{"id":289,"date":"2026-02-09T09:26:40","date_gmt":"2026-02-09T09:26:40","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=289"},"modified":"2026-03-10T06:11:36","modified_gmt":"2026-03-10T06:11:36","slug":"pvdf-vs-nitrocellulose-how-to-choose-a-blot-membrane","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/pvdf-vs-nitrocellulose-how-to-choose-a-blot-membrane\/","title":{"rendered":"PVDF\u4e0e\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\uff08NC\uff09\uff1f\u5982\u4f55\u9009\u62e9\u5370\u8ff9\u819c\uff1f"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/PVDF-Header-1024x668.jpg\" alt=\"PVDF vs Nitrocellulose: How to Choose a Western Blot Transfer Membrane \" class=\"wp-image-4263\" \/><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u5bf9\u4e8e\u4efb\u4f55\u8bbe\u8ba1\u6216\u6392\u67e5\u86cb\u767d\u5370\u8ff9\uff08western blot\uff09\u5b9e\u9a8c\u7684\u4eba\u6765\u8bf4\uff0c<em>\u201cPVDF\u8fd8\u662f\u785d\u9178\u7ea4\u7ef4\u7d20\uff1f\u201d<\/em>\u8fd9\u4e2a\u95ee\u9898\u53ef\u80fd\u4e0d\u4f1a\u50cf\u6297\u4f53\u9009\u62e9\u3001\u8f6c\u819c\u65b9\u5f0f\u6216\u68c0\u6d4b\u65b9\u6cd5\u90a3\u6837\u88ab\u4f18\u5148\u8003\u8651\u3002<\/p>\n\n\n\n<p>\u4f46\u8f6c\u819c\u6750\u6599\u7684\u9009\u62e9\u4f1a\u5bf9\u7075\u654f\u5ea6\u3001\u52a8\u6001\u8303\u56f4\u548c\u6574\u4f53\u7ed3\u679c\u8d28\u91cf\u4ea7\u751f\u5de8\u5927\u5f71\u54cd\u3002\u8fd9\u662f\u4e00\u4e2a\u503c\u5f97\u4f60\u8ba4\u771f\u601d\u8003\u7684\u6b65\u9aa4\u2014\u2014\u5c24\u5176\u662f\u5f53\u4f60\u6253\u7b97\u6295\u7a3f\u671f\u520a\u65f6\u3002<\/p>\n\n\n\n<p>\u5728\u672c\u6307\u5357\u4e2d\uff0c\u6211\u4eec\u5c06\u91cd\u70b9\u4ecb\u7ecd\u5982\u4f55\u4e3a\u4f60\u7684\u86cb\u767d\u5370\u8ff9\u5b9e\u9a8c\u9009\u62e9\uff08\u5e76\u5904\u7406\uff09\u4f18\u8d28\u7684\u8f6c\u819c\u6750\u6599\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/transfer-proteins-western-blot\/membranes-transfer-buffers-western-blotting.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u4e86\u89e3PVDF\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u9009\u9879<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-yoast-seo-table-of-contents yoast-table-of-contents\"><h2>\u76ee\u5f55<\/h2><ul><li><a href=\"#h-\u5982\u4f55\u9009\u62e9pvdf\u6216\u785d\u9178\u7ea4\u7ef4\u7d20\u7b49\u8f6c\u819c\u6750\u6599\" data-level=\"2\">\u5982\u4f55\u9009\u62e9PVDF\u6216\u785d\u9178\u7ea4\u7ef4\u7d20\u7b49\u8f6c\u819c\u6750\u6599<\/a><ul><li><a href=\"#h-\u6700\u4f73\u8f6c\u819c\u6750\u6599\u53ef\u80fd\u53d6\u51b3\u4e8e\u4f60\u7684\u76ee\u6807\u86cb\u767d\" data-level=\"3\">\u6700\u4f73\u8f6c\u819c\u6750\u6599\u53ef\u80fd\u53d6\u51b3\u4e8e\u4f60\u7684\u76ee\u6807\u86cb\u767d<\/a><\/li><li><a href=\"#h-\u68c0\u6d4b\u65b9\u6cd5\u4e5f\u5f88\u91cd\u8981-\u5316\u5b66\u53d1\u5149\u8fd8\u662f\u8367\u5149\" data-level=\"3\">\u68c0\u6d4b\u65b9\u6cd5\u4e5f\u5f88\u91cd\u8981\u2014\u2014\u5316\u5b66\u53d1\u5149\u8fd8\u662f\u8367\u5149\uff1f<\/a><\/li><li><a href=\"#h-\u6709\u4e9b\u7528\u6237\u53ef\u80fd\u4e5f\u5bf9\u819c\u7684\u64cd\u4f5c\u6709\u504f\u597d\" data-level=\"3\">\u6709\u4e9b\u7528\u6237\u53ef\u80fd\u4e5f\u5bf9\u819c\u7684\u64cd\u4f5c\u6709\u504f\u597d\u3002<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u4f18\u7f3a\u70b9\" data-level=\"2\">\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u4f18\u7f3a\u70b9<\/a><ul><li><a href=\"#h-\u4f18\u70b9\" data-level=\"3\">\u4f18\u70b9<\/a><\/li><li><a href=\"#h-\u7f3a\u70b9\" data-level=\"3\">\u7f3a\u70b9<\/a><\/li><\/ul><\/li><li><a href=\"#h-pvdf\u819c\u7684\u4f18\u7f3a\u70b9\" data-level=\"2\">PVDF\u819c\u7684\u4f18\u7f3a\u70b9<\/a><ul><li><a href=\"#h-\u4f18\u70b9-0\" data-level=\"3\">\u4f18\u70b9<\/a><\/li><li><a href=\"#h-\u7f3a\u70b9-0\" data-level=\"3\">\u7f3a\u70b9<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u4f4e\u8367\u5149pvdf\u819c\u7684\u4f18\u7f3a\u70b9\" data-level=\"2\">\u4f4e\u8367\u5149PVDF\u819c\u7684\u4f18\u7f3a\u70b9<\/a><ul><li><a href=\"#h-\u4f18\u70b9-1\" data-level=\"3\">\u4f18\u70b9<\/a><\/li><li><a href=\"#h-\u7f3a\u70b9-1\" data-level=\"3\">\u7f3a\u70b9<\/a><\/li><\/ul><\/li><li><a href=\"#h-pvdf-\u8fd8\u662f\u785d\u9178\u7ea4\u7ef4\u7d20-\u54ea\u79cd\u66f4\u597d\" data-level=\"2\">PVDF \u8fd8\u662f\u785d\u9178\u7ea4\u7ef4\u7d20\uff1a\u54ea\u79cd\u66f4\u597d\uff1f<\/a><\/li><li><a href=\"#h-\u5173\u4e8e-pvdf-\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u5e38\u89c1\u95ee\u9898\" data-level=\"2\">\u5173\u4e8e PVDF \u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u5e38\u89c1\u95ee\u9898<\/a><ul><li><a