{"id":279,"date":"2026-02-11T08:40:16","date_gmt":"2026-02-11T08:40:16","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=279"},"modified":"2026-04-07T09:42:23","modified_gmt":"2026-04-07T09:42:23","slug":"understanding-ct-values","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/understanding-ct-values\/","title":{"rendered":"\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR(qPCR)\u4e2dCt\u503c\u7684\u7406\u89e3"},"content":{"rendered":"\n<p>\u4f5c\u8005\uff1aJohn Pfeifer<\/p>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u662f\u4e00\u79cd\u5728\u6bcf\u4e2aPCR\u5faa\u73af\u540e\u68c0\u6d4b\u7279\u5b9a\u57fa\u56e0\u5e8f\u5217\u6269\u589e\u7684\u6280\u672f\u3002\u8fd9\u9879\u6280\u672f\u53ef\u4ee5\u7528\u4e8e\u68c0\u6d4b\u6216\u5b9a\u91cf\u591a\u79cd\u5e94\u7528\u4e2d\u7684\u57fa\u56e0\u5e8f\u5217\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"666\" height=\"503\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/amplification20plot_svt05_p1s1_ldfa-3.jpg\" alt=\"A sample amplification plot \" class=\"wp-image-326\" title=\"A sample amplification plot\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/amplification20plot_svt05_p1s1_ldfa-3.jpg 666w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/amplification20plot_svt05_p1s1_ldfa-3-300x227.jpg 300w\" sizes=\"auto, (max-width: 666px) 100vw, 666px\" \/><\/figure>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u5206\u6790\u5305\u62ec\u4e00\u7ec4\u5be1\u6838\u82f7\u9178\uff0c\u7528\u4e8e\u6269\u589e\u548c\u68c0\u6d4b\u7279\u5b9a\u7684\u57fa\u56e0\u9776\u6807\u3002\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u5206\u6790\u901a\u5e38\u7531\u4e24\u4e2aPCR\u5f15\u7269\u548c\u4e00\u4e2a\u5e26\u6709\u8367\u5149\u6807\u8bb0\u7684\u63a2\u9488\u7ec4\u6210\uff0c\u8fd9\u79cd\u63a2\u9488\u88ab\u79f0\u4e3aTaqMan\u63a2\u9488\u3002<\/p>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6d4b\u8bd5\u6216\u5b9e\u9a8c\u901a\u5e38\u8bbe\u5b9a\u4e3a\u8fd0\u884c40\u4e2a\u5faa\u73af\uff0c\u4f46\u9ad8\u4ef7\u503c\u7684\u6570\u636e\u53ea\u6db5\u76d6\u5c11\u6570\u51e0\u4e2a\u5faa\u73af\uff0c\u5e76\u4e14\u8fd9\u4e9b\u5faa\u73af\u5728\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u53ef\u80fd\u6709\u6240\u4e0d\u540c\u3002\u5982\u4f55\u4ece\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6570\u636e\u4e2d\u7b5b\u9009\u51fa\u9ad8\u4ef7\u503c\u7684\u6570\u636e\u70b9\uff1f<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6269\u589e\u66f2\u7ebf\u56fe\">\u6269\u589e\u66f2\u7ebf\u56fe<\/h2>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6570\u636e\u901a\u5e38\u4ee5\u4e00\u79cd\u79f0\u4e3a\u6269\u589e\u66f2\u7ebf\u56fe\uff08\u89c1\u56fe1\uff09\u7684\u65b9\u5f0f\u8868\u793a\u3002\u7eb5\u8f74\u5355\u4f4d\u4e3a\u8367\u5149\u503c\uff0c\u6d4b\u91cf\u503c\u5e38\