{"id":281,"date":"2026-02-11T06:20:44","date_gmt":"2026-02-11T06:20:44","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=281"},"modified":"2026-03-10T06:22:09","modified_gmt":"2026-03-10T06:22:09","slug":"plasmid-prep-protocol-guide-to-key-steps-and-materials","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/plasmid-prep-protocol-guide-to-key-steps-and-materials\/","title":{"rendered":"\u8d28\u7c92\u63d0\u53d6\u64cd\u4f5c\u6d41\u7a0b\uff1a\u5173\u952e\u6b65\u9aa4\u4e0e\u6750\u6599\u6307\u5357"},"content":{"rendered":"\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/08\/Plasmid-Purification-Feature-Image-1.jpg\" alt=\"Plasmid prep protocol\" class=\"wp-image-4914\" \/><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u672c\u6307\u5357\u6982\u8ff0\u4e86\u8d28\u7c92\u63d0\u53d6\u7684\u64cd\u4f5c\u6d41\u7a0b\uff0c\u91cd\u70b9\u4ecb\u7ecd\u4e86\u5b9e\u73b0\u6700\u4f73\u8d28\u7c92\u4ea7\u91cf\u548c\u7eaf\u5ea6\u6240\u9700\u7684\u5173\u952e\u6750\u6599\u548c\u6b65\u9aa4\u3002<\/p>\n\n\n\n<p>\n\u4ece\u624b\u52a8\u65b9\u6cd5\u5230\u81ea\u52a8\u5316\u89e3\u51b3\u65b9\u6848\uff0c\u4e86\u89e3\u5982\u4f55\u4f18\u5316\u8d28\u7c92\u7eaf\u5316\u6d41\u7a0b\uff0c\u4ee5\u5728\u751f\u7269\u6280\u672f\u7814\u7a76\u5e94\u7528\u4e2d\u83b7\u5f97\u9ad8\u8d28\u91cf\u7684\u540e\u7eed\u7ed3\u679c\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-yoast-seo-table-of-contents yoast-table-of-contents\"><h2>\u76ee\u5f55<\/h2><ul><li><a href=\"#h-\u4ec0\u4e48\u662f\u8d28\u7c92-\u5b83\u4eec\u5728\u751f\u7269\u6280\u672f\u7814\u7a76\u4e2d\u7684\u7528\u9014\u662f\u4ec0\u4e48\" data-level=\"2\">\u4ec0\u4e48\u662f\u8d28\u7c92\uff0c\u5b83\u4eec\u5728\u751f\u7269\u6280\u672f\u7814\u7a76\u4e2d\u7684\u7528\u9014\u662f\u4ec0\u4e48\uff1f<\/a><\/li><li><a href=\"#h-\u7406\u89e3\u8d28\u7c92\u514b\u9686\u8fc7\u7a0b\" data-level=\"2\">\u7406\u89e3\u8d28\u7c92\u514b\u9686\u8fc7\u7a0b<\/a><\/li><li><a href=\"#h-\u5f71\u54cd\u8d28\u7c92\u63d0\u53d6\u4ea7\u91cf\u548c\u7eaf\u5ea6\u7684\u56e0\u7d20\" data-level=\"2\">\u5f71\u54cd\u8d28\u7c92\u63d0\u53d6\u4ea7\u91cf\u548c\u7eaf\u5ea6\u7684\u56e0\u7d20<\/a><\/li><li><a href=\"#h-\u8d28\u7c92\u63d0\u53d6\u6240\u7528\u6750\u6599\" data-level=\"2\">\u8d28\u7c92\u63d0\u53d6\u6240\u7528\u6750\u6599<\/a><ul><li><a href=\"#h-\u624b\u52a8\u5904\u7406\u5de5\u5177\" data-level=\"3\">\u624b\u52a8\u5904\u7406\u5de5\u5177<\/a><\/li><li><a href=\"#h-\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\" data-level=\"3\">\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li><li><a href=\"#h-\u8d28\u7c92\u63d0\u53d6\u81ea\u52a8\u5316\" data-level=\"3\">\u8d28\u7c92\u63d0\u53d6\u81ea\u52a8\u5316<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u8d28\u7c92\u63d0\u53d6\u6d41\u7a0b\u4e2d\u7684\u5173\u952e\u6b65\u9aa4\" data-level=\"2\">\u8d28\u7c92\u63d0\u53d6\u6d41\u7a0b\u4e2d\u7684\u5173\u952e\u6b65\u9aa4<\/a><ul><li><a href=\"#h-1-\u51c6\u5907\u8fc7\u591c\u7684\u7ec6\u83cc\u57f9\u517b\u7269\" data-level=\"3\">1. \u51c6\u5907\u8fc7\u591c\u7684\u7ec6\u83cc\u57f9\u517b\u7269\u3002<\/a><\/li><li><a href=\"#h-2-\u6536\u83b7\u7ec6\u83cc\u7ec6\u80de\" data-level=\"3\">2. \u6536\u83b7\u7ec6\u83cc\u7ec6\u80de<\/a><\/li><li><a href=\"#h-3-\u8bf1\u5bfc\u7ec6\u80de\u88c2\u89e3\" data-level=\"3\">3. \u8bf1\u5bfc\u7ec6\u80de\u88c2\u89e3<\/a><\/li><li><a href=\"#h-4-\u6f84\u6e05\u88c2\u89e3\u6db2\" data-level=\"3\">4. \u6f84\u6e05\u88c2\u89e3\u6db2<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u66f4\u591a\u8d28\u7c92\u63d0\u53d6\u8d44\u6e90\" data-level=\"2\">\u66f4\u591a\u8d28\u7c92\u63d0\u53d6\u8d44\u6e90<\/a><\/li><\/ul><\/div>\n\n\n\n<div 