{"id":256,"date":"2026-02-10T03:28:14","date_gmt":"2026-02-10T03:28:14","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=256"},"modified":"2026-03-10T06:20:25","modified_gmt":"2026-03-10T06:20:25","slug":"14-tips-for-a-successful-rna-extraction","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/14-tips-for-a-successful-rna-extraction\/","title":{"rendered":"\u6210\u529f\u63d0\u53d6RNA\u768414\u4e2a\u6280\u5de7"},"content":{"rendered":"\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"603\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1.jpg\" alt=\"\" class=\"wp-image-305\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1.jpg 1920w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1-300x94.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1-1024x322.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1-768x241.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/snip201-1-1536x482.jpg 1536w\" sizes=\"auto, (max-width: 1920px) 100vw, 1920px\" \/><\/figure>\n\n\n\n<p>\u5373\u4fbf\u662f\u7ecf\u9a8c\u6700\u4e30\u5bcc\u7684\u5206\u5b50\u751f\u7269\u5b66\u5bb6\uff0c\u5728RNA\u63d0\u53d6\u8fc7\u7a0b\u4e2d\u4e5f\u96be\u514d\u4f1a\u6709\u60c5\u7eea\u5931\u63a7\u7684\u65f6\u523b\u3002\u5728\u5916\u4eba\u770b\u6765\uff0c\u8fd9\u4f4d\u53ef\u601c\u7684\u79d1\u7814\u4eba\u5458\u4eff\u4f5b\u6b63\u5bf9\u7740\u4e00\u4e2a\u88c5\u6ee1\u900f\u660e\u6db2\u4f53\u7684\u666e\u901a\u8bd5\u7ba1\u5927\u53d1\u96f7\u9706\uff0c\u663e\u5f97\u9887\u4e3a\u5931\u6001\u3002<\/p>\n\n\n\n<p>\u7136\u800c\uff0c\u5bf9\u4e8e\u90a3\u4e9b\u4eb2\u5386\u8fc7RNA\u63d0\u53d6\u79cd\u79cd\u6ce2\u6298\u7684\u79d1\u7814\u5de5\u4f5c\u8005\u800c\u8a00\uff0c\u8fd9\u79cd\u60c5\u7eea\u5ba3\u6cc4\uff08\u88ab\u620f\u79f0\u4e3a\u201cRNA\u6012\u201d\uff09\u5374\u6781\u6613\u5f15\u53d1\u5171\u9e23\u3002\u4e8b\u5b9e\u4e0a\uff0c\u56e0RNA\u5b9e\u9a8c\u800c\u60c5\u7eea\u5931\u63a7\u662f\u4e00\u79cd\u81ea\u7136\u800c\u666e\u904d\u7684\u73b0\u8c61\u2014\u2014\u6bd5\u7adf\uff0c\u5373\u4fbf\u662f\u8d44\u6df1\u4e13\u5bb6\uff0c\u4e5f\u96be\u9003\u63d0\u53d6\u6548\u7387\u4f4e\u4e0b\u6216RNA\u610f\u5916\u964d\u89e3\u7684\u7a98\u5883\u3002<\/p>\n\n\n\n<p><br> \u4f46\u5bf9\u4e8e\u90a3\u4e9b\u7ecf\u5386\u8fc7RNA\u63d0\u53d6\u5404\u79cd\u75db\u82e6\u7684\u79d1\u5b66\u5bb6\u6765\u8bf4\uff0c\u5f88\u5bb9\u6613\u4ea7\u751f\u5171\u9e23\u3002\u6124\u6012\u7684\u7206\u53d1\uff08\u4e5f\u88ab\u79f0\u4e3aRNA\u6124\u6012\uff09\u662f\u4ece\u4e8bRNA\u5de5\u4f5c\u65f6\u81ea\u7136\u4e14\u5e38\u89c1\u7684\u526f\u4f5c\u7528\u3002\u8fd9\u5f88\u5bb9\u6613\u7406\u89e3\uff1a\u4f4e\u63d0\u53d6\u4ea7\u91cf\u548c\u964d\u89e3\u7684RNA\u5373\u4f7f\u5bf9\u8d44\u6df1\u79d1\u5b66\u5bb6\u4e5f\u53ef\u80fd\u53d1\u751f\u3002<br><br> \u83b7\u5f97\u9ad8\u8d28\u91cf\u6837\u672c\u7c7b\u578b\u4e2d\u7684RNA\u5df2\u7ecf\u6210\u4e3a\u8bb8\u591a\u5b9e\u9a8c\u65b9\u6848\u4e2d\u7684\u57fa\u7840\u73af\u8282\uff0c\u5305\u62ec\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08NGS\uff09\u3001\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR(qPCR)\u3001Northern\u6742\u4ea4\u548ccDNA\u5408\u6210\uff0c\u8bb8\u591a\u79d1\u5b66\u5bb6\u90fd\u5728\u65e5\u5e38\u64cd\u4f5c\u4e2d\u8fdb\u884c\u3002\u5982\u679cRNA\u8d28\u91cf\u8f83\u4f4e\uff0c\u4f1a\u663e\u8457\u5f71\u54cd\u540e\u7eed\u5206\u6790\uff0c\u5bfc\u81f4\u65f6\u95f4\u548c\u91d1\u94b1\u635f\u5931\u3002<br><br> \u5176\u5b9e\u5e76\u4e0d\u4e00\u5b9a\u8981\u5982