{"id":249,"date":"2026-02-10T05:45:17","date_gmt":"2026-02-10T05:45:17","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=249"},"modified":"2026-03-10T06:19:22","modified_gmt":"2026-03-10T06:19:22","slug":"western-blot-or-elisa","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/western-blot-or-elisa\/","title":{"rendered":"ELISA\u4e0eWestern Blot\u6bd4\u8f83\uff1a\u4f55\u65f6\u4f7f\u7528\u6bcf\u79cd\u514d\u75ab\u5206\u6790\u6280\u672f"},"content":{"rendered":"\n<h2 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\"><figure><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-2459 size-large\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/01\/elisawestern-1024x576.jpg\" alt=\"ELISA vs Western Blot\" width=\"760\" height=\"428\"><\/figure><\/h2>\n\n\n\n<h2 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u76ee\u5f55\"><strong>\u76ee\u5f55<\/strong><\/h2>\n\n\n\n<p><a href=\"#intro\">\u5f15\u8a00<\/a><br><a href=\"#whatelisa\">\u4ec0\u4e48\u662fELISA\uff1f<\/a><br><a href=\"#prosconselisa\">ELISA\u7684\u4f18\u70b9<\/a><br><a href=\"#prosconselisa\">ELISA\u7684\u7f3a\u70b9<\/a><br><a href=\"#whatwestern\">\u4ec0\u4e48\u662f\u86cb\u767d\u8d28\u5370\u8ff9\uff08western blot\uff09\uff1f<\/a><br><a href=\"#prosconswestern\">\u86cb\u767d\u8d28\u5370\u8ff9\u7684\u4f18\u70b9<\/a><br>\n<a href=\"#prosconswestern\">\u86cb\u767d\u8d28\u5370\u8ff9\u7684\u7f3a\u70b9<\/a><br><a href=\"#whenelisa\">\u4f55\u65f6\u4f7f\u7528ELISA\u8fdb\u884c\u86cb\u767d\u5206\u6790<\/a><br><a href=\"#whenwestern\">\u4f55\u65f6\u4f7f\u7528\u86cb\u767d\u8d28\u5206\u6790\u7684\u897f\u65b9\u5370\u8ff9\u6cd5\uff08Western Blot\uff09<\/a><br><a href=\"#more\">\u66f4\u591a\u5de5\u5177\u548c\u8d44\u6e90<\/a><br><a href=\"#more\">\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff08ELISA\uff09<\/a><br>\n<a href=\"#more\">\u897f\u65b9\u5370\u8ff9\u6cd5\uff08Western Blotting\uff09<\/a><\/p>\n\n\n\n<p><em>\u4f5c\u8005\uff1aFrances Gatta<\/em><\/p>\n\n\n\n<p id=\"intro\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogELISA\">\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff08ELISA\uff09<\/a>\u548c<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstrn\">\u897f\u65b9\u5370\u8ff9\u6cd5\uff08Western Blot\uff09<\/a>\u662f\u8bc6\u522b\u3001\u5b9a\u91cf\u548c\u5206\u6790\u86cb\u767d\u8d28\u7684\u5b9d\u8d35\u65b9\u6cd5\u3002<br><br>\nELISA\u662f\u4e00\u79cd\u9ad8\u5ea6\u7075\u654f\u3001\u7279\u5f02\u4e14\u591a\u529f\u80fd\u7684\u6280\u672f\uff0c\u80fd\u591f\u5728\u590d\u6742\u751f\u7269\u6837\u672c\u4e2d\u68c0\u6d4b\u5230\u7eb3\u6469\u5c14\u6d53\u5ea6\u7684\u86cb\u767d\u8d28\u3002\u8be5\u65b9\u6cd5\u64cd\u4f5c\u76f8\u5bf9\u7b80\u5355\uff0c\u5e76\u53ef\u540c\u65f6\u5206\u6790\u5927\u91cf\u6837\u672c\u3002\u7136\u800c\uff0c\u4e0e\u897f\u65b9\u5370\u8ff9\u6cd5\u76f8\u6bd4\uff0cELISA\u66f4\u5bb9\u6613\u4ea7\u751f\u5047\u9633\u6027\u6216\u5047\u9634\u6027\u7ed3\u679c\uff0c\u5e76\u4e14\u65e0\u6cd5\u63d0\u4f9b\u5173\u4e8e\u76ee\u6807\u86cb\u767d\u7684\u5168\u9762\u4fe1\u606f\u3002<br><br>\n\n\u53e6\u4e00\u65b9\u9762\uff0c\u867d\u7136\u86cb\u767d\u8d28\u5370\u8ff9\uff08western blotting\uff09\u6d41\u7a0b\u8f83\u4e3a\u590d\u6742\uff0c\u4f46\u901a\u5e38\u80fd\u591f\u63d0\u4f9b\u6e05\u6670\u4e14\u6613\u4e8e\u89e3\u91ca\u7684\u7ed3\u679c\u3002\u5b83\u5e38\u88ab\u7528\u4f5c\u5bf9ELISA\u53ca\u5176\u4ed6\u68c0\u6d4b\u65b9\u6cd5\u6240\u5f97\u7ed3\u679c\u7684\u786e\u8ba4\u5de5\u5177\u3002\n<\/p>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4ec0\u4e48\u662felisa-\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\"><strong>\u4ec0\u4e48\u662fELISA\uff08\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff09\uff1f<\/strong><\/h3>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogELISA\">\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a<\/a>\u6216\u79f0ELISA\uff0c\u662f\u4e00\u79cd\u9ad8\u5ea6\u7075\u654f\u548c\u7279