{"id":267,"date":"2026-02-11T02:52:03","date_gmt":"2026-02-11T02:52:03","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=267"},"modified":"2026-03-10T06:21:23","modified_gmt":"2026-03-10T06:21:23","slug":"plasmid-preparation-sizes-applications-in-scientific-research","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/plasmid-preparation-sizes-applications-in-scientific-research\/","title":{"rendered":"\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u89c4\u683c\uff1a\u79d1\u5b66\u7814\u7a76\u4e2d\u7684\u5e94\u7528"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92dna\">\u8d28\u7c92DNA<\/h1>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit1\">\u8d28\u7c92DNA<\/a>\u5728\u79d1\u5b66\u7814\u7a76\u4e2d\uff0c\u8d28\u7c92DNA\u56e0\u5176\u514b\u9686\u3001\u8f6c\u79fb\u548c\u64cd\u4f5c\u57fa\u56e0\u7684\u80fd\u529b\u800c\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002\u9009\u62e9\u5408\u9002\u7684\u8d28\u7c92\u5236\u5907\u89c4\u6a21\u5e94\u6839\u636e\u6240\u9700\u8d28\u7c92\u7684\u6570\u91cf\u548c\u8d28\u91cf\u6765\u51b3\u5b9a\u3002\u6211\u4eec\u5c06\u8ba8\u8bba\u56db\u79cd\u4e3b\u8981\u7684\u5236\u5907\u89c4\u6a21\uff0c\u5305\u62ec\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\uff08miniprep\uff09\u3001\u4e2d\u91cf\u63d0\u53d6\uff08midiprep\uff09\u3001\u5927\u91cf\u63d0\u53d6\uff08maxi prep\uff09\u3001\u8d85\u5927\u91cf\u63d0\u53d6\uff08megaprep\uff09\u4ee5\u53ca\u6781\u5927\u91cf\u63d0\u53d6\uff08gigaprep\uff09\u3002\u540c\u65f6\uff0c\u6211\u4eec\u8fd8\u5c06\u7b80\u8981\u4ecb\u7ecd\u5b83\u4eec\u5404\u81ea\u6700\u9002\u5408\u7684\u4e00\u4e9b\u5177\u4f53\u7814\u7a76\u5e94\u7528\u3002<br><br>\n\n<strong>\u4e94\u79cd\u8d28\u7c92\u8bd5\u5242\u76d2\u89c4\u683c\u4e3a\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-mini-prep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit2\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\uff08miniprep\uff09<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-midiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit3\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u7c92\u4e2d\u91cf\u63d0\u53d6\uff08midiprep\uff09<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-maxiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit4\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u7c92\u5927\u91cf\u63d0\u53d6\uff08maxi prep\uff09<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#mega?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit20\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u7c92\u8d85\u5927\u91cf\u63d0\u53d6\uff08megaprep\uff09<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#giga?