{"id":525,"date":"2026-02-27T02:18:23","date_gmt":"2026-02-27T02:18:23","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=525"},"modified":"2026-03-09T05:55:38","modified_gmt":"2026-03-09T05:55:38","slug":"what-is-digital-pcr","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/what-is-digital-pcr\/","title":{"rendered":"\u4ec0\u4e48\u662f\u6570\u5b57Digital PCR\uff1f\u2014\u2014\u4e86\u89e3dPCR\u7684\u4f18\u52bf"},"content":{"rendered":"\n<p>\u6570\u5b57PCR\u662f\u4e00\u79cd\u4e13\u95e8\u7528\u4e8e\u6838\u9178\u68c0\u6d4b\u548c\u5b9a\u91cf\u7684\u65b9\u6cd5\uff0c\u901a\u8fc7\u7edf\u8ba1\u5b66\u65b9\u6cd5\u4f30\u7b97\u5206\u5b50\u7684\u7edd\u5bf9\u6570\u91cf\u3002\u6570\u5b57PCR\uff08dPCR\uff09\u4f7f\u7528\u4e0eqPCR\u76f8\u540c\u7684\u57fa\u672c\u5316\u5b66\u53cd\u5e94\uff0c\u4f46\u4e0eqPCR\u6570\u636e\u4e0d\u540c\uff0cdPCR\u7684\u6570\u636e\u662f\u5728\u53cd\u5e94\u6df7\u5408\u7269\u7ec8\u70b9\u65f6\u6536\u96c6\u7684\u3002\u5728\u6269\u589e\u4e4b\u524d\uff0c\u7531\u6838\u9178\u3001\u5f15\u7269\u3001\u63a2\u9488\u548c\u4e3b\u6df7\u5408\u6db2\u7ec4\u6210\u7684\u5927\u4f53\u79efPCR\u53cd\u5e94\u4f1a\u88ab<a title=\"Microfluidic Array Plate Technology - Powerfully Simple Digital PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/microfluidic-array-plate-technology.html\" target=\"_blank\" rel=\"noopener\">\u5206\u5272\u6210\u6210\u5343\u4e0a\u4e07\u4e2a\u7eb3\u5347\u7ea7\u5fae\u53cd\u5e94\u4f53\u7cfb<\/a>\u3002\u7531\u4e8e\u8fd9\u79cd\u6570\u5b57\u5316\u8fc7\u7a0b\u5c06PCR\u6df7\u5408\u6db2\u5206\u914d\u5230\u5982\u6b64\u591a\u7684\u5fae\u53cd\u5e94\u4e2d\uff0c\u6bcf\u4e2a\u5fae\u53cd\u5e94\u5b9e\u9645\u4e0a\u53ea\u4f1a\u5305\u542b\u4e00\u4e2a\u3001\u96f6\u4e2a\u6216\u5c11\u91cf\u76ee\u6807\u6838\u9178\u5206\u5b50\u3002\u968f\u540e\u5c06\u8fd9\u4e9b\u9694\u79bb\u7684\u5fae\u53cd\u5e94\u8fdb\u884c\u6269\u589e\uff0c\u5e76\u5728\u70ed\u5faa\u73af\u7ed3\u675f\u540e\u4ece\u6bcf\u4e2a\u5fae\u53cd\u5e94\u4e2d\u6536\u96c6\u6570\u636e\u3002\u4e0d\u542b\u76ee\u6807\u5206\u5b50\u7684\u5fae\u53cd\u5e94\u5728\u6269\u589e\u540e\u4e0d\u4f1a\u663e\u793a\u8367\u5149\uff0c\u800c\u542b\u6709\u76ee\u6807\u5206\u5b50\u7684\u5219\u4f1a\u663e\u793a\u6269\u589e\u540e\u7684\u8367\u5149\u4fe1\u53f7\u3002<br><br>\n\n\u5b9e\u9645\u4e0a\uff0c\u539f\u59cb\u53cd\u5e94\u88ab\u8f6c\u53d8\u4e3a\u8bb8\u591a\u4e8c\u5143\uff08\u6709\/\u65e0\uff09\u53cd\u5e94\u3002\u5728\u7edf\u8ba1\u9633\u6027\u5fae\u53cd\u5e94\u6570\u540e\uff0c\u53ef\u4ee5\u5229\u7528\u7b80\u5355\u7684\u7edf\u8ba1\u65b9\u6cd5\u76f4\u63a5\u786e\u5b9a\u76ee\u6807\u5206\u5b50\u7684\u201c\u7edd\u5bf9\u201d\u6570\u91cf\uff0c\u800c\u65e0\u9700\u901a\u8fc7\u4e0e