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style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6acaa39c8cc64&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6acaa39c8cc64\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/08\/Fig1-Plasmid-Prep-1024x435.png\" alt=\"Overview of the general molecular biology workflow ending in plasmid prep\" class=\"wp-image-4915\" 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class=\"wp-element-caption\"><strong>\u5206\u5b50\u751f\u7269\u5b66\u5e38\u89c4\u6d41\u7a0b\u6982\u8ff0\u3002<\/strong>\u76ee\u6807DNA\uff08\u79f0\u4e3a\u63d2\u5165\u7247\u6bb5\uff09\u901a\u5e38\u901a\u8fc7PCR\u5236\u5907\uff0c\u7136\u540e\u901a\u8fc7\u8fde\u63a5\u53cd\u5e94\u63d2\u5165\u5230\u8f7d\u4f53\u9aa8\u67b6\u4e2d\u3002\u4e00\u65e6\u5c06\u63d2\u5165\u7247\u6bb5\u52a0\u5165\u5230\u8d28\u7c92\u4e2d\uff0c\u5c31\u4f1a\u5c06\u5176\u8f6c\u5316\u5230\u611f\u53d7\u6001\u7ec6\u80de\u4e2d\uff0c\u968f\u540e\u5bf9\u83cc\u843d\u8fdb\u884c\u7b5b\u9009\u5e76\u7528\u4e8e\u8d28\u7c92\u7eaf\u5316\u3002\u7eaf\u5316\u540e\u7684\u8d28\u7c92\u4f1a\u88ab\u5206\u6790\uff0c\u7136\u540e\u7528\u4e8e\u6240\u9700\u7684\u4e0b\u6e38\u5e94\u7528\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5f71\u54cd\u8d28\u7c92\u63d0\u53d6\u4ea7\u91cf\u548c\u7eaf\u5ea6\u7684\u56e0\u7d20\">\u5f71\u54cd\u8d28\u7c92\u63d0\u53d6\u4ea7\u91cf\u548c\u7eaf\u5ea6\u7684\u56e0\u7d20<\/h2>\n\n\n\n<p>\u6709\u51e0\u4e2a\u56e0\u7d20\u4f1a\u5f71\u54cd\u8d28\u7c92\u4ea7\u91cf\u548c\u7eaf\u5316\u540e\u8d28\u7c92DNA\u7684\u7eaf\u5ea6\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u8d28\u7c92\u5927\u5c0f\u4e0e\u8bbe\u8ba1<\/strong>\uff1a\u8f7d\u4f53\u9aa8\u67b6\u7684\u9009\u62e9\u3001\u63d2\u5165\u7247\u6bb5\u7684\u5927\u5c0f\u4ee5\u53caDNA\u63d2\u5165\u7247\u6bb5\u5bf9\u4e8e\u8d28\u7c92\u4ea7\u91cf\u975e\u5e38\u91cd\u8981\u3002<\/li>\n\n\n\n<li><strong>\u7ec6\u83cc\u83cc\u682a\u7684\u9009\u62e9<\/strong>\u4e0d\u540c\u83cc\u682a\u5177\u6709\u4e0d\u540c\u7684\u4f18\u52bf\uff0c\u4f8b\u5982\u589e\u5f3a\u8d28\u7c92\u7a33\u5b9a\u6027\u6216\u63d0\u9ad8\u8f6c\u5316\u6548\u7387\u3002<\/li>\n\n\n\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\u6700\u4f73\u751f\u957f\u6761\u4ef6\uff0c\u5305\u62ec\u6e29\u5ea6\u3001pH\u503c\u3001\u6c27\u6c14\u6c34\u5e73\u3001\u751f\u957f\u65f6\u95f4\u548c\u6447\u5e8a\u901f\u5ea6\uff0c\u5bf9\u4e8e\u83b7\u5f97\u9ad8\u4ea7\u91cf\u7684\u8d28\u7c92\u81f3\u5173\u91cd\u8981\u3002<\/li>\n\n\n\n<li><strong>\u6297\u751f\u7d20\u4f7f\u7528<\/strong>\u6b63\u786e\u7684\u6297\u751f\u7d20\u6d53\u5ea6\u6709\u52a9\u4e8e\u7ef4\u6301\u9009\u62e9\u538b\u529b\uff0c\u8fd9\u5bf9\u4e8e\u8d28\u7c92\u4fdd\u7559\u81f3\u5173\u91cd\u8981\u3002<\/li>\n\n\n\n<li><strong>\u590d\u5236\u8d77\u70b9<\/strong>\u590d\u5236\u8d77\u70b9\uff08ori\uff09\u51b3\u5b9a\u4e86\u8d28\u7c92\u7684\u62f7\u8d1d\u6570\uff0c\u4ece\u800c\u5f71\u54cd\u4ea7\u91cf\u3002\u9ad8\u62f7\u8d1d\u8d28\u7c92\u5982 pUC \u80fd\u4ea7\u751f\u5927\u91cf\u62f7\u8d1d\uff0c\u800c\u4f4e\u62f7\u8d1d\u8d28\u7c92\u5982 pBR322 \u62f7\u8d1d\u6570\u91cf\u8f83\u5c11\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u63d0\u53d6\u6240\u7528\u6750\u6599\">\u8d28\u7c92\u63d0\u53d6\u6240\u7528\u6750\u6599<\/h2>\n\n\n\n<p>\u5982\u4eca\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u9009\u62e9\u4f20\u7edf\u7684\u624b\u52a8\u8d28\u7c92\u7eaf\u5316\u6d41\u7a0b\u3001\u73b0\u6210\u8bd5\u5242\u76d2\u6216\u5168\u81ea\u52a8\u65e0\u63a5\u89e6\u7cfb\u7edf\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u624b\u52a8\u5904\u7406\u5de5\u5177\">\u624b\u52a8\u5904\u7406\u5de5\u5177<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u57f9\u517b\u57fa\"><strong>\u57f9\u517b\u57fa<\/strong><\/h4>\n\n\n\n<p>\u53ef\u4ee5\u4f7f\u7528\u51e0\u79cd\u4e0d\u540c\u7c7b\u578b\u7684\u57f9\u517b\u57fa\u6765\u57f9\u517b\u7528\u4e8e\u8d28\u7c92\u7eaf\u5316\u7684\u8fc7\u591c\u7ec6\u83cc\u57f9\u517b\u7269\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Gibco\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/10855021\" target=\"_blank\" rel=\"noreferrer