{"id":240,"date":"2026-02-05T06:33:00","date_gmt":"2026-02-05T06:33:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=240"},"modified":"2026-03-10T06:05:47","modified_gmt":"2026-03-10T06:05:47","slug":"the-essential-guide-to-glutamine-in-cell-culture","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/the-essential-guide-to-glutamine-in-cell-culture\/","title":{"rendered":"L-\u8c37\u6c28\u9170\u80fa\u5728\u7ec6\u80de\u57f9\u517b\u4e2d\u7684\u4f5c\u7528\u53ca\u57fa\u672c\u6307\u5357"},"content":{"rendered":"\n<p>\u5728\u7ec6\u80de\u751f\u7269\u5b66\u9886\u57df\uff0c\u8425\u517b\u73af\u5883\u5bf9\u4e8e\u7ef4\u6301\u5f3a\u5065\u7684\u7ec6\u80de\u57f9\u517b\u81f3\u5173\u91cd\u8981\u3002\u5728\u8fd9\u4e9b\u5fc5\u9700\u8425\u517b\u7d20\u4e2d\uff0c\u8c37\u6c28\u9170\u80fa\u662f\u4e00\u79cd\u5173\u952e\u7684\u6c28\u57fa\u9178\uff0c\u53c2\u4e0e\u591a\u79cd\u7ec6\u80de\u8fc7\u7a0b\u3002\u65e0\u8bba\u4f60\u662f\u7814\u7a76\u4eba\u5458\u8fd8\u662f\u79d1\u5b66\u7231\u597d\u8005\uff0c\u4e86\u89e3\u8c37\u6c28\u9170\u80fa\u7684\u4f5c\u7528\u90fd\u80fd\u4e3a\u7ec6\u80de\u4ee3\u8c22\u548c\u5b9e\u9a8c\u4f18\u5316\u63d0\u4f9b\u5b9d\u8d35\u89c1\u89e3\u3002\u5728\u672c\u6587\u4e2d\uff0c\u6211\u4eec\u5c06\u6df1\u5165\u63a2\u8ba8\u8c37\u6c28\u9170\u80fa\u80cc\u540e\u7684\u79d1\u5b66\u539f\u7406\u3001\u5b83\u5728<a 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rel=\"noopener\">L-\u8c37\u6c28\u9170\u80fa<\/a>\u662f\u8c37\u6c28\u9170\u80fa\u7684L-\u5f02\u6784\u4f53\uff0c\u5177\u6709\u751f\u7269\u6d3b\u6027\uff0c\u5e76\u5bf9\u591a\u9879\u7ec6\u80de\u529f\u80fd\u81f3\u5173\u91cd\u8981\u3002\u5b83\u662f\u86cb\u767d\u8d28\u548c\u6838\u82f7\u9178\u5408\u6210\u7684\u91cd\u8981\u5e95\u7269\uff0c\u652f\u6301\u7ec6\u80de\u751f\u957f\u4e0e\u5206\u88c2\u3002L-\u8c37\u6c28\u9170\u80fa\u8fd8\u901a\u8fc7\u4fc3\u8fdb\u4e3b\u8981\u6297\u6c27\u5316\u5242\u2014\u2014\u8c37\u80f1\u7518\u80bd\u7684\u5408\u6210\uff0c\u6709\u52a9\u4e8e\u7ef4\u6301\u7ec6\u80de\u5185\u6c27\u5316\u8fd8\u539f\u5e73\u8861\u3002\u5728\u5feb\u901f\u5206\u88c2\u7684\u7ec6\u80de\uff08\u5982\u514d\u75ab\u7cfb\u7edf\u548c\u80a0\u4e0a\u76ae\uff09\u4e2d\uff0cL-\u8c37\u6c28\u9170\u80fa\u4f5c\u4e3a\u4e3b\u8981\u80fd\u91cf\u6765\u6e90\u3002\u56e0\u6b64\uff0c\u5b83\u6709\u52a9\u4e8e\u7ef4\u6301\u80a0\u9ecf\u819c\u5b8c\u6574\u6027\u5e76\u589e\u5f3a\u514d\u75ab\u7ec6\u80de\u529f\u80fd\uff0c\u662f\u6574\u4f53\u7ec6\u80de\u5065\u5eb7\u4e0e\u7a33\u6001\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u56e0\u5b50\u3002\n<\/p>\n\n\n\n<h2 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class=\"wp-block-image\">\n<figure class=\"aligncenter is-resized\"><a href=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-1-COL027471-molecular-d3-v2-1-scaled.jpg\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-1-COL027471-molecular-d3-v2-1-300x181.jpg\" alt=\"\u5c55\u793a