href=\"#h-\u8f6c\u819c\u540e\u5982\u4f55\u4fdd\u5b58\u86cb\u767d\u5370\u8ff9\u819c-\u785d\u9178\u7ea4\u7ef4\u7d20\u548c-pvdf-\u7684\u4fdd\u5b58\u65b9\u6cd5\u6709\u533a\u522b\u5417\" data-level=\"3\">\u8f6c\u819c\u540e\u5982\u4f55\u4fdd\u5b58\u86cb\u767d\u5370\u8ff9\u819c\uff1a\u785d\u9178\u7ea4\u7ef4\u7d20\u548c PVDF \u7684\u4fdd\u5b58\u65b9\u6cd5\u6709\u533a\u522b\u5417\uff1f<\/a><\/li><li><a href=\"#h-\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548c-pvdf-\u819c\u5728\u6d3b\u5316\u6b65\u9aa4\u4e0a\u6709\u54ea\u4e9b\u4e0d\u540c\" data-level=\"3\">\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548c PVDF \u819c\u5728\u6d3b\u5316\u6b65\u9aa4\u4e0a\u6709\u54ea\u4e9b\u4e0d\u540c\uff1f<\/a><\/li><li><a href=\"#h-\u5982\u679c-pvdf-\u819c\u5e72\u4e86\u4f1a\u53d1\u751f\u4ec0\u4e48\" data-level=\"3\">\u5982\u679c PVDF \u819c\u5e72\u4e86\u4f1a\u53d1\u751f\u4ec0\u4e48\uff1f<\/a><\/li><li><a href=\"#h-\u6211\u6709-iblot-3-\u6216-iblot-2-\u80f6\u8f6c\u79fb\u4eea-\u9700\u8981\u4f7f\u7528\u54ea\u79cd\u7c7b\u578b\u7684\u819c\" data-level=\"3\">\u6211\u6709 iBlot 3 \u6216 iBlot 2 \u80f6\u8f6c\u79fb\u4eea\u3002\u9700\u8981\u4f7f\u7528\u54ea\u79cd\u7c7b\u578b\u7684\u819c\uff1f<\/a><\/li><li><a href=\"#h-pvdf-\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u7684\u8f6c\u819c\u65f6\u95f4\u6709\u533a\u522b\u5417\" data-level=\"3\">PVDF \u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u7684\u8f6c\u819c\u65f6\u95f4\u6709\u533a\u522b\u5417\uff1f<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u5173\u4e8e-pvdf-\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u66f4\u591a\u8d44\u6e90\" data-level=\"2\">\u5173\u4e8e PVDF \u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u66f4\u591a\u8d44\u6e90<\/a><\/li><\/ul><\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5982\u4f55\u9009\u62e9pvdf\u6216\u785d\u9178\u7ea4\u7ef4\u7d20\u7b49\u8f6c\u819c\u6750\u6599\">\u5982\u4f55\u9009\u62e9PVDF\u6216\u785d\u9178\u7ea4\u7ef4\u7d20\u7b49\u8f6c\u819c\u6750\u6599<\/h2>\n\n\n\n<p>Western\u8f6c\u819c\u819c\u5728\u86cb\u767d\u8d28\u5370\u8ff9\uff08Western blot\uff09\u4e2d\u81f3\u5173\u91cd\u8981\uff0c\u56e0\u4e3a\u5b83\u4eec\u5728\u7535\u6cf3\u5206\u79bb\u540e\u4e3a\u86cb\u767d\u8d28\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7a33\u5b9a\u7684\u56fa\u5b9a\u5e73\u53f0\u3002\u4e00\u65e6\u86cb\u767d\u8d28\u4ece\u51dd\u80f6\u8f6c\u79fb\u5230\u819c\u4e0a\uff0c\u7814\u7a76\u4eba\u5458\u5c31\u53ef\u4ee5\u4f7f\u7528\u7279\u5f02\u6027\u6297\u4f53\u6765\u68c0\u6d4b\u548c\u5b9a\u91cf\u76ee\u6807\u86cb\u767d\u3002<\/p>\n\n\n\n<p>\u8fd9\u4e9b\u819c\u7ecf\u8fc7\u7279\u6b8a\u8bbe\u8ba1\uff0c\u5177\u6709\u9ad8\u86cb\u767d\u7ed3\u5408\u80fd\u529b\uff0c\u5e76\u4e14\u517c\u5bb9\u591a\u79cd\u68c0\u6d4b\u65b9\u6cd5\uff0c\u5982\u5316\u5b66\u53d1\u5149\u3001\u8367\u5149\u548c\u6bd4\u8272\u5206\u6790\uff0c\u4ece\u800c\u6700\u7ec8\u63d0\u5347\u4e86\u5206\u6790\u7684\u7075\u654f\u5ea6\u548c\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n<p>\u5728Western blot\u5b9e\u9a8c\u4e2d\uff0c\u4e3b\u8981\u6709\u4e24\u79cd\u8f6c\u819c\u9009\u62e9\uff1aPVDF\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u3002\u8fd9\u4e24\u79cd\u6750\u6599\u5728\u8bb8\u591a\u76ee\u6807\u86cb\u767d\u4e0a\u90fd\u80fd\u4ea7\u751f\u7c7b\u4f3c\u4e14\u53ef\u9760\u7684\u7ed3\u679c\uff0c\u4f46\u6bcf\u79cd\u6750\u6599\u5728\u7279\u5b9a\u60c5\u51b5\u4e0b\u5404\u6709\u72ec\u7279\u4f18\u52bf\u3002<\/p>\n\n\n\n<p>\u90a3\u4e48\uff0c\u8be5\u5982\u4f55\u9009\u62e9\u5462\uff1f<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u6700\u4f73\u8f6c\u819c\u6750\u6599\u53ef\u80fd\u53d6\u51b3\u4e8e\u4f60\u7684\u76ee\u6807\u86cb\u767d\">\u6700\u4f73\u8f6c\u819c\u6750\u6599\u53ef\u80fd\u53d6\u51b3\u4e8e\u4f60\u7684\u76ee\u6807\u86cb\u767d<\/h3>\n\n\n\n<p>\u785d\u9178\u7ea4\u7ef4\u7d20\u548cPVDF\u5bf9\u5927\u591a\u6570\u86cb\u767d\u8d28\u90fd\u5177\u6709\u5f88\u5f3a\u7684\u7ed3\u5408\u4eb2\u548c\u529b\uff0c\u901a\u5e38\u4f7f\u7528\u4efb\u4e00\u7c7b\u578b\u7684\u819c\u90fd\u80fd\u83b7\u5f97\u76f8\u4f3c\u7684\u7ed3\u679c\uff08\u89c1\u56fe1\uff09\u3002\u7136\u800c\uff0c\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u7279\u5b9a\u76ee\u6807\u86cb\u767d\u53ef\u80fd\u5177\u6709\u67d0\u4e9b\u5316\u5b66\u6027\u8d28\uff08\u5982\u758f\u6c34\u6027\uff09\uff0c\u8fd9\u4f1a\u4e0e\u5176\u4e2d\u4e00\u79cd\u819c\u7684\u7ed3\u5408\u673a\u5236\u66f4\u4e3a\u5339\u914d\uff0c\u4ece\u800c\u5bfc\u81f4\u8be5\u7c7b\u578b\u819c\u5bf9\u8be5\u86cb\u767d\u8868\u73b0\u51fa\u66f4\u9ad8\u7684\u4eb2\u548c\u529b\uff0c\u4e5f\u5c31\u662f\u5bf9\u8fd9\u79cd\u819c\u8868\u73b0\u51fa\u660e\u663e\u504f\u597d\u3002<\/p>\n\n\n\n<p>\u5728\u8fd9\u4e9b\u60c5\u51b5\u4e0b\uff0c\u9009\u62e9\u54ea\u79cd\u819c\u53ef\u80fd\u4f1a\u663e\u8457\u5f71\u54cdWestern