\u6b64\u3002\u8bb8\u591aRNA\u63d0\u53d6\u95ee\u9898\u90fd\u662f\u53ef\u4ee5\u907f\u514d\u548c\u89e3\u51b3\u7684\u3002\u4e3a\u4e86\u52a9\u529b\u4f60\u5728RNA\u63d0\u53d6\u4e4b\u8def\u4e0a\u53d6\u5f97\u80dc\u5229\uff0c\u6211\u4eec\u51c6\u5907\u4e8614\u6761\u5efa\u8bae\uff0c\u5e2e\u4f60\u5c06\u201cRNA\u6124\u6012\u201d\u8f6c\u5316\u4e3a\u201cRNA\u667a\u6167\u201d\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9009\u62e9\u5408\u9002\u7684rna\u7eaf\u5316\u8bd5\u5242\u76d2\">\u9009\u62e9\u5408\u9002\u7684RNA\u7eaf\u5316\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p>\u5e02\u9762\u4e0a\u6709\u5f88\u591a\u7528\u4e8eRNA\u63d0\u53d6\u7684\u8bd5\u5242\u76d2\u548c\u8bd5\u5242\u3002\u4f46\u67d0\u4e9b\u65b9\u6848\u6216\u5f62\u5f0f\u53ef\u80fd\u66f4\u9002\u5408\u4f60\u7684\u76ee\u6807\u3002\u5728\u9009\u62e9\u524d\u8bf7\u52a1\u5fc5\u5bf9\u6bcf\u79cd\u8bd5\u5242\u76d2\u8fdb\u884c\u4ed4\u7ec6\u7814\u7a76\u6216\u8bd5\u5242\uff0c\u5e76\u8003\u8651\u60a8\u5c06\u4ece\u54ea\u79cd\u6837\u672c\u4e2d\u63d0\u53d6RNA\u3001\u8be5\u6837\u672c\u7684\u8d77\u59cb\u91cf\u3001\u4e0b\u6e38\u5206\u6790\u65b9\u6cd5\u3001\u6240\u9700\u901a\u91cf\u4ee5\u53ca\u60a8\u8981\u5206\u6790\u7684RNA\u7c7b\u578b\u3002<br><br> \u4f8b\u5982\uff0c\u5982\u679c\u60a8\u9700\u8981\u4e00\u79cd\u7528\u4e8e\u603bRNA\u9ad8\u901a\u91cf\u7eaf\u5316\u7684\u65b9\u6cd5\uff0c\u90a3\u4e48\u60a8\u53ef\u80fd\u4f1a\u8003\u8651\u53ef\u6269\u5c55\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/automated-purification-extraction\/automated-magmax-kits-nucleic-acid-extraction.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc3\" target=\"_blank\" rel=\"noopener\">MagMAX\u2122 \u8bd5\u5242\u76d2<\/a>\u800c\u4e0d\u662f<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/product-brand\/trizol.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc4\" target=\"_blank\" rel=\"noopener\">TRIzol RNA\u63d0\u53d6\u6cd5<\/a>\u3002\u4f46\u5982\u679c\u60a8\u7684\u6837\u672c\u6570\u91cf\u8f83\u5c11\uff0c\u5e76\u4e14\u5e0c\u671b\u91c7\u7528\u91d1\u6807\u51c6\u65b9\u6cd5\u4ee5\u83b7\u5f97\u9ad8\u8d28\u91cfRNA\uff0c\u60a8\u5f88\u53ef\u80fd\u6bcf\u6b21\u90fd\u4f1a\u9009\u62e9TRIzol\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6ce8\u610f\u8f93\u5165\u91cf\">\u6ce8\u610f\u8f93\u5165\u91cf<\/h2>\n\n\n\n<p>\n\u591a\u5e76\u4e0d\u603b\u662f\u66f4\u597d\u3002\u5927\u591a\u6570<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/rna-extraction.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc5\" target=\"_blank\" rel=\"noopener\">RNA\u5206\u79bb\u8bd5\u5242\u76d2<\/a>\u548c\u8bd5\u5242\u90fd\u4e3a\u7279\u5b9a\u6837\u672c\u7c7b\u578b\u63d0\u4f9b\u4e86\u63a8\u8350\u7684\u8d77\u59cb\u6750\u6599\u7528\u91cf\u3002\u6dfb\u52a0\u8d85\u8fc7\u63a8\u8350\u91cf\u53ef\u80fd\u4f1a\u6291\u5236\u6709\u6548\u88c2\u89e3\u6216\u963b\u788dRNA\u4e0e\u5176\u4ed6\u7ec6\u80de\u6210\u5206\u7684\u5206\u79bb\u3002\u5bf9\u4e8e\u67f1\u5f0f\u64cd\u4f5c\u6d41\u7a0b\uff0c\u60a8\u53ef\u80fd\u4f1a\u8d85\u8f7d\u67f1\u5b50\u7684\u7ed3\u5408\u80fd\u529b\uff0c\u4ece\u800c\u964d\u4f4e\u6574\u4f53\u4ea7\u7387\u3002<br><br>\n\n\u8bf7\u6ce8\u610f\uff0c\u4e0d\u540c\u52a8\u7269\u7ec4\u7ec7\u4e2d\u7684RNA\u542b\u91cf\u53ef\u80fd\u6709\u663e\u8457\u5dee\u5f02\uff1a\u5fc3\u810f\u548c\u808c\u8089\u7ec6\u80de\u7684\u603bRNA\u542b\u91cf\u4f4e\u4e8e\u5bcc\u542b\u6838\u9178\u7684\u813e\u810f\u548c\u80f8\u817a\u7ec6\u80de\u3002\u8fd9\u4e9b\u7ec4\u7ec7\u8fd8\u53ef\u80fd\u5f15\u5165<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/references\/ambion-tech-support\/rna-isolation\/tech-notes\/isolation-of-total-rna-from-difficult-tissues.