L - \u8c37\u6c28\u9170\u80fa\u5206\u89e3\u4e3a\u6c28\u548c\u5421\u54af\u70f7\u916e\u7fa7\u9178\u7684\u5316\u5b66\u7ed3\u6784\uff0c\u5176\u5206\u89e3\u901f\u7387\u53d6\u51b3\u4e8e pH \u503c\u548c\u6e29\u5ea6\u3002\n\" class=\"wp-image-3581\" style=\"width:300px;height:auto\" \/><\/a><figcaption class=\"wp-element-caption\">\u56fe1. L-\u8c37\u6c28\u9170\u80fa\u4f1a\u81ea\u53d1\u5206\u89e3\u4e3a\u6c28\u548c\u5421\u54af\u70f7\u7fa7\u9178\uff0c\u5176\u5206\u89e3\u901f\u7387\u53d6\u51b3\u4e8epH\u503c\u548c\u6e29\u5ea6\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5982\u4f55\u4e3a\u6211\u7684\u7ec6\u80de\u83b7\u5f97\u7a33\u5b9a\u7684l-\u8c37\u6c28\u9170\u80fa\">\u5982\u4f55\u4e3a\u6211\u7684\u7ec6\u80de\u83b7\u5f97\u7a33\u5b9a\u7684L-\u8c37\u6c28\u9170\u80fa\uff1f<\/h2>\n\n\n\n<p>\n\u4e3a\u4e86\u51cf\u5c11L-\u8c37\u6c28\u9170\u80fa\u5728\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u7684\u4e0d\u7a33\u5b9a\u6027\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u4f7f\u7528\u8be5\u6c28\u57fa\u9178\u7684\u7a33\u5b9a\u5f62\u5f0f\u3002\u4e00\u79cd\u6709\u6548\u7684\u65b9\u6cd5\u662f<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/media-supplements\/glutamax-media.html?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog3\" target=\"_blank\" rel=\"noopener\">Gibco\u2122 GlutaMAX\u2122 \u8865\u5145\u5242<\/a>\uff0c\u5b83\u542b\u6709\u4e8c\u80bd\u5f62\u5f0f\u7684L-alanyl-L-glutamine\u3002\u8fd9\u79cd\u7a33\u5b9a\u5f62\u5f0f\u80fd\u591f\u62b5\u6297\u964d\u89e3\uff0c\u5373\u4f7f\u572837\u00b0C\u4e0b\u957f\u671f\u5b75\u80b2\uff0c\u4e5f\u80fd\u663e\u8457\u51cf\u5c11\u6c28\u7684\u4ea7\u751f\uff08\u89c1\u56fe2\uff09\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><a href=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-2-A-B-2-1.png\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-2-A-B-2-1-1024x427.png\" alt=\"\u6298\u7ebf\u56fe 2A \u663e\u793a\uff0c\u5728 L - \u8c37\u6c28\u9170\u80fa\u6301\u7eed\u964d\u89e3\u7684\u60c5\u51b5\u4e0b\uff0cGlutaMAX \u8865\u5145\u5242\u5728 7 \u5929\u5185\u7684\u7a33\u5b9a\u6027\u3002\u6298\u7ebf\u56fe 2B \u663e\u793a\uff0c\u5728\u6dfb\u52a0\u4e86 L - \u8c37\u6c28\u9170\u80fa\u7684\u57f9\u517b\u57fa\u4e2d\uff0c\u6c28\u6d53\u5ea6\u5728 7 \u5929\u5185\u7a33\u6b65\u4e0a\u5347\u3002\n\" class=\"wp-image-3582\" \/><\/a><figcaption