{"id":340,"date":"2026-02-25T02:57:07","date_gmt":"2026-02-25T02:57:07","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=340"},"modified":"2026-03-10T06:57:07","modified_gmt":"2026-03-10T06:57:07","slug":"the-essential-guide-to-hepes-in-cell-culture","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/the-essential-guide-to-hepes-in-cell-culture\/","title":{"rendered":"HEPES\u5728\u7ec6\u80de\u57f9\u517b\u4e2d\u7684\u4f5c\u7528\u6307\u5357"},"content":{"rendered":"\n<p>\u7f13\u51b2\u4f5c\u7528\u662f\u6307\u6eb6\u6db2\u62b5\u6297pH\u53d8\u5316\u7684\u80fd\u529b\u3002\u7f13\u51b2\u6db2\u901a\u5e38\u7531\u4e00\u5bf9\u5f31\u9178\u53ca\u5176\u5171\u8f6d\u78b1\uff0c\u6216\u5f31\u78b1\u53ca\u5176\u5171\u8f6d\u9178\u7ec4\u6210\uff0c\u5b83\u4eec\u534f\u540c\u4f5c\u7528\u4ee5\u4e2d\u548c\u52a0\u5165\u7684\u9178\u6216\u78b1\u3002\u4e3a\u4e86\u51cf\u8f7b\u57f9\u517b\u7bb1\u4e2d\u9ad8CO2\u6c34\u5e73\u4ea7\u751f\u7684\u9178\u6027\uff0c\u4f1a\u5728<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-media\" target=\"_blank\" rel=\"noreferrer noopener\">\u7ec6\u80de\u57f9\u517b\u57fa<\/a>\u4e2d\u52a0\u5165\u4ee5\u78b3\u9178\u6c22\u94a0\u4e3a\u57fa\u7840\u6216HEPES\u7f13\u51b2\u5242\u6765\u5b9e\u73b0\u3002\u7ef4\u6301\u7a33\u5b9a\u7684pH\u503c\u5bf9\u4e8e\u7ec6\u80de\u7684\u6700\u4f73\u529f\u80fd\u548c\u751f\u957f\u81f3\u5173\u91cd\u8981\u3002\u8fd9\u79cd\u5fc5\u8981\u7684\u7f13\u51b2\u4f5c\u7528\u901a\u8fc7\u5728\u60a8\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-media\" target=\"_blank\" rel=\"noreferrer noopener\">\u7ec6\u80de\u57f9\u517b\u57fa<\/a>\u4e2d\u52a0\u5165\u4ee5\u78b3\u9178\u6c22\u94a0\u4e3a\u57fa\u7840\u6216HEPES\u7f13\u51b2\u5242\u6765\u5b9e\u73b0\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662fhepes\">\u4ec0\u4e48\u662fHEPES\uff1f<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15630080?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-hepes\" target=\"_blank\" rel=\"noreferrer noopener\">HEPES<\/a>\uff08N-2-\u7f9f\u4e59\u57fa\u54cc\u55ea-N-2-\u4e59\u78fa\u9178\uff09\u662f\u4e00\u79cd\u4e24\u6027\u6709\u673a\u5316\u5b66\u7f13\u51b2\u5242\u3002\u5b83\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\uff0c\u4ee5\u7ef4\u6301\u7a33\u5b9a\u7684pH\u6c34\u5e73\u3002\u867d\u7136\u7531\u4e8e\u8425\u517b\u4f18\u52bf\uff0c\u78b3\u9178\u6c22\u94a0\u662f\u7ec6\u80de\u57f9\u517b\u7f13\u51b2\u6db2\u4e2d\u6700\u5e38\u7528\u7684\u6210\u5206\uff0c\u4f46\u5728\u751f\u7406pH\u6761\u4ef6\u4e0b\uff0c\u78b3\u9178\u6c22\u94a0\u7684\u7f13\u51b2\u80fd\u529b\u4f4e\u4e8eHEPES\u7f13\u51b2\u5242\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-hepes\u7684\u7528\u9014\u662f\u4ec0\u4e48\">HEPES\u7684\u7528\u9014\u662f\u4ec0\u4e48\uff1f<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15630080?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-hepes\" target=\"_blank\" rel=\"noreferrer noopener\">HEPES<\/a>\uff08N-2-\u7f9f\u4e59\u57fa\u54cc\u55ea-N-2-\u4e59\u78fa\u9178\uff09\u662f\u4e00\u79cd\u4e24\u6027\u6709\u673a\u5316\u5b66\u7f13\u51b2\u5242\u3002\u5b83\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\uff0c\u4ee5\u7ef4\u6301\u7a33\u5b9a\u7684pH\u6c34\u5e73\u3002\u867d\u7136\u7531\u4e8e\u8425\u517b\u4f18\u52bf\uff0c\u78b3\u9178\u6c22\u94a0\u662f\u7ec6\u80de\u57f9\u517b\u7f13\u51b2\u6db2\u4e2d\u6700\u5e38\u7528\u7684\u6210\u5206\uff0c\u4f46\u5728\u751f\u7406pH\u6761\u4ef6\u4e0b\uff0c\u78b3\u9178\u6c22\u94a0\u7684\u7f13\u51b2\u80fd\u529b\u4f4e\u4e8eHEPES\u7f13\u51b2\u5242\u3002\u88ab\u8ba4\u4e3a\u662f\u751f\u7269\u7814\u7a76\u4e2d\u6700\u4f18\u79c0\u7684\u901a\u7528\u7f13\u51b2\u6db2\u4e4b\u4e00\u3002\u8fd9\u4e00\u70b9\u5728\u7ec6\u80de\u57f9\u517b\u9700\u8981\u957f\u65f6\u95f4\u5728CO2\u5b75\u7bb1\u5916\u64cd\u4f5c\u65f6\u5c24\u4e3a\u91cd\u8981\u3002\u5411\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a010\u201325 