{"id":2068,"date":"2026-06-01T02:58:14","date_gmt":"2026-06-01T02:58:14","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=2068"},"modified":"2026-06-01T02:58:15","modified_gmt":"2026-06-01T02:58:15","slug":"whats-possible-cryo-electron-tomography","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/whats-possible-cryo-electron-tomography\/","title":{"rendered":"\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf &#8211; \u7ec6\u80de\u751f\u7269\u5b66 &#8211; \u539f\u5b50\u5206\u8fa8\u7387\u4e0b\u7684\u751f\u547d"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/communities-social\/socialhub\/blog-subscribe.html?icid=cn-blog-subscribe\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg\" alt=\"\" class=\"wp-image-2271\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p>\u4e00\u4e2a\u591a\u4e16\u7eaa\u524d\uff0c\u8457\u540d\u6570\u5b66\u5bb6\u7ea6\u7ff0\u00b7\u62c9\u4e1c&nbsp;Johan Radon\u4e3a\u65ad\u5c42\u91cd\u5efa\u63d0\u4f9b\u4e86\u6570\u5b66\u6846\u67b6\u3002\u591a\u5e74\u4ee5\u540e\uff0c\u57281968\u5e74\uff0c\u5927\u536b\u00b7\u5fb7\u7f57\u897f\u5c14David DeRosier\u548c\u963f\u4f26\u00b7\u514b\u5362\u683cAaron Klug<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23610788\/\"><u>\u53d1\u8868\u4e86\u4e00\u7bc7\u5177\u6709\u91cc\u7a0b\u7891\u610f\u4e49\u7684\u300a\u81ea\u7136\u300b\u8bba\u6587<\/u><\/a>\u9996\u6b21\u901a\u8fc7\u4e8c\u7ef4\u6295\u5f71\u91cd\u5efa\u51fa\u4e09\u7ef4\u7684\u566c\u83cc\u4f53\u5c3e\u90e8\u7ed3\u6784\u3002<\/p>\n\n\n\n<p>\u8fd9\u6807\u5fd7\u7740\u73b0\u4ee3\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u6280\u672f\u7684\u8bde\u751f\u2014\u2014\u8be5\u6280\u672f\u901a\u8fc7\u4f7f\u7528\u7535\u5b50\u675f\u7167\u5c04\u6837\u54c1\u5e76\u8bb0\u5f55\u4e0d\u540c\u89d2\u5ea6\u7684\u6295\u5f71\uff0c\u63ed\u793a\u7269\u4f53\u5185\u90e8\u7ed3\u6784\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u6280\u672f\"><strong><strong>\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u6280\u672f<\/strong><\/strong><\/h2>\n\n\n\n<p>\u5982\u4eca\uff0c\u7ecf\u8fc7\u51e0\u5341\u5e74\u7684\u65b9\u6cd5\u53d1\u5c55\uff0c\u5305\u62ec\u51b7\u51bb\u6280\u672f\u7684\u8fdb\u6b65\u4ee5\u83b7\u5f97\u6c34\u5408\u72b6\u6001\u4e0b\u5b8c\u6574\u7684\u751f\u7269\u5206\u5b50\uff0c\u5e76\u5728\u7535\u5b50\u663e\u5fae\u955c\u4e2d\u5b9e\u73b0\u975e\u7834\u574f\u6027\u7167\u5c04\u6761\u4ef6\uff0c\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