class=\"wp-element-caption\">\u56fe2\u3002A)\u572837\u00b0C\u6761\u4ef6\u4e0b\uff0cL-\u8c37\u6c28\u9170\u80fa\u5728\u57f9\u517b\u57fa\u4e2d\u7684\u964d\u89e3\u901f\u5ea6\u5feb\u4e8eGlutaMAX\u8865\u5145\u5242\u3002\u5c06DMEM\u5206\u522b\u6dfb\u52a0GlutaMAX\u8865\u5145\u5242\u6216L-\u8c37\u6c28\u9170\u80fa\uff0c\u5206\u88c5\u5230\u5c0f\u74f6\u4e2d\uff0c\u5e76\u50a8\u5b58\u572837\u00b0C\u3002\u6bcf\u5929\u53d6\u6837\u5e76\u51b7\u51bb\u4e8e\u201320\u00b0C\u3002\u901a\u8fc7\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6cd5\uff08HPLC\uff09\u6d4b\u5b9aGlutaMAX\u8865\u5145\u5242\u548cL-\u8c37\u6c28\u9170\u80fa\u7684\u542b\u91cf\u3002B) \u8865\u5145\u57f9\u517b\u57fa\u4e2d\u7684\u6c28\u6c34\u5e73\u3002\u5c06DMEM\u5206\u522b\u6dfb\u52a0GlutaMAX\u8865\u5145\u5242\u6216L-\u8c37\u6c28\u9170\u80fa\uff0c\u5206\u88c5\u5230\u5c0f\u74f6\u4e2d\uff0c\u5e76\u50a8\u5b58\u572837\u00b0C\u3002\u6bcf\u5929\u53d6\u6837\u5e76\u51b7\u51bb\u4e8e\u201320\u00b0C\u3002\u901a\u8fc7\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6cd5\uff08HPLC\uff09\u6d4b\u5b9a\u6c28\u7684\u542b\u91cf\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<p>\u5f53\u7ec6\u80de\u9700\u8981L-\u8c37\u6c28\u9170\u80fa\u65f6\uff0c\u5b83\u4eec\u80fd\u591f\u901a\u8fc7\u9176\u4fc3\u53cd\u5e94\u5c06\u7a33\u5b9a\u7684\u4e8c\u80bd\u5206\u89e3\u4e3a\u5355\u72ec\u7684L-\u8c37\u6c28\u9170\u80fa\u548cL-\u4e19\u6c28\u9178\uff0c\u7136\u540e\u4ee3\u8c22\u5229\u7528\u5b83\u4eec\u4ee5\u652f\u6301\u591a\u79cd\u9014\u5f84\u548c\u529f\u80fd\uff08\u89c1\u56fe3\uff09\u3002\u4f7f\u7528GlutaMAX\u8865\u5145\u5242\u53ef\u4ee5\u63d0\u9ad8\u7ec6\u80de\u57f9\u517b\u57fa\u7684\u7a33\u5b9a\u6027\uff0c\u786e\u4fdd\u7ec6\u80de\u8868\u73b0\u7684\u4e00\u81f4\u6027\uff0c\u5e76\u5ef6\u957f\u57f9\u517b\u7269\u7684\u5b58\u6d3b\u671f\uff0c\u4ece\u800c\u4f18\u5316\u5b9e\u9a8c\u7ed3\u679c\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><a href=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-3-Controlled-delivery-of-lglutamine.jpg\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/10\/Blog-Figure-3-Controlled-delivery-of-lglutamine-300x300.jpg\" alt=\"\u663e\u793a\u5728\u7ec6\u80de\u57f9\u517b\u4e2d L - \u8c37\u6c28\u9170\u80fa\u5206\u89e3\u4e3a PCA \u548c\u6c28\uff0c\u800c\u8c37\u6c28\u9170\u80fa\u8865\u5145\u5242\u53ef\u4f7f L - \u8c37\u6c28\u9170\u80fa\u66f4\u53ef\u63a7\u5730\u8f93\u9001\u5230\u7ec6\u80de\u4e2d\uff0c\u4e14\u4e0d\u4f1a\u4ea7\u751f\u8fd9\u4e9b\u6709\u6bd2\u526f\u4ea7\u7269\u7684\u56fe\u8868\n\" class=\"wp-image-3585\" \/><\/a><figcaption class=\"wp-element-caption\">\u56fe3. GlutaMAX\u8865\u5145\u5242\u53ef\u5b9e\u73b0\u4ece\u57f9\u517b\u57fa\u5411\u57f9\u517b\u7ec6\u80de\u4e2d\u53d7\u63a7\u91ca\u653eL-\u8c37\u6c28\u9170\u80fa\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<p>\u603b\u4e4b\uff0c\u4e86\u89e3L-\u8c37\u6c28\u9170\u80fa\u5728\u7ec6\u80de\u57f9\u517b\u4e2d\u7684\u4f5c\u7528\u53ca\u5176\u7a33\u5b9a\u6027\uff0c\u5bf9\u4e8e\u4f18\u5316\u7ec6\u80de\u751f\u957f\u548c\u5b9e\u9a8c\u7ed3\u679c\u81f3\u5173\u91cd\u8981\u3002\u9009\u62e9\u5982GlutaMAX\u8865\u5145\u5242\u7b49\u7a33\u5b9a\u66ff\u4ee3\u54c1\uff0c\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u83b7\u5f97\u66f4\u5065\u