\u5f02\u6027\u7684\u9176\u514d\u75ab\u5206\u6790\u6280\u672f\uff0c\u53ef\u7528\u4e8e\u5b9a\u91cf\u548c\u5b9a\u6027\u5206\u6790\u6297\u4f53\u6216\u6297\u539f\uff0c\u5305\u62ec\u86cb\u767d\u8d28\u3001\u6fc0\u7d20\u3001\u80bd\u3001\u6838\u9178\u7b49\u3002<br><br>\n\nELISA\u572896\u5b54\u805a\u82ef\u4e59\u70ef\u5438\u9644\u677f\u4e0a\u8fdb\u884c\u3002\u8fd9\u4e9b\u677f\u53ef\u4ee5\u4f7f\u6297\u4f53\u6216\u6297\u539f\u9ecf\u9644\uff0c\u5e76\u5141\u8bb8\u5728\u540c\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u7b5b\u67e5\u5927\u91cf\u6837\u672c\u3002<br><br>\n\nELISA\u53ef\u6309\u7167\u4ee5\u4e0b\u6b65\u9aa4\u8fdb\u884c\uff1a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogELISA\">\u56db\u4e2a\u57fa\u672c\u6b65\u9aa4<\/a>\uff1a\u5c06\u6297\u4f53\u6216\u6297\u539f\u5305\u88ab\u5230\u677f\u4e0a\uff0c\u7528\u725b\u8840\u6e05\u767d\u86cb\u767d\uff08BSA\uff09\u6216\u5176\u4ed6\u5206\u5b50\u5c01\u95ed\uff0c\u52a0\u5165\u80fd\u4ea7\u751f\u989c\u8272\u7684\u5e95\u7269\u8fdb\u884c\u68c0\u6d4b\uff0c\u5e76\u8bfb\u53d6\u7ed3\u679c\u3002<br><br>\n\nELISA\u6709<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogELISA\">\u56db\u79cd\u4e3b\u8981\u7c7b\u578b<\/a>, \u5305\u62ec\n<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>\u76f4\u63a5ELISA\uff1a<\/strong>\u5728\u8fd9\u91cc\uff0c\u4f7f\u7528\u5305\u88ab\u6297\u539f\u7684\u677f\u6765\u68c0\u6d4b\u6297\u4f53\u3002<\/li>\n\n\n\n<li><strong>\u95f4\u63a5ELISA\uff1a<\/strong>\u6b64\u7c7b\u578b\u4e2d\uff0c\u4f7f\u7528\u5305\u88ab\u6297\u539f\u7684\u677f\u6765\u7b5b\u67e5\u6297\u539f\u6216\u6297\u4f53\u3002<\/li>\n\n\n\n<li><strong>\u5939\u5fc3ELISA\uff1a<\/strong>3.\u5b83\u5229\u7528\u5305\u88ab\u6297\u4f53\u7684\u677f\u7b5b\u67e5\u6297\u539f\u3002\u6297\u539f\u5939\u5728\u6355\u83b7\u6297\u4f53\u548c\u68c0\u6d4b\u6297\u4f53\u4e4b\u95f4\uff0c\u56e0\u6b64\u5f97\u540d\u201c\u5939\u5fc3\u201d\u3002<\/li>\n\n\n\n<li><strong>\u7ade\u4e89\u6027ELISA\uff1a<\/strong>\u8be5\u68c0\u6d4b\u7528\u4e8e\u8bc6\u522b\u9488\u5bf9\u5f85\u6d4b\u8840\u6e05\u4e2d\u6297\u539f\u7279\u5f02\u6027\u7684\u6297\u4f53\u3002<\/li>\n<\/ol>\n\n\n<div class=\"wp-block-image size-full wp-image-2462\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/01\/elisa1.png\" alt=\"Diagram of common ELISA formats (direct vs. sandwich assays). In the assay, the antigen of interest is immobilized by direct adsorption to the assay plate or by first attaching a capture antibody to the plate surface. Detection of the antigen can then be performed using an enzyme-conjugated primary antibody (direct detection) or a matched set of unlabeled primary and conjugated secondary antibodies (indirect detection).\" class=\"wp-image-2462\" \/><figcaption class=\"wp-element-caption\">\u5e38\u89c1ELISA\u683c\u5f0f\uff08\u76f4\u63a5\u4e0e\u5939\u5fc3\u6cd5\uff09\u793a\u610f\u56fe\u3002\u5728\u5b9e\u9a8c\u4e2d\uff0c\u76ee\u6807\u6297\u539f\u53ef\u901a\u8fc7\u76f4\u63a5\u5438\u9644\u5230\u5b9e\u9a8c\u677f\u4e0a\u6216\u5148\u5c06\u6355\u83b7\u6297\u4f53\u56fa\u5b9a\u4e8e\u677f\u9762\u5b9e\u73b0\u56fa\u5b9a\u3002\u968f\u540e\u53ef\u901a\u8fc7\u9176\u6807\u8bb0\u7684\u4e00\u6297\uff08\u76f4\u63a5\u68c0\u6d4b\uff09\u6216\u4e00\u7ec4\u5339\u914d\u7684\u672a\u6807\u8bb0\u4e00\u6297\u548c\u9176\u6807\u4e8c\u6297\uff08\u95f4\u63a5\u68c0\u6d4b\uff09\u5bf9\u76ee\u6807\u6297\u539f\u8fdb\u884c\u68c0\u6d4b\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-elisa\u7684\u4f18\u7f3a\u70b9\"><strong>ELISA\u7684\u4f18\u7f3a\u70b9<\/strong><\/h3>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4f18\u70b9\"><strong>\u4f18\u70b9<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u8fd9\u662f\u4e00\u79cd<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5775980\/\">\u9ad8\u6548\u8282\u7701\u65f6\u95f4<\/a>\u7684\u65b9\u6cd5\uff0c\u7528\u4e8e\u5224\u65ad\u6837\u54c1\u4e2d\u662f\u5426\u5b58\u5728\u86cb\u767d\u8d28\u3002<\/li>\n\n\n\n<