u88ab\u79f0\u4e3a\u0394Rn\uff0c\u5373\u7ecf\u8fc7\u57fa\u7ebf\u6263\u9664\u5e76\u6807\u51c6\u5316\u540e\u7684\u62a5\u544a\u4fe1\u53f7\u3002\u6a2a\u8f74\u5219\u8868\u793aPCR\u5faa\u73af\u6b21\u6570\u3002<\/p>\n\n\n\n<p>\u57fa\u7ebf\u5b9a\u4e49\u4e3a\u65e9\u671fPCR\u5faa\u73af\u4e2d\uff0c\u5728\u6269\u589e\u4fe1\u53f7\u5c1a\u672a\u53ef\u68c0\u6d4b\u5230\u4e4b\u524d\u7684\u6570\u636e\u3002\u5728\u7eb5\u8f74\u91c7\u7528\u7ebf\u6027\u523b\u5ea6\u7684\u6269\u589e\u66f2\u7ebf\u56fe\u4e2d\uff0c\u57fa\u7ebf\u5e94\u8868\u73b0\u4e3a\u4e00\u6761\u5e73\u76f4\u7684\u7ebf\u3002\u5728\u57fa\u7ebf\u5468\u671f\u5185\uff0c\u76ee\u6807\u57fa\u56e0\u53ef\u80fd\u5df2\u7ecf\u5f00\u59cb\u6269\u589e\uff0c\u4f46\u62a5\u544a\u4fe1\u53f7\uff08\u5982\u6765\u81eaTaqMan\u63a2\u9488\uff09\u5c1a\u672a\u79ef\u7d2f\u5230\u8db3\u4ee5\u88ab\u89c2\u6d4b\u5230\u7684\u7a0b\u5ea6\u3002\u5982\u679c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u5b58\u5728\u76ee\u6807\u5e8f\u5217\uff0c\u6700\u7ec8\u6269\u589e\u4fe1\u53f7\u5c06\u4f1a\u4ece\u57fa\u7ebf\u4e0a\u5347\u51fa\u6765\u3002<\/p>\n\n\n\n<p><b>\u56fe1<\/b><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"451\" height=\"215\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure201-8.jpg\" alt=\"A sample amplification plot\" class=\"wp-image-325\" title=\"Amplification plot\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure201-8.jpg 451w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure201-8-300x143.jpg 300w\" sizes=\"auto, (max-width: 451px) 100vw, 451px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u57fa\u56e0\u5b9a\u91cf\">\u57fa\u56e0\u5b9a\u91cf<\/h2>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u662f\u57fa\u56e0\u5b9a\u91cf\u7684\u91d1\u6807\u51c6\u3002\u5176\u539f\u56e0\u5728\u4e8e\u6307\u6570\u671f\u6269\u589e\u5177\u6709\u6781\u9ad8\u7684\u4e00\u81f4\u6027\u3002\u6307\u6570\u671f\uff08\u4e5f\u79f0\u4e3a\u51e0\u4f55\u671f\u3001\u5bf9\u6570\u671f\uff09\u662fPCR\u7684\u7b2c\u4e00\u4e2a\u9636\u6bb5\uff0c\u6b64\u65f6\u6240\u6709\u53cd\u5e94\u7269\u2014\u2014\u5982\u5f15\u7269\u3001DNA\u805a\u5408\u9176\u3001dNTP\u7b49\u2014\u2014\u90fd\u5904\u4e8e\u8fc7\u91cf\u72b6\u6001\uff0c\u4ece\u800c\u4fdd\u8bc1\u4e86\u6269\u589e\u6548\u7387\u7684\u4e00\u81f4\u6027\u3002\u8fd9\u79cd\u4e00\u81f4\u6027\u610f\u5473\u7740\uff0cPCR\u53cd\u5e94\u4e2d\u521d\u59cb\u76ee\u6807\u57fa\u56e0\u7684\u6570\u91cf\u5c06\u51b3\u5b9a\u6269\u589e\u4fe1\u53f7\u4f55\u65f6\u4ece\u57fa\u7ebf\u4e2d\u663e\u73b0\uff0c\u5982\u56fe2\u6240\u793a\u3002\u8d77\u59cb\u76ee\u6807\u6570\u91cf\u8d8a\u591a\uff0c\u6269\u589e\u4fe1\u53f7\u51fa\u73b0\u5f97\u5c31\u8d8a\u65e9\u3002<br><br> \u6bcf\u4e2a\u6269\u589e\u66f2\u7ebf\u9700\u8981\u4e00\u4e2a\u9ad8\u4ef7\u503c\u7684\u6570\u636e\u70b9\u6765\u8ba1\u7b97\u521d\u59cb\u76ee\u6807\u6570\u91