u6280\u672f\u8fbe\u5230\u4e86\u65e0\u4e0e\u4f26\u6bd4\u7684\u5206\u8fa8\u7387\u6c34\u5e73\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.nature.com\/articles\/d41586-021-02904-w\"><u>\u5728\u300a\u81ea\u7136\u300b\u6742\u5fd7\u4e2d\u9605\u8bfb\u66f4\u591a\u5185\u5bb9\uff1a\u524d\u6240\u672a\u89c1\u7684\u7ec6\u80de\u79d8\u5bc6\u751f\u6d3b\u3002\u5c16\u7aef\u663e\u5fae\u6280\u672f\u8ba9\u7814\u7a76\u4eba\u5458\u80fd\u591f\u7aa5\u63a2\u7ec6\u80de\u5185\u90e8\uff0c\u5c3d\u89c8\u5176\u62e5\u6324\u800c\u7cbe\u5f69\u7684\u666f\u8c61\u3002<\/u><u>&gt;&gt;<\/u><\/a><\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2022\/04\/cryo-et-blog-june.jpg\" alt=\"Cryo-electron tomography reveals phase-separated protein degradation microcompartment\" class=\"wp-image-1820\" \/><\/figure>\n<\/div>\n\n\n<p><em>\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u63ed\u793a\u4e86\u4e00\u4e2a\u76f8\u5206\u79bb\u7684\u86cb\u767d\u8d28\u964d\u89e3\u5fae\u9694\u5ba4\uff0c\u5176\u4e2d\u86cb\u767d\u9176\u4f53\uff08\u7ea2\u8272\uff09\u805a\u96c6\u5728\u5185\u8d28\u7f51\u819c\u4e0a\uff08\u7ec6\u80de\u8d28\u6838\u7cd6\u4f53\uff1a\u6d45\u84dd\u8272\uff1b\u819c\u7ed3\u5408\u6838\u7cd6\u4f53\uff1a\u6df1\u84dd\u8272\uff1bCdc48\uff1a\u9ec4\u8272\uff1b\u819c\uff1a\u767d\u8272\uff1b\u86cb\u767d\u7ed3\u6784\u901a\u8fc7\u4e9a\u65ad\u5c42\u5e73\u5747\u83b7\u5f97\uff0c\u5e76\u968f\u540e\u6620\u5c04\u56de\u7ec6\u80de\u65ad\u5c42\u56fe\u50cf\u4e2d\uff09\u3002\u6570\u636e\u7531Helmholtz Zentrum M\u00fcnchen\u7684Benjamin Engel\u535a\u58eb\u63d0\u4f9b.<\/em><\/p>\n\n\n\n<p>\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u5f25\u5408\u4e86\u7ed3\u6784\u751f\u7269\u5b66\u65b9\u6cd5\u4e0e\u73b0\u6709\u5149\u5b66\u663e\u5fae\u6280\u672f\u4e4b\u95f4\u7684\u5dee\u8ddd\uff0c\u63d0\u4f9b\u4e86\u539f\u751f\u7ec6\u80de\u5185\u90e8\u7684\u4e09\u7ef4\u56fe\u50cf\u548c\u5feb\u7167\u3002<\/p>\n\n\n\n<p>\u5b83\u4f7f\u6211\u4eec\u80fd\u591f\u7814\u7a76\u86cb\u767d\u8d28\u548c\u5176\u4ed6\u5206\u5b50\u5982\u4f55\u534f\u540c\u5de5\u4f5c\u4ee5\u5b8c\u6210\u7ec6\u80de\u4e2d\u7684\u4e3b\u8981\u8fc7\u7a0b\uff0c\u6539\u53d8\u4e86\u6211\u4eec\u5bf9\u7ec6\u80de\u529f\u80fd\u7684\u8ba4\u77e5\uff0c\u5e76\u5e26\u6765\u4e86\u5176\u4ed6\u65b9\u6cd5\u65e0\u6cd5\u63d0\u4f9b\u7684\u65b0\u751f\u7269\u5b66\u6d1e\u89c1\u3002<\/p>\n\n\n\n<p>\u5f97\u76ca\u4e8e\u6280\u672f\u8fdb\u6b65\u3001\u6570\u5b57\u5316\u7a0b\u5ea6\u63d0\u5347\u4ee5\u53ca\u68c0\u6d4b\u9886\u57df\u7684\u53d8\u9769\u6027\u7a81\u7834\uff0c<a