li>\u64cd\u4f5c\u6d41\u7a0b\u7b80\u5355\u5feb\u6377\uff0c\u51e0\u4e4e\u4e0d\u9700\u8981\u590d\u6742\u7684\u6837\u54c1\u5236\u5907\u3002<\/li>\n\n\n\n<li>\u4f5c\u4e3a\u4e00\u79cd\u66f4\u7075\u654f\u7684\u514d\u75ab\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u4ee5\u68c0\u6d4b\u5230\u5355\u4e00\u86cb\u767d\u6216\u6837\u54c1\u4e2d\u4f4e\u6d53\u5ea6\u86cb\u767d\u3002<\/li>\n\n\n\n<li>\u53ef\u4ee5\u540c\u65f6\u8fdb\u884c\u591a\u9879\u5206\u6790\u3002<\/li>\n\n\n\n<li>\u901a\u5e38\u4f7f\u7528\u7684\u662f<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/antibodies\/immunoassays\/elisa-kits.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogKits\">\u6210\u672c\u8f83\u4f4e\u7684\u8bd5\u5242<\/a>, \u4f7f\u5176\u5bf9\u9884\u7b97\u6709\u9650\u7684\u60c5\u51b5\u4e0b\u5177\u6709\u6210\u672c\u6548\u76ca\uff0c\u5c24\u5176\u9002\u5408\u6709\u7ecf\u9a8c\u7684\u7528\u6237\u9009\u62e9\u672a\u5305\u88ab\u7684ELISA\u8bd5\u5242\u76d2\u3002<\/li>\n\n\n\n<li>\u5b83\u662f\u5b9a\u91cf\u86cb\u767d\u6d53\u5ea6\u6216\u76d1\u6d4b\u86cb\u767d\u6c34\u5e73\u968f\u65f6\u95f4\u53d8\u5316\u7684\u6781\u4f73\u5de5\u5177\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-nbsp-\u7f3a\u70b9\"><strong>&nbsp;<\/strong><strong>\u7f3a\u70b9<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u7531\u4e8e\u6280\u672f\u9519\u8bef\u3001\u6837\u672c\u6c61\u67d3\u3001\u4f4e\u8d28\u91cf\u8bd5\u5242\u7b49\u539f\u56e0\uff0c\u5176\u7279\u5f02\u6027\u6709\u9650\uff0c\u66f4\u5bb9\u6613\u51fa\u73b0\u5047\u9633\u6027\u6216\u5047\u9634\u6027\u7ed3\u679c\u3002<\/li>\n\n\n\n<li>ELISA\u8bd5\u5242\u76d2\u4e2d\u7684\u6297\u4f53\u5fc5\u987b\u51b7\u85cf\u8fd0\u8f93\u548c\u50a8\u5b58\uff0c\u56e0\u4e3a\u5b83\u4eec\u5bb9\u6613\u4e0d\u7a33\u5b9a\u3002<\/li>\n\n\n\n<li>\u5b83\u4e0d\u80fd\u7528\u4e8e\u83b7\u5f97\u5173\u4e8e\u76ee\u6807\u86cb\u767d\u5176\u4ed6\u4fe1\u606f\uff0c\u5982\u5176\u5206\u5b50\u8d28\u91cf\u3002<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading glfh_headerContainer\" id=\"h-nbsp\"><strong>&nbsp;<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4ec0\u4e48\u662f\u86cb\u767d\u8d28\u5370\u8ff9-western-blot\"><strong>\u4ec0\u4e48\u662f\u86cb\u767d\u8d28\u5370\u8ff9\uff08western blot\uff09\uff1f<\/strong><\/h3>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstrn\">\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u6cd5\uff08Western Blot\uff09<\/a>\uff0c\u4e5f\u79f0\u4e3a\u514d\u75ab\u5370\u8ff9\uff0c\u662f\u4e00\u79cd\u5e38\u89c4\u6280\u672f\uff0c\u7528\u4e8e\u68c0\u6d4b\u548c\u5b9a\u91cf\u76ee\u6807\u86cb\u767d\uff0c\u5305\u62ec\u590d\u6742\u6df7\u5408\u7269\u4e2d\u4f4e\u4e30\u5ea6\u86cb\u767d\u7684\u68c0\u6d4b\u3002<span class=\"TextRun Highlight SCXW65506220 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW65506220 BCX0\">\u5b83\u662f\u4e00\u79cd\u5b9d\u8d35\u7684\u68c0\u6d4b\u65b9\u6cd5\uff0c\u7528\u4e8e\u8bc6\u522b\u548c\u7406\u89e3\u86cb\u767d\u8d28\u7279\u5f81\u5143\u7d20\u3001\u86cb\u767d\u8d28-\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u3001\u4fee\u9970\u7b49\u3002 &nbsp;<\/span><\/span><br><br><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstrn\">Western blot\uff08\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\uff09<\/a>\u9996\u5148\u901a\u8fc7\u51dd\u80f6\u7535\u6cf3\u6839\u636e\u5206\u5b50\u91cf\u5c06\u6df7\u5408\u7269\u4e2d\u7684\u86cb\u767d\u8d28\u5206\u79bb\u5f00\u6765\u3002\u5206\u79bb\u540e\u7684\u86cb\u767d\u8d28\u4ece\u51dd\u80f6\u8f6c\u79fb\u5e76\u7ed3\u5408\u5230\u86cb\u767d\u7ed3\u5408\u819c\u4e0a\u3002\u6700\u540e\uff0c<a href=\"https:\/\/www.genome.gov\/genetics-glossary\/Western-Blot#:~:text=The%20method%20involves%20using%20gel,a%20radioactive%20or%20chemical%20tag\"><\/a><a href=\"https:\/\/www.genome.gov\/genetics-glossary\/Western-Blot#:~:text=The%20method%20involves%20using%20gel,a%20radioactive%20or%20chemical%20tag\">\u8be5\u819c<\/a>\u7528\u9488\u5bf9\u76ee\u6807\u86cb\u767d\u7684\u6297\u4f53\u8fdb\u884c\u63a2\u9488\u68c0\u6d4b\uff0c\u4ece\u800c\u5728\u819c\u8868\u9762\u5b9e\u73b0\u5bf9\u5176\u7684\u68c0\u6d4b\u3002<\/p>\n\n\n<div