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc6\" target=\"_blank\" rel=\"noopener\">\u989d\u5916\u7684\u5e72\u6270\u56e0\u7d20<\/a>\u8fd9\u4e9b\u56e0\u7d20\u53ef\u80fd\u4f1a\u4f7f\u63d0\u53d6\u8fc7\u7a0b\u53d8\u5f97\u590d\u6742\uff0c\u56e0\u6b64\u53ef\u80fd\u9700\u8981\u5bf9\u64cd\u4f5c\u6d41\u7a0b\u8fdb\u884c\u8c03\u6574\uff0c\u4ee5\u83b7\u5f97\u9ad8\u8d28\u91cf\u7684RNA\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u521b\u5efa\u65e0rna\u9176\u73af\u5883\">\u521b\u5efa\u65e0RNA\u9176\u73af\u5883<\/h2>\n\n\n\n<p>\nRNA\u9176\uff1f\u4f60\u4ece\u6ca1\u542c\u8bf4\u8fc7\u5427\uff1f<br><br>\n\n\u5b83\u4eec\u662f\u4f60\u63d0\u53d6RNA\u65f6\u6700\u5927\u7684\u654c\u4eba\u3002\u5b83\u4eec\u5f88\u96be\u88ab\u706d\u6d3b\u6216\u53bb\u9664\u3002RNase A\u5bb6\u65cf\u4e2d\u7684RNA\u9176\u901a\u8fc7\u591a\u4e2a\u4e8c\u786b\u952e\u7a33\u5b9a\uff0c\u5373\u4f7f\u5728\u53d8\u6027\u540e\uff0c\u4ecd\u80fd\u4fdd\u6301\u6d3b\u6027\uff0c<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10632727\/\">\u5373\u4f7f\u5df2\u7ecf\u53d8\u6027\u540e\u4e5f\u80fd\u4fdd\u6301\u6d3b\u6027<\/a>\u3002<sup>1,2<\/sup><br><br>\n\n\u4e3a\u4e86\u51cf\u5c11\u5728\u63d0\u53d6RNA\u8fc7\u7a0b\u4e2d\u610f\u5916\u5f15\u5165RNA\u9176\uff0c\u8bf7\u5efa\u7acb\u4e00\u4e2a\u65e0RNA\u9176\u7684\u5b9e\u9a8c\u53f0\u4e13\u7528\u533a\u57df\u3002\u8fd9\u4e2a\u533a\u57df\u53ef\u4ee5\u662f\u4f60\u7684\u5b9e\u9a8c\u53f0\u7684\u4e00\u90e8\u5206\uff0c\u7528\u80f6\u5e26\u6807\u8bb0\uff0c\u4e0eDNA\u6216\u86cb\u767d\u8d28\u63d0\u53d6\u533a\u5206\u5f00\u3002\u5728\u8fd9\u4e2a\u533a\u57df\u5185\uff0c\u4e13\u95e8\u5b58\u653e\u7528\u4e8eRNA\u63d0\u53d6\u5de5\u4f5c\u7684\u8bd5\u5242\u3001\u8017\u6750\u3001\u79fb\u6db2\u5668\u548c\u624b\u5957\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7a7f\u6234\u4e2a\u4eba\u9632\u62a4\u88c5\u5907\">\u7a7f\u6234\u4e2a\u4eba\u9632\u62a4\u88c5\u5907<\/h2>\n\n\n\n<p>\n\u4f60\u662fRNase\u7684\u4e3b\u8981\u6765\u6e90\u3002\u4f60\u7684<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19809422\/\">\u6c57\u6db2\u548c\u76ae\u80a4\u6cb9\u8102<\/a>\u662fRNase\u6c61\u67d3\u7684\u4e30\u5bcc\u6765\u6e90\u3002\u56e0\u6b64\uff0c\u6234\u624b\u5957\u4ee5\u9632\u6b62\u624b\u90e8\u6c61\u67d3\u81f3\u5173\u91cd\u8981\u3002\u5982\u679c\u4f60\u7528\u6b63\u5728\u4f69\u6234\u7684\u624b\u5957\u63a5\u89e6\u4e86\u53ef\u80fd\u542b\u6709RNase\u7684\u8868\u9762\uff08\u5982\u95e8\u628a\u624b\u3001\u4f60\u7684\u8138\u6216\u4f60\u5728\u4e0a\u8ff0\u521b\u5efa\u7684\u65e0RNA\u9176\u533a\u4e4b\u5916\u7684\u5176\u4ed6\u8868\u9762\uff09\uff0c\u8bf7\u52a1\u5fc5\u66f4\u6362\u624b\u5957\u3002\u4f60\u4e5f\u53ef\u4ee5\u7a7f\u4e0a\u5b9e\u9a8c\u670d\uff0c\u4ee5\u786e\u4fdd\u8eab\u4f53\u5176\u4ed6\u90e8\u4f4d\u4e0d\u4f1a\u63a5\u89e6\u5230\u6837\u54c1\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4f7f\u7528\u65e0rna\u9176\u8bd5\u5242\u548c\u8017\u6750\">\u4f7f\u7528\u65e0RNA\u9176\u8bd5\u5242\u548c\u8017\u6750<\/h2>\n\n\n\n<p>\n\u4e0e\u4e0a\u8ff0\u89c2\u70b9\u4e00\u81f4\uff0c\u4f60\u8fd8\u53ef\u4ee5\u8d2d\u4e70\u6e05\u6d01\u4ea7\u54c1\u3001\u8017\u6750\u53ca\u5176\u4ed6\u8bd5\u5242\uff0c\u4ee5\u5e2e\u52a9\u63a7\u5236RNase\uff0c\u786e\u4fdd\u5b83\u4eec\u4e0d\u4f1a\