5eb7\u3001\u66f4\u9ad8\u4ea7\u7684\u7ec6\u80de\u57f9\u517b\u4f53\u7cfb\u3002\u5982\u9700\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u5982\u4f55\u5229\u7528\u542b\u6709GlutaMAX\u8865\u5145\u5242\u7684\u57f9\u517b\u57fa\u6700\u5927\u5316\u60a8\u7684\u7ec6\u80de\u57f9\u517b\u7684\u4fe1\u606f\uff0c\u8bf7\u8bbf\u95ee\uff1a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/media-supplements\/glutamax-media.html?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog4\" target=\"_blank\" rel=\"noopener\">thermofisher.com\/glutamax<\/a>\u3002<br><br>\n\n<strong>\u76f8\u5173\u5185\u5bb9\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u67e5\u627e\u542b\u6709 GlutaMAX \u7684\u57f9\u517b\u57fa<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media\/gibco-media-formulation-tool.html?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog6\" target=\"_blank\" rel=\"noopener\">Gibco \u57f9\u517b\u57fa\u9009\u62e9\u5de5\u5177<\/a><\/li>\n\n\n\n<li>\u641c\u7d22\u60a8\u611f\u5174\u8da3\u7684\u7ec6\u80de\u7cfb\uff0c\u4f7f\u7528<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-workflow-solutions\/cell-culture-select-tool.html?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog7\" target=\"_blank\" rel=\"noopener\">\uf0b7\u7ec6\u80de\u57f9\u517b\u9009\u62e9\u5de5\u5177<\/a><\/li>\n\n\n\n<li>\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u6211\u4eec\u7684\u57f9\u517b\u57fa\u3001\u80ce\u725b\u8840\u6e05\uff08FBS\uff09\u548c\u5851\u6599\u5236\u54c1\u5982\u4f55\u7ecf\u8fc7\u9a8c\u8bc1\uff0c\u4ee5\u786e\u4fdd\u5353\u8d8a\u7684\u4ea7\u54c1\u6027\u80fd\uff0c\u4ece\u800c\u8ba9\u60a8\u53ef\u4ee5<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-workflow-solutions.html?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog8\" target=\"_blank\" rel=\"noopener\">\u653e\u5fc3\u8fdb\u884c\u7ec6\u80de\u57f9\u517b<\/a>\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u6765\u6e90\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Yoo HC, Yu YC, Sung Y, Han JM. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32943735\/\" target=\"_blank\" rel=\"noopener\">\u7ec6\u80de\u4ee3\u8c22\u4e2d\u7684\u8c37\u6c28\u9170\u80fa\u4f9d\u8d56\u6027\u7814\u7a76<\/a>&#8220;. Exp Mol Med. 2020\u5e749\u6708;52(9):1496-1516. doi: 10.1038\/s12276-020-00504-8. 2020\u5e749\u670817\u65e5\u5728\u7ebf\u53d1\u8868\u3002PMID: 32943735; PMCID: PMC8080614.&#8221;<\/li>\n\n\n\n<li><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/brochures\/glutamax-product-brochure.pdf?cid=bid_clb_cce_r01_co_cp1610_pjt12011_bidglublg_0so_blg_il_awa_og_s00_glutamineblog5\" target=\"_blank\" rel=\"noopener\">Gibco GlutaMAX \u8865\u5145\u5242\u5ba3\u4f20\u518c<\/a><\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" 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