class=\"wp-block-image wp-image-2090 size-full\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2023\/09\/Blot.jpg\" alt=\"\u501f\u52a9iBright\u7684\u8367\u5149\u591a\u91cd\u68c0\u6d4b\u6280\u672f\uff0c\u4ece\u6837\u672c\u4e2d\u83b7\u53d6\u66f4\u591a\u6570\u636e\u3002\u591a\u91cd\u68c0\u6d4b\u53ef\u5728\u5355\u5f20\u5370\u8ff9\u819c\u4e0a\u6355\u83b7\u591a\u8fbe\u56db\u79cd\u86cb\u767d\u8d28\uff0c\u52a9\u529b\u5f00\u5c55\u66f4\u5177\u610f\u4e49\u548c\u4ee3\u8868\u6027\u7684\u5b9e\u9a8c\uff0c\u8fd9\u662f\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\uff08Western blot\uff09\u53d1\u8868\u8bba\u6587\u7684\u5fc5\u5907\u6761\u4ef6\u3002\" class=\"wp-image-2090\" \/><figcaption class=\"wp-element-caption\">\u4f7f\u7528iBright\u6210\u50cf\u7cfb\u7edf\u6355\u83b7\u7684\u8367\u5149\u897f\u65b9\u5370\u8ff9\u56fe\u50cf\u793a\u4f8b\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u897f\u65b9\u5370\u8ff9\u6cd5\u7684\u4f18\u7f3a\u70b9\"><strong>\u897f\u65b9\u5370\u8ff9\u6cd5\u7684\u4f18\u7f3a\u70b9<\/strong><\/h3>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4f18\u70b9-0\"><strong>\u4f18\u70b9<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5f53\u64cd\u4f5c\u5f97\u5f53\u65f6\uff0c\u5b83\u53ef\u4ee5\u63d0\u4f9b\u7cbe\u786e\u4e14\u6613\u4e8e\u89e3\u91ca\u7684\u7ed3\u679c\u3002<\/li>\n\n\n\n<li>\uf0b7\u5b83\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\uff0c\u80fd\u591f\u4ece\u6210\u5343\u4e0a\u4e07\u79cd\u86cb\u767d\u8d28\u4e2d\u8bc6\u522b\u76ee\u6807\u86cb\u767d\u3002\u5728\u5982\u88c2\u89e3\u6db2\u7b49\u590d\u6742\u6837\u672c\u4e2d\u3002<span class=\"NormalTextRun SCXW225903799 BCX0\"><\/span><\/li>\n\n\n\n<li><span class=\"TextRun Highlight SCXW136778247 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW136778247 BCX0\">\u5b83\u53ef\u4ee5\u5728\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u63d0\u4f9b\u76ee\u6807\u86cb\u767d\u5206\u5b50\u91cf\u7684\u4fe1\u606f\uff0c<\/span><span class=\"NormalTextRun SCXW136778247 BCX0\">\u540c\u65f6\u8fd8\u80fd\u8bc6\u522b\u5fae\u5c0f\u4fee\u9970\uff0c\u5e76\u4e3a\u53ef\u80fd\u5177\u6709\u76f8\u4f3c\u6027\u8d28\u7684\u86cb\u767d\u8d28\u63d0\u4f9b\u53ef\u89c6\u5316\u8bc6\u522b\u3002<\/span><\/span><span class=\"TextRun SCXW136778247 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW136778247 BCX0\">&nbsp;<\/span><\/span><span class=\"EOP SCXW136778247 BCX0\" data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:276}\">&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u7f3a\u70b9\"><strong>\u7f3a\u70b9<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5b83\u901a\u5e38\u66f4\u9002\u5408\u4f5c\u4e3a\u786e\u8ba4\u5de5\u5177\uff0c\u800c\u975e\u5b8c\u5168\u5b9a\u91cf\u5de5\u5177\u3002<\/li>\n\n\n\n<li>\u8fd9\u53ef\u80fd\u662f\u4e00\u9879\u6210\u672c\u9ad8\u6602\u4e14\u8017\u65f6\u7684\u64cd\u4f5c\u3002<\/li>\n\n\n\n<li><span class=\"TextRun Highlight SCXW105115166 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\">\u68c0\u6d4b\u4fe1\u53f7\u53ef\u4ee5\u51cf\u5f31\u8fc5\u901f\u5730\uff0c\u9664\u975e\u4f7f\u7528\u957f\u6548\u5e95\u7269\uff088-24\u5c0f\u65f6\uff09\u6216\u8367\u5149\u67d3\u6599\u3002<\/span><span class=\"TextRun SCXW105115166 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW105115166 BCX0\">&nbsp;<\/span><\/span><span class=\"EOP SCXW105115166 BCX0\" data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:276}\">&nbsp;<\/span><\/li>\n\n\n\n<li><span class=\"TextRun Highlight SCXW197393032 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\">\u5b83\u662f\u5bf9\u6837\u54c1\u5236\u5907\u4e2d\u7684\u6742\u8d28\u66f4\u4e3a\u654f\u611f\uff0c\u8fd9\u4e9b\u6742\u8d28\u53ef\u80fd\u5bfc\u81f4<\/span><a class=\"Hyperlink SCXW197393032 BCX0\" href=\"https:\/\/www.future-science.com\/doi\/10.2144\/btn-2022-0003\" target=\"_blank\" rel=\"noreferrer noopener\">\u9ad8\u80cc\u666f\u566a\u97f3<\/a><span class=\"TextRun Highlight SCXW197393032 BCX0\" lang=\"EN\" xml:lang=\"EN\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW197393032 BCX0\"> or other <\/span><span class=\"NormalTextRun SCXW197393032 BCX0\">detection difficulties.