noopener\">\u6eb6\u539f\u6027\u8089\u6c64\uff08LB\uff09<\/a>\u662f\u6700\u5e38\u7528\u7684\u7ec6\u83cc\u57f9\u517b\u57fa\u3002LB \u57f9\u517b\u57fa\u7531\u86cb\u767d\u80e8\u3001\u9175\u6bcd\u63d0\u53d6\u7269\u548c\u6c2f\u5316\u94a0\u914d\u5236\u800c\u6210\u3002\u6839\u636e\u6c2f\u5316\u94a0\u7684\u6d53\u5ea6\uff0cLB \u57f9\u517b\u57fa\u8fdb\u4e00\u6b65\u5206\u4e3a LB-Miller\u3001LB-Lennox \u548c\u4f4e\u76d0 LB\u3002<\/li>\n\n\n\n<li>Gibco\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A1374301?SID=srch-srp-A1374301\" target=\"_blank\" rel=\"noreferrer noopener\">\u7279\u7ea7\u8089\u6c64\uff08TB\uff09<\/a>\u662f\u4e00\u79cd\u8425\u517b\u4e30\u5bcc\u7684\u57f9\u517b\u57fa\uff0c\u542b\u6709\u66f4\u9ad8\u6d53\u5ea6\u7684\u86cb\u767d\u80e8\u548c\u9175\u6bcd\u63d0\u53d6\u7269\uff0c\u63d0\u4f9b\u4e30\u5bcc\u7684\u6c2e\u6e90\u3001\u7ef4\u751f\u7d20\u548c\u77ff\u7269\u8d28\u3002TB \u57f9\u517b\u57fa\u80fd\u4fc3\u8fdb\u7ec6\u83cc\u751f\u957f\uff0c\u5e76\u652f\u6301\u66f4\u9ad8\u5bc6\u5ea6\u7684\u7ec6\u83cc\u57f9\u517b\uff0c\u4ece\u800c\u5728\u8f83\u77ed\u65f6\u95f4\u5185\u83b7\u5f97\u66f4\u591a\u7ec6\u80de\u3002<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/J63227.K2\" target=\"_blank\" rel=\"noreferrer noopener\">2X YT \u57f9\u517b\u57fa\u2122<\/a> \u4e0e\u5178\u578b LB \u57f9\u517b\u57fa\u76f8\u6bd4\uff0c\u5176\u9175\u6bcd\u63d0\u53d6\u7269\u6d53\u5ea6\u52a0\u500d\uff082X\uff09\uff0c\u53ef\u7528\u4e8e\u4fc3\u8fdb\u66f4\u9ad8\u5bc6\u5ea6\u7ec6\u80de\u7684\u751f\u957f\u548c\u7ef4\u6301\u8f83\u957f\u65f6\u95f4\u7684\u751f\u957f\u671f\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6acaa39c8da54&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6acaa39c8da54\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/08\/Fig3-PlasmidPrep.png\" alt=\"LB compared to TB growth media in plasmid prep protocols\" class=\"wp-image-4917\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><strong>LB\u4e0eTB\u57f9\u517b\u57fa\u7684\u6bd4\u8f83\u3002<\/strong>\u5229\u7528LB\u548cTB\u57f9\u517b\u57fa\uff0c\u4f7f\u7528MagMAX Pro HT NoSpin Miniprep\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u7eaf\u5316\u4e86\u5e02\u552e\u8d28\u7c92DNA\u3002TB\u57f9\u517b\u57fa\u7684OD600\u8bfb\u6570\u4e3a5.1\uff0c\u800cLB\u4e3a3.1\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u7f13\u51b2\u6db2\u548c\u6eb6\u6db2\"><strong>\u7f13\u51b2\u6db2\u548c\u6eb6\u6db2<\/strong><\/h4>\n\n\n\n<p>\u9700\u8981\u591a\u79cd\u7f13\u51b2\u6db2\u548c\u8bd5\u5242\u6765\u91cd\u60ac\u7ec6\u83cc\u6c89\u6dc0\u3001\u88c2\u89e3\u7ec6\u83cc\u7ec6\u80de\u3001\u4e2d\u548c\u6eb6\u6db2\u4ee5\u6c89\u6dc0DNA\u3001\u6d17\u6da4\u6742\u8d28\u4ee5\u53ca\u6d17\u8131\u7eaf\u5316\u540e\u7684\u8d28\u7c92DNA\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5176\u4ed6\u5b9e\u9a8c\u5ba4\u8bbe\u5907\"><strong>\u5176\u4ed6\u5b9e\u9a8c\u5ba4\u8bbe\u5907<\/strong><\/h4>\n\n\n\n<p>\u901a\u5e38\u9700\u8981\u79bb\u5fc3\u673a\u3001\u771f\u7a7a\u8fc7\u6ee4\u88c5\u7f6e\u6216\u91cd\u529b\u6d41\u8bbe\u5907\u7b49\u8fdb\u884c\u624b\u52a8\u64cd\u4f5c\uff0c\u4ee5\u7eaf\u5316\u8d28\u7c92DNA\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92dna\u7ed3\u5408\u8868\u9762\"><strong>\u8d28\u7c92DNA\u7ed3\u5408\u8868\u9762<\/strong><\/h4>\n\n\n\n<p>\u9700\u8981\u7845\u80f6\u6216\u9634\u79bb\u5b50\u4ea4\u6362\u819c\u3001\u6811\u8102\u3001\u78c1\u73e0\u6216\u5176\u4ed6\u8d28\u7c92DNA\u7ed3\u5408\u57fa\u6750\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5206\u5149\u5149\u5ea6\u8ba1\"><strong>\u5206\u5149\u5149\u5ea6\u8ba1<\/strong><\/h4>\n\n\n\n<p>\u7528\u4e8e\u6d4b\u91cfDNA\u6d53\u5ea6\u548c\u7eaf\u5ea6\u7684\u4eea\u5668\u5305\u62ecThermo