cf\u503c\u3002\u57fa\u7ebf\u6570\u636e\u70b9\u6ca1\u6709\u5b9a\u91cf\u4ef7\u503c\u3002\u6307\u6570\u671f\u7684\u6570\u636e\u70b9\u4ef7\u503c\u6700\u5927\u3002\u5f53\u67d0\u4e00\u53cd\u5e94\u7269\u4e0d\u518d\u8db3\u4ee5\u7ef4\u6301\u4e00\u81f4\u6548\u7387\u65f6\uff0c\u6307\u6570\u671f\u7ed3\u675f\uff0c\u968f\u540e\u7684\u6570\u636e\u70b9\u4ef7\u503c\u964d\u4f4e\u3002\u56e0\u6b64\uff0c\u9700\u8981\u4e00\u79cd\u65b9\u6cd5\uff0c\u5728\u4e00\u81f4\u7684\u4f4d\u7f6e\u9009\u62e9\u6307\u6570\u671f\u6570\u636e\u70b9\uff0c\u4ee5\u4fbf\u5728\u540c\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u5177\u6709\u53ef\u6bd4\u6027\u3002<\/p>\n\n\n\n<p><strong>\u56fe2<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"374\" height=\"175\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure202-8.jpg\" alt=\"Amplification plot showing how target quantity affects signal emergence from baseline\" class=\"wp-image-324\" title=\"Amplification plot showing how target quantity affects signal emergence from baseline\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure202-8.jpg 374w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure202-8-300x140.jpg 300w\" sizes=\"auto, (max-width: 374px) 100vw, 374px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-ct\u503c\">Ct\u503c<\/h2>\n\n\n\n<p>\u4ece\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6269\u589e\u66f2\u7ebf\u83b7\u5f97\u7684\u5355\u4e2a\u6570\u636e\u70b9\u88ab\u79f0\u4e3a\u9608\u503c\u5faa\u73af\u6216\u201cCt\u201d\uff08\u4e5f\u79f0\u4e3aCq\uff09\u503c\u3002\u6700\u65e9\u4e14\u4ecd\u7136\u6700\u5e38\u7528\u7684Ct\u503c\u4ea7\u751f\u65b9\u6cd5\u88ab\u79f0\u4e3a\u57fa\u7ebf-\u9608\u503c\u6cd5\u3002<\/p>\n\n\n\n<p>\u5728\u6240\u6709\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u5316\u5b66\u4f53\u7cfb\u4e2d\uff0c\u603b\u4f1a\u5b58\u5728\u4e00\u5b9a\u7684\u57fa\u7ebf\u8367\u5149\uff0c\u5e76\u4e14\u4e0d\u540c\u5b54\u4e4b\u95f4\u53ef\u80fd\u7565\u6709\u5dee\u5f02\u3002\u901a\u8fc7\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u8f6f\u4ef6\u4e2d\u7684\u57fa\u7ebf\u6263\u9664\u7b97\u6cd5\uff0c\u8fd9\u79cd\u5dee\u5f02\u5927\u5927\u51cf\u5c11\uff0c\u8be5\u7b97\u6cd5\u5c06\u6240\u6709\u57fa\u7ebf\u8bbe\u5b9a\u4e3a\u7ea6\u96f6\u3002\u901a\u8fc7\u5c06\u6240\u6709\u57fa\u7ebf\u8bbe\u4e3a\u96f6\uff0c\u9608\u503c\u5c31\u80fd\u4ece\u7edf\u4e00\u7684\u8d77\u70b9\u8bbe\u7f6e\u3002<\/p>\n\n\n\n<p>\u9608\u503c\u662f\u9488\u5bf9\u67d0\u4e00\u5b9e\u9a8c\u9009\u5b9a\u7684\u8367\u5149\u503c\uff08\u0394Rn\uff09\uff0c\u7528\u4e8e\u8ba1\u7b97Ct\u503c\u3002\u9608\u503c\u901a\u5e38\u8bbe\u7f6e\u5728PCR\u7684\u6307\u6570\u671f\u5185\u3002\u5728\u653e\u5927\u66f2\u7ebf\u4e0a\uff0c\u7528\u5bf9\u6570\u5750\u6807y\u8f74\u6700\u5bb9\u6613\u8bc6\u522b\u51fa\u6307\u6570\u671f\uff0c\u6b64\u65f6\u5404\u6761\