<\/span><\/span><span class=\"EOP SCXW197393032 BCX0\" data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:276}\">&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-nbsp-0\"><strong>&nbsp;<\/strong><\/h3>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-elisa-\u4e0e\u86cb\u767d\u514d\u75ab\u5370\u8ff9-western-blot\"><strong>ELISA \u4e0e\u86cb\u767d\u514d\u75ab\u5370\u8ff9\uff08Western Blot\uff09<\/strong><\/h3>\n\n\n\n<p>\nELISA\u548c\u86cb\u767d\u8d28\u5370\u8ff9\uff08western blot\uff09\u662f\u4e24\u79cd\u5e38\u7528\u7684\u86cb\u767d\u8d28\u68c0\u6d4b\u4e0e\u5206\u6790\u65b9\u6cd5\u3002\u867d\u7136ELISA\u662f\u4e00\u79cd\u51fa\u8272\u7684\u86cb\u767d\u8d28\u68c0\u6d4b\u548c\u5b9a\u91cf\u5de5\u5177\uff0c\u4f46\u86cb\u767d\u8d28\u5370\u8ff9\u901a\u5e38\u7528\u4e8e\u786e\u8ba4ELISA\u6d4b\u8bd5\u7684\u7ed3\u679c\uff0c\u56e0\u4e3a\u5b83\u66f4\u6709\u53ef\u80fd\u63d0\u4f9b\u660e\u786e\u7684\u7ed3\u8bba\u3002\u4e0eELISA\u53ea\u80fd\u7ed9\u51fa\u201c\u662f\u201d\u6216\u201c\u5426\u201d\u7684\u7b54\u6848\u4e0d\u540c\uff0c\u86cb\u767d\u8d28\u5370\u8ff9\u8fd8\u80fd\u4ece\u590d\u6742\u6df7\u5408\u7269\u4e2d\u83b7\u5f97\u5173\u4e8e\u76ee\u6807\u86cb\u767d\u7684\u5927\u91cf\u4fe1\u606f\uff0c\u5305\u62ec\u5176\u5927\u5c0f\u548c\u4e30\u5ea6\u3002<br><br>\u4ee5\u4e0b\u662f\u5173\u4e8e\u5728\u4f55\u65f6\u4f7f\u7528\u6bcf\u79cd\u6280\u672f\u8fdb\u884c\u86cb\u767d\u8d28\u68c0\u6d4b\u548c\u5b9a\u91cf\u7684\u6307\u5357\u3002\n<\/p>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4f55\u65f6\u4f7f\u7528elisa\u8fdb\u884c\u86cb\u767d\u5206\u6790\"><strong>\u4f55\u65f6\u4f7f\u7528ELISA\u8fdb\u884c\u86cb\u767d\u5206\u6790<\/strong><\/h3>\n\n\n\n<p>\n\u4f5c\u4e3a\u6700\u7075\u654f\u7684\u514d\u75ab\u5206\u6790\u65b9\u6cd5\uff0cELISA\u51ed\u501f\u5176\u9ad8\u7075\u654f\u5ea6\u53ef\u7528\u4e8e\u68c0\u6d4b\u5c11\u91cf\u3001\u7a00\u6709\u53ca\u4f4e\u4e30\u5ea6\u76ee\u6807\u86cb\u767d\u3002\u5f53\u4f60\u9700\u8981\u7cbe\u786e\u6d4b\u5b9a\u86cb\u767d\u6d53\u5ea6\u65f6\uff0cELISA\u66f4\u4e3a\u5408\u9002\u3002\u800c\u86cb\u767d\u8d28\u5370\u8ff9\u53ea\u80fd\u6d4b\u91cf\u76f8\u5bf9\u86cb\u767d\u4e30\u5ea6\uff0c\u65e0\u6cd5\u83b7\u5f97\u7edd\u5bf9\u6d53\u5ea6\u3002<br><br>\n\u901f\u5ea6\u4e5f\u662f\u9009\u62e9ELISA\u800c\u975e\u86cb\u767d\u8d28\u5370\u8ff9\u7684\u53e6\u4e00\u4e2a\u539f\u56e0\u3002ELISA\u64cd\u4f5c\u6d41\u7a0b\u66f4\u5feb\uff0c\u5bf9\u6837\u54c1\u51c6\u5907\u8981\u6c42\u8f83\u4f4e\uff0c\u975e\u5e38\u9002\u5408\u65e5\u5e38\u68c0\u6d4b\u5b9e\u9a8c\u3002\u4e0e\u86cb\u767d\u8d28\u5370\u8ff9\u4e0d\u540c\uff0cELISA\u91c7\u7528\u5fae\u5b54\u677f\u548c\u8bfb\u677f\u4eea\uff0c\u53ef\u5b9e\u73b0\u5feb\u901f\u6837\u54c1\u5206\u6790\u3002\u200b<br><br>\n\u6b64\u5916\uff0cELISA\u8fc7\u7a0b\u4e5f\u66f4\u52a0\u7b80\u4fbf\u3002\u5b83\u4f7f\u7528\u8f83\u5c0f\u4f53\u79ef\u6837\u54c1\u548c\u6807\u51c696\u5b54\u677f\uff0c\u5e76\u652f\u6301\u591a\u91cd\u68c0\u6d4b\uff0c\u8fd8\u53ef\u4ee5\u5b9e\u73b0\u81ea\u52a8\u5316\uff0c\u4ece\u800c\u51cf\u5c11\u9519\u8bef\u548c\u91cd\u590d\u6027\u624b\u5de5\u64cd\u4f5c\u3002\u5982\u679c\u4f60\u9700\u8981\u9ad8\u901a\u91cf\u6216\u81ea\u52a8\u5316\u5b9e\u9a8c\u5ba4\u8bbe\u7f6e\uff0c\u53ef\u4ee5\u9009\u62e9\u8fd9\u79cd\u65b9\u6cd5\u3002\u6b64\u5916\uff0c\u76f8\u6bd4\u6d89\u53ca\u51dd\u80f6\u5206\u79bb\u3001\u819c\u8f6c\u79fb\u3001\u6210\u50cf\u7b49\u590d\u6742\u6b65\u9aa4\u7684\u86cb\u767d\u8d28\u5370\u8ff9\uff0c\u5c06ELISA\u6559\u5b66\u4f20\u6388\u7ed9\u521d\u7ea7\u79d1\u7814\u4eba\u5458\u4f1a\u66f4\u5bb9\u6613\u4e00\u4e9b\u3002<br><br>\n\u5982\u679c\u4f60\u53ea\u9700\u8981\u77e5\u9053\u67d0\u79cd\u86cb\u767d\u662f\u5426\u5b58\u5728\uff0c\u4ee5\u53ca\u53ef\u80fd\u60f3\u8981\u68c0\u6d4b\u5176\u6d53\u5ea6\uff0c\u90a3\u4e48\u4ec5\u7528ELISA\u5c31\u8db3\u591f\u4e86\u3002\n<\/p>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4e86\u89e3\u5982\u4f55\u9009\u62e9\u5408\u9002\u7684elisa\u8bd5\u5242\u76d2\u7c7b\u578b-gt-gt\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/antibodies\/immunoassays\/elisa-kits.