Scientific NanoDrop\u3001\u9176\u6807\u4eea\u6216\u5176\u4ed6\u5206\u5149\u5149\u5ea6\u8ba1\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u4e0e\u6210\u50cf\"><strong>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u4e0e\u6210\u50cf<\/strong><\/h4>\n\n\n\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5de5\u5177\u7528\u4e8e\u68c0\u6d4b\u8d28\u7c92DNA\u7684\u5b8c\u6574\u6027\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u79bb\u5fc3\u7ba1\"><strong>\u79bb\u5fc3\u7ba1<\/strong><\/h4>\n\n\n\n<p>\u5728\u8d28\u7c92\u7eaf\u5316\u8fc7\u7a0b\u4e2d\uff0c\u4f1a\u5728\u591a\u4e2a\u6b65\u9aa4\u4e2d\u4f7f\u7528\u591a\u79cd\u53ef\u5bb9\u7eb3\u4e0d\u540c\u4f53\u79ef\u8bd5\u5242\u548c\u7f13\u51b2\u6db2\u7684\u79bb\u5fc3\u7ba1\uff0c\u5305\u62ec\u5fae\u91cf\u79bb\u5fc3\u7ba1\u3001\u819c\u67f1\u7ba1\u3001\u6f84\u6e05\u8fc7\u6ee4\u7ba1\u548c\u6536\u96c6\u7ba1\u3002\u6240\u6709\u6240\u9700\u7684\u79bb\u5fc3\u7ba1\u90fd\u5305\u542b\u5728\u8d28\u7c92\u7eaf\u5316\u8bd5\u5242\u76d2\u4e2d\u3002\u8fd9\u4e9b\u79bb\u5fc3\u7ba1\u901a\u5e38\u7531\u8010\u7528\u4e14\u6297\u6e17\u6f0f\u7684\u6750\u6599\u5236\u6210\uff0c\u5982\u805a\u4e19\u70ef\u6216\u805a\u4e59\u70ef\u3002\u8fd9\u4e9b\u79bb\u5fc3\u7ba1\u4e5f\u6709\u65e0\u5185\u6bd2\u7d20\u7248\u672c\uff0c\u4ee5\u6ee1\u8db3\u66f4\u9ad8\u7eaf\u5ea6\u7684\u9700\u6c42\u3002<\/p>\n\n\n\n<p>\u901a\u8fc7\u9075\u5faa\u8fd9\u4e9b\u6b65\u9aa4\u5e76\u4f7f\u7528\u5408\u9002\u7684\u6750\u6599\uff0c\u60a8\u53ef\u4ee5\u6709\u6548\u5730\u7eaf\u5316\u8d28\u7c92DNA\uff0c\u7528\u4e8e\u5404\u79cd\u540e\u7eed\u5e94\u7528\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\">\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/h3>\n\n\n\n<p>\u4f20\u7edf\u7684\u67f1\u5f0f\u65b9\u6cd5\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4ece\u5c0f\u89c4\u6a21\u8d28\u7c92\u5206\u79bb\u5230\u5546\u4e1a\u89c4\u6a21\u6269\u5927\u7684\u7075\u6d3b\u9009\u62e9\u3002\u624b\u52a8\u7eaf\u5316\u8bd5\u5242\u76d2\u7684\u9009\u62e9\u53d6\u51b3\u4e8e\u5b9e\u9a8c\u5ba4\u5bf9\u8d77\u59cb\u57f9\u517b\u4f53\u79ef\u4ee5\u53ca\u540e\u7eed\u5e94\u7528\u6240\u9700\u8d28\u7c92\u4ea7\u91cf\u7684\u9700\u6c42\u3002<\/p>\n\n\n\n<p>\u4ee5\u4e0b\u662f\u9488\u5bf9\u4e0d\u540c\u8d77\u59cb\u57f9\u517b\u4f53\u79ef\u548c\u9884\u671f\u8d28\u7c92\u4ea7\u91cf\u7684\u5404\u7c7b\u67f1\u5f0f\u89e3\u51b3\u65b9\u6848\u6458\u8981\uff1a<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5c0f\u91cf\u63d0\u53d6-1-5ml-\u6700\u9ad840\u00b5g\"><strong>\u5c0f\u91cf\u63d0\u53d6\uff081-5mL\uff1b\u6700\u9ad840\u00b5g\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210011?SID=srch-srp-K210011\" target=\"_blank\" rel=\"noreferrer noopener\">Invitrogen\u2122 PureLink\u2122 \u5feb\u901f\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210002?SID=srch-srp-K210002\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure \u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K0502\" target=\"_blank\" rel=\"noreferrer noopener\">Thermo Scientific\u2122 GeneJET \u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u4e2d\u91cf\u63d0\u53d6-25-100ml-\u9ad8\u8fbe400\u00b5g\"><strong>\u4e2d\u91cf\u63d0\u53d6\uff0825-100mL\uff1b\u9ad8\u8fbe400\u00b5g\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210004?SID=srch-srp-K210004\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure \u8d28\u7c92\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210014?SID=srch-srp-K210014\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure \u8d28\u7c92\u8fc7\u6ee4\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K0481?SID=srch-srp-K0481\" target=\"_blank\" rel=\"noreferrer noopener\">GeneJET \u8d28\u7c92\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5927\u91cf\u63d0\u53d6-100-500ml-\u9ad8\u8fbe1-5mg\"><strong>\u5927\u91cf\u63d0\u53d6\uff08100-500mL\uff1b\u9ad8\u8fbe1.5mg\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210007?SID=srch-srp-K210007\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure \u8d28\u7c92\u5927\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A33073?SID=srch-srp-A33073\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink Expi \u65e0\u5185\u6bd2\u7d20 Maxi \u7ea7\u8d28\u7c92\u7eaf\u5316\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K0491?SID=srch-srp-K0491\" target=\"_blank\" rel=\"noreferrer noopener\">GeneJET \u8d28\u7c92\u5927\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5927\u5bb9\u91cf\u63d0\u53d6-500\u6beb\u5347-2-5\u5347-\u6700\u9ad8\u53ef\u8fbe5\u6beb\u514b\"><strong>\u5927\u5bb9\u91cf\u63d0\u53d6\uff08500\u6beb\u5347-2.5\u5347\uff1b\u6700\u9ad8\u53ef\u8fbe5\u6beb\u514b\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210008XP?SID=srch-srp-K210008XP\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure Expi \u5927\u5bb9\u91cf\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A31232?SID=srch-srp-A31232\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink Expi \u65e0\u5185\u6bd2\u7d20\u5927\u5bb9\u91cf\u8d28\u7c92\u7eaf\u5316\u8bd5\u5242\u76d2<\/a><\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u8d85\u5927\u5bb9\u91cf\u63d0\u53d6-2-5-5\u5347-\u6700\u9ad8\u53ef\u8fbe15\u6beb\u514b\"><strong>\u8d85\u5927\u5bb9\u91cf\u63d0\u53d6\uff082.5-5\u5347\uff1b\u6700\u9ad8\u53ef\u8fbe15\u6beb\u514b\uff09<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A31233?SID=srch-srp-A31233\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink Expi \u65e0\u5185\u6bd2\u7d20\u8d85\u5927\u5bb9\u91cf\u8d28\u7c92\u7eaf\u5316\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K210009XP?SID=srch-srp-K210009XP\" target=\"_blank\" rel=\"noreferrer noopener\">PureLink HiPure Expi \u8d85\u5927\u5bb9\u91cf\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u63d0\u53d6\u81ea\u52a8\u5316\">\u8d28\u7c92\u63d0\u53d6\u81ea\u52a8\u5316<\/h3>\n\n\n\n<p>\u6700\u65b0\u7684\u81ea\u52a8\u5316\u8d28\u7c92\u5206\u79bb\u6280\u672f\u76f8\u6bd4\u624b\u52a8\u65b9\u6cd5\u53ef\u4ee5\u5e2e\u52a9\u63d0\u9ad8\u6548\u7387\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58309\">Applied Biosystems\u2122 MagMAX\u2122 ProHT NoSpin Miniprep \u8d28\u7c92\u5206\u79bb\u8bd5\u5242\u76d2<\/a>\u4f8b\u5982\uff0c\u5728\u6536\u83b7\u7ec6\u83cc\u7ec6\u80de\u65f6\uff0c\u5b8c\u5168\u7701\u53bb\u4e86\u79bb\u5fc3\u6b65\u9aa4\u3002\u8be5\u8bd5\u5242\u76d2\u91c7\u7528\u53cc\u53c2\u6570\u78c1\u73e0\u6cd5\uff0c\u5176\u4e2d\u4e00\u7ec4\u78c1\u73e0\u4e0e\u7ec6\u83cc\u7ed3\u5408\uff0c\u53e6\u4e00\u7ec4\u78c1\u73e0\u4e0e\u8d28\u7c92DNA\u7ed3\u5408\uff0c\u4ece\u800c\u6d17\u8131\u51fa\u66f4\u9ad8\u8d28\u91cf\u7684\u8d28\u7c92DNA\u3002<\/p>\n\n\n\n<p>Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/automated-plasmid-purification.html?ef_id=CjwKCAjwsZPDBhBWEiwADuO6y2YOtwA_XBjltxWYlQrh-0jSAOpe6TAYUhgDQtL2YVPlEzP5dTEwFxoCjJIQAvD_BwE:G:s&amp;s_kwcid=AL!3652!3!706022375173!e!!g!!plasmid%20pro!21456979146!164346234709&amp;cid=bid_sap_rst_r01_co_cp1362_pjt0000_bid00000_0se_gaw_bt_lgn_ins&amp;gad_source=1&amp;gad_campaignid=21456979146&amp;gbraid=0AAAAADxi_GSx0x7INMN8HSC4MgK27Pjhn&amp;gclid=CjwKCAjwsZPDBhBWEiwADuO6y2YOtwA_XBjltxWYlQrh-0jSAOpe6TAYUhgDQtL2YVPlEzP5dTEwFxoCjJIQAvD_BwE\" target=\"_blank\" rel=\"noreferrer