\u5df2\u77e5\u6d53\u5ea6\u6807\u51c6\u54c1\u6bd4\u8f83\u6765\u4f30\u7b97\u3002\u8fd9\u9879\u6280\u672f\u4e3a\u7edd\u5bf9\u5b9a\u91cf\u548c\u7a00\u6709\u7b49\u4f4d\u57fa\u56e0\u68c0\u6d4b\u63d0\u4f9b\u4e86qPCR\u4e4b\u5916\u7684\u53e6\u4e00\u79cd\u9009\u62e9\uff0c\u5e76\u4e14\u4e0d\u4f9d\u8d56\u4e8e\u6269\u589e\u5faa\u73af\u6570\u6765\u5224\u65ad\u6bcf\u4e2a\u6837\u672c\u521d\u59cb\u6a21\u677f\u6838\u9178\u7684\u542b\u91cf\u3002<br><br>\n\u8fc4\u4eca\u4e3a\u6b62\uff0cqPCR\u4e00\u76f4\u662f\u4e00\u79cd\u529f\u80fd\u5f3a\u5927\u4e14\u7075\u654f\u7684\u57fa\u56e0\u5206\u6790\u6280\u672f\uff0c\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u5404\u79cd\u9886\u57df\u3002\u6b63\u5982\u5176\u540d\u79f0\u6240\u793a\uff0cqPCR\u662f\u5728\u6269\u589e\u8fc7\u7a0b\u4e2d\u5b9e\u65f6\u6d4b\u91cfPCR\u4ea7\u7269\uff0c\u800c\u4f20\u7edfPCR\u5219\u662f\u5728\u6574\u4e2a\u53cd\u5e94\u5b8c\u6210\u540e\u624d\u6536\u96c6\u7ed3\u679c\uff0c\u56e0\u6b64\u65e0\u6cd5\u786e\u5b9a\u8d77\u59cb\u6838\u9178\u6d53\u5ea6\u3002qPCR\u8fdb\u5165\u5e02\u573a\u5f7b\u5e95\u6539\u53d8\u4e86\u57fa\u4e8ePCR\u7684DNA\u548cRNA\u5b9a\u91cf\u65b9\u5f0f\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6570\u5b57-pcr-vs-\u5b9e\u65f6\u8367\u5149\u5b9a\u91cf-pcr-vs-\u4f20\u7edf-pcr\">\u6570\u5b57 PCR vs. \u5b9e\u65f6\u8367\u5149\u5b9a\u91cf PCR vs. \u4f20\u7edf PCR<\/h2>\n\n\n\n<p>\u8fc4\u4eca\u4e3a\u6b62\uff0cqPCR\u5df2\u6210\u4e3a\u4e00\u79cd\u5f3a\u5927\u4e14\u7075\u654f\u7684\u57fa\u56e0\u5206\u6790\u6280\u672f\uff0c\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u5404\u79cd\u9886\u57df\u3002\u987e\u540d\u601d\u4e49\uff0cqPCR\u80fd\u591f\u5b9e\u65f6\u76d1\u6d4bPCR\u6269\u589e\u8fc7\u7a0b\uff0c\u800c\u4f20\u7edfPCR\u5219\u662f\u5728\u53cd\u5e94\u7ed3\u675f\u540e\u6536\u96c6\u7ed3\u679c\uff0c\u56e0\u6b64\u65e0\u6cd5\u786e\u5b9a\u6838\u9178\u7684\u8d77\u59cb\u6d53\u5ea6\u3002qPCR\u8fdb\u5165\u5e02\u573a\u540e\uff0c\u5f7b\u5e95\u6539\u53d8\u4e86\u57fa\u4e8ePCR\u7684DNA\u548cRNA\u5b9a\u91cf\u65b9\u6cd5\u3002<\/p>\n\n\n\n<p>\u6570\u5b57PCR\u662f\u4e00\u79cd\u8f83\u65b0\u7684\u6838\u9178\u68c0\u6d4b\u4e0e\u5b9a\u91cf\u65b9\u6cd5\uff0c\u901a\u8fc7\u7edf\u8ba1\u5b66\u65b9\u6cd5\u4f30\u7b97\u5206\u5b50\u7edd\u5bf9\u6570\u91cf\u3002\u8fd9\u9879\u6280\u672f\u4e3a\u7edd\u5bf9\u5b9a\u91cf\u548c\u7a00\u6709\u7b49\u4f4d\u57fa\u56e0\u68c0\u6d4b\u63d0\u4f9b\u4e86qPCR\u4e4b\u5916\u7684\u66ff\u4ee3\u65b9\u6848\uff0c\u4e0d\u518d\u4f9d\u8d56\u6269\u589e\u5faa\u73af\u6b21\u6570\u6765\u5224\u65ad\u6bcf\u4e2a\u6837\u54c1\u4e2d\u6a21\u677f\u6838\u9178\u7684\u521d\u59cb\u542b\u91cf\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td colspan=\"4\"><p