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogKits\">\u4e86\u89e3\u5982\u4f55\u9009\u62e9\u5408\u9002\u7684ELISA\u8bd5\u5242\u76d2\u7c7b\u578b<\/a> &gt;&gt;<\/h4>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u4f55\u65f6\u4f7f\u7528\u86cb\u767d\u8d28\u5370\u8ff9\u8fdb\u884c\u86cb\u767d\u5206\u6790\"><strong>\u4f55\u65f6\u4f7f\u7528\u86cb\u767d\u8d28\u5370\u8ff9\u8fdb\u884c\u86cb\u767d\u5206\u6790<\/strong><\/h3>\n\n\n\n<p>\nWestern\u5370\u8ff9\u6cd5\u6700\u9002\u5408\u4ece\u590d\u6742\u7684\u6df7\u5408\u7269\u6216\u6837\u672c\u4e2d\u68c0\u6d4b\u5404\u79cd\u86cb\u767d\u8d28\u3002\u4f7f\u7528Western\u5370\u8ff9\u6cd5\u7684\u53e6\u4e00\u4e2a\u5173\u952e\u539f\u56e0\u662f\u5b83\u5728\u6d4b\u91cf\u86cb\u767d\u8d28\u65f6\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\uff0c\u5373\u4f7f\u5728\u76f8\u5bf9\u8f83\u4f4e\u7684\u6c34\u5e73\u4e0b\u4e5f\u80fd\u68c0\u6d4b\u5230\u3002<br><br>\n\u5047\u5982\u4f60\u9700\u8981\u5173\u4e8e\u86cb\u767d\u8d28\u7684\u66f4\u591a\u4fe1\u606f\uff0c\u6bd4\u5982\u5206\u5b50\u91cf\u3001\u7eaf\u5ea6\u3001\u86cb\u767d-\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7ec6\u8282\u3001\u86cb\u767d\u8868\u8fbe\u53d8\u5316\u6216\u7ffb\u8bd1\u540e\u4fee\u9970\u7b49\uff0cWestern\u5370\u8ff9\u6cd5\u53ef\u4ee5\u6210\u4e3a\u4e00\u4e2a\u5f3a\u6709\u529b\u7684\u5de5\u5177\u3002<br><br>\n\u5982\u679c\u4f60\u5bf9\u7ed3\u5408\u6297\u4f53\u7684\u7279\u5f02\u6027\u6709\u4fe1\u5fc3\uff0c\u5e76\u4e14\u786e\u4fe1\u5b83\u80fd\u591f\u68c0\u6d4b\u975e\u9776\u6807\u86cb\u767d\uff0cWestern\u5370\u8ff9\u6cd5\u4e5f\u662f\u4e00\u4e2a\u6781\u597d\u7684\u9009\u62e9\u3002\u7279\u522b\u662f\u8367\u5149Western\u68c0\u6d4b\uff0c\u53ef\u4ee5\u5b9e\u73b0\u591a\u91cd\u68c0\u6d4b\uff0c\u5728\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u5206\u6790\u591a\u79cd\u86cb\u767d\u8d28\u3002<br><br>\n\u6700\u540e\uff0cWestern\u5370\u8ff9\u6cd5\u901a\u5e38\u88ab\u7528\u4f5c\u8bb8\u591a\u5176\u4ed6\u8865\u5145\u6027\u86cb\u767d\u5206\u6790\u65b9\u6cd5\uff08\u5982ELISA\u6216\u8d28\u8c31\uff09\u7684\u786e\u8ba4\u5de5\u5177\uff0c,\u5c24\u5176\u662f\u5728\u4e34\u5e8a\u533b\u5b66\u8bca\u65ad\u4e2d\uff0c\u56e0\u4e3a\u5b83\u5bf9\u4e8e\u6392\u9664\u5047\u9633\u6027\u6216\u5047\u9634\u6027\u7ed3\u679c\u975e\u5e38\u53ef\u9760\u3002\u7ed3\u679c\u4e3a\u9634\u6027\u7684\u60c5\u51b5\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u6b22\u8fce\u67e5\u9605\u6211\u4eec\u7684-2024\u5b9a\u91cfwestern\u5370\u8ff9\u53d1\u8868\u6307\u5357-gt-gt\"><a href=\"https:\/\/www.thermofisher.com\/blog\/life-in-the-lab\/2023-guide-to-quantitative-western-blot-publication\/?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWBGuid\">\u6b22\u8fce\u67e5\u9605\u6211\u4eec\u7684\u300a2024\u5b9a\u91cfWestern\u5370\u8ff9\u53d1\u8868\u6307\u5357\u300b\u3002<\/a> &gt;&gt;<\/h4>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u66f4\u591a\u5de5\u5177\u4e0e\u8d44\u6e90\"><strong>\u66f4\u591a\u5de5\u5177\u4e0e\u8d44\u6e90<\/strong><\/h3>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a-elisa\"><strong>\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff08ELISA\uff09 <\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogEHub\">ELISA\u4ea7\u54c1\u53ca\u914d\u4ef6\u4e00\u7ad9\u5f0f\u6307\u5357<\/a>\uf0b7\u5305\u62ec\u5373\u7528\u578b\u8bd5\u5242\u76d2\u3001\u5fae\u5b54\u677f\u548c\u8bfb\u677f\u4eea\u3001\u5851\u6599\u5668\u76bf\u3001\u5c01\u95ed\u7f13\u51b2\u6db2\u548c\u8bd5\u5242\u3001\u4eea\u5668\u8bbe\u5907\u53ca\u5176\u4ed6\u5668\u6750\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/lab-equipment\/microplate-instruments\/plate-readers.