noopener\">KingFisher\u2122 PlasmidPro Maxi Processer<\/a>\u662f\u53e6\u4e00\u79cd\u5de5\u5177\uff0c\u53ef\u4ee5\u8ba9\u7814\u7a76\u4eba\u5458\u5728\u7cfb\u7edf\u81ea\u52a8\u5316\u51e0\u4e4e\u6574\u4e2a\u7eaf\u5316\u6d41\u7a0b\u65f6\u65e0\u9700\u770b\u7ba1\u2014\u2014\u4ece\u57f9\u517b\u5230\u8f6c\u67d3\u7ea7\u7eaf\u5316\u8d28\u7c92DNA\u53ea\u9700\u4e0d\u523075\u5206\u949f\uff0c\u4e14\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u4ec5\u4e3a5\u5206\u949f\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u8d28\u7c92\u63d0\u53d6\u6d41\u7a0b\u4e2d\u7684\u5173\u952e\u6b65\u9aa4\">\u8d28\u7c92\u63d0\u53d6\u6d41\u7a0b\u4e2d\u7684\u5173\u952e\u6b65\u9aa4<\/h2>\n\n\n\n<p>\u8d28\u7c92\u7eaf\u5316\u53ef\u80fd\u662f\u4e00\u9879\u52b3\u52a8\u5bc6\u96c6\u4e14\u8017\u65f6\u7684\u624b\u52a8\u8fc7\u7a0b\u3002<\/p>\n\n\n\n<p>\u624b\u52a8\u8d28\u7c92\u5206\u79bb\u548c\u7eaf\u5316\u6280\u672f\u901a\u5e38\u5305\u62ec\u4ee5\u4e0b\u6b65\u9aa4\uff1a<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-\u51c6\u5907\u8fc7\u591c\u7684\u7ec6\u83cc\u57f9\u517b\u7269\">1. \u51c6\u5907\u8fc7\u591c\u7684\u7ec6\u83cc\u57f9\u517b\u7269\u3002<\/h3>\n\n\n\n<p>\u6b64\u6b65\u9aa4\u7528\u4e8e\u57f9\u517b\u7ec6\u83cc\u7fa4\u4f53\uff0c\u4ee5\u6536\u83b7\u5206\u79bb\u7684\u8d28\u7c92DNA\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u63a5\u79cd\uff1a<\/strong>\u5c06\u5355\u4e2a\u7ec6\u83cc\u83cc\u843d\u63a5\u79cd\u5230\u5c11\u91cf\u6db2\u4f53\u57f9\u517b\u57fa\u4e2d\uff0c\u5236\u5907\u8d77\u59cb\u57f9\u517b\u7269\uff0c\u5e76\u572837\u00b0C\u3001225 rpm\u6761\u4ef6\u4e0b\u57f9\u517b4-8\u5c0f\u65f6\u3002\u4f7f\u7528\u8be5\u8d77\u59cb\u57f9\u517b\u7269\u63a5\u79cd\u66f4\u5927\u91cf\u7684\u8fc7\u591c\u57f9\u517b\uff0c\u7528\u4e8e\u8d28\u7c92\u7eaf\u5316\u3002<\/li>\n\n\n\n<li><strong>\u751f\u957f\uff1a<\/strong>\u5728\u6700\u4f73\u6761\u4ef6\u4e0b\u5b75\u80b2\u57f9\u517b\u7269\uff08\u901a\u5e38\u572837\u00b0C\u6447\u5e8a\u4e0a\uff09\uff0c\u76f4\u5230\u8fbe\u5230\u6240\u9700\u7684\u7ec6\u80de\u5bc6\u5ea6\uff08\u901a\u5e38\u4ee5OD600\u6d4b\u91cf\uff09\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u6210\u529f\u5236\u5907\u8d77\u59cb\u57f9\u517b\u7269\u7684\u5c0f\u8d34\u58eb\uff1a<\/strong>\u4f7f\u7528\u5355\u4e2a\u83cc\u843d\u5236\u5907\u7684\u8d77\u59cb\u57f9\u517b\u7269\u76f8\u6bd4\u76f4\u63a5\u4ece\u51b7\u51bb\u83cc\u682a\u5f00\u59cb\u8fc7\u591c\u7ec6\u83cc\u57f9\u517b\uff0c\u80fd\u83b7\u5f97\u66f4\u7406\u60f3\u7684\u751f\u957f\u6761\u4ef6\u3002\u8d77\u59cb\u57f9\u517b\u4e2d\u7684\u7ec6\u80de\u5904\u4e8e\u6d3b\u8dc3\u751f\u957f\u671f\uff0c\u5373\u6307\u6570\u671f\uff0c\u975e\u5e38\u9002\u5408\u7528\u4e8e\u65b0\u57f9\u517b\u7684\u542f\u52a8\u3002<\/p>\n\n\n\n<p>\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u51b7\u51bb\u83cc\u682a\u4e2d\u7684\u7ec6\u80de\u7531\u4e8e\u51b7\u51bb\u548c\u89e3\u51bb\u8fc7\u7a0b\u5e26\u6765\u7684\u538b\u529b\uff0c\u53ef\u80fd\u5b58\u6d3b\u7387\u964d\u4f4e\u2014\u2014\u5bfc\u81f4\u751f\u957f\u901f\u5ea6\u53d8\u6162\u3001\u6ede\u540e\u671f\u5ef6\u957f\u3002\u9ad8\u62f7\u8d1d\u8d28\u7c92\u901a\u5e38\u9700\u898112-16\u5c0f\u65f6\u751f\u957f\uff0c\u800c\u4f4e\u62f7\u8d1d\u8d28\u7c92\u53ef\u80fd\u9700\u898120\u5c0f\u65f6\u6216\u66f4\u4e45\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6acaa39c8f77c&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6acaa39c8f77c\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/08\/Fig2-Plasmid-Prep-1-1024x693.png\" alt=\"Growth