mM HEPES\u53ef\u4ee5\u63d0\u4f9b\u989d\u5916\u7684\u7f13\u51b2\u80fd\u529b\uff0c\u6709\u52a9\u4e8e\u786e\u4fddpH\u503c\u4fdd\u6301\u7a33\u5b9a\uff0c\u4fdd\u62a4\u7ec6\u80de\u514d\u53d7pH\u6ce2\u52a8\u5f71\u54cd\uff0c\u5426\u5219\u8fd9\u4e9b\u6ce2\u52a8\u53ef\u80fd\u4f1a\u5f71\u54cd\u7ec6\u80de\u7684\u5b58\u6d3b\u548c\u529f\u80fd\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e3a\u4ec0\u4e48\u5728\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u52a0\u5165hepes\">\u4e3a\u4ec0\u4e48\u5728\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u52a0\u5165HEPES\uff1f<\/h2>\n\n\n\n<p>HEPES\u7684\u4e3b\u8981\u4f5c\u7528\u662f\u5728\u751f\u7406pH\u8303\u56f4\uff087.2\u81f37.6\uff09\u5185\u4f5c\u4e3a\u7f13\u51b2\u5242\uff0c\u786e\u4fdd\u7ec6\u80de\u5b58\u6d3b\u7684\u6700\u4f73\u6761\u4ef6\u3002HEPES\u5177\u6709\u5316\u5b66\u548c\u9176\u5b66\u7a33\u5b9a\u6027\uff0c\u5e76\u4e14\u4e0d\u53ef\u900f\u8fc7\u7ec6\u80de\u819c\uff0c\u56e0\u6b64\u9002\u5408\u6dfb\u52a0\u5230\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u3002<\/p>\n\n\n\n<p>HEPES\u5728\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\u7684\u6d53\u5ea6\u53ef\u80fd\u4ece10 mM\u523025 mM\u4e0d\u7b49\u3002<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media\/dmem.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-dmem\" target=\"_blank\" rel=\"noreferrer noopener\">Gibco\u202fDMEM<\/a>\u4e2d\uff0cHEPES\u7684\u6d53\u5ea6\u4e3a25 mM\uff0c\u800c\u5728<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media\/dmem-f12.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-dmemf12\" target=\"_blank\" rel=\"noreferrer noopener\">Gibco\u202fDMEM\/F-12<\/a> \u4e2d\uff0c\u5176\u6d53\u5ea6\u4e3a15 mM\u3002\u6700\u5e38\u7528\u7684\u6d53\u5ea6\u662f25 mM\u3002\u202f<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9009\u62e9hepes\u4f5c\u4e3a\u60a8\u7814\u7a76\u7684\u7406\u7531\">\u9009\u62e9HEPES\u4f5c\u4e3a\u60a8\u7814\u7a76\u7684\u7406\u7531<\/h2>\n\n\n\n<p>Gibco HEPES\uff084-(2-\u7f9f\u4e59\u57fa)-1-\u54cc\u55ea\u4e59\u78fa\u9178\uff09\u88ab\u5f52\u7c7b\u4e3a\u201cGood\u201d\u7f13\u51b2\u6db2\uff0c\u8fd9\u4e00\u540d\u79f0\u6e90\u81ea1966\u5e74Norman Good\u535a\u58eb\u53ca\u5176\u540c\u4e8b\u6240\u63cf\u8ff0\u7684\u4e00\u7cfb\u5217\u7f13\u51b2\u6db2\uff08Good\u7b49\u4eba\uff0c<em>\u751f\u7269\u5316\u5b66<\/em>1966\u5e74\uff09\u3002<br><br>\u50cfHEPES\u8fd9\u6837\u7684\u201cGood\u7f13\u51b2\u6db2\u201d\u5177\u6709\u4ee5\u4e0b\u7279\u6027\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>pKa\u503c\u4ecb\u4e8e6.0\u52308.0\u4e4b\u95f4<\/li>\n\n\n\n<li>\u9ad8\u6eb6\u89e3\u5ea6<\/li>\n\n\n\n<li>\u5bf9\u7ec6\u80de\u819c\u4e0d\u53ef\u6e17\u900f\u6027<\/li>\n\n\n\n<li>\u5bf9\u751f\u5316\u53cd\u5e94\u5f71\u54cd\u6709\u9650<\/li>\n\n\n\n<li>\u6781\u4f4e\u7684\u53ef\u89c1\u5149\u548c\u7d2b\u5916\u5149\u5438\u6536<\/li>\n\n\n\n<li>\u5316\u5b66\u548c\u9176\u5b66\u7a33\u5b9a<\/li>\n\n\n\n<li>\u6613\u4e8e\u5236\u5907<\/li>\n<\/ul>\n\n\n\n<p>\u603b\u4e4b\uff0cHEPES\u662f\u4e00