href=\"https:\/\/ter.li\/4ntjx3\"><u>\u6837\u54c1\u5236\u5907<\/u><\/a>\u548c\u81ea\u52a8\u5316\uff0c\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u53d1\u5c55\u5230\u4eca\u5929\uff1a\u5373\u5c06\u6210\u4e3a\u6211\u4eec\u6210\u50cf\u5de5\u5177\u7bb1\u4e2d\u7684\u57fa\u7840\u7ec4\u4ef6\uff0c\u5728\u7ec6\u80de\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u5177\u6709\u5de8\u5927\u6f5c\u529b\u3002<\/p>\n\n\n\n<p><em>\u63a2\u7d22\u6211\u4eec\u5168\u65b0\u7cbe\u9009\u79d1\u5b66\u51fa\u7248\u7269\u5408\u96c6\uff0c\u91cd\u70b9\u4ecb\u7ecd\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u7684\u5e94\u7528\u3002<\/em><a href=\"https:\/\/ter.li\/w6xnnf\"><u>\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u7535\u5b50\u4e66<\/u><\/a>\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662f\u53ef\u80fd\u7684-\u7ec6\u80de\u751f\u7269\u5b66\u7684\u65b0\u7eaa\u5143\"><strong><strong>\u4ec0\u4e48\u662f\u53ef\u80fd\u7684\uff1a\u7ec6\u80de\u751f\u7269\u5b66\u7684\u65b0\u7eaa\u5143<\/strong><\/strong><\/h2>\n\n\n\n<p>\u6211\u4e00\u76f4\u5bf9\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\u6280\u672f\u5728\u4e0d\u540c\u5e94\u7528\u9886\u57df\u5e26\u6765\u7684\u7814\u7a76\u6210\u679c\u611f\u5230\u7740\u8ff7\u3002<\/p>\n\n\n\n<p>\u6211\u4eec\u5df2\u7ecf\u4e86\u89e3\u4e86\u4ee5\u524d\u96be\u4ee5\u53ef\u89c6\u5316\u7684\u7ec6\u80de\u7ed3\u6784\uff0c\u4f8b\u5982<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31882451\/\"><u>\u4f4d\u4e8e\u5185\u8d28\u7f51\u819c\u4e0a\u7684\u964d\u89e3\u5fae\u533a\u5ba4<\/u><\/a>(2020)\uff1b<a href=\"https:\/\/www.nature.com\/articles\/s41586-020-2670-5\"><u>\u5177\u6709\u590d\u6742\u7ed3\u6784\u7684\u5927\u578b\u5927\u5206\u5b50\u590d\u5408\u4f53<\/u><\/a>\uff0c\u5982\u6838\u5b54\u590d\u5408\u4f53\uff0c\u73b0\u5728\u53ef\u4ee5\u5728\u5176\u7ec6\u80de\u73af\u5883\u4e2d\u8fdb\u884c\u9610\u91ca\uff082020\uff09\uff1b\u4ee5\u53ca\u63ed\u793a\u51fa\u7684\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u8d85\u5fae\u7ed3\u6784\u53d8\u5316\u2014\u2014\u6700\u8fd1\u7684\u4f8b\u5b50\u5305\u62ec\uff0c<a href=\"https:\/\/www.cell.com\/cell\/fulltext\/S0092-8674(20)30995-8\"><u>\u901a\u8fc7\u539f\u4f4d\u65ad\u5c42\u626b\u63cf\u89e3\u6790\u7684\u4e0e\u5e15\u91d1\u68ee\u75c5\u76f8\u5173\u7684\u6fc0\u9176\u7ed3\u6784<\/u><\/a>(2020)\uff0c\u6216\u8005<a