curves from starter culture compared to frozen stock in plasmid prep\" class=\"wp-image-4918\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><strong>\u56fe2. \u8d77\u59cb\u57f9\u517b\u4e0e\u51b7\u51bb\u83cc\u682a\u7684\u751f\u957f\u66f2\u7ebf\u5bf9\u6bd4\u3002<\/strong>\u8d77\u59cb\u57f9\u517b\uff08\u767d\u5929\u57f9\u517b\u8fdb\u5165\u8fc7\u591c\u57f9\u517b\uff0c\u5728\u7ea610.5-11\u5c0f\u65f6\u8fbe\u5230\u5cf0\u503c\uff0c\u7136\u540e\u7a33\u5b9a\u4e0b\u964d\u2014\u2014\u56e0\u6ca1\u6709\u51b7\u51bb\/\u89e3\u51bb\u5468\u671f\uff0c\u6240\u4ee5\u5176\u8fbe\u5230\u5cf0\u503c\u5149\u5bc6\u5ea6\uff08OD\uff09\u7684\u6ede\u540e\u65f6\u95f4\u8f83\u77ed\uff09\u3002\u7518\u6cb9\u51b7\u51bb\u83cc\u682a\u8fdb\u5165\u8fc7\u591c\u57f9\u517b\u5728\u7ea615\u5c0f\u65f6\u8fbe\u5230\u5cf0\u503c\uff0c\u4f46\u572812-14\u5c0f\u65f6\u8fdb\u5165\u7a33\u5b9a\u9759\u6b62\u671f\u2014\u2014\u5176\u6ede\u540e\u671f\u8f83\u957f\uff0c\u540c\u65f6\u4e5f\u6709\u76f8\u5e94\u8f83\u957f\u7684\u9759\u6b62\u671f\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-\u6536\u83b7\u7ec6\u83cc\u7ec6\u80de\">2. \u6536\u83b7\u7ec6\u83cc\u7ec6\u80de<\/h3>\n\n\n\n<p>\u6b64\u6b65\u9aa4\u5c06\u6eb6\u6db2\u4e2d\u7684\u7ec6\u83cc\u7ec6\u80de\u6536\u96c6\u8d77\u6765\uff0c\u5f62\u6210\u6c89\u6dc0\uff0c\u4ee5\u4fbf\u8fdb\u4e00\u6b65\u5206\u6790\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u79bb\u5fc3\uff1a<\/strong>\u901a\u8fc7\u79bb\u5fc3\u57f9\u517b\u7269\u6536\u96c6\u7ec6\u83cc\u7ec6\u80de\uff0c\u5e76\u5f03\u53bb\u4e0a\u6e05\u6db2\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-\u8bf1\u5bfc\u7ec6\u80de\u88c2\u89e3\">3. \u8bf1\u5bfc\u7ec6\u80de\u88c2\u89e3<\/h3>\n\n\n\n<p>\u6b64\u6b65\u9aa4\u7684\u76ee\u7684\u662f\u6eb6\u89e3\u7ec6\u83cc\u5e76\u91ca\u653e\u5176\u5185\u5bb9\u7269\uff0c\u5305\u62ec\u9057\u4f20\u8d28\u7c92\u6750\u6599\uff0c\u4ee5\u4fbf\u8fdb\u4e00\u6b65\u7eaf\u5316\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u91cd\u60ac\uff1a<\/strong>\u7528\u91cd\u60ac\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u542b\u6709Tris-HCl\u3001EDTA\u548cRNase A\uff09\u5c06\u7ec6\u80de\u6c89\u6dc0\u91cd\u60ac\u3002<\/li>\n\n\n\n<li><strong>\u88c2\u89e3\uff1a<\/strong>\u52a0\u5165\u88c2\u89e3\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u542b\u6709NaOH\u548cSDS\uff09\uff0c\u4ee5\u7834\u574f\u7ec6\u80de\u5e76\u91ca\u653e\u8d28\u7c92DNA\u3002<\/li>\n\n\n\n<li><strong>\u4e2d\u548c\uff1a<\/strong>\u7528\u4e2d\u548c\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u542b\u6709\u918b\u9178\u94be\u6216\u918b\u9178\uff09\u4e2d\u548c\u6eb6\u6db2\uff0c\u4f7f\u7ec6\u80de\u788e\u7247\u6c89\u6dc0\uff0c\u540c\u65f6\u4fdd\u6301\u8d28\u7c92DNA\u5728\u6eb6\u6db2\u4e2d\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-4-\u6f84\u6e05\u88c2\u89e3\u6db2\">4. \u6f84\u6e05\u88c2\u89e3\u6db2<\/h3>\n\n\n\n<p>\u901a\u8fc7\u79bb\u5fc3\u6216\u8fc7\u6ee4\u6e05\u9664\u88c2\u89e3\u6db2\uff0c\u4ee5\u53bb\u9664\u6c89\u6dc0\u6742\u8d28\u548c\u5176\u4ed6\u6c61\u67d3\u7269\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">5. \u7ed3\u5408\u8d28\u7c92DNA<\/h3>\n\n\n\n<p>\u5c06\u6f84\u6e05\u540e\u7684\u88c2\u89e3\u6db2\u901a\u8fc7\u7845\u80f6\u67f1\u6216\u5176\u4ed6DNA\u7ed3\u5408\u57fa\u8d28\u3002\u8d28\u7c92DNA\u4f1a\u7ed3\u5408\u5230\u67f1\u5b50\u4e0a\uff0c\u800c\u6742\u8d28\u5219\u6d41\u51fa\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">6. \u6d17\u6da4<\/h3>\n\n\n\n<p>\u7528\u4e00\u79cd\u6216\u591a\u79cd\u6d17\u6da4\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