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit5\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u7c92\u6781\u5927\u91cf\u63d0\u53d6\uff08gigaprep\uff09<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\">\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-mini-prep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit6\"><strong>\u8d28\u7c92DNA\u5c0f\u91cf\u63d0\u53d6<\/strong><\/a>\u8fd9\u4e9b\u8bd5\u5242\u76d2\u88ab\u5e7f\u6cdb\u7528\u4e8e\u5e38\u89c4\u7684\u8d28\u7c92DNA\u5206\u79bb\uff0c\u80fd\u591f\u5feb\u901f\u9ad8\u6548\u5730\u83b7\u5f97\u5c11\u91cfDNA\u3002\u4e0e\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u76f8\u5173\u7684\u79d1\u7814\u5e94\u7528\u5305\u62ec\uff1a\n<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u6587\u5e93\u7b5b\u9009\uff1a\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u975e\u5e38\u9002\u5408\u4ece\u5927\u578bDNA\u6587\u5e93\u4e2d\u5feb\u901f\u5206\u79bb\u8d28\u7c92DNA\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u83b7\u5f97\u7b5b\u9009\u6240\u9700\u7684DNA\uff0c\u5e76\u8bc6\u522b\u611f\u5174\u8da3\u7684\u7279\u5b9a\u514b\u9686\uff0c\u4ece\u800c\u4fc3\u8fdb\u65b0\u57fa\u56e0\u6216\u9057\u4f20\u5143\u4ef6\u7684\u53d1\u73b0\u3002<\/li>\n\n\n\n<li>\u6d4b\u5e8f\uff1a\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u53ef\u4e3aSanger\u6d4b\u5e8f\u6216\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u63d0\u4f9b\u6a21\u677fDNA\u3002\u7814\u7a76\u4eba\u5458\u80fd\u591f\u83b7\u5f97\u8db3\u591f\u4e14\u9ad8\u8d28\u91cf\u7684DNA\u7528\u4e8e\u6d4b\u5e8f\u5e94\u7528\uff0c\u4ece\u800c\u5206\u6790\u57fa\u56e0\u5e8f\u5217\u3001\u9057\u4f20\u53d8\u5f02\u6216\u7a81\u53d8\u3002<\/li>\n\n\n\n<li>PCR\u6269\u589e\uff1a\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u5bf9\u4e8e\u5236\u5907PCR\u6269\u589e\u6240\u9700\u7684\u6a21\u677fDNA\u975e\u5e38\u6709\u4ef7\u503c\u3002\u8fd9\u5bf9\u4e8e\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u3001\u57fa\u56e0\u5206\u578b\u548c\u5206\u5b50\u8bca\u65ad\u7b49\u5e94\u7528\u81f3\u5173\u91cd\u8981\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u8fc7\u591c\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u7ec6\u83cc\u4f53\u79ef\u4e0e\u4ea7\u91cf<br>\n\u57f9\u517b\u4f53\u79ef\uff1a1 mL \u2013 5 mL<br>\n\u8fd1\u4f3c\u4ea7\u91cf\uff1a\u9ad8\u8fbe40\u5fae\u514b\uff08\u00b5g\uff09\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e2d\u91cf\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\">\u4e2d\u91cf\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-midiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit7\"><strong>\u8d28\u7c92DNA\u4e2d\u91cf\u63d0\u53d6<\/strong><\/a>\u8fd9\u4e9b\u8bd5\u5242\u76d2\u5c5e\u4e8e\u4e2d\u7b49\u89c4\u6a21\u7684\u8d28\u7c92\u5236\u5907\uff0c\u80fd\u591f\u63d0\u4f9b\u8db3\u591f\u7684DNA\u7528\u4e8e\u5404\u79cd\u79d1\u7814\u5e94\u7528\u3002\u4e00\u4e9b\u4e0e\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u76f8\u5173\u7684\u5e38\u89c1\u5e94\u7528\u5305\u62ec\uff1a\n<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u8f6c\u67d3\u7814\u7a76\uff1a\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u4f7f\u7814\u7a76\u4eba\u5458\u80fd\u591f\u83b7\u5f97\u8db3\u591f\u6570\u91cf\u7684\u8d28\u7c92DNA\uff0c\u4ee5\u9ad8\u6548\u8f6c\u67d3\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u3002\u8fd9\u5bf9\u4e8e\u7814\u7a76\u57fa\u56e0\u529f\u80fd\u3001\u86cb\u767d\u8868\u8fbe\u53ca\u5176\u4ed6\u7ec6\u80de\u8fc7\u7a0b\u81f3\u5173\u91cd\u8981\u3002<\/li>\n\n\n\n<li>\u57fa\u56e0\u6572\u9664\u7814\u7a76\uff1a\