u66f2\u7ebf\u8868\u73b0\u4e3a\u659c\u7387\u76f8\u540c\u7684\u5e73\u884c\u76f4\u7ebf\uff08\u89c1\u56fe3\uff09\u3002\u5728\u6269\u589e\u66f2\u7ebf\u4e0a\uff0c\u9608\u503c\u8868\u73b0\u4e3a\u4e00\u6761\u6c34\u5e73\u76f4\u7ebf\u3002<\/p>\n\n\n\n<p>\u9608\u503c\u4e0e\u6269\u589e\u66f2\u7ebf\u76f8\u4ea4\u7684\u4f4d\u7f6e\u5373\u4ea7\u751fCt\u503c\u3002Ct\u503c\u901a\u5e38\u4e0d\u662f\u6574\u6570\u3002<\/p>\n\n\n\n<p><strong>\u56fe3<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"391\" height=\"207\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure203-8.jpg\" alt=\"Amplification plot showing exponential phases\" class=\"wp-image-327\" title=\"Amplification plot showing exponential phases\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure203-8.jpg 391w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure203-8-300x159.jpg 300w\" sizes=\"auto, (max-width: 391px) 100vw, 391px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5b9a\u6027\u7ed3\u679c\">\u5b9a\u6027\u7ed3\u679c<\/h2>\n\n\n\n<p>Ct\u503c\u53ef\u7528\u4e8e\u5b9a\u6027\u68c0\u6d4b\uff0c\u4f8b\u5982\u5224\u65ad\u75c5\u539f\u4f53\u7684\u5b58\u5728\u6216\u4e0d\u5b58\u5728\u3002\u6ca1\u6709Ct\u503c\u610f\u5473\u7740\u8be5\u53cd\u5e94\u4e2d\u672a\u68c0\u6d4b\u5230\u76ee\u6807\u7269\uff0c\u51fa\u73b0Ct\u503c\u5219\u8868\u793a\u76ee\u6807\u7269\u5b58\u5728\u3002<\/p>\n\n\n\n<p>\u5b9a\u6027\u68c0\u6d4b\u901a\u5e38\u5305\u542b\u4e00\u4e2a\u5bf9\u7167\u57fa\u56e0\uff0c\u7528\u4e8e\u8d28\u91cf\u63a7\u5236\u3002\u5982\u679c\u5bf9\u7167\u68c0\u6d4b\u7684Ct\u503c\u8fc7\u9ad8\uff0c\u5219\u76ee\u6807\u9634\u6027\u6837\u672c\u7ed3\u679c\u7684\u6709\u6548\u6027\u5c06\u53d7\u5230\u8d28\u7591\u3002\u4f8b\u5982\uff0c\u53ef\u80fd\u5728\u6837\u672c\u91c7\u96c6\u3001\u63d0\u53d6\u8fc7\u7a0b\u4e2d\u51fa\u73b0\u4e86\u95ee\u9898\uff0c\u6216\u8005\u5b58\u5728\u6291\u5236\u5242\u7b49\u60c5\u51b5\uff0c\u8fd9\u4e9b\u90fd\u53ef\u80fd\u5bfc\u81f4\u68c0\u6d4b\u7075\u654f\u5ea6\u4f4e\u4e8e\u53ef\u63a5\u53d7\u6c34\u5e73\u3002<\/p>\n\n\n\n<p>\u5bf9\u4e8e\u5b9a\u6027\u68c0\u6d4b\uff0c\u4e3a\u4e86\u4fdd\u8bc1\u6bcf\u6b21\u5b9e\u9a8c\u8fd0\u884c\u65f6Ct\u503c\u8ba1\u7b97\u7684\u4e00\u81f4\u6027\uff0c\u9608\u503c\u901a\u5e38\u662f\u56fa\u5b9a\u7684\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5b9a\u91cf\u7ed3\u679c\">\u5b9a\u91cf\u7ed3\u679c<\/h2>\n\n\n\n<p>\u5b9a\u91cf\u7ed3\u679c\u6709\u591a\u79cd\u7c7b\u578b\uff1a\u76f8\u5bf9\u4e0e\u7edd\u5bf9\uff0c\u4ee5\u53ca\u6570\u91cf\u4e0e\u6d53\u5ea6\u3002\u5728\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u8f6f\u4ef6\u4e2d\uff0c\u201cquantity\u201d\uff08\u6570\u91cf\uff09\u4e00\u8bcd\u88ab\u5e7f\u6cdb\u7528\u4e8e\u6db5\u76d6\u6240\u6709\u8fd9\u4e9b\u7ed3\u679c\u7c7b\u578b\u3002<\/p>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4e2d\u7684\u7