style=\"margin: 4px 0;text-align: center\" align=\"center\"><a title=\"Real-Time vs. Digital PCR vs. Traditional PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/qpcr-education\/qpcr-vs-digital-pcr-vs-traditional-pcr.html?cid=social_btb_genequant\" target=\"_blank\" rel=\"noopener\"><strong>\u6570\u5b57PCR vs. \u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\uff08qPCR\uff09 vs. \u4f20\u7edfPCR\u5bf9\u6bd4\u4e00\u89c8<\/strong><\/a><\/p><\/td><\/tr><tr><td><\/td><td>\n<p style=\"margin: 3px 0;text-align: center\" align=\"center\"><span style=\"font-size: 12px\"><strong>\u6570\u5b57PCR<\/strong><\/span><\/p>\n<\/td><td>\n<p style=\"margin: 3px 0;text-align: center\" align=\"center\"><span style=\"font-size: 12px\"><strong>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\uff08qPCR\uff09<\/strong><\/span><\/p>\n<\/td><td>\n<p style=\"margin: 3px 0;text-align: center\" align=\"center\"><span style=\"font-size: 12px\"><strong>\u4f20\u7edf\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff08PCR\uff09<\/strong><\/span><\/p>\n<\/td><\/tr><tr><td><span style=\"font-size: 12px\"><strong>\u6982\u8ff0<\/strong><\/span><\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u901a\u8fc7\u6d4b\u91cf\u9634\u6027\u5fae\u53cd\u5e94\u6bd4\u4f8b\u6765\u786e\u5b9a\u7edd\u5bf9\u62f7\u8d1d\u6570\u3002<\/p>\n<\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u5728\u6574\u4f53\u53cd\u5e94\u6df7\u5408\u7269\u4e2d\u5b9e\u65f6\u76d1\u6d4bPCR\u6269\u589e\u8fc7\u7a0b\u3002<\/p>\n<\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u5728PCR\u5faa\u73af\u7ed3\u675f\u65f6\u6d4b\u91cf\u7d2f\u79ef\u7684\u4ea7\u7269\u6570\u91cf\u3002<\/p>\n<\/td><\/tr><tr><td><span style=\"font-size: 12px\"><strong>\u5b9a\u91cf\u5417\uff1f<\/strong><\/span><\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u662f\u7684\u2014\u2014\u9634\u6027\u5fae\u53cd\u5e94\u7684\u6bd4\u4f8b\u901a\u8fc7\u6cca\u677e\u7edf\u8ba1\u7b97\u6cd5\u8fdb\u884c\u62df\u5408\u3002<\/p>\n<\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u662f\u7684\u2014\u2014\u6570\u636e\u662f\u5728PCR\u6307\u6570\u589e\u957f\u9636\u6bb5\u6536\u96c6\uff0c\u6b64\u65f6PCR\u4ea7\u7269\u7684\u6570\u91cf\u4e0e\u6a21\u677f\u6838\u9178\u7684\u6570\u91cf\u6210\u6b63\u6bd4\u3002<\/p>\n<\/td><td>\n<p style=\"font-size: 12px;line-height: 16px;margin: 12px 0 12px\">\u4e0d\u662f\u2014\u2014\u4f46\u662f\uff0c\u5c06\u51dd\u80f6\u4e0a\u6269\u589e\u6761\u5e26\u7684\u5f3a\u5ea6\u4e0e\u5df2\u77e5\u6d53\u5ea6\u7684\u6807\u51c6\u8fdb\u884c\u6bd4\u8f83\uff0c\u53ef\u4ee5\u83b7\u5f97\u534a\u5b9a\u91cf\u7ed3\u679c\u3002<\/p>\n<\/td><\/tr><tr><td><span style=\"font-size: 