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u53ef\u7528\u4e8e\u6784\u5efa\u6572\u9664\u8f7d\u4f53\uff0c\u8fd9\u5bf9\u4e8e\u9776\u5411\u57fa\u56e0\u7834\u574f\u5b9e\u9a8c\u975e\u5e38\u5173\u952e\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u83b7\u5f97\u6240\u9700\u6570\u91cf\u7684DNA\uff0c\u4ee5\u5f15\u5165\u7279\u5b9a\u7684\u57fa\u56e0\u4fee\u9970\u5e76\u7814\u7a76\u7531\u6b64\u4ea7\u751f\u7684\u8868\u578b\u53d8\u5316\u3002<\/li>\n\n\n\n<li>\u5206\u5b50\u514b\u9686\uff1a\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2\u6709\u52a9\u4e8e\u6269\u589eDNA\u7247\u6bb5\uff0c\u4ee5\u4fbf\u540e\u7eed\u514b\u9686\u5230\u66f4\u5927\u7684\u8f7d\u4f53\u4e2d\u3002\u8fd9\u5bf9\u4e8e\u6784\u5efa\u91cd\u7ec4DNA\u5206\u5b50\u548c\u751f\u6210\u5404\u79cd\u4e0b\u6e38\u5e94\u7528\u6240\u9700\u7684DNA\u7247\u6bb5\u6587\u5e93\u5c24\u5176\u6709\u7528\u3002<\/li>\n<\/ul>\n\n\n\n<p><em>\u8d28\u7c92\u4e2d\u91cf\u63d0\u53d6\u8fc7\u591c\u7ec6\u83cc\u4f53\u79ef\u4e0e\u4ea7\u91cf<\/em><br>\n\u57f9\u517b\u4f53\u79ef\uff1a25\u2013100 mL<br>\n\u5927\u81f4\u4ea7\u91cf\uff1a\u6700\u9ad8\u53ef\u8fbe400 \u00b5g<br>\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-maxiprep\u8bd5\u5242\u76d2\">Maxiprep\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-maxiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit8\"><strong>\u8d28\u7c92DNA Maxiprep<\/strong><\/a>\u8fd9\u4e9b\u8bd5\u5242\u76d2\u4e13\u4e3a\u5927\u89c4\u6a21\u8d28\u7c92DNA\u7eaf\u5316\u800c\u8bbe\u8ba1\uff0c\u53ef\u4e3a\u5173\u952e\u7684\u79d1\u7814\u5e94\u7528\u63d0\u4f9b\u9ad8\u4ea7\u91cf\u7684DNA\u3002\u7814\u7a76\u4eba\u5458\u901a\u5e38\u5c06Maxiprep\u8bd5\u5242\u76d2\u7528\u4e8e\u4ee5\u4e0b\u76ee\u7684\uff1a\n<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u57fa\u56e0\u6cbb\u7597\uff1aMaxiprep\u8bd5\u5242\u76d2\u80fd\u591f\u751f\u4ea7\u5927\u91cf\u7528\u4e8e\u57fa\u56e0\u9012\u9001\u7684\u6cbb\u7597\u6027\u8d28\u7c92\u3002\u8fd9\u4e9b\u8d28\u7c92\u53ef\u4ee5\u643a\u5e26\u6cbb\u7597\u6027\u57fa\u56e0\uff0c\u901a\u8fc7\u5411\u76ee\u6807\u7ec6\u80de\u5f15\u5165\u529f\u80fd\u6027\u57fa\u56e0\u6765\u6cbb\u7597\u9057\u4f20\u75be\u75c5\u3002<\/li>\n\n\n\n<li>\u75ab\u82d7\u5f00\u53d1\uff1aMaxiprep\u8bd5\u5242\u76d2\u4fc3\u8fdb\u4e86\u8d28\u7c92\u7684\u5927\u89c4\u6a21\u751f\u4ea7\uff0c\u8fd9\u5bf9\u4e8e\u75ab\u82d7\u8f7d\u4f53\u6784\u5efa\u81f3\u5173\u91cd\u8981\u3002\u79d1\u5b66\u5bb6\u53ef\u4ee5\u5236\u5907\u5927\u91cf\u8d28\u7c92\u4f5c\u4e3a\u75ab\u82d7\u6297\u539f\u7684\u8f7d\u4f53\uff0c\u6709\u52a9\u4e8e\u5f00\u53d1\u6709\u6548\u7684\u75ab\u82d7\u3002<\/li>\n\n\n\n<li>\u86cb\u767d\u8868\u8fbe\uff1aMaxiprep\u8bd5\u5242\u76d2\u5728\u83b7\u5f97\u9ad8\u4ea7\u91cf\u91cd\u7ec4\u86cb\u767d\u65b9\u9762\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u9002\u7528\u4e8e\u751f\u5316\u6216\u7ed3\u6784\u7814\u7a76\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5927\u91cf\u8868\u8fbe\u548c\u7eaf\u5316\u611f\u5174\u8da3\u7684\u86cb\u767d\uff0c\u4fbf\u4e8e\u8be6\u7ec6\u8868\u5f81\u548c\u529f\u80fd\u5206\u6790\u3002<\/li>\n<\/ul>\n\n\n\n<p><em>\u8d28\u7c92maxiprep\u8fc7\u591c\u7ec6\u83cc\u4f53\u79ef\u4e0e\u4ea7\u91cf<\/em><br>\n\u57f9\u517b\u4f53\u79ef\uff1a100\u2013500\u6beb\u5347<br>\n\u5927\u81f4\u4ea7\u91cf\uff1a\u6700\u9ad8\u53ef\u8fbe1.5\u6beb\u514b<br>\n\n<\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/automated-plasmid-purification.