href=\"https:\/\/www.cell.com\/cell\/fulltext\/S0092-8674(17)31510-6\"><u>\u795e\u7ecf\u6bd2\u6027\u86cb\u767d\u805a\u96c6\u4f53\u963b\u6ede\u4e86\u7ec6\u80de\u86cb\u767d\u964d\u89e3\u673a\u5236<\/u><\/a>\uff082018\u5e74\uff09\u3002<\/p>\n\n\n\n<p>\u6211\u4eec\u5728\u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280\u7684\u89d2\u8272\u662f\u901a\u8fc7\u6301\u7eed\u7684\u6280\u672f\u521b\u65b0\u5e2e\u52a9\u751f\u7269\u5b66\u5bb6\u4fdd\u6301\u8fd9\u4e00\u52bf\u5934\uff0c\u5e76\u786e\u4fdd\u4ed6\u4eec\u80fd\u591f\u5728\u6574\u4e2a\u7814\u7a76\u8fc7\u7a0b\u4e2d\u5145\u5206\u91ca\u653e\u51b7\u51bb\u7535\u955c\u7684\u6df1\u8fdc\u4ef7\u503c\u3002.<\/p>\n\n\n\n<p>\u6211\u4eec\u7684\u900f\u5c04\u7535\u955c\uff08TEM\uff09\uff0c\u5305\u62ec<a href=\"https:\/\/ter.li\/blgtrp\"><u>Thermo Scientific Krios G4 \u51b7\u51bb\u900f\u5c04\u7535<\/u><u>\u955c\uff08Cryo-TEM\uff09<\/u><\/a>\u4ee5\u53ca<a href=\"https:\/\/ter.li\/i0zopc\"><u>Glacios \u51b7\u51bb\u900f\u5c04\u7535<\/u><u>\u955c\uff08Cryo-TEM\uff09<\/u><\/a>\uff0c\u5b9e\u73b0\u4e86\u6bd4\u4ee5\u5f80\u66f4\u9ad8\u7684\u901a\u91cf\u548c\u81ea\u52a8\u5316\u6c34\u5e73\u3002\u540c\u65f6\uff0c\u51b7\u51bb\u805a\u7126\u79bb\u5b50\u675f\uff08Cryo-FIB\uff09\u6280\u672f\uff0c\u5305\u62ec<a href=\"https:\/\/ter.li\/q81ci4\"><u>Thermo Scientific Aquilos 2 \u51b7\u51bb\u805a\u7126\u79bb\u5b50\u675f<\/u><u>\u7535\u955c<\/u><u>\uff08Cryo-FIB\uff09<\/u><\/a>\uff0c\u5df2\u7ecf\u53d1\u5c55\u6210\u4e3a\u7528\u4e8e\u65ad\u5c42\u6210\u50cf\u6837\u54c1\u5236\u5907\u7684\u98a0\u8986\u6027\u65b9\u6cd5\uff0c\u2014\u4f7f\u751f\u7269\u5b66\u5bb6\u80fd\u591f\u901a\u8fc7\u5236\u5907\u51b7\u51bb\u8584\u7247\uff0c\u771f\u6b63\u4e3a\u89c2\u5bdf\u5904\u4e8e\u529f\u80fd\u72b6\u6001\u7684\u7ec6\u80de\u73af\u5883\u6253\u5f00\u7a97\u53e3\u3002\u3002<\/p>\n\n\n\n<p>\u6211\u4eec\u4e5f\u5f88\u9ad8\u5174\u5ba3\u5e03\u6211\u4eec\u5728\u51b7\u51bb\u7535\u955c\u9886\u57df\u7684\u6700\u65b0\u521b\u65b0\u2014\u2014<a href=\"https:\/\/ter.li\/7ftfdn\"><u>Thermo Scientific iFLM \u76f8\u5173\u7cfb\u7edf<\/u><\/a>\uff0c\u8fd9\u662f Aquilos 2 \u51b7\u51bb\u805a\u7126\u79bb\u5b50\u675f\u7535\u955c\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u4e5f\u662f\u6211\u4eec\u9996\u6b3e\u96c6\u6210\u8367\u5149\u5149\u5b66\u663e\u5fae\u955c\u3002<\/p>\n\n\n\n<p><a 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