u542b\u6709\u4e59\u9187\u548c\u76d0\uff09\u6d17\u6da4\u67f1\u5b50\uff0c\u4ee5\u53bb\u9664\u6b8b\u7559\u7684\u6c61\u67d3\u7269\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">7. \u6d17\u8131<\/h3>\n\n\n\n<p>\u4f7f\u7528\u4f4e\u76d0\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u542b\u6709Tris-HCl\u6216\u6c34\uff09\u5c06\u8d28\u7c92DNA\u4ece\u67f1\u5b50\u4e0a\u6d17\u8131\u4e0b\u6765\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">8. \u6d53\u5ea6\u4e0e\u7eaf\u5ea6\u68c0\u6d4b<\/h3>\n\n\n\n<p>\u8d28\u7c92\u7eaf\u5316\u7684\u6700\u540e\u4e00\u6b65\u662f\u5bf9\u6d53\u5ea6\u548c\u7eaf\u5ea6\u8fdb\u884c\u8d28\u91cf\u68c0\u6d4b\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4e59\u9187\u6c89\u6dc0\uff08\u53ef\u9009\uff09\uff1a<\/strong>\u5982\u6709\u9700\u8981\uff0c\u53ef\u901a\u8fc7\u4e59\u9187\u6c89\u6dc0\u6d53\u7f29DNA\u3002<\/li>\n\n\n\n<li><strong>\u8d28\u91cf\u63a7\u5236\uff1a<\/strong>\u4f7f\u7528\u5206\u5149\u5149\u5ea6\u6cd5\uff08\u4f8b\u5982A260\/A280\u6bd4\u503c\uff09\u6d4b\u91cf\u8d28\u7c92DNA\u7684\u6d53\u5ea6\u548c\u7eaf\u5ea6\uff0c\u5e76\u8fd0\u884c\u743c\u8102\u7cd6\u51dd\u80f6\u4ee5\u68c0\u6d4b\u5176\u5b8c\u6574\u6027\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u66f4\u591a\u8d28\u7c92\u63d0\u53d6\u8d44\u6e90\">\u66f4\u591a\u8d28\u7c92\u63d0\u53d6\u8d44\u6e90<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u5b66\u4e60\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/transfection-grade-dna.html\">\u4e86\u89e3\u8d28\u7c92\u7eaf\u5ea6<\/a><\/li>\n\n\n\n<li><strong>\u63a2\u7d22\u4e0e\u8d2d\u4e70\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation.html\">\u8d28\u7c92\u63d0\u53d6<\/a><\/li>\n\n\n\n<li><strong>\u81ea\u52a8\u5316\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/automated-plasmid-purification.html\">Kingfisher PlasmidPro Maxi \u5904\u7406\u5668<\/a><\/li>\n\n\n\n<li><strong>\u63a2\u7d22\u4e0e\u8d2d\u4e70\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-mini-prep.html\">\u8d28\u7c92\u5c0f\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-midiprep.html\">\u4e2d\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a>\uff0c\u4ee5\u53ca<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-dna-maxiprep.html\">\u5927\u91cf\u63d0\u53d6\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><strong>\u8bbe\u8ba1\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/plasmid-purification-selection-tool.html\">\u8d28\u7c92\u7eaf\u5316\u9009\u62e9\u5de5\u5177<\/a><\/li>\n\n\n\n<li><strong>\u4e0b\u8f7d\u7535\u5b50\u4e66\uff1a<\/strong> <a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/plasmid-purification-techniques.html\">\u624b\u52a8\u4e0e\u81ea\u52a8\u8d28\u7c92\u7eaf\u5316\u89e3\u51b3\u65b9\u6848\u7684\u6280\u672f<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/dna-rna-purification-analysis\/plasmid-isolation\/automated-plasmid-purification.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u63a2\u7d22\u81ea\u52a8\u5316\u8d28\u7c92\u63d0\u53d6\u6280\u672f<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><em>##<\/em>&nbsp;<\/p>\n\n\n\n<p><em><em>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u5f97\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em>&nbsp;<\/em>&nbsp;&nbsp;<\/p>\n\n\n\n<p><em>\u00a9 2025 Thermo Fisher Scientific Inc. \u4fdd\u7559\u6240\u6709\u6743\u5229\u3002\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u6240\u6709\u5546\u6807\u5747\u4e3a Thermo Fisher Scientific \u53ca\u5176\u5b50\u516c\u53f8\u7684\u8d22\u4ea7\u3002<\/em>&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" 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