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit19\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2024\/06\/PG3022-PJT11444-COL122969-Violator-Cust-PlasmidDNA-Banner-Global-F.jpg\" alt=\"Learn about KingFisher PlasmidPro\" class=\"wp-image-3181\" \/><\/a><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-megaprep\u8bd5\u5242\u76d2\">Megaprep\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#mega?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit10\"><strong>\u8d28\u7c92DNA Megaprep\uff08\u5927\u5236\u5907\uff09<\/strong><\/a>\u8fd9\u4e9b\u8bd5\u5242\u76d2\u4e13\u4e3a\u63d0\u4f9b\u6700\u9ad8\u4ea7\u91cf\u7684\u8d28\u7c92DNA\u800c\u8bbe\u8ba1\u3002Megaprep\u8bd5\u5242\u76d2\u7528\u4e8e\u4e0e\u8d28\u7c92DNA gigaprep\uff08\u8d85\u5927\u5236\u5907\uff09\u7c7b\u4f3c\u7684\u5e94\u7528\uff0c\u4f46\u5904\u7406\u7684\u4f53\u79ef\u66f4\u5927\u3002\n<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5927\u89c4\u6a21\u751f\u4ea7\uff1aMegaprep\u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7814\u7a76\u5e94\u7528\u4e4b\u4e00\u662f\u4e3a\u5de5\u4e1a\u6216\u5546\u4e1a\u76ee\u7684\u751f\u6210\u5927\u91cf\u8d28\u7c92DNA\u3002\u751f\u7269\u6280\u672f\u3001\u5236\u836f\u548c\u519c\u4e1a\u7b49\u884c\u4e1a\u901a\u5e38\u9700\u8981\u5927\u91cf\u8d28\u7c92DNA\u6765\u751f\u4ea7\u836f\u7528\u86cb\u767d\u3001\u6297\u4f53\u3001\u75ab\u82d7\u548c\u5de5\u4e1a\u9176\u3002<\/li>\n\n\n\n<li>\u57fa\u56e0\u7ec4\u7f16\u8f91\uff1aMegaprep\u8bd5\u5242\u76d2\u5728\u5236\u5907\u5927\u91cfCRISPR-Cas9\u8d28\u7c92\u4ee5\u9ad8\u6548\u8fdb\u884c\u57fa\u56e0\u7ec4\u7f16\u8f91\u5b9e\u9a8c\u4e2d\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002\u968f\u7740CRISPR-Cas9\u6280\u672f\u7684\u53d1\u5c55\uff0c\u57fa\u56e0\u7ec4\u7f16\u8f91\u5df2\u6210\u4e3a\u751f\u7269\u533b\u5b66\u7814\u7a76\u4e2d\u7684\u5f3a\u6709\u529b\u5de5\u5177\u3002Megaprep\u8bd5\u5242\u76d2\u4f7f\u7814\u7a76\u4eba\u5458\u80fd\u591f\u83b7\u5f97\u7f16\u7801CRISPR-Cas9\u7cfb\u7edf\u6240\u9700\u6570\u91cf\u7684\u8d28\u7c92DNA\uff0c\u4ece\u800c\u5b9e\u73b0\u5927\u89c4\u6a21\u7cbe\u786e\u7684\u57fa\u56e0\u4fee\u9970\u3002<\/li>\n\n\n\n<li>\u5408\u6210\u751f\u7269\u5b66\uff1aMegaprep\u8bd5\u5242\u76d2\u5bf9\u4e8e\u5408\u6210\u751f\u7269\u5b66\u5e94\u7528\u6240\u9700\u7684\u5927\u91cf\u8d28\u7c92DNA\u81f3\u5173\u91cd\u8981\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u83b7\u5f97\u5145\u8db3\u7684DNA\uff0c\u7528\u4e8e\u57fa\u56e0\u5de5\u7a0b\u548c\u57fa\u56e0\u64cd\u4f5c\u3002<\/li>\n<\/ol>\n\n\n\n<p>\n\u9009\u62e9\u5408\u9002\u7684\u8d28\u7c92\u5236\u5907\u89c4\u683c\u5bf9\u79d1\u5b66\u7814\u7a76\u6210\u529f\u81f3\u5173\u91cd\u8981\u3002Maxiprep\uff08\u5927\u578b\uff09\u3001Midiprep\uff08\u4e2d\u578b\uff09\u3001Miniprep\uff08\u5c0f\u578b\uff09\u548cGigaprep\uff08\u8d85\u5927\u578b\uff09\u8bd5\u5242\u76d2\u5404\u5177