edd\u5bf9\u5b9a\u91cf\u662f\u6307\u786e\u5b9a\u76ee\u6807\u5206\u5b50\u7684\u5b9e\u9645\u6570\u91cf\u6216\u6d53\u5ea6\uff1b\u800c\u76f8\u5bf9\u5b9a\u91cf\u5219\u662f\u7528\u975e\u5206\u5b50\u6216\u4efb\u610f\u5355\u4f4d\u6765\u786e\u5b9a\u6570\u91cf\u3002<\/p>\n\n\n\n<p>\u4e0d\u5efa\u8bae\u5c06Ct\u503c\u4f5c\u4e3a\u6700\u7ec8\u7684\u5b9a\u91cf\u7ed3\u679c\u8fdb\u884c\u62a5\u544a\uff0c\u56e0\u4e3aCt\u503c\u662f\u62bd\u8c61\u3001\u4e0d\u5b8c\u6574\u4e14\u6570\u5b66\u4e0a\u8f83\u4e3a\u590d\u6742\u7684\u6570\u636e\u3002\u4ec5\u6709Ct\u503c\u5728\u5b9a\u91cf\u4e0a\u662f\u4e0d\u5b8c\u6574\u7684\uff0c\u56e0\u4e3a\u5176\u5b9a\u91cf\u89e3\u91ca\u4f9d\u8d56\u4e8e\u6307\u6570\u9636\u6bb5\u6548\u7387\u3002\u4f8b\u5982\uff0cCt\u6807\u51c6\u5dee\u5e76\u4e0d\u50cf\u666e\u901a\u6807\u51c6\u5dee\u90a3\u6837\u8868\u73b0\u3002<\/p>\n\n\n\n<p>\u5b9a\u91cf\u7ed3\u679c\u5e94\u4ee5\u201c\u6570\u91cf\u201d\u5f62\u5f0f\u62a5\u544a\uff0c\u8fd9\u662f\u4e00\u79cd\u5b8c\u6574\u4e14\u6570\u5b66\u4e0a\u66f4\u7b80\u5355\u7684\u6570\u636e\u70b9\u3002\u201c\u6570\u91cf\u201d\u53ef\u4ee5\u901a\u8fc7Ct\u503c\u548c\u6307\u6570\u9636\u6bb5\u6548\u7387\u8ba1\u7b97\u5f97\u51fa\uff1a<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u516c\u5f0f1\">\u516c\u5f0f1<\/h3>\n\n\n\n<p class=\"has-text-align-center\">Quantity ~ e<sup>-Ct<\/sup> <\/p>\n\n\n\n<p class=\"has-text-align-left\">Where:<\/p>\n\n\n\n<p>\u6570\u91cf = \u521d\u59cb\u76ee\u6807\u57fa\u56e0\u6570\u91cf<\/p>\n\n\n\n<p>e = \u6307\u6570\u671f\u6548\u7387\uff0c\u7406\u60f3\u503c\u4e3a2\uff08\u76ee\u6807\u6bcf\u4e2a\u5faa\u73af\u7ffb\u500d\uff09<\/p>\n\n\n\n<p>Ct = \u9608\u503c\u5faa\u73af\u6570<\/p>\n\n\n\n<p>\u6d89\u53ca\u751f\u7269\u6837\u672c\u7684\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u5b9a\u91cf\u901a\u5e38\u5305\u62ec\u4e00\u4e2a\u6807\u51c6\u5316\u57fa\u56e0\u3002\u8be5\u57fa\u56e0\u7528\u4e8e\u6d4b\u91cf\u6837\u672c\u8d28\u91cf\uff0c\u5e76\u901a\u8fc7\u6837\u672c\u8d28\u91cf\u5bf9\u76ee\u6807\u91cf\u8fdb\u884c\u5f52\u4e00\u5316\uff0c\u4ee5\u83b7\u5f97\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\u7684\u76ee\u6807\u6d53\u5ea6\u3002<\/p>\n\n\n\n<p>\u6821\u51c6\u662f\u5b9a\u4e49\u6570\u636e\u96c6\u5355\u4f4d\u7684\u4e00\u79cd\u65b9\u6cd5\u3002\u5728\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4e2d\uff0c\u4e00\u4e2a\u5e38\u89c1\u505a\u6cd5\u662f\u4f7f\u7528\u67d0\u4e2a\u6837\u672c\u6216\u4e00\u7ec4\u6837\u672c\u7684\u5e73\u5747\u503c\u4f5c\u4e3a\u201c\u6821\u51c6\u5668\u201d\uff08\u4e5f\u79f0\u4e3a\u53c2\u8003\u6837\u672c\u6216\u53c2\u8003\u7ec4\uff09\u3002\u6821\u51c6\u5668\u6837\u672c\u6216\u7ec4\u7684\u76f8\u5bf9\u6570\u91cf\uff08RQ\uff09\u8bbe\u4e3a1\uff0c\u5176\u4ed6\u6240\u6709\u6837\u672c\u7684RQ\u503c\u90fd\u4ee5\u6b64\u4e3a\u53c2\u7167\u3002\u6821\u51c6\u5668\u53ef\u4ee