u9760\u8fd1\u4f60\u7684RNA\u3002\u8868\u9762\u53bb\u6c61\u6eb6\u6db2\u53ef\u4ee5\u5e2e\u52a9\u53bb\u9664\u73bb\u7483\u5668\u76bf\u3001\u5b9e\u9a8c\u53f0\u548c\u79fb\u6db2\u5668\u4e0a\u7684RNase\u3002<br><br>\n\u4f60\u53ef\u80fd\u8ba4\u4e3a\u6240\u6709\u7ba1\u5b50\u548c\u79fb\u6db2\u5668\u5438\u5934\u5728\u51fa\u5382\u65f6\u90fd\u662f\u65e0RNA\u9176\u7684\uff0c\u4f46\u8bb8\u591a\u4ea7\u54c1\u5728\u5236\u9020\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u5df2\u88ab\u5f15\u5165\u4e86RNase\u3002\u8bf7\u786e\u4fdd\u4f60\u7684\u8017\u6750\u7ecf\u8fc7\u65e0RNA\u9176\u8ba4\u8bc1\uff0c\u5e76\u4e14\u4f7f\u7528\u5e26\u8fc7\u6ee4\u82af\u7684\u5438\u5934\uff0c\u4ee5\u9632\u6b62\u79fb\u6db2\u5668\u5438\u5934\u6216\u6837\u54c1\u95f4\u4ea4\u53c9\u6c61\u67d3\u3002\n<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright\"><img loading=\"lazy\" decoding=\"async\" width=\"1707\" height=\"2560\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1.jpg\" alt=\"Trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results with qRT-PCR. Keep lab equipment and work surfaces RNase-free with RNaseZap and other specialty lab cleaning solutions. \" class=\"wp-image-304\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1.jpg 1707w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1-200x300.jpg 200w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1-683x1024.jpg 683w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1-768x1152.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1-1024x1536.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/col11209-am9780-10-inch-scaled-1-1366x2048.jpg 1366w\" sizes=\"auto, (max-width: 1707px) 100vw, 1707px\" \/><figcaption class=\"wp-element-caption\">RNaseZap\u548c\u5b9e\u9a8c\u5ba4\u6d17\u6da4\u5242\u6709\u52a9\u4e8e\u7ef4\u6301\u65e0RNA\u9176\u5b9e\u9a8c\u5ba4\u73af\u5883<\/figcaption><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8fdb\u884c\u9884\u5b9e\u9a8c-pilot-experiment\">\u8fdb\u884c\u9884\u5b9e\u9a8c\uff08Pilot Experiment\uff09<\/h2>\n\n\n\n<p>\n\u5728\u5f00\u59cb\u5b9e\u9a8c\u4e4b\u524d\uff0c\u4f60\u53ef\u80fd\u6ca1\u6709\u610f\u8bc6\u5230\u67d0\u4e2a\u6b65\u9aa4\u4f1a\u8ba9\u4f60\u7684RNA\u66b4\u9732\u4e8eRNase\u6c61\u67d3\uff0c\u6216\u8005\u4f60\u8ba1\u5212\u4f7f\u7528\u7684\u8bd5\u5242\u76d2\u5e76\u4e0d\u9002\u5408\u4f60\u7684\u6837\u672c\u7c7b\u578b\uff0c\u76f4\u5230\u4f60\u5f00\u59cb\u5b9e\u9645\u64cd\u4f5c\u65f6\u624d\u53d1\u73b0\u8fd9\u4e00\u70b9\u3002<span style=\"color: #339966\"><strong>RNA\u63d0\u53d6<\/strong><\/span>\u3002\u4e3a\u4e86\u9632\u6b62\u6d6a\u8d39\u60a8\u5b9d\u8d35\u7684\u6837\u672c\uff0c\u5efa\u8bae\u5148\u7528\u4ef7\u503c\u8f83\u4f4e\u7684\u6837\u672c\u8fdb\u884c\u9884\u5b9e\u9a8c\uff0c\u8fd9\u6837\u53ef\u4ee5\u4f18\u5316\u60a8\u7684RNA\u63d0\u53d6\u65b9\u6848\uff0c\u907f\u514d\u540e\u7eed\u56e0\u64cd\u4f5c\u4e0d\u5f53\u800c\u9020\u6210\u6602\u8d35\u7684\u8fd4\u5de5\u6216\u9700\u8981\u91cd\u65b0\u6536\u96c6\u6837\u672c\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7a33\u5b9a\u60a8\u7684rna\">\u7a33\u5b9a\u60a8\u7684RNA<\/h2>\n\n\n\n<p>RNA\u7684\u751f\u5316\u7279\u6027\u4f7f\u5176<a