12px\"><strong>\u5e94\u7528\u9886\u57df<\/strong><\/span><\/td><td>\n<ul style=\"font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u75c5\u6bd2\u8f7d\u91cf\u7684\u7edd\u5bf9\u5b9a\u91cf<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u6838\u9178\u6807\u51c6\u54c1\u7684\u7edd\u5bf9\u5b9a\u91cf<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u6587\u5e93\u7684\u7edd\u5bf9\u5b9a\u91cf<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u7a00\u6709\u7b49\u4f4d\u57fa\u56e0\u68c0\u6d4b<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u57fa\u56e0\u8868\u8fbe\u7684\u7edd\u5bf9\u5b9a\u91cf<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5355\u5206\u5b50\u591a\u91cd\u5206\u6790\u591a\u4e2a\u76ee\u6807<\/li>\n<\/ul>\n<\/td><td>\n<ul style=\"list-style-type: none;font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u57fa\u56e0\u8868\u8fbe\u5b9a\u91cf<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5fae\u9635\u5217\u9a8c\u8bc1<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u8d28\u91cf\u63a7\u5236\u4e0e\u68c0\u6d4b\u65b9\u6cd5\u9a8c\u8bc1<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u75c5\u539f\u4f53\u68c0\u6d4b<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">SNP\u57fa\u56e0\u5206\u578b<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u62f7\u8d1d\u6570\u53d8\u5f02\u5206\u6790<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5faeRNA\u5206\u6790<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u75c5\u6bd2\u5b9a\u91cf\u5206\u6790<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">siRNA\/RNAi\u5b9e\u9a8c<\/li>\n<\/ul>\n<\/td><td>\n<ul style=\"list-style-type: none;font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">DNA\u6269\u589e\u7528\u4e8e\uff1a<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u6d4b\u5e8f<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u57fa\u56e0\u5206\u578b<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u514b\u9686<\/li>\n<\/ul>\n<\/td><\/tr><tr><td><span style=\"font-size: 12px\"><strong>\u4f18\u52bf<\/strong><\/span><\/td><td>\n<ul style=\"list-style-type: none;font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u65e0\u9700\u4f9d\u8d56\u53c2\u8003\u7269\u6216\u6807\u51c6\u54c1<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u901a\u8fc7\u589e\u52a0PCR\u91cd\u590d\u6b21\u6570\u53ef\u5b9e\u73b0\u6240\u9700\u7cbe\u5ea6<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5bf9\u67d0\u4e9bPCR\u6291\u5236\u5242\u66f4\u5177\u8010\u53d7\u6027<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u80fd\u591f\u5206\u6790\u590d\u6742\u6df7\u5408\u7269<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5bf9\u5b58\u5728\u7684\u62f7\u8d1d\u6570\u63d0\u4f9b\u7ebf\u6027\u54cd\u5e94\uff0c\u53ef\u68c0\u6d4b\u5fae\u5c0f\u500d\u6570\u53d8\u5316<\/li>\n<\/ul>\n<\/td><td>\n<ul