\u4f18\u52bf\uff0c\u5e76\u9488\u5bf9\u4e0d\u540c\u7814\u7a76\u9700\u6c42\u8bbe\u8ba1\u3002\u4e86\u89e3\u6bcf\u79cd\u89c4\u683c\u5bf9\u5e94\u7684\u5e94\u7528\uff0c\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u4f18\u5316\u5b9e\u9a8c\u6d41\u7a0b\uff0c\u83b7\u5f97\u6ee1\u8db3\u5404\u79cd\u79d1\u5b66\u63a2\u7d22\u6240\u9700\u6570\u91cf\u548c\u8d28\u91cf\u7684\u9ad8\u7eaf\u5ea6\u8d28\u7c92DNA\u3002<br><br>\n\u8981\u4e86\u89e3\u66f4\u591a\u4fe1\u606f\uff0c\u8bf7\u67e5\u770b\u5982\u4f55\u81ea\u52a8\u5316\u8d28\u7c92DNA\u7eaf\u5316\u6216\u63a2\u7d22\u4f17\u591a\u8d28\u7c92\u8f7d\u4f53\u53ef\u7528\u670d\u52a1\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-gigaprep\u8bd5\u5242\u76d2\">Gigaprep\u8bd5\u5242\u76d2<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#giga?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit9\"><strong>\u8d28\u7c92DNA Gigaprep\u8bd5\u5242\u76d2<\/strong><\/a>\u63d0\u4f9b\u5927\u89c4\u6a21\u8d28\u7c92DNA\u7eaf\u5316\u89e3\u51b3\u65b9\u6848\uff0c\u4e3a\u9ad8\u7ea7\u7814\u7a76\u5e94\u7528\u63d0\u4f9b\u5927\u91cfDNA\u3002\u4e00\u4e9b\u4e0eGigaprep\u8bd5\u5242\u76d2\u76f8\u5173\u7684\u91cd\u8981\u7814\u7a76\u5e94\u7528\u5305\u62ec\uff1a\n<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5927\u89c4\u6a21\u751f\u4ea7\uff1aGigaprep\u8bd5\u5242\u76d2\u7528\u4e8e\u4e3a\u5de5\u4e1a\u6216\u5546\u4e1a\u76ee\u7684\u751f\u6210\u5927\u91cf\u8d28\u7c92DNA\u3002\u8fd9\u5305\u62ec\u7528\u4e8e\u751f\u7269\u6280\u672f\u5e94\u7528\u7684\u5927\u89c4\u6a21\u8d28\u7c92\u751f\u4ea7\uff0c\u5982\u9176\u751f\u4ea7\u3001\u751f\u7269\u71c3\u6599\u5f00\u53d1\u6216\u5de5\u4e1a\u53d1\u9175\u8fc7\u7a0b\u3002<\/li>\n\n\n\n<li>\u57fa\u56e0\u7ec4\u7f16\u8f91\uff1aGigaprep\u8bd5\u5242\u76d2\u5bf9\u4e8e\u5236\u5907\u5927\u91cfCRISPR-Cas9\u8d28\u7c92\u81f3\u5173\u91cd\u8981\uff0c\u80fd\u591f\u9ad8\u6548\u5f00\u5c55\u57fa\u56e0\u7ec4\u7f16\u8f91\u5b9e\u9a8c\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u83b7\u5f97\u6240\u9700\u6570\u91cf\u7684\u8d28\u7c92DNA\uff0c\u4ee5\u5b9e\u73b0\u7cbe\u786e\u7684\u9057\u4f20\u4fee\u9970\uff0c\u4fc3\u8fdb\u57fa\u56e0\u529f\u80fd\u548c\u75be\u75c5\u673a\u5236\u7684\u7814\u7a76\u3002<\/li>\n\n\n\n<li>\u5408\u6210\u751f\u7269\u5b66\uff1aGigaprep\u8bd5\u5242\u76d2\u5728\u6784\u5efa\u590d\u6742\u7684\u57fa\u56e0\u56de\u8def\u548c\u4ee3\u8c22\u901a\u8def\u4e2d\u53d1\u6325\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u83b7\u5f97\u5927\u91cf\u8d28\u7c92DNA\uff0c\u7528\u4e8e\u521b\u5efa\u5408\u6210\u751f\u7269\u7cfb\u7edf\uff0c\u4ece\u800c\u4fc3\u8fdb\u65b0\u578b\u751f\u7269\u529f\u80fd\u7684\u8bbe\u8ba1\u4e0e\u5de5\u7a0b\u5316\u3002<\/li>\n<\/ul>\n\n\n\n<p><em>\u8d28\u7c92gigaprep\u8fc7\u591c\u5904\u7406\u7ec6\u83cc\u4f53\u79ef\u4e0e\u4ea7\u91cf<\/em><br>\n\u57f9\u517b\u4f53\u79ef\uff1a2.5\u5347 \u2013 5\u5347<br>\n\u5927\u81f4\u4ea7\u91cf\uff1a\u6700\u9ad8\u53ef\u8fbe15\u6beb\u514b\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92dna\u5236\u5907\u89c4\u683c\"><strong>\u8d28\u7c92DNA\u5236\u5907\u89c4\u683c<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>&nbsp;<\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-mini-prep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit12\"><strong>Miniprep\u5c0f\u91cf\u63d0\u53d6<\/strong><\/a><\/td><td><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-midiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit13\"><strong>Midiprep\u4e2d\u91cf\u63d0\u53d6<\/strong><\/a><\/td><td><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-maxiprep.