5\u662f\u76f8\u5bf9\u7684\uff0c\u4e5f\u53ef\u4ee5\u662f\u7edd\u5bf9\u7684\u3002<\/p>\n\n\n\n<p>\u57281997\u5e74\u540d\u4e3a\u201c\u7528\u6237\u516c\u544a#2\u201d\u7684\u6587\u4ef6\u4e2d\uff0c\u65b9\u7a0b1\u88ab\u4fee\u6539\u7528\u4e8e\u6837\u672c\u8d28\u91cf\u5f52\u4e00\u5316\u548c\u6821\u51c6\uff0c\u5e76\u5c06\u6307\u6570\u6548\u7387\u8bbe\u5b9a\u4e3a100%\u3002\u7531\u6b64\u5f97\u5230\u7684\u516c\u5f0f\u88ab\u79f0\u4e3a\u6bd4\u8f83Ct\u6216\u0394\u0394Ct\u65b9\u6cd5\uff1a<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u65b9\u7a0b2\">\u65b9\u7a0b2<\/h3>\n\n\n\n<p class=\"has-text-align-center\">\u76f8\u5bf9\u6570\u91cf = 2<sup>-\u0394\u0394Ct<\/sup> <\/p>\n\n\n\n<p class=\"has-text-align-left\">Where:<\/p>\n\n\n\n<p>\u0394\u0394Ct \u662f\u901a\u8fc7\u6837\u54c1\u8d28\u91cf\u5f52\u4e00\u5316\u5e76\u6821\u51c6\u540e\u7684 Ct \u503c\u3002<\/p>\n\n\n\n<p>\u6ce8\u610f\uff0cCt \u503c\u7684\u5f52\u4e00\u5316\u662f\u901a\u8fc7\u51cf\u6cd5\u800c\u4e0d\u662f\u9664\u6cd5\u8fdb\u884c\u7684\uff0c\u7b26\u53f7 \u0394 \u8868\u793a\u51cf\u6cd5\u3002<\/p>\n\n\n\n<p>\u5bf9\u4e8e\u5b9a\u91cf\u68c0\u6d4b\uff0c\u53ef\u4ee5\u5728\u6307\u6570\u9636\u6bb5\u7684 \u0394Rn \u503c\u8303\u56f4\u5185\u8bbe\u7f6e\u9608\u503c\uff08\u89c1\u56fe4\uff09\u3002\u5728\u4ee5\u5bf9\u6570 y \u8f74\u663e\u793a\u7684\u6269\u589e\u66f2\u7ebf\u4e2d\uff0c\u6307\u6570\u9636\u6bb5\u7ed3\u675f\u4e8e\u66f2\u7ebf\u5f00\u59cb\u5411\u53f3\u5f2f\u66f2\u7684\u4f4d\u7f6e\u3002\u7531\u4e8e\u7cbe\u786e\u5ea6\u964d\u4f4e\uff0c\u9608\u503c\u4e0d\u5e94\u8bbe\u7f6e\u5728\u8be5\u5f2f\u66f2\u533a\u57df\u3002\u6b64\u5916\uff0c\u9608\u503c\u4e5f\u4e0d\u5e94\u8bbe\u7f6e\u5f97\u8fc7\u4f4e\uff0c\u5426\u5219\u6570\u636e\u4f1a\u8868\u73b0\u51fa\u66f4\u5927\u7684\u53d8\u5f02\u6027\u3002\u8fd9\u79cd\u53d8\u5f02\u6027\u662f\u7531\u4fe1\u566a\u6bd4\u4f4e\u9020\u6210\u7684\u3002<\/p>\n\n\n\n<p>\u8c03\u6574\u9608\u503c\u4f1a\u6539\u53d8\u8be5\u68c0\u6d4b\u7684 Ct \u503c\uff0c\u4f46\u5982\u679c\u9608\u503c\u8bbe\u7f6e\u5728\u63a8\u8350\u8303\u56f4\u5185\uff0c\u5219 Ct \u503c\u53d8\u5316\u662f\u4e00\u81f4\u7684\u3002\u5728\u4f7f\u7528 \u0394\u0394Ct \u65b9\u6cd5\u65f6\uff0c\u4e00\u81f4\u7684 Ct \u503c\u53d8\u5316\u4e0d\u4f1a\u663e\u8457\u5f71\u54cd\u6700\u7ec8\u7ed3\u679c\u6570\u91cf\u3002<\/p>\n\n\n\n<p>\u4e0d\u540c\u68c0\u6d4b\u4e4b\u95f4\u4e0d\u8981\u6c42\u4f7f\u7528\u76f8\u540c\u7684\u9608\u503c\uff0c\u4f46\u5982\u679c\u6bcf\u4e2a\u68c0\u6d4b\u90fd\u9002\u7528\uff0c\u8be5\u9608\u503c\u4e5f\u53ef\u4ee5\u4e3a\u4e86\u65b9\u4fbf\u8bbe\u4e3a\u76f8\u540c\u3002<\/p>\n\n\n\n<p>\u5efa\u8bae\u901a\u8fc7\u6269\u589e\u66f2\u7ebf\u56fe\u5bf9\u9608\u503c\u8fdb\u884c\u76ee\u89c6\u8bc4\u4f30\uff0c\u4ee5\u786e\u4fdd\u5176\u5904\u4e8e\u53ef\u63a5\u53d7\u8303\u56f4\uff0c\u5373\u4f7f\u91c7\u7528\u81ea\u52a8\u9608\u503c\u7b97\u6cd5\u4e5f\u662f\u5982\u6b64\u3002<strong>\u56fe4<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"369\" height=\"194\" src=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure204-8.jpg\" alt=\"Amplification