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogPltRdr\">\u5fae\u5b54\u677f\u8bfb\u677f\u4eea<\/a>\u9002\u7528\u4e8eELISA\u677f\u53ca\u66f4\u591a\u5e94\u7528<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/antibodies\/immunoassays\/elisa-kits.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogKits\">ELISA\u8bd5\u5242\u76d2\u53ca\u7ec4\u4ef6<\/a>\u5305\u542b\u9009\u62e9\u5408\u9002\u8bd5\u5242\u76d2\u7c7b\u578b\u7684\u4fe1\u606f<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-elisa.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogELISA\">ELISA\u6982\u8ff0<\/a>\u5305\u62ec\u5bf9ELISA\u68c0\u6d4b\u65b9\u6cd5\u7684\u8be6\u7ec6\u89e3\u91ca<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/elisabuilder\/?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogElsBld#!\/\">ELISA\u6784\u5efa\u5668<\/a>\u5e2e\u52a9\u60a8\u5f00\u53d1\u72ec\u7279\u68c0\u6d4b\u65b9\u6848\u7684\u5de5\u5177<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u86cb\u767d\u514d\u75ab\u5370\u8ff9-western-blot\"><strong>\u86cb\u767d\u514d\u75ab\u5370\u8ff9\uff08Western Blot\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstrn\">\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u4e2d\u5fc3<\/a>\u5305\u62ec\u5de5\u4f5c\u6d41\u7a0b\u4ea7\u54c1\u76ee\u5f55\u3001\u5b9e\u9a8c\u65b9\u6848\u3001\u914d\u65b9\u3001\u624b\u518c\u3001\u7f51\u7edc\u7814\u8ba8\u4f1a\u7b49\u5185\u5bb9<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/iwestern-workflow.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstWrk\">iWestern\u5de5\u4f5c\u6d41\u7a0b<\/a>\u7528\u4e8e\u7b80\u5316\u60a8\u7684\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u6d41\u7a0b\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/transfer-proteins-western-blot\/iblot-dry-blotting-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogiBlot\">iBlot 3\u86cb\u767d\u8f6c\u79fb\u7cfb\u7edf<\/a>\u7528\u4e8e\u86cb\u767d\u8f6c\u79fb<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/detect-proteins-western-blot\/western-blot-imaging-analysis\/ibright-systems.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogiBrit\">iBright \u6210\u50cf\u7cfb\u7edf<\/a>\u7528\u4e8e\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u5206\u6790<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-gel-electrophoresis-information.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogWstEdu\">\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u6559\u80b2\u4e2d\u5fc3<\/a>\u5305\u62ec\u64cd\u4f5c\u89c6\u9891\u3001\u6545\u969c\u6392\u9664\u6307\u5357\u548c\u8d44\u6599\u5e93\u8d44\u6e90<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u8fd8\u60f3\u4e86\u89e3\u66f4\u591a-\u63a2\u7d22\u86cb\u767d\u5206\u6790\u5b66\u4e60\u4e2d\u5fc3-thermofisher-com-proteinlearning-nbsp\"><strong>\u00bb \u8fd8\u60f3\u4e86\u89e3\u66f4\u591a\uff1f\u63a2\u7d22\u86cb\u767d\u5206\u6790\u5b66\u4e60\u4e2d\u5fc3\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogProtLC\">thermofisher.com\/proteinlearning<\/a><strong>&nbsp;<\/strong><\/h3>\n\n\n\n<p><em>\u60f3\u9605\u8bfb\u66f4\u591a\u7c7b\u4f3c\u7684\u6545\u4e8b\u5417\uff1f<\/em><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/connect-science-subscribe.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt10933_blg50608_0so_blg_il_awa_og_s00_C2SELISABlogC2SSub\"><em>\u8ba2\u9605 Connect to Science\uff08\u79d1\u5b66\u8fde\u7ebf\uff09<\/em><\/a><em>\uff0c\u60a8\u7684\u751f\u547d\u79d1\u5b66\u65b0\u95fb\u95e8\u6237\u3002<\/em><strong>##<\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading glfh_headerContainer\" id=\"h-\u53c2\u8003\u6587\u732e\"><strong>\u53c2\u8003\u6587\u732e<\/strong><\/h3>\n\n\n\n<p>\nSakamoto S\u3001Putalun W\u3001Vimolmangkang S \u7b49\u3002\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\u6cd5\u7528\u4e8e\u690d\u7269\u6b21\u7ea7\u4ee3\u8c22\u4ea7\u7269\u7684\u5b9a\u91cf\/\u5b9a\u6027\u5206\u6790 [\u66f4\u6b63\u5df2\u53d1\u8868\u5728 J Nat Med. 2018\u5e741\u67085\u65e5\uff1b:]\u3002(\u81ea\u7136\u533b\u5b66\u6742\u5fd7). 