684\u5355\u7ec6\u80de\u514b\u9686\u751f\u957f\u7b49\uff0c<\/strong>\u4e3a\u60a8\u7684hPSC\u57f9\u517b\u63d0\u4f9b\u5353\u8d8a\u7684\u6027\u80fd\u3002<\/p>\n\n\n\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u6211\u4eec\u4e3a\u5927\u5bb6\u6574\u7406\u4e86\u5982\u4e0b\u57fa\u8d28\u9009\u62e9\u573a\u666f\uff1a<\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

\u603b\u800c\u8a00\u4e4b\uff0c\u6ca1\u6709\u201c\u6700\u597d\u201d\u7684\u57fa\u8d28\uff0c\u53ea\u6709\u201c\u6700\u5408\u9002\u201d\u7684\u57fa\u8d28\u3002Geltrex Flex\u662f\u7ecf\u6d4e\u53ef\u9760\u7684\u7ecf\u5178\u4e4b\u9009\uff0cVitronectin\u662f\u6210\u5206\u660e\u786e\u4f53\u7cfb\u7684\u4e0d\u4e8c\u6cd5\u95e8\uff0c\u800cLaminin-521\u5219\u80fd\u52a9\u60a8\u653b\u514b\u6700\u96be\u7684\u5355\u7ec6\u80de\u514b\u9686\u4e0e\u57fa\u56e0\u7f16\u8f91\u6311\u6218\u3002<\/strong><\/p>\n\n\n\n

\u76f8\u5173\u4ea7\u54c1<\/strong><\/h2>\n\n\n\n
\"\"<\/a><\/figure>\n\n\n\n

\u8868\u6ce8\uff1aRUO (Research Use Only); CTS (Cell Therapy Systems)<\/p>\n\n\n\n

\u53c2\u8003\u6587\u732e\uff1a<\/h2>\n\n\n\n

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. \uff081998\uff09 Embryonic stem cell lines derived from human blastocysts. Science 282\uff085391\uff09:1145-1147.<\/p>\n\n\n\n

2. Chen G, Gulbranson DR, Hou Z et al. \uff082011\uff09 Chemically defined conditions for human iPSC derivation and culture. Nat Methods 8:424-429.<\/p>\n\n\n\n

3. Rodin S, Antonsson L, Hovatta O et al. \uff082014\uff09 Monolayer culturing and cloning of human pluripotent stem cells on Laminin-521-based matrices under xeno-free and chemically dened conditions. Nat Protoc 9\uff0810\uff09:2354-2368.<\/p>\n\n\n\n

\"contact<\/a><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

PSC \u57f9\u517b\u9700\u8981\u4e00\u6574\u5957\u517c\u5bb9\u7684\u57f9\u517b\u57fa\u3001\u57fa\u8d28\u548c\u4f20\u4ee3\u65b9\u6cd5\uff0c\u4ee5\u652f\u6301\u7ec6\u80de\u7684\u5065\u5eb7\u751f\u957f\u548c\u4fdd\u6301\u591a\u80fd\u6027\u3002Thomson \u7b49\u4eba\u5728 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