plot showing thresholds\" class=\"wp-image-328\" title=\"Amplification plot showing thresholds\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure204-8.jpg 369w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/figure204-8-300x158.jpg 300w\" sizes=\"auto, (max-width: 369px) 100vw, 369px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u76f8\u5bf9\u9608\u503c\u6cd5\">\u76f8\u5bf9\u9608\u503c\u6cd5<\/h2>\n\n\n\n<p>\u4e00\u4e9b\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u8f6f\u4ef6\u7a0b\u5e8f\u63d0\u4f9b\u4e86\u4e00\u79cd\u8ba1\u7b97Ct\u503c\u7684\u66ff\u4ee3\u65b9\u6cd5\uff0c\u79f0\u4e3a\u76f8\u5bf9\u9608\u503c\u6cd5\u3002\u8be5\u65b9\u6cd5\u662f\u81ea\u52a8\u5316\u7684\uff0c\u56e0\u6b64\u65e0\u9700\u8bc4\u4f30\u6216\u4fee\u6539\u57fa\u7ebf\u6216\u9608\u503c\u8bbe\u7f6e\u3002\u8be5\u7b97\u6cd5\u4f1a\u8bc6\u522b\u53ef\u80fd\u7684\u6269\u589e\u4fe1\u53f7\uff0c\u5e76\u6839\u636e\u6bcf\u4e2a\u6269\u589e\u66f2\u7ebf\u7684\u5f62\u72b6\u5728\u4e00\u81f4\u7684\u4f4d\u7f6e\u8ba1\u7b97Ct\u503c\u3002\u4f7f\u7528\u76f8\u5bf9\u9608\u503c\u6cd5\u65f6\uff0cCt\u503c\u5c06\u5728\u8f6f\u4ef6\u4e2d\u663e\u793a\u4e3a\u201cCrt\u201d\u503c\uff0c\u4ee5\u6807\u8bc6\u6240\u91c7\u7528\u7684\u65b9\u6cd5\u3002<\/p>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u8f6f\u4ef6\u4f1a\u8ba1\u7b97\u8d28\u91cf\u63a7\u5236\u6570\u503c\uff0c\u8fd9\u4e9b\u6570\u503c\u53ef\u80fd\u5305\u62ecCq\u7f6e\u4fe1\u5ea6\u3001\u6269\u589e\u8bc4\u5206\u548c\u6269\u589e\u72b6\u6001\u3002\u5efa\u8bae\u8bc4\u4f30\u8fd9\u4e9b\u8d28\u91cf\u63a7\u5236\u6570\u503c\uff0c\u4ee5\u786e\u4fddCt\u8fd9\u4e9b\u6570\u503c\u6765\u81ea\u771f\u5b9e\u7684\u6269\u589e\u53cd\u5e94\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7b49\u6548ct\u503c\">\u7b49\u6548Ct\u503c<\/h2>\n\n\n\n<p>\u9664\u4e86Ct\u503c\u4e4b\u5916\uff0c\u4e00\u4e9b\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u8f6f\u4ef6\u7a0b\u5e8f\u8fd8\u4f1a\u663e\u793a\u201c\u7b49\u6548\u201dCt\u503c\uff0c\u5373\u5982\u679c\u68c0\u6d4b\u5177\u6709100%\u6307\u6570\u671f\u6548\u7387\u65f6\u5e94\u6709\u7684Ct\u503c\u3002\u5982\u679c\u68c0\u6d4b\u6548\u7387\u5728\u8f6f\u4ef6\u4e2d\u4fdd\u6301\u9ed8\u8ba4\u7684100%\uff0c\u5219Ct\u503c\u4e0e\u7b49\u6548Ct\u503c\u5c06\u5b8c\u5168\u4e00\u81f4\u3002<br><br> \u53ea\u6709\u5728\u6781\u5176\u8c28\u614e\u7684\u60c5\u51b5\u4e0b\u624d\u5e94\u5c06\u68c0\u6d4b\u6548\u7387\u4ece\u9ed8\u8ba4\u7684100%\u66f4\u6539\u3002\u5e38\u89c1\u7684\u4e00\u79cd\u8ba1\u7b97\u6307\u6570\u671f\u6548\u7387\u7684\u65b9\u6cd5\u662f\u57fa\u4e8e\u6807\u51c6\u66f2\u7ebf\u659c\u7387\uff0c\u4f46