href=\"https:\/\/www.ebi.ac.uk\/training\/online\/courses\/biomacromolecular-structures\/rna\/\">\u672c\u8d28\u4e0a\u4e0d\u7a33\u5b9a<\/a>\uff0c\u5e76\u4e14\u67d0\u4e9bRNA\u5177\u6709<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19001483\/\">\u8f83\u77ed\u7684\u534a\u8870\u671f<\/a><sup>5,6<\/sup>\u3002\u4e3a\u4e86\u786e\u4fdd\u4e0d\u4f1a\u5bf9\u4e0b\u6e38\u5206\u6790\u4ea7\u751f\u504f\u5dee\uff0c\u60a8\u9700\u8981\u5728\u6837\u672c\u91c7\u96c6\u540e\u5c3d\u5feb\u7834\u574f\u6240\u6709\u5185\u6e90\u6027RNase\u3002\u60a8\u53ef\u4ee5\u901a\u8fc7\u4f7f\u7528TRIzol\u6216\u6211\u4eec\u7684\u88c2\u89e3\u7f13\u51b2\u6db2\u6765\u88c2\u89e3\u7ec6\u80de\uff0c\u4ece\u800c\u5b9e\u73b0\u8fd9\u4e00\u70b9\u3002<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/brands\/product-brand\/trizol.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc7\" target=\"_blank\" rel=\"noopener\">TRIzol<\/a>\u6216\u6765\u81ea\u6211\u4eec\u7684\u88c2\u89e3\u7f13\u51b2\u6db2<span style=\"color: #ff9900\"><a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/12183018A\" target=\"_blank\" rel=\"noreferrer noopener\"><span style=\"color: #ff9900\">PureLink\u2122 RNA Mini Kit<\/span><\/a>&nbsp;<\/span> \u5e76\u5c06\u6837\u672c\u51b7\u51bb\u5728-80\u00b0C\u4ee5\u4fbf\u540e\u7eed\u5904\u7406\u3002\u60a8\u4e5f\u53ef\u4ee5\u5728\u88c2\u89e3\u7f13\u51b2\u6db2\u91cd\u60ac\u540e\u7acb\u5373\u63d0\u53d6RNA\u3002\u6216\u8005\uff0c\u5c06\u6837\u672c\u5feb\u901f\u51b7\u51bb\u4e8e\u6db2\u6c2e\u4e2d\uff0c\u53ef\u4ee5\u9632\u6b62\u6837\u672c\u91c7\u96c6\u540eRNA\u7684\u964d\u89e3\u3002<br><br> \u5982\u679c\u60a8\u4e0d\u60f3\u5904\u7406\u6db2\u6c2e\u5e26\u6765\u7684\u9ebb\u70e6\uff0c\u53ef\u4ee5\u4f7f\u7528RNA\u7a33\u5b9a\u5242\uff0c\u4f8b\u5982<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/brands\/product-brand\/rnalater.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc8\" target=\"_blank\" rel=\"noopener\">RNAlater<\/a>\u3002\u5b83\u53ef\u4ee5\u7acb\u5373\u706d\u6d3bRNase\uff0c\u8ba9\u60a8\u80fd\u591f\u7075\u6d3b\u9009\u62e9\u5408\u9002\u7684\u65f6\u95f4\u63d0\u53d6RNA\uff0c\u800c\u65e0\u9700\u62c5\u5fc3RNA\u964d\u89e3\u95ee\u9898\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6dfb\u52a0\u673a\u68b0\u6216\u9176\u4fc3\u88c2\u89e3\u6b65\u9aa4\">\u6dfb\u52a0\u673a\u68b0\u6216\u9176\u4fc3\u88c2\u89e3\u6b65\u9aa4<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/references\/ambion-tech-support\/rna-isolation\/general-articles\/extraction-of-nucleic-acids-from-ffpe-samples.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc9\" target=\"_blank\" rel=\"noopener\">FFPE\u6837\u672c\uff08\u77f3\u8721\u5305\u57cb\u7ec4\u7ec7\uff09<\/a>\uff0c<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/references\/ambion-tech-support\/rna-isolation\/general-articles\/tips-for-working-with-blood-samples.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc10\" target=\"_blank\" rel=\"noopener\">\u8840\u6db2\u6837\u672c<\/a>\uff0c\u4ee5\u53ca\u67d0\u4e9b\u5fae\u751f\u7269\uff0c\u901a\u5e38\u5f88\u96be\u63d0\u53d6\u5230\u8db3\u591f\u91cf\u4e14\u9ad8\u8d28\u91cf\u7684RNA\uff0c\u56e0\u6b64\u53ef\u80fd\u9700\u8981\u989d\u5916\u6b65\u9aa4\u4ee5\u786e\u4fdd\u5b8c\u5168\u88c2\u89e3\u3002\u8fd9\u4e9b\u6b65\u9aa4\u5305\u62ec\u4f7f\u7528\u73e0\u78e8\u6cd5\u8fdb\u884c\u673a\u68b0\u88c2\u89e3\uff0c\u6216\u7528\u6eb6\u83cc\u9176\u3001\u86cb\u767d\u9176K\u8fdb\u884c\u9176\u9884\u5904\u7406\u3002\u5982\u524d\u6240\u8ff0\uff0c\u88c2\u89e3\u4e0d\u5b8c\u5168\u53ef\u80fd\u5bfc\u81f4\u57fa\u4e8e\u67f1\u7684\u63d0\u53d6\u65b9\u6cd5\u51fa\u73b0\u4e0b\u6e38\u95ee\u9898\u3002\u5728\u4ece\u96be\u4ee5\u5904\u7406\u7684\u6837\u54c1\u4e2