blot\u7684\u7ed3\u679c\u3002\u5982\u679c\u86cb\u767d\u4e0e\u819c\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u8f83\u5dee\uff0c\u53ef\u80fd\u4f1a\u5bfc\u81f4\u5728\u5b75\u80b2\u6216\u6d17\u6da4\u6b65\u9aa4\u4e2d\u86cb\u767d\u4ece\u819c\u4e0a\u8131\u843d\u3002\u5728\u4e25\u91cd\u65f6\u751a\u81f3\u53ef\u80fd\u5b8c\u5168\u65e0\u6cd5\u5c06\u86cb\u767d\u56fa\u5b9a\u5230\u819c\u4e0a\u3002\u5982\u679c\u4f60\u96be\u4ee5\u68c0\u6d4b\u5230\u76ee\u6807\u86cb\u767d\uff0c\u4e0d\u59a8\u5c1d\u8bd5\u66f4\u6362\u53e6\u4e00\u79cd\u7c7b\u578b\u7684\u8f6c\u819c\u4f5c\u4e3a\u8d77\u70b9\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u68c0\u6d4b\u65b9\u6cd5\u4e5f\u5f88\u91cd\u8981-\u5316\u5b66\u53d1\u5149\u8fd8\u662f\u8367\u5149\">\u68c0\u6d4b\u65b9\u6cd5\u4e5f\u5f88\u91cd\u8981\u2014\u2014\u5316\u5b66\u53d1\u5149\u8fd8\u662f\u8367\u5149\uff1f<\/h3>\n\n\n\n<p>PVDF\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u5728\u5316\u5b66\u53d1\u5149\u5e94\u7528\u4e2d\u8868\u73b0\u76f8\u4f3c\u3002\u5bf9\u4e8e<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/detect-proteins-western-blot\/western-blot-detection-reagents\/fluorescent-western-blot-detection.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u8367\u5149\u68c0\u6d4b\u65b9\u6cd5<\/a>\u7136\u800c\uff0c\u4e0d\u540c\u7c7b\u578b\u7684\u819c\u5728\u5173\u952e\u6027\u80fd\u65b9\u9762\u5b58\u5728\u5dee\u5f02\uff0c\u8fd9\u53ef\u80fd\u4f1a\u5f71\u54cd\u60a8\u7684\u9009\u62e9\u3002\u4f4e\u8367\u5149PVDF\u662f\u4e00\u79cd\u591a\u7528\u9014\u9009\u9879\uff0c\u9002\u7528\u4e8e\u6240\u6709\u68c0\u6d4b\u65b9\u6cd5\uff0c\u4f46\u5728\u8367\u5149\u68c0\u6d4b\u4e2d\u8868\u73b0\u5c24\u4e3a\u51fa\u8272\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u6709\u4e9b\u7528\u6237\u53ef\u80fd\u4e5f\u5bf9\u819c\u7684\u64cd\u4f5c\u6709\u504f\u597d\">\u6709\u4e9b\u7528\u6237\u53ef\u80fd\u4e5f\u5bf9\u819c\u7684\u64cd\u4f5c\u6709\u504f\u597d\u3002<\/h3>\n\n\n\n<p>\u819c\u7684\u6613\u64cd\u4f5c\u6027\u5bf9\u4e8e\u5e0c\u671b\u51cf\u5c11\u5de5\u827a\u6b65\u9aa4\u548c\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u7684\u7528\u6237\u6765\u8bf4\u53ef\u80fd\u662f\u4e00\u4e2a\u91cd\u8981\u56e0\u7d20\u3002\u6709\u4e9b\u819c\u5728\u4f7f\u7528\u524d\u9700\u8981\u6fc0\u6d3b\u6b65\u9aa4\uff0c\u4ee5\u786e\u4fdd\u6700\u4f73\u7ed3\u5408\u6548\u679c\u3002\u8010\u7528\u6027\u5bf9\u4e8e\u9700\u8981\u591a\u6b21\u5265\u79bb\u548c\u91cd\u590d\u63a2\u6d4b\u5370\u8ff9\u7684\u7528\u6237\u6765\u8bf4\u4e5f\u5f88\u91cd\u8981\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa639d95dae3&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa639d95dae3\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/Fig1Membranes-1024x382.jpg\" alt=\"Fig. 1 demonstrates similar western blot performance profiles of pvdf vs nitrocellulose membranes\" class=\"wp-image-4264\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><strong>\u56fe1. \u785d\u9178\u7ea4\u7ef4\u7d20\u548cPVDF\u5728\u8bb8\u591a\u76ee\u6807\u86cb\u767d\u7684western blot\u5b9e\u9a8c\u4e2d\u4ea7\u751f\u7c7b\u4f3c\u7ed3\u679c\u3002<\/strong>\u91cd\u590d\u7684western blot\u5b9e\u9a8c\u4f7f\u7528\u4e86<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/NW04122BOX?SID=srch-srp-NW04122BOX\">NuPAGE\u2122 Bis-Tris Midi\u86cb\u767d\u51dd\u80f6\uff0c4-12%<\/a>(\u76ee\u5f55\u53f7 NW04122BOX) \u8fdb\u884c\u5206\u79bb\uff0c\u5e76\u4f7f\u7528\u4e86<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/transfer-proteins-western-blot\/iblot-dry-blotting-system.html\">iBlot 3 Western Blot\u8f6c\u5370\u4eea\u5668<\/a>(\u76ee\u5f55\u53f7 IB31001)\uff0c\u4e0e\u542bNC\u6216PVDF\u7684iBlot 3\u8f6c\u5370\u57ab\uff08\u76ee\u5f55\u53f7\uff1a<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/IB33001\">IB33001<\/a>\u6216<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/IB34001\">IB34001<\/a>) \u7528\u4e8e\u86cb\u767d\u8f6c\u79fb\u3002\u819c\u88ab\u7528\u6297EGFR\u3001Calreticulin\u548cp23\u7279\u5f02\u6027\u6297\u4f53\u5b75\u80b22.5\u5c0f\u65f6\uff0c\u7136\u540e\u7528\u76f8\u5e94\u7684HRP\u4e8c\u6297\u5b75\u80b21\u5c0f\u65f6\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34076?SID=srch-hj-34076\">SuperSignal West Dura \u5ef6\u957f\u663e\u8272\u5e95\u7269<\/a>(\u76ee\u5f55\u53f7\uff1a34076)\u7528\u4e8e\u68c0\u6d4b\u3002\u5370\u8ff9\u5728<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/detect-proteins-western-blot\/western-blot-imaging-analysis\/ibright-systems\/models.html\"> iBright<\/a> FL1500 \u6210\u50cf\u7cfb\u7edf\uff08\u76ee\u5f55\u53f7\uff1aA44241\uff09\u4e0a\u6210\u50cf\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>PVDF\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u8f6c\u819c\u5728\u5408\u9002\u7684\u6761\u4ef6\u4e0b\u90fd\u53ef\u4ee5\u6210\u4e3a\u6709\u6548\u7684\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u5de5\u5177\u3002\u8ba9\u6211\u4eec\u6765\u6df1\u5165\u4e86\u89e3\u6bcf\u79cd\u819c\u7c7b\u578b\u7684\u4f18\u7f3a\u70b9\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u4f18\u7f3a\u70b9\">\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u4f18\u7f3a\u70b9<\/h2>\n\n\n\n<p>Harry Towbin\u57281979\u5e74\u9996\u6b21\u5f00\u53d1\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u6280\u672f\u65f6\u4f7f\u7528\u4e86\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\uff0c\u8fd9\u4e00\u65b9\u6cd5\u81f3\u4eca\u4ecd\u662f\u5e7f\u6cdb\u53d1\u8868\u7684\u6807\u51c6\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/NitroStructure-1-1.png\" alt=\"Structure of nitrocellulose\" class=\"wp-image-4285\" \/><figcaption class=\"wp-element-caption\">\u785d\u5316\u7ea4\u7ef4\u7d20\u7684\u7ed3\u6784<\/figcaption><\/figure>\n<\/div>\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/88018\">\u785d\u5316\u7ea4\u7ef4\u7d20\u819c<\/a>\u901a\u8fc7\u785d\u5316\u53cd\u5e94\u5236\u5907\uff0c\u5373\u7ea4\u7ef4\u7d20\u4e0e\u785d\u9178\u548c\u786b\u9178\u53cd\u5e94\u2014\u2014\u5c06\u7f9f\u57fa\u66ff\u6362\u4e3a\u785d\u57fa\u3002\u901a\u8fc7\u6c22\u952e\u3001\u6c2e\u5076\u6781\u3001\u79bb\u5b50\u548c\u758f\u6c34\u76f8\u4e92\u4f5c\u7528\uff0c\u8fd9\u4e9b\u819c\u80fd\u591f\u7ed3\u5408\u5e76\u56fa\u5b9a\u591a\u79cd\u86cb\u767d\u8d28\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u4f18\u70b9\">\u4f18\u70b9<\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u9002\u7528\u4e8e\u591a\u79cd\u7c7b\u578b\u7684\u86cb\u767d\u8d28<\/li>\n\n\n\n<li>\u86cb\u767d\u8f6c\u79fb\u65e0\u9700\u6fc0\u6d3b\u6b65\u9aa4<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u7f3a\u70b9\">\u7f3a\u70b9 <\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5728\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u6bd4PVDF\u66f4\u5bb9\u6613\u6495\u88c2<\/li>\n\n\n\n<li>\u4e0d\u517c\u5bb9\u67d0\u4e9b\u6eb6\u5242\u6216\u5f3a\u9178\u6027\u6761\u4ef6<\/li>\n\n\n\n<li>\u7531\u4e8e\u6613\u71c3\u6027\uff0c\u5728\u67d0\u4e9b\u5730\u533a\u53d7\u5230\u66f4\u4e25\u683c\u7684\u7ba1\u63a7<\/li>\n\n\n\n<li>\u7531\u4e8e\u81ea\u53d1\u8367\u5149\uff0c\u5c24\u5176\u662f\u5728\u8f83\u77ed\u6ce2\u957f\u4e0b\uff0c\u5bf9\u8367\u5149\u514d\u75ab\u5370\u8ff9\u5b9e\u9a8c\u6765\u8bf4\u5e76\u975e\u6700\u4f73\u9009\u62e9\u3002<\/li>\n<\/ol>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pvdf\u819c\u7684\u4f18\u7f3a\u70b9\">PVDF\u819c\u7684\u4f18\u7f3a\u70b9<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/88518\">\u805a\u504f\u4e8c\u6c1f\u4e59\u70ef\uff08PVDF\uff09\u819c<\/a>\u53ef\u4ee5\u5236\u9020\u4e3a\u504f\u4e8c\u6c1f\u4e59\u70ef\u7684\u5747\u805a\u7269\uff0c\u4e5f\u53ef\u4ee5\u4e0e\u5176\u4ed6\u6dfb\u52a0\u5242\u5171\u805a\u3002\u8fd9\u4f7f\u5f97\u819c\u5177\u6709\u575a\u56fa\u4e14\u7a33\u5b9a\u7684\u7279\u6027\uff0c\u5e76\u901a\u8fc7\u758f\u6c34\u76f8\u4e92\u4f5c\u7528\u7ed3\u5408\u86cb\u767d\u8d28\u3002\u6b64\u5916\uff0cPVDF\u819c\u5728\u7269\u7406\u548c\u5316\u5b66\u4e0a\u90fd\u975e\u5e38\u7a33\u5b9a\uff0c\u6613\u4e8e\u64cd\u4f5c\uff0c\u5e76\u4e14\u517c\u5bb9\u5927\u591a\u6570\u514d\u75ab\u5370\u8ff9\u5b9e\u9a8c\u6d41\u7a0b\u3002\u8fd9\u4e9b\u7279\u6027\u4f7f\u5f97PVDF\u6210\u4e3a\u8bb8\u591a\u7814\u7a76\u4eba\u5458\u9752\u7750\u7684\u53ef\u9760\u9009\u62e9\u3002\n<\/p>\n\n\n\n<p>\u7136\u800c\uff0c\u4e0e\u785d\u9178\u7ea4\u7ef4\u7d20\u4e0d\u540c\uff0cPVDF\u819c\u5728\u4f7f\u7528\u524d\u5fc5\u987b\u8fdb\u884c\u6d3b\u5316\u3002\u867d\u7136\u53ea\u9700\u77ed\u6682\u5904\u7406\uff0c\u4f46\u8fd9\u610f\u5473\u7740\u5728\u672c\u5df2\u8017\u65f6\u7684\u514d\u75ab\u5370\u8ff9\u5b9e\u9a8c\u4e2d\u53c8\u589e\u52a0\u4e86\u4e00\u6b65\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa639d95e34e&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa639d95e34e\" class=\"aligncenter size-full is-resized wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/PVDFstructure-1-1.png\" alt=\"Structure of polyvinylidene difluoride (PVDF)\" class=\"wp-image-4284\" style=\"width:300px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\">\u805a\u504f\u4e8c\u6c1f\u4e59\u70ef\uff08PVDF\uff09\u7684\u7ed3\u6784<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u4f18\u70b9-0\">\u4f18\u70b9 <\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u9002\u7528\u4e8e\u591a\u79cd\u7c7b\u578b\u7684\u86cb\u767d\u8d28<\/li>\n\n\n\n<li>\u7269\u7406\u548c\u5316\u5b66\u6027\u8d28\u7a33\u5b9a\uff0c\u4e0d\u6613\u6495\u88c2<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u7f3a\u70b9-0\">\u7f3a\u70b9  <\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u4f7f\u7528\u524d\u9700\u8981\u6fc0\u6d3b\/\u6da6\u6e7f\u6b65\u9aa4\uff0c\u9700\u7528\u7532\u9187\u6216\u4e59\u9187\u6eb6\u6db2\u5904\u7406\u3002<\/li>\n\n\n\n<li>\u81ea\u53d1\u8367\u5149\u6c34\u5e73\u9ad8\u4e8e\u785d\u9178\u7ea4\u7ef4\u7d20\uff0c\u8fd9\u4f1a\u4e25\u91cd\u5f71\u54cd\u53ef\u7528\u7684\u8367\u5149\u901a\u9053\u3001\u68c0\u6d4b\u9650\u548c\u8367\u5149\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u7684\u51c6\u786e\u6027\u3002<\/li>\n<\/ol>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa639d95e8e3&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa639d95e8e3\" class=\"aligncenter size-full is-resized wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/Fig2Membranes.jpg\" alt=\"Fig 2. Standard PVDF membranes exhibit high levels of autofluorescence\" class=\"wp-image-4273\" style=\"width:600px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><strong>\u56fe2. \u6807\u51c6PVDF\u819c\u8868\u73b0\u51fa\u8f83\u9ad8\u6c34\u5e73\u7684\u81ea\u53d1\u8367\u5149\u3002<\/strong>\u672a\u5904\u7406\u7684\u819c\u5728100%\u7532\u9187\u4e2d\u6c34\u53163\u5206\u949f\uff0c\u7136\u540e\u5728\u53bb\u79bb\u5b50\u6c34\u4e2d\u6447\u6643\u6e05\u6d175\u5206\u949f\u3002\u968f\u540e\uff0c\u5b83\u4eec\u57285\u4e2a\u4e0d\u540c\u7684\u8367\u5149\u901a\u9053\u4e2d\u4e00\u8d77\u6210\u50cf\uff0c\u4f7f\u7528<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A44241\">iBright FL1500 \u6210\u50cf\u7cfb\u7edf<\/a>(\u76ee\u5f55\u53f7\uff1aA44241)\u3002\u6bcf\u5f20\u819c\u5728\u540c\u4e00\u901a\u9053\u5185\u7684\u66dd\u5149\u65f6\u95f4\u548c\u5bf9\u6bd4\u5ea6\u5b8c\u5168\u76f8\u540c\uff0c\u5e76\u4e0e\u6807\u51c6\u86cb\u767d\u5370\u8ff9\u5e94\u7528\u4fdd\u6301\u4e00\u81f4\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4f4e\u8367\u5149pvdf\u819c\u7684\u4f18\u7f3a\u70b9\">\u4f4e\u8367\u5149PVDF\u819c\u7684\u4f18\u7f3a\u70b9<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/678000\">\u4f4e\u8367\u5149PVDF\u819c<\/a>\u5e38\u7528\u4e8e\u8367\u5149\u86cb\u767d\u5370\u8ff9\u6216\u914d\u5408\u5168\u86cb\u767d\u5b9a\u91cf\u7684\u8367\u5149\u5f52\u4e00\u5316\u65b9\u6cd5\uff0c\u4ee5\u6700\u5927\u5316\u4fe1\u566a\u6bd4\u3002<\/p>\n\n\n\n<p>\u5b83\u4eec\u65e8\u5728\u51cf\u5c11\u81ea\u53d1\u8367\u5149\u6765\u6e90\u2014\u2014\u5982\u539f\u6750\u6599\u548c\u6742\u8d28\u2014\u2014\u56e0\u6b64\u76f8\u6bd4\u785d\u9178\u7ea4\u7ef4\u7d20\u548c\u6807\u51c6PVDF\u819c\u5177\u6709\u66f4\u4f4e\u7684\u8367\u5149\u80cc\u666f\uff08\u89c1\u56fe3\uff09\u3002<\/p>\n\n\n\n<p>\u9664\u4e86\u63d0\u9ad8\u7075\u654f\u5ea6\u4e4b\u5916\uff0c\u66f4\u4f4e\u7684\u8367\u5149\u80cc\u666f\u8fd8\u4f7f\u5f97\u53ef\u4ee5\u4f7f\u7528\u66f4\u5e7f\u6cdb\u7684\u8367\u5149\u6210\u50cf\u901a\u9053\uff0c\u4ece\u800c\u8ba9\u7814\u7a76\u4eba\u5458\u80fd\u591f\u901a\u8fc7\u591a\u91cd\u68c0\u6d4b\u4ece\u6bcf\u4e00\u5f20\u86cb\u767d\u8d28\u5370\u8ff9\u819c\u4e2d\u63d0\u53d6\u663e\u8457\u66f4\u591a\u7684\u4fe1\u606f\u3002\u6700\u540e\uff0c\u8bb8\u591a\u4f4e\u8367\u5149PVDF\u819c\u5728\u5316\u5b66\u53d1\u5149\u5e94\u7528\u4e2d\u7684\u8868\u73b0\u4e0e\u6807\u51c6PVDF\u819c\u76f8\u4f3c\uff0c\u4f7f\u5176\u6210\u4e3a\u6700\u5177\u591a\u529f\u80fd\u6027\u7684\u819c\u9009\u9879\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u4f18\u70b9-1\">\u4f18\u70b9 <\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u9002\u7528\u4e8e\u591a\u79cd\u7c7b\u578b\u7684\u86cb\u767d\u8d28<\/li>\n\n\n\n<li>\u5728\u5316\u5b66\u53d1\u5149\u5e94\u7528\u4e2d\u8868\u73b0\u4e0e\u6807\u51c6PVDF\u819c\u76f8\u4f3c<\/li>\n\n\n\n<li>\u4e13\u4e3a\u964d\u4f4e\u81ea\u4f53\u8367\u5149\u800c\u8bbe\u8ba1\uff0c\u975e\u5e38\u9002\u5408\u7528\u4e8e\u8367\u5149\u68c0\u6d4b<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u7f3a\u70b9-1\">\u7f3a\u70b9 <\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u4f7f\u7528\u524d\u9700\u8981\u6fc0\u6d3b\/\u6da6\u6e7f\u6b65\u9aa4\uff0c\u9700\u7528\u7532\u9187\u6216\u4e59\u9187\u6eb6\u6db2\u5904\u7406\u3002<\/li>\n<\/ol>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa639d95ef67&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa639d95ef67\" class=\"aligncenter size-large is-resized wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/Fig3membranes-673x1024.jpg\" alt=\"Invitrogen Low Fluorescence PVDF membrane exhibits lower autofluorescence when compared to nitrocellulose and standard PVDF membranes. (PVDF vs