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit14\"><strong>Maxiprep\u5927\u91cf\u63d0\u53d6<\/strong><\/a><\/td><td><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#mega?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit15\"><strong>Megaprep<\/strong><\/a><\/td><td><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/purelink-hipure-expi-plasmid-kits.html#giga?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit16\"><strong>Gigaprep<\/strong><\/a><\/td><\/tr><tr><td><strong>\u8fc7\u591c\u7ec6\u83cc\u57f9\u517b\u4f53\u79ef<\/strong><\/td><td>1\u20135 \u6beb\u5347<\/td><td>25\u2013100 \u6beb\u5347<\/td><td>100\u2013500 \u6beb\u5347<\/td><td>500 \u6beb\u5347\u20132.5 \u5347<\/td><td>2.5\u20135 \u5347<\/td><\/tr><tr><td><strong>Approximate yield<\/strong><\/td><td>\u6700\u9ad8\u53ef\u8fbe 40 \u5fae\u514b<\/td><td>\u6700\u9ad8400\u5fae\u514b<\/td><td>\u6700\u9ad81.5\u6beb\u514b<\/td><td>\u6700\u9ad85\u6beb\u514b<\/td><td>\u6700\u9ad815\u6beb\u514b<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u7eaf\u5ea6\u7b49\u7ea7\u548c\u5185\u6bd2\u7d20\u6c34\u5e73\"><strong>\n\u8d28\u7c92\u7eaf\u5ea6\u7b49\u7ea7\u548c\u5185\u6bd2\u7d20\u6c34\u5e73<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>\u7eaf\u5ea6\u7b49\u7ea7<\/strong><\/td><td><strong>\u5206\u5b50\u7ea7\u522b<\/strong><\/td><td><strong>\u8f6c\u67d3\u7ea7\u522b<\/strong><\/td><td><strong>\u9ad8\u7ea7\u8f6c\u67d3<\/strong><\/td><\/tr><tr><td>\u5185\u6bd2\u7d20\u6c34\u5e73<\/td><td>&gt;10 EU\/\u00b5g<\/td><td>0.1\u20131 EU\/\u00b5g<\/td><td>&lt; 0.1 EU\/\u00b5g<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/transfection-grade-dna.html?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit17\"><strong>\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u8d28\u7c92DNA\u7eaf\u5ea6\u7b49\u7ea7\u7684\u4fe1\u606f<\/strong><\/a><br><br>\n\n\u81ea\u52a8\u5316\u60a8\u7684\u8d28\u7c92DNA\u63d0\u53d6\uff0c\u4ee5\u8282\u7701\u65f6\u95f4\u3001\u63d0\u9ad8\u53ef\u9760\u6027\u5e76\u6269\u5927\u60a8\u7684\u7814\u7a76\u89c4\u6a21\u3002\u6b32\u4e86\u89e3\u66f4\u591a\u8be6\u60c5\uff0c\u8bf7\u8bbf\u95ee<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/kingfisher-plasmid-pro.html?icid=fl-bid-plasmidpro?icid=bid_sap_kgf_r01_co_cp1538_pjt10787_bidsapkgf1_0so_blg_op_awa_kt_siz_plasmidprokit18\" target=\"_new\" rel=\"noreferrer noopener\">KingFisher PlasmidPro \u4fe1\u606f\u9875\u9762<\/a>\u3002<br><br>\n\n<em><strong>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u5f97\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/strong><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u8d28\u7c92DNA 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