style=\"list-style-type: none;font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u68c0\u6d4b\u7684\u52a8\u6001\u8303\u56f4\u589e\u52a0<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u65e0\u9700PCR\u540e\u5904\u7406<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u53ef\u68c0\u6d4b\u6700\u4f4e\u81f32\u500d\u53d8\u5316<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u5728PCR\u6307\u6570\u589e\u957f\u9636\u6bb5\u6536\u96c6\u6570\u636e<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u62a5\u544a\u8367\u5149\u4fe1\u53f7\u7684\u589e\u52a0\u4e0e\u6269\u589e\u4ea7\u7269\u6570\u91cf\u6210\u6b63\u6bd4<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u88ab\u5207\u5272\u7684\u63a2\u9488\u4e3a\u6269\u589e\u4ea7\u7269\u63d0\u4f9b\u4e86\u6c38\u4e45\u8bb0\u5f55<\/li>\n<\/ul>\n<\/td><td>\n<ul style=\"list-style-type: none;font-size: 12px;line-height: 16px;margin: 12px 0 0 -36px\">\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u8bbe\u8ba1\u7b80\u5355<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u64cd\u4f5c\u5bb9\u6613<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u4f7f\u7528\u66f4\u6613\u83b7\u5f97\u7684\u8bbe\u5907\u548c\u8bd5\u5242<\/li>\n \t<li style=\"list-style-type: none;margin-bottom: 10px\">\u65e0\u9700\u7279\u6b8a\u57f9\u8bad<\/li>\n<\/ul>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6570\u5b57pcr\u7684\u5de5\u4f5c\u539f\u7406\">\u6570\u5b57PCR\u7684\u5de5\u4f5c\u539f\u7406<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-dpcr\u901a\u8fc7\u8ba1\u6570\u5355\u4e2a\u5206\u5b50\u5b9e\u73b0\u7edd\u5bf9\u5b9a\u91cf\">dPCR\u901a\u8fc7\u8ba1\u6570\u5355\u4e2a\u5206\u5b50\u5b9e\u73b0\u7edd\u5bf9\u5b9a\u91cf<\/h3>\n\n\n\n<p>\u4f20\u7edfPCR\u5728\u53cd\u5e94\u5e73\u53f0\u671f\u8fdb\u884c\u6d4b\u91cf\uff0c\u7531\u4e8e\u53cd\u5e94\u52a8\u529b\u5b66\u7684\u53d8\u5316\u5bfc\u81f4\u7ed3\u679c\u4e0d\u7a33\u5b9a\uff1b\u800cqPCR\u5728\u6307\u6570\u671f\u8fdb\u884c\u6d4b\u91cf\uff0c\u80fd\u591f\u66f4\u51c6\u786e\u5730\u5b9a\u91cf\u3002dPCR\u5219\u901a\u8fc7\u8ba1\u6570\u5355\u4e2a\u5206\u5b50\uff0c\u5b9e\u73b0\u7edd\u5bf9\u5b9a\u91cf\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/03\/dPCR-workflow_graphic-1024x305.png\" alt=\"Digital PCR workflow graphic showing flow of Preparation to Digitization to Amplification to Quantification\" class=\"wp-image-16567\" title=\"Digital PCR Workflow - Preparation to Digitization to Amplification to Quantification\" \/><\/figure>\n<\/div>\n\n\n<h4 