nitrocellulose)\" class=\"wp-image-4274\" style=\"width:500px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><strong>\u56fe3\u3002<\/strong> <strong>\u4e0e\u785d\u9178\u7ea4\u7ef4\u7d20\u548c\u6807\u51c6PVDF\u819c\u76f8\u6bd4\uff0cInvitrogen\u4f4e\u8367\u5149PVDF\u819c\u8868\u73b0\u51fa\u66f4\u4f4e\u7684\u81ea\u4f53\u8367\u5149\u3002<\/strong>\u672a\u5904\u7406\u7684\u819c\u88ab\u6c34\u5316\u540e\uff0c\u5728\u517c\u5bb9\u7684\u6570\u5b57\u86cb\u767d\u8d28\u5370\u8ff9\u6210\u50cf\u7cfb\u7edf\u4e0a\u4e8e\u516d\u4e2a\u4e0d\u540c\u7684\u8367\u5149\u901a\u9053\u4e2d\u540c\u65f6\u6210\u50cf\u3002\u5728\u6bcf\u4e2a\u901a\u9053\u5185\uff0c\u5404\u79cd\u819c\u7684\u66dd\u5149\u65f6\u95f4\u548c\u5bf9\u6bd4\u5ea6\u8bbe\u7f6e\u5747\u4fdd\u6301\u4e00\u81f4\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pvdf-\u8fd8\u662f\u785d\u9178\u7ea4\u7ef4\u7d20-\u54ea\u79cd\u66f4\u597d\">PVDF \u8fd8\u662f\u785d\u9178\u7ea4\u7ef4\u7d20\uff1a\u54ea\u79cd\u66f4\u597d\uff1f<\/h2>\n\n\n\n<p>\u603b\u4e4b\uff0c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548cPVDF\u819c\u90fd\u9002\u7528\u4e8e\u86cb\u767d\u8d28\u5370\u8ff9\uff08western blot\uff09\uff0c\u4f46\u5404\u81ea\u5177\u6709\u4e0d\u540c\u7684\u4f18\u70b9\u548c\u7f3a\u70b9\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u65e0\u9700\u6fc0\u6d3b\uff0c\u4f46\u66f4\u5bb9\u6613\u6495\u88c2\u3002<\/li>\n\n\n\n<li>PVDF\u819c\u867d\u7136\u9700\u8981\u6fc0\u6d3b\uff0c\u4f46\u5177\u6709\u9ad8\u5ea6\u8010\u7528\u6027\u548c\u826f\u597d\u7684\u5316\u5b66\u517c\u5bb9\u6027\u3002<\/li>\n\n\n\n<li>\u4f4e\u8367\u5149PVDF\u662f\u6700\u5177\u591a\u529f\u80fd\u6027\u7684\u819c\u9009\u9879\uff0c\u5728\u6240\u6709\u68c0\u6d4b\u65b9\u6cd5\u4e2d\u8868\u73b0\u51fa\u8272\uff0c\u5c24\u5176\u5728\u8367\u5149\u68c0\u6d4b\u65b9\u9762\u6027\u80fd\u5353\u8d8a\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u867d\u7136\u786e\u5b9a\u9488\u5bf9\u7279\u5b9a\u76ee\u6807\u86cb\u767d\u7684\u6700\u4f73\u819c\u7c7b\u578b\u7684\u6700\u6709\u6548\u65b9\u6cd5\u662f\u6d4b\u8bd5\u86cb\u767d\u5728\u6bcf\u79cd\u819c\u4e0a\u7684\u7ed3\u5408\u60c5\u51b5\uff0c\u4f46\u8fd9\u8fc7\u7a0b\u65e2\u7e41\u7410\u53c8\u8017\u65f6\u3002\u56e0\u6b64\uff0c\u5982\u679c\u8010\u7528\u6027\u548c\u591a\u529f\u80fd\u6027\u662f\u9996\u8981\u8003\u8651\u56e0\u7d20\u2014\u2014\u7279\u522b\u662f\u5728\u8367\u5149\u68c0\u6d4b\u65b9\u9762\u2014\u2014\u4f4e\u8367\u5149PVDF\u819c\u901a\u5e38\u662f\u6700\u4f73\u9009\u62e9\u3002<\/p>\n\n\n\n<p>\u4e0b\u8868\u603b\u7ed3\u4e86\u785d\u9178\u7ea4\u7ef4\u7d20\u3001PVDF\u53ca\u4f4e\u8367\u5149PVDF\u819c\u4e4b\u95f4\u7684\u4e3b\u8981\u533a\u522b\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table aligncenter\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-center\" data-align=\"center\"><strong>\u5c5e\u6027<\/strong><\/th><th class=\"has-text-align-center\" data-align=\"center\"><strong>\u785d\u9178\u7ea4\u7ef4\u7d20<\/strong><\/th><th class=\"has-text-align-center\" data-align=\"center\">PVDF<\/th><th class=\"has-text-align-center\" data-align=\"center\"><strong>\u4f4e\u8367\u5149PVDF<\/strong><\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\">\u5316\u5b66\u53d1\u5149\u68c0\u6d4b<\/td><td class=\"has-text-align-center\" data-align=\"center\">+++<\/td><td class=\"has-text-align-center\" data-align=\"center\">+++<\/td><td class=\"has-text-align-center\" data-align=\"center\">+++<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">\u8367\u5149\u68c0\u6d4b<\/td><td class=\"has-text-align-center\" data-align=\"center\">++<\/td><td class=\"has-text-align-center\" data-align=\"center\">+<\/td><td class=\"has-text-align-center\" data-align=\"center\">+++<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">\u603b\u86cb\u767d\u5f52\u4e00\u5316<\/td><td class=\"has-text-align-center\" data-align=\"center\">++<\/td><td class=\"has-text-align-center\" data-align=\"center\">+<\/td><td class=\"has-text-align-center\" data-align=\"center\">+++<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">\u9700\u8981\u6fc0\u6d3b\u5417\uff1f<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u5426<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u662f<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u662f<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">\u7ed3\u5408\u673a\u5236<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u6c2e\u5076\u6781\u3001\u6c22\u952e\u3001\u79bb\u5b50\u548c\u758f\u6c34\u76f8\u4e92\u4f5c\u7528<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u758f\u6c34\u76f8\u4e92\u4f5c\u7528<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u758f\u6c34\u76f8\u4e92\u4f5c\u7528<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5173\u4e8e-pvdf-\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u5e38\u89c1\u95ee\u9898\">\u5173\u4e8e PVDF \u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u5e38\u89c1\u95ee\u9898<\/h2>\n\n\n\n<p>\u6211\u4eec\u7684\u7814\u53d1\u4e13\u5bb6\u5728\u4e0b\u65b9\u5206\u4eab\u4e86\u4ed6\u4eec\u5bf9\u7814\u7a76\u4eba\u5458\u6700\u5e38\u63d0\u51fa\u7684\u4e00\u4e9b\u819c\u76f8\u5173\u95ee\u9898\u7684\u56de\u7b54\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-dots\" style=\"background-color:#ee3134;color:#ee3134\" \/>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/04\/Downloadappnote-1.png\" alt=\"A pair of app notes with tips for PVDF vs nitrocellulose membranes and fluorescent western blotting and multiplexing \" class=\"wp-image-4276\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h4 class=\"wp-block-heading has-text-color has-link-color wp-elements-7821dbb5a9d0c75bbbc363f64eb68558\" id=\"h-\u9002\u7528\u4e8e\u65b0\u624b\u548c\u6709\u7ecf\u9a8c\u7528\u6237\u7684\u8f6c\u5370\u6280\u5de7\" style=\"color:#ee3134\">\u9002\u7528\u4e8e\u65b0\u624b\u548c\u6709\u7ecf\u9a8c\u7528\u6237\u7684\u8f6c\u5370\u6280\u5de7<\/h4>\n\n\n\n<p>\u4f5c\u4e3a\u65b0\u7528\u6237\u638c\u63e1\u8367\u5149\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u6280\u672f\uff0c\u6216\u901a\u8fc7\u591a\u91cd\u68c0\u6d4b\u5b66\u4e60\u5982\u4f55\u6700\u5927\u5316\u8be5\u6280\u672f\uff0c\u8bf7\u53c2\u8003\u6b64<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/fluorescent-western-blotting.html\">\u5e94\u7528\u8bf4\u660e\u4e66\u7ec4\u5408<\/a>\u3002<\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-left is-layout-flex wp-container-core-buttons-is-layout-16165478 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-background wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/fluorescent-western-blotting.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u8bbf\u95ee\u5e94\u7528\u8bf4\u660e\u4e66<\/strong><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-dots\" style=\"background-color:#ee3134;color:#ee3134\" \/>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u8f6c\u819c\u540e\u5982\u4f55\u4fdd\u5b58\u86cb\u767d\u5370\u8ff9\u819c-\u785d\u9178\u7ea4\u7ef4\u7d20\u548c-pvdf-\u7684\u4fdd\u5b58\u65b9\u6cd5\u6709\u533a\u522b\u5417\">\u8f6c\u819c\u540e\u5982\u4f55\u4fdd\u5b58\u86cb\u767d\u5370\u8ff9\u819c\uff1a\u785d\u9178\u7ea4\u7ef4\u7d20\u548c PVDF \u7684\u4fdd\u5b58\u65b9\u6cd5\u6709\u533a\u522b\u5417\uff1f <\/h3>\n\n\n\n<p>\u7406\u60f3\u60c5\u51b5\u4e0b\uff0c\u8f6c\u5370\u540e\u5e94\u7acb\u5373\u8fdb\u884c\u514d\u75ab\u68c0\u6d4b\u3002\u4f46\u5982\u679c\u9700\u8981\u5c06\u819c\u4fdd\u5b58\u4ee5\u4fbf\u4e4b\u540e\u8fdb\u884c\u514d\u75ab\u63a2\u9488\u5b9e\u9a8c\uff0c\u5efa\u8bae\u91c7\u53d6\u4ee5\u4e0b\u6b65\u9aa4\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u51b2\u6d17\uff1a<\/strong>\u7528\u84b8\u998f\u6c34\u5feb\u901f\u51b2\u6d17\u819c\uff0c\u4ee5\u53bb\u9664\u6b8b\u7559\u7684\u8f6c\u5370\u7f13\u51b2\u6db2\u3002<\/li>\n\n\n\n<li><strong>\u5e72\u71e5\uff1a<\/strong>\u5c06\u819c\u7247\u653e\u5728\u5e72\u51c0\u7684\u6ee4\u7eb8\u4e0a\uff0c\u5e76\u8ba9\u5176\u5b8c\u5168\u81ea\u7136\u98ce\u5e72\u3002\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548cPVDF\u819c\u5728\u5e72\u71e5\u72b6\u6001\u4e0b\u975e\u5e38\u7a33\u5b9a\uff0c\u53ef\u4ee5\u957f\u671f\u4fdd\u5b58\u3002<\/li>\n\n\n\n<li><strong>\u50a8\u5b58\uff1a<\/strong>\u5c06\u5e72\u71e5\u540e\u7684\u819c\u7247\u50a8\u5b58\u5728\u5e72\u51c0\u3001\u5e72\u71e5\u7684\u5bb9\u5668\u4e2d\uff0c\u6216\u5939\u5728\u6ee4\u7eb8\u4e4b\u95f4\uff0c\u7f6e\u4e8e\u5ba4\u6e29\u4e0b\u3002\u786e\u4fdd\u819c\u7247\u4fdd\u6301\u5e73\u6574\uff0c\u4ee5\u907f\u514d\u51fa\u73b0\u6298\u75d5\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u5e72\u71e5\u7684PVDF\u819c\u5728\u5c01\u95ed\u548c\u7528\u4e00\u6297\u5b75\u80b2\u524d\u9700\u8981\u5148\u7528100%\u7532\u9187\u6216\u4e59\u9187\u91cd\u65b0\u6c34\u5316\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548c-pvdf-\u819c\u5728\u6d3b\u5316\u6b65\u9aa4\u4e0a\u6709\u54ea\u4e9b\u4e0d\u540c\">\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548c PVDF \u819c\u5728\u6d3b\u5316\u6b65\u9aa4\u4e0a\u6709\u54ea\u4e9b\u4e0d\u540c\uff1f<\/h3>\n\n\n\n<p>PVDF\u819c\u9700\u8981\u901a\u8fc7\u6d78\u6ce1\u5728100%<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/423950010\">\u7532\u9187<\/a>\u6216<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/T038181000\">\u4e59\u9187<\/a>\u4e2d3\u5206\u949f\u8fdb\u884c\u9884\u6d3b\u5316\uff0c\u7136\u540e\u5feb\u901f\u7528\u53bb\u79bb\u5b50\u6c34\u51b2\u6d172\u5728\u4f7f\u7528\u524d\u9700\u8981\u8fdb\u884c\u6fc0\u6d3b\u6b65\u9aa4\u3002\u8fd9\u4e00\u6b65\u662f\u5fc5\u8981\u7684\uff0c\u56e0\u4e3aPVDF\u5177\u6709\u9ad8\u5ea6\u758f\u6c34\u6027\uff0c\u5bfc\u81f4\u5176\u96be\u4ee5\u4e0e\u6c34\u53d1\u751f\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n\n\n\n