2018;72(1):32-42. doi:10.1007\/s11418-017-1144-z<br><br>\n\n\u65e0\u65e5\u671f)\u3002\u82f1\u56fd\u514d\u75ab\u5b66\u4f1a\u3002\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\u6cd5\uff08ELISA\uff09\u3002\u68c0\u7d22\u81ea<a href=\"https:\/\/www.immunology.org\/public-information\/bitesized-immunology\/experimental-techniques\/enzyme-linked-immunosorbent-assay\">https:\/\/www.immunology.org\/public-information\/bitesized-immunology\/experimental-techniques\/enzyme-linked-immunosorbent-assay<\/a><br><br>\n\nAlhajj M\u3001Zubair M\u3001Farhana A\u3002\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\u6cd5\u3002[\u66f4\u65b0\u4e8e2023\u5e744\u670823\u65e5]\u3002\u6536\u5f55\u4e8e\uff1aStatPearls [\u4e92\u8054\u7f51]\u3002Treasure Island (FL)\uff1aStatPearls Publishing\uff1b2023\u5e741\u6708-\u3002\u53ef\u83b7\u53d6\u81ea\uff1a<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK555922\/\">https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK555922\/<\/a><br><br>\n\nSakamoto S\u3001Putalun W\u3001Vimolmangkang S \u7b49\u3002\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\u6cd5\u7528\u4e8e\u690d\u7269\u6b21\u7ea7\u4ee3\u8c22\u4ea7\u7269\u7684\u5b9a\u91cf\/\u5b9a\u6027\u5206\u6790 [\u66f4\u6b63\u5df2\u53d1\u8868\u5728 J Nat Med. 2018\u5e741\u67085\u65e5\uff1b:]\u3002(\u81ea\u7136\u533b\u5b66\u6742\u5fd7). 2018;72(1):32-42. doi:10.1007\/s11418-017-1144-z<br><br>\n\nGhosh R, Gilda JE, Gomes AV. \u63d0\u9ad8\u5bf9\u86cb\u767d\u8d28\u5370\u8ff9\uff08Western Blot\uff09\u51c6\u786e\u6027\u4fe1\u5fc3\u7684\u5fc5\u8981\u6027\u53ca\u7b56\u7565\u3002\u4e13\u5bb6\u8bc4\u8bba\uff1a\u86cb\u767d\u8d28\u7ec4\u5b66. 2014;11(5):549-560. doi:10.1586\/14789450.2014.939635<br><br>\n\nTie L, Xiao H, Wu DL, Yang Y, Wang P. \u836f\u7406\u5b9e\u9a8c\u4e2d\u826f\u597d\u5b9e\u8df5\u7b80\u8981\u6307\u5357\uff1a\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\u3002\u836f\u7406\u5b66\u62a5\uff08Acta Pharmacol Sin\uff09. 2021;42(7):1015-1017. doi:10.1038\/s41401-020-00539-7<br><br>\n\n(2023). \u7f8e\u56fd\u56fd\u5bb6\u4eba\u7c7b\u57fa\u56e0\u7ec4\u7814\u7a76\u6240\u3002\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\u3002\u68c0\u7d22\u81ea<a href=\"https:\/\/www.genome.gov\/genetics-glossary\/Western-Blot\">https:\/\/www.genome.gov\/genetics-glossary\/Western-Blot<\/a><br><br>\n\nGilda JE, Ghosh R, Cheah JX, West TM, Bodine SC, Gomes AV. \u4f7f\u7528\u672a\u7ecf\u9a8c\u8bc1\u6297\u4f53\u8fdb\u884c\u86cb\u767d\u8d28\u5370\u8ff9\u7684\u8bef\u5dee\uff1a\u5efa\u7acb\u86cb\u767d\u8d28\u5370\u8ff9\u6700\u5c0f\u62a5\u544a\u6807\u51c6\uff08WBMRS\uff09\u7684\u5fc5\u8981\u6027\u3002PLoS One. 2015;10(8):e0135392. \u53d1\u8868\u65f6\u95f4\uff1a2015\u5e748\u670819\u65e5\u3002doi:10.1371\/journal.pone.0135392<br><br>\n\nBegum H, Murugesan P, Tangutur AD. \u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\uff1a\u79d1\u5b66\u4e0e\u751f\u7269\u533b\u5b66\u7814\u7a76\u4e2d\u7684\u5f3a\u5927\u5de5\u5177\u3002Biotechniques. 2022;73(1):58-69. doi:10.2144\/btn-2022-0003\n\n<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<p><em>\u00a9 2024 Thermo Fisher Scientific Inc. \u4fdd\u7559\u6240\u6709\u6743\u5229\u3002\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u6240\u6709\u5546\u6807\u5747\u4e3aThermo Fisher Scientific\u53ca\u5176\u5b50\u516c\u53f8\u7684\u8d22\u4ea7\u3002<\/em><br><br><em>\u4ec5\u9650\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u5f97\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" 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