d\u63d0\u53d6RNA\u4e4b\u524d\uff0c\u8bf7\u4ed4\u7ec6\u67e5\u9605\u64cd\u4f5c\u6d41\u7a0b\uff0c\u786e\u8ba4\u662f\u5426\u5305\u542b\u9488\u5bf9\u7279\u6b8a\u6837\u54c1\u7684\u5177\u4f53\u6b65\u9aa4\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4fdd\u6301\u4f4e\u6e29\">\u4fdd\u6301\u4f4e\u6e29<\/h2>\n\n\n\n<p>\nRNA\u7684\u4e0d\u7a33\u5b9a\u6027\u4f7f\u5f97\u5728\u6574\u4e2a\u5904\u7406\u8fc7\u7a0b\u4e2d\u4fdd\u6301\u6837\u54c1\u4f4e\u6e29\u53d8\u5f97\u81f3\u5173\u91cd\u8981\u3002\u5982\u679c\u4f60\u4ee5\u524d\u9047\u5230\u8fc7RNase\u76f8\u5173\u7684\u95ee\u9898\uff0c\u53ef\u4ee5\u8003\u8651\u8ba9\u63d0\u53d6\u6eb6\u6db2\u4fdd\u6301\u4f4e\u6e29\uff0c\u5728\u51b7\u5ba4\u4e2d\u8fdb\u884c\u63d0\u53d6\uff0c\u6216\u8005\u5728\u79bb\u5fc3\u6b65\u9aa4\u4e2d\u4f7f\u7528\u9884\u51b7\u7684\u79bb\u5fc3\u673a\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u53bb\u9664dna\u6c61\u67d3\">\u53bb\u9664DNA\u6c61\u67d3<\/h2>\n\n\n\n<p>\u5927\u591a\u6570RNA\u5206\u79bb\u8bd5\u5242\u76d2\u548c\u8bd5\u5242\u90fd\u80fd\u6709\u6548\u53bb\u9664\u6c61\u67d3\u7684\u57fa\u56e0\u7ec4\u6216\u8d28\u7c92DNA\u3002\u4f46\u67d0\u4e9b\u540e\u7eed\u5b9a\u91cf\u65b9\u6cd5\uff0c\u5982<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/spectroscopy-elemental-isotope-analysis\/molecular-spectroscopy\/ultraviolet-visible-visible-spectrophotometry-uv-vis-vis\/uv-vis-vis-instruments\/nanodrop-microvolume-spectrophotometers.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc11\" target=\"_blank\" rel=\"noopener\">NanoDrop<\/a>\uff0c\u6216\u5e94\u7528\uff0c\u5982qPCR\uff0c\u5373\u4f7f\u662f\u6781\u5fae\u91cf\u7684DNA\u4e5f\u80fd\u88ab\u68c0\u6d4b\u5230\uff0c\u4ece\u800c\u4f7f\u5206\u6790\u53d8\u5f97\u590d\u6742\u3002<br><br> \u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\uff0c\u8bb8\u591a\u57fa\u4e8e\u67f1\u5b50\u7684\u7eaf\u5316\u65b9\u6cd5\u90fd\u5305\u542b\u4e86DNase I\uff0c\u4f8b\u5982<a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/cn\/zh\/12185010\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink\u2122 DNase Set\uff08PureLink\u2122 DNase\u5957\u88c5<\/a><a href=\"https:\/\/bit.ly\/3bHbrYO\" target=\"_blank\" rel=\"noopener\">\uff09<\/a>\uff0c\u53ef\u7528\u4e8e\u67f1\u4e0a\u5904\u7406\u3002\u4f60\u4e5f\u53ef\u4ee5\u5728\u63d0\u53d6\u540e\u4f7f\u7528DNase I\u8fdb\u884c\u6d88\u5316\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-rna\u5b9a\u91cf\u4e0e\u8d28\u91cf\u63a7\u5236\u68c0\u6d4b\">RNA\u5b9a\u91cf\u4e0e\u8d28\u91cf\u63a7\u5236\u68c0\u6d4b<\/h2>\n\n\n\n<p>\u6709\u51e0\u79cd\u65b9\u6cd5\u53ef\u4ee5\u5bf9\u7eaf\u5316\u540e\u7684RNA\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u5e76\u83b7\u5f97\u51c6\u786e\u7684\u5b9a\u91cf\u7ed3\u679c\u3002\u4f60\u53ef\u4ee5\u5229\u7528\u7d2b\u5916\u5438\u6536\u6cd5\uff0c\u5728260nm\u6ce2\u957f\u4e0b\u6d4b\u6838\u9178\u3001280nm\u6ce2\u957f\u4e0b\u6d4b\u86cb\u767d\u6c61\u67d3\u3001230nm\u6ce2\u957f\u4e0b\u6d4b\u5176\u4ed6\u6742\u8d28\uff08\u5982\u5f02\u786b\u6c30\u9178\u80cd\uff09\u3002\u901a\u8fc7\u89c2\u5bdfA260\/A280\u6bd4\u503c\uff08\u76ee\u6807\u8303\u56f41.8-2.2\uff09\u548cA260\/A230\u6bd4\u503c\uff08\u76ee\u6807&gt;1.7\uff09\uff0c\u53ef\u4ee5\u8bc4\u4f30RNA\u7684\u7eaf\u5ea6\u3002<br><br> \u8367\u5149\u8ba1\uff0c\u4f8b\u5982<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/spectroscopy-elemental-isotope-analysis\/molecular-spectroscopy\/fluorometers\/qubit.html\" target=\"_blank\" rel=\"noreferrer