<p>\u5728\u6fc0\u6d3b\u6b65\u9aa4\u4e2d\uff0c\u7532\u9187\u6216\u4e59\u9187\u4f5c\u4e3a\u6da6\u6e7f\u5242\uff0c\u6253\u7834\u975e\u6781\u6027\u819c\u4e0e\u6781\u6027\u7f13\u51b2\u6db2\u4e4b\u95f4\u7684\u8868\u9762\u5f20\u529b\uff0c\u4f7f\u5b83\u4eec\u80fd\u591f\u76f8\u4e92\u4f5c\u7528\u3002\u7532\u9187\u8fd8\u53ef\u4ee5\u589e\u5f3a\u819c\u7684\u758f\u6c34\u4f5c\u7528\uff0c\u4f7f\u8f6c\u79fb\u7684\u86cb\u767d\u8d28\u80fd\u591f\u7d27\u5bc6\u7ed3\u5408\u5230\u819c\u4e0a\u3002<\/p>\n\n\n\n<p>\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u901a\u5e38\u53ea\u9700\u5728\u7f13\u51b2\u6db2\u6216\u6c34\u4e2d\u7b80\u5355\u6da6\u6e7f\u5373\u53ef\u4f7f\u7528\uff0c\u65e0\u9700\u7532\u9187\u5904\u7406\u3002\u8fd9\u4e9b\u5dee\u5f02\u610f\u5473\u7740PVDF\u819c\u9700\u8981\u989d\u5916\u7684\u6fc0\u6d3b\u6b65\u9aa4\uff0c\u800c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u5219\u66f4\u5bb9\u6613\u51c6\u5907\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u5982\u679c-pvdf-\u819c\u5e72\u4e86\u4f1a\u53d1\u751f\u4ec0\u4e48\">\u5982\u679c PVDF \u819c\u5e72\u4e86\u4f1a\u53d1\u751f\u4ec0\u4e48\uff1f<\/h3>\n\n\n\n<p>\u5982\u679cPVDF\u819c\u5728\u5c01\u95ed\u4e4b\u524d\u5e72\u71e5\uff0c\u53ef\u4ee5\u6309\u7167\u4e0a\u8ff0\u65b9\u6cd5\u91cd\u65b0\u6c34\u5316\uff0c\u7136\u540e\u518d\u8fdb\u884c\u5c01\u95ed\u6b65\u9aa4\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u8fd9\u4e0d\u4f1a\u5f71\u54cd\u86cb\u767d\u8d28\u7ed3\u5408\u3002\u5efa\u8bae\u907f\u514d\u5728\u5316\u5b66\u53d1\u5149\u5e95\u7269\u68c0\u6d4b\u524d\u7acb\u5373\u5e72\u71e5\u3002\u5982\u679c\u91c7\u7528\u8367\u5149\u68c0\u6d4b\u65b9\u6cd5\uff0c\u5927\u591a\u6570\u5370\u8ff9\u901a\u5e38\u53ef\u4ee5\u76f4\u63a5\u5e72\u71e5\u6210\u50cf\u800c\u65e0\u9700\u91cd\u65b0\u6da6\u6e7f\uff0c\u4f46\u67d0\u4e9b\u8367\u5149\u67d3\u6599\u5bb9\u6613\u964d\u89e3\uff0c\u800c\u8fd9\u79cd\u964d\u89e3\u4f1a\u56e0\u819c\u5e72\u71e5\u800c\u52a0\u901f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u6211\u6709-iblot-3-\u6216-iblot-2-\u80f6\u8f6c\u79fb\u4eea-\u9700\u8981\u4f7f\u7528\u54ea\u79cd\u7c7b\u578b\u7684\u819c\">\u6211\u6709 iBlot 3 \u6216 iBlot 2 \u80f6\u8f6c\u79fb\u4eea\u3002\u9700\u8981\u4f7f\u7528\u54ea\u79cd\u7c7b\u578b\u7684\u819c\uff1f <\/h3>\n\n\n\n<p>iBlot 3\u7cfb\u7edf\u7528\u4e8e\u86cb\u767d\u8f6c\u79fb\u65f6\u53ef\u4f7f\u7528\u785d\u9178\u7ea4\u7ef4\u7d20\u3001PVDF\u548c\u4f4e\u8367\u5149PVDF\u819c\uff0c\u800ciBlot 2\u4ec5\u4f7f\u7528\u785d\u9178\u7ea4\u7ef4\u7d20\u548cPVDF\u3002iBlot\u8f6c\u79fb\u5806\u53e0\u5c06\u8fd9\u4e9b\u819c\u76f4\u63a5\u96c6\u6210\u4e8e\u5806\u53e0\u4e2d\u6216\u5355\u72ec\u5305\u88c5\uff0c\u4ee5\u6700\u5927\u7a0b\u5ea6\u63d0\u4f9b\u4fbf\u5229\uff0c\u540c\u65f6\u4fdd\u6301\u4f18\u5f02\u7684\u8f6c\u79fb\u6548\u7387\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-pvdf-\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u7684\u8f6c\u819c\u65f6\u95f4\u6709\u533a\u522b\u5417\">PVDF 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\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u86cb\u767d\u8d28\u5370\u8ff9\u8f6c\u819c\u6d41\u7a0b\u53ca\u76f8\u5173\u5185\u5bb9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u6982\u8ff0\uff1a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/western-blot-transfer-methods.html#:~:text=The%20most%20common%20immobilization%20membranes,High%20binding%20capacity\">\u86cb\u767d\u8d28\u5370\u8ff9\u8f6c\u819c\u65b9\u6cd5<\/a><\/li>\n\n\n\n<li>\u5e94\u7528\u8bf4\u660e \u2013<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/fluorescent-western-blotting.html\">\u57fa\u7840\u8367\u5149\u86cb\u767d\u8d28\u5370\u8ff9\u6280\u672f\u4e0e\u591a\u91cd\u68c0\u6d4b<\/a><\/li>\n\n\n\n<li>\u6982\u8ff0\uff1a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/fluorescent-western-blotting.html\">\u8367\u5149\u86cb\u767d\u8d28\u5370\u8ff9\u6280\u672f<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/transfer-proteins-western-blot\/membranes-transfer-buffers-western-blotting.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u4e86\u89e3PVDF\u548c\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u7684\u9009\u9879<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>##<\/p>\n\n\n\n<p><em>\u00a9 2025 Thermo Fisher Scientific Inc. \u7248\u6743\u6240\u6709\u3002\u6240\u6709\u5546\u6807\u5747\u5f52 Thermo Fisher Scientific \u53ca\u5176\u5b50\u516c\u53f8\u6240\u6709\uff0c\u9664\u975e\u53e6\u6709\u8bf4\u660e\u3002<\/em>&nbsp;<em>\u5df2\u6307\u5b9a\u3002<\/em><\/p>\n\n\n\n<p><em><em>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u5f97\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image 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