\u79cd\u53ef\u9760\u4e14\u9ad8\u6548\u7684\u7f13\u51b2\u5242\uff0c\u80fd\u591f\u589e\u5f3a\u7ec6\u80de\u57f9\u517b\u5728\u5404\u79cd\u7814\u7a76\u5e94\u7528\u4e2d\u7684\u7a33\u5b9a\u6027\u548c\u5b58\u6d3b\u7387\u3002\u5176\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u7ef4\u6301\u7ec6\u80de\u57f9\u517b\u57fa\u751f\u7406pH\u503c\u7684\u6781\u4f73\u9009\u62e9\uff0c\u5c24\u5176\u9002\u7528\u4e8e\u9700\u8981\u5728CO2\u57f9\u517b\u7bb1\u5916\u957f\u65f6\u95f4\u64cd\u4f5c\u7684\u60c5\u51b5\u3002<\/p>\n\n\n\n<p><strong>\u76f8\u5173\u5185\u5bb9\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u4f7f\u7528HEPES\u57f9\u517b\u57fa\uff0c\u5c3d\u5728<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-media\/gibco-media-formulation-tool.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-MedTool\" target=\"_blank\" rel=\"noreferrer noopener\">Gibco \u57f9\u517b\u57fa\u9009\u62e9\u5de5\u5177<\/a><\/li>\n\n\n\n<li>\u901a\u8fc7\u641c\u7d22\u60a8\u611f\u5174\u8da3\u7684\u7ec6\u80de\u7cfb\uff0c\u5229\u7528\u4ee5\u4e0b\u5de5\u5177\u67e5\u627e\u7ec6\u80de\u57f9\u517b\u8d44\u6e90\u548c\u5efa\u8bae\uff1a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-workflow-solutions\/cell-culture-select-tool.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-CCST\" target=\"_blank\" rel=\"noreferrer noopener\">Cell Culture Select Tool\uff08\u7ec6\u80de\u57f9\u517b\u9009\u62e9\u5de5\u5177\uff09<\/a><\/li>\n\n\n\n<li>\u9605\u8bfb\u66f4\u591a\u5173\u4e8e\u6211\u4eec\u7684\u57f9\u517b\u57fa\u3001FBS\u548c\u5851\u6599\u5236\u54c1\u5982\u4f55\u7ecf\u8fc7\u9a8c\u8bc1\uff0c\u4ee5\u786e\u4fdd\u5353\u8d8a\u4ea7\u54c1\u6027\u80fd\uff0c\u4ece\u800c\u8ba9\u60a8\u80fd\u591f<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/cell-culture-workflow-solutions.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-CwC\" target=\"_blank\" rel=\"noreferrer noopener\">\u81ea\u4fe1\u5730\u8fdb\u884c\u57f9\u517b<\/a>\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u6765\u6e90\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15630080?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-hepes\" target=\"_blank\" rel=\"noreferrer noopener\">Gibco HEPES\u4ea7\u54c1\u9875\u9762<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-culture\/mammalian-cell-culture\/reagents\/balanced-salt-solutions.html?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-BSS\" target=\"_blank\" rel=\"noreferrer noopener\">\u7f13\u51b2\u76d0\u6eb6\u6db2<\/a>\u4ea7\u54c1\u4fe1\u606f\u8bf4\u660e\u4e66<\/li>\n\n\n\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/5942950\/?cid=bid_clb_cce_r01_co_cp1610_pgm1522_col000000_0so_blg_il_awa_og_s00_HEPESBlg-Ref1\" target=\"_blank\" rel=\"noreferrer noopener\">\u7ec6\u80de\u57f9\u517b\u57fa\u8865\u5145\u5242<\/a><\/li>\n\n\n\n<li>Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RM. \u7528\u4e8e\u751f\u7269\u7814\u7a76\u7684\u6c22\u79bb\u5b50\u7f13\u51b2\u5242\u3002\u751f\u7269\u5316\u5b66\u6742\u5fd7\u30021966\u5e742\u6708\uff1b5(2):467-77\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u00a9 2025 Thermo Fisher Scientific Inc. \u4fdd\u7559\u6240\u6709\u6743\u5229\u3002\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u6240\u6709\u5546\u6807\u5747\u4e3aThermo Fisher Scientific\u53ca\u5176\u5b50\u516c\u53f8\u7684\u8d22\u4ea7\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, 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