noopener\">Qubit\u8367\u5149\u8ba1<\/a>\uff0c\u4e5f\u53ef\u4ee5\u5b9a\u91cfRNA\uff0c\u5e76\u4e14\u6bd4\u57fa\u4e8e\u7d2b\u5916\u5438\u6536\u7684\u65b9\u6cd5\u66f4\u4e3a\u7075\u654f\u3002\u57fa\u4e8e\u5fae\u6d41\u63a7\u7684\u65b9\u6cd5\u540c\u6837\u6709\u52a9\u4e8eRNA\u7684\u5b9a\u91cf\u548c\u8d28\u91cf\u5224\u65ad\u3002\u6837\u54c1\u4f1a\u7528\u8367\u5149\u67d3\u6599\u6807\u8bb0\uff0c\u5e76\u901a\u8fc7\u51dd\u80f6\u77e9\u9635\u8fdb\u884c\u7535\u6cf3\u3002\u4e0d\u540c\u7684RNA\u79cd\u7c7b\u4f1a\u6839\u636e\u5927\u5c0f\u5728\u77e9\u9635\u4e2d\u8fc1\u79fb\uff0c\u901a\u8fc7\u89c2\u5bdf\u5b8c\u6574\u7684\u8367\u5149\u8f68\u8ff9\u53ef\u4ee5\u4f30\u7b97RNA\u7684\u5b8c\u6574\u6027\u3002\u8be5\u65b9\u6cd5\u80fd\u591f\u68c0\u6d4bDNA\u6c61\u67d3\u7269\u6216RNA\u964d\u89e3\u4ea7\u7269\uff0c\u5e76\u5c06\u5176\u8f6c\u5316\u4e3a\u4e00\u4e2a1\u523010\u7684RNA\u5b8c\u6574\u6027\u6570\u503c\uff08RIN\uff09\uff0c\u5176\u4e2d10\u4ee3\u8868\u6700\u4f73\u7684RNA\u5b8c\u6574\u6027\u3002<\/p>\n\n\n\n<h2 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href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/automated-purification-extraction.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc14\">KingFisher\u2122\u7cfb\u7edf<\/a>\u3002\u81ea\u52a8\u5316\u6709\u52a9\u4e8e\u907f\u514dRNA\u63d0\u53d6\u8fc7\u7a0b\u4e2d\u6700\u4e3b\u8981\u7684\u95ee\u9898\u4e4b\u4e00\u2014\u2014\u56e0\u4eba\u5de5\u64cd\u4f5c\u5bfc\u81f4\u7684RNase\u6c61\u67d3\u3002\u540c\u65f6\uff0c\u5b83\u8fd8\u80fd\u5b9e\u73b0\u53ef\u91cd\u590d\u6027\u7684RNA\u7eaf\u5316\uff0c\u5e76\u4e14\u76f8\u6bd4\u624b\u52a8\u6837\u54c1\u5236\u5907\uff0c\u80fd\u5c06\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u51cf\u5c1175%\u3002\n<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u8ba9\u4f60\u7684\u6bcf\u4e00\u6b65rna\u63d0\u53d6\u90fd\u5c3d\u5584\u5c3d\u7f8e\">\u8ba9\u4f60\u7684\u6bcf\u4e00\u6b65RNA\u63d0\u53d6\u90fd\u5c3d\u5584\u5c3d\u7f8e<\/h3>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Rock every step of your sample prep\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/PXUelr51Vo8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5bfb\u6c42\u5e2e\u52a9\">\u5bfb\u6c42\u5e2e\u52a9<\/h2>\n\n\n\n<p>\n\u6709\u4e9b\u95ee\u9898\u592a\u5927\uff0c\u65e0\u6cd5\u5355\u9760\u81ea\u5df1\u89e3\u51b3\u3002\u5982\u679c\u60a8\u5728RNA\u63d0\u53d6\u65b9\u9762\u9700\u8981\u989d\u5916\u7684\u652f\u6301\u548c\u5e2e\u52a9\uff0c\u8bf7\u8bbf\u95ee\u6211\u4eec\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/technical-resources\/technical-reference-library\/nucleic-acid-purification-analysis-support-center\/rna-sample-collection-protection-isolation-support.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc15\" target=\"_blank\" rel=\"noopener\">RNA\u6837\u672c\u6536\u96c6\u3001\u4fdd\u62a4\u4e0e\u5206\u79bb\u652f\u6301\u4e2d\u5fc3<\/a>\u3002\u8fd9\u91cc\u6709\u6307\u5357\u3001\u57fa\u7840\u6559\u7a0b\u3001\u6545\u969c\u6392\u9664\u6280\u5de7\u3001\u57f9\u8bad\u4ee5\u53ca\u66f4\u591a\u8d44\u6e90\uff0c\u5e2e\u52a9\u60a8\u987a\u5229\u5b8c\u6210RNA\u63d0\u53d6\u3002<br><br>\n\n\u4e3a\u4e86\u8ba9\u60a8\u7ee7\u7eed\u5b66\u4e60\uff0c\u6211\u4eec\u8fd8\u63d0\u4f9b\u4e86\u5176\u4ed6\u5728\u7ebf\u8d44\u6e90\uff1a\n<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/brochures\/rna-isolation-purification-brochure.pdf?