\u6807\u51c6\u66f2\u7ebf\u659c\u7387\u4f1a\u968f\u673a\u6ce2\u52a8\u3002\u5efa\u8bae\u4ec5\u4f7f\u7528\u5177\u6709100%\u6307\u6570\u671f\u6548\u7387\u7684\u68c0\u6d4b\u8fdb\u884c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\uff0c\u56e0\u4e3a\u4f4e\u6548\u7387\u68c0\u6d4b\u4f1a\u5e26\u6765\u4e0d\u5fc5\u8981\u7684\u590d\u6742\u6027\u3002\u4f8b\u5982\uff0c\u5982\u679c\u67d0\u4e00\u68c0\u6d4b\u6548\u7387\u8f83\u4f4e\uff0c\u5219\u51c6\u786e\u6d4b\u91cf\u5176\u6548\u7387\u5c06\u53d8\u5f97\u56f0\u96be\u3002\u6b64\u5916\uff0c\u4e3a\u4e86\u4fdd\u6301\u7075\u654f\u5ea6\uff0c\u5b9e\u65f6PCR\u5e38\u89c4\u4f7f\u7528\u768440\u4e2a\u5faa\u73af\u6b21\u6570\u4e5f\u9700\u8981\u589e\u52a0\u3002<br><br> \u672c\u6587\u4ec5\u6d45\u6790\u4e86\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\uff08qPCR\uff09\u7684\u57fa\u7840\u5185\u5bb9\u3002\u5982\u9700\u4e86\u89e3\u66f4\u591a\u4fe1\u606f\uff0c\u8bf7\u8bbf\u95ee\u4ee5\u4e0b\u6709\u7528\u8d44\u6e90\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/Documents\/PDFs\/PG1503-PJ9169-CO019879-Re-brand-Real-Time-PCR-Understanding-Ct-Value-Americas-FHR.pdf\" target=\"_blank\" rel=\"noopener\">\u7406\u89e3C<sub>t<\/sub>\u5e94\u7528\u8bf4\u660e<\/a><\/li>\n\n\n\n<li>Taq Talk \u89c6\u9891\uff0c\u7b2c3\u96c6\uff1a<a href=\"http:\/\/players.brightcove.net\/3663210762001\/XCI1AMd08_default\/index.html?videoId=6277032743001\" target=\"_blank\" rel=\"noopener\">\u7406\u89e3C<sub>t<\/sub>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4e2d\u7684\u6570\u503c<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/players.brightcove.net\/3663210762001\/XCI1AMd08_default\/index.html?videoId=6291526717001\" target=\"_blank\" rel=\"noopener\">Taq Talk \u89c6\u9891\uff0c\u7b2c12\u96c6\uff1a\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4e2d\u7684\u57fa\u7ebf<\/a><\/li>\n\n\n\n<li>Taq Talk \u89c6\u9891\uff0c\u7b2c13\u96c6\uff1a<a href=\"http:\/\/players.brightcove.net\/3663210762001\/XCI1AMd08_default\/index.html?videoId=6292686179001\" target=\"_blank\" rel=\"noopener\">\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u9608\u503c\u53ca\u5176\u8bbe\u7f6e\u4f4d\u7f6e<\/a><\/li>\n\n\n\n<li>Taq Talk \u89c6\u9891\uff0c\u7b2c14\u96c6\uff1a<a href=\"http:\/\/players.brightcove.net\/3663210762001\/XCI1AMd08_default\/index.html?videoId=6293923322001\" target=\"_blank\" rel=\"noopener\">\u5982\u4f55\u5224\u65adC<sub>t<\/sub>\u503c\u662f\u771f\u5b9e\u4fe1\u53f7\u8fd8\u662f\u80cc\u666f\u566a\u97f3<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/life-science\/digital-experience.html\" target=\"_blank\" rel=\"noopener\">qPCR\u624b\u518c2.0\uff0c\u6570\u5b57\u4f53\u9a8c<\/a><\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4f5c\u8005\uff1aJohn Pfeifer 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