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc16\" target=\"_blank\" rel=\"noopener\">RNA\u53c2\u8003\u6307\u5357<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/ambion-tech-support\/rna-isolation\/general-articles\/the-do-s-and-don-ts-of-total-rna-isolation.html?icid=bid_sap_aut_r01_co_cp1434_pjt8107_col017423_0so_blg_op_awa_kt_s00_RNAextrc1\" target=\"_blank\" rel=\"noopener\">RNA\u5206\u79bb\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879\u4e0e\u7981\u5fcc\u4e8b\u9879<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/BID\/Application-Notes\/rna-extraction-purelink-kit-vs-qiagen-kit-app-note.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink\u8bd5\u5242\u76d2\u4e0eQiagen\u8bd5\u5242\u76d2\u5e94\u7528\u8bf4\u660e\u4e66<\/a><\/li>\n<\/ul>\n\n\n\n<p><strong><em>\u672c\u6587\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\uff0c\u4e0d\u9002\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/strong><br><br>\n\n___________________________________________________________________________________________________\n\n&nbsp;<br>\n\n<strong>\u53c2\u8003\u6587\u732e<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>RNA\u64cd\u4f5c\u57fa\u7840\u3002Thermo Fisher Scientific \u7f51\u7ad9\uff1a<a href=\"https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FBID%2FTechnical-Notes%2Frnasezap-rnase-decontamination-solution-tech-note.pdf&amp;title=VGVjaCBOb3RlOiBSTmFzZVphcCBSTmFzZSBEZWNvbnRhbWluYXRpb24gU29sdXRpb24uIFdvcmtpbmcgd2l0aCBSTkE6IHRoZSBiYXNpY3M=\">https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FBID%2FTechnical-Notes%2Frnasezap-rnase-decontamination-solution-tech-note.pdf&amp;title=VGVjaCBOb3RlOiBSTmFzZVphcCBSTmFzZSBEZWNvbnRhbWluYXRpb24gU29sdXRpb24uIFdvcmtpbmcgd2l0aCBSTkE6IHRoZSBiYXNpY3M=<\/a>\u3002\u53d1\u8868\u4e8e2019\u5e745\u67082\u65e5\u3002\u8bbf\u95ee\u65e5\u671f\uff1a2021\u5e747\u670826\u65e5\u3002<\/li>\n\n\n\n<li>Klink TA, Woycechowsky KJ, Taylor KM, Raines RT. \u4e8c\u786b\u952e\u5bf9\u6838\u7cd6\u6838\u9178\u9176A\u6784\u8c61\u7a33\u5b9a\u6027\u548c\u50ac\u5316\u6d3b\u6027\u7684\u8d21\u732e\u3002\u6b27\u6d32\u751f\u7269\u5316\u5b66\u6742\u5fd7\uff0c2000\uff1b267:566-572\u3002<\/li>\n\n\n\n<li>Gupta SK, Haigh BJ, Griffin FJ, Wheeler TT. \u54fa\u4e73\u52a8\u7269\u5206\u6ccc\u578bRNases\uff1a\u5bbf\u4e3b\u9632\u5fa1\u4e2d\u7684\u4f5c\u7528\u673a\u5236\u3002\u300a\u5148\u5929\u514d\u75ab\u300b\uff0c2013\uff1b19:86-97\u3002<\/li>\n\n\n\n<li>4.Zasloff M. \u4eba\u4f53\u76ae\u80a4\u7684\u6297\u83ccRNases\u3002\u300a\u76ae\u80a4\u75c5\u5b66\u7814\u7a76\u6742\u5fd7\u300b\uff0c2009\uff1b129:2091-2093\u3002<\/li>\n\n\n\n<li>RNA\u3002EMBL-EBI \u7f51\u7ad9\uff1a<a href=\"https:\/\/www.ebi.ac.uk\/training\/online\/courses\/biomacromolecular-structures\/rna\/\">https:\/\/www.ebi.ac.uk\/training\/online\/courses\/biomacromolecular-structures\/rna\/<\/a>\u3002\u8bbf\u95ee\u65e5\u671f\uff1a2021\u5e747\u670828\u65e5\u3002<\/li>\n\n\n\n<li><em>6.Sharova LV\u3001Sharov AA\u3001Nedorezov T\u3001Piao Y\u3001Shaik N\u3001Ko MS\u3002\u901a\u8fc7\u5bf9\u591a\u80fd\u6027\u548c\u5206\u5316\u4e2d\u7684\u5c0f\u9f20\u80da\u80ce\u5e72\u7ec6\u80de\u8fdb\u884cDNA\u5fae\u9635\u5217\u5206\u6790\uff0c\u83b7\u5f97\u4e8619,977\u4e2a\u57fa\u56e0\u7684mRNA\u534a\u8870\u671f\u6570\u636e\u5e93\u3002DNA Res. 2009<\/em><\/li>\n<\/ol>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" 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