class=\"wp-block-heading\" id=\"h-dpcr\u4e0e\u5355\u4e2a\u5fae\u53cd\u5e94\u7684\u91cd\u8981\u6027\">dPCR\u4e0e\u5355\u4e2a\u5fae\u53cd\u5e94\u7684\u91cd\u8981\u6027<\/h4>\n\n\n\n<p>dPCR\u901a\u8fc7\u5c06DNA\u3001cDNA\u6216RNA\u6837\u672c\u5206\u6210\u8bb8\u591a\u4e2a\u72ec\u7acb\u7684\u5fae\u53cd\u5e94\u6765\u5de5\u4f5c\uff1b\u6709\u4e9b\u5fae\u53cd\u5e94\u4e2d\u542b\u6709\u4e00\u4e2a\u6216\u591a\u4e2a\u5206\u5b50\uff0c\u800c\u6709\u4e9b\u5219\u6ca1\u6709\u3002\u6bcf\u4e2a\u5fae\u53cd\u5e94\u5206\u522b\u8fdb\u884cPCR\u6269\u589e\u548c\u5206\u6790\u3002\u542b\u6709\u6269\u589e\u4ea7\u7269\u548c\u4e0d\u542b\u6269\u589e\u4ea7\u7269\u7684\u5fae\u53cd\u5e94\u4f1a\u88ab\u5206\u522b\u8ba1\u6570\u3002\u542b\u6709\u6269\u589e\u4ea7\u7269\u7684\u88ab\u5224\u5b9a\u4e3a\u9633\u6027\uff1b\u4e0d\u542b\u6269\u589e\u4ea7\u7269\u7684\u88ab\u5224\u5b9a\u4e3a\u9634\u6027\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5fae\u6d41\u63a7\u677f\u53ef\u5e76\u884c\u8fd0\u884c\u6570\u5343\u4e2a\u5fae\u53cd\u5e94\">\u5fae\u6d41\u63a7\u677f\u53ef\u5e76\u884c\u8fd0\u884c\u6570\u5343\u4e2a\u5fae\u53cd\u5e94<\/h4>\n\n\n\n<p>\u5fae\u6d41\u63a7\u677f\u63d0\u4f9b\u4e86\u4e00\u79cd\u65b9\u4fbf\u4e14\u76f4\u63a5\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u5e76\u884c\u8fd0\u884c\u6570\u5343\u4e2a\u5fae\u53cd\u5e94\u3002\u677f\u4e0a\u7684\u6bcf\u4e00\u4e2a\u4e0a\u6e38\u5b54\u90fd\u88ab\u52a0\u8f7d\u4e86\u6837\u672c\u3001\u4e3b\u6df7\u5408\u6db2\u548c\u8bd5\u5242\u7684\u6df7\u5408\u7269\uff0c\u5728\u5206\u914d\u5230\u5404\u4e2a\u5fae\u53cd\u5e94\u540e\uff0c\u5206\u522b\u5206\u6790\u4ee5\u68c0\u6d4b\u7ec8\u70b9\u4fe1\u53f7\u7684\u5b58\u5728\u4e0e\u5426\u3002<\/p>\n\n\n\n<p>\u5728PCR\u5206\u6790\u4e4b\u540e\uff0c\u9634\u6027\u5fae\u53cd\u5e94\u7684\u6bd4\u4f8b\u7528\u4e8e\u751f\u6210\u6837\u54c1\u4e2d\u76ee\u6807\u5206\u5b50\u7684\u7edd\u5bf9\u6570\u91cf\uff0c\u65e0\u9700\u6807\u51c6\u54c1\u6216\u5185\u6e90\u6027\u5bf9\u7167\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u6821\u6b63\u56e0\u5b50\u7528\u4e8e\u786e\u5b9a\u6a21\u677f\u7684\u7edd\u5bf9\u6570\u91cf\">\u6821\u6b63\u56e0\u5b50\u7528\u4e8e\u786e\u5b9a\u6a21\u677f\u7684\u7edd\u5bf9\u6570\u91cf<\/h4>\n\n\n\n<h5 class=\"wp-block-heading\" id=\"h-\u6cca\u677e\u6a21\u578b\">\u6cca\u677e\u6a21\u578b<\/h5>\n\n\n\n<p>\u7531\u4e8e\u968f\u673a\u5206\u914d\uff0c\u65e0\u6cd5\u4fdd\u8bc1\u6bcf\u4e2a\u9633\u6027\u53cd\u5e94\u53ea\u63a5\u6536\u5230\u4e00\u4e2a\u5206\u5b50\u3002\u4e3a\u4e86\u6821\u6b63\u53ef\u80fd\u63a5\u6536\u5230\u591a\u4e2a\u76ee\u6807\u5e8f\u5217\u5206\u5b50\u7684\u5fae\u53cd\u5e94\uff0c\u9700\u8981\u4f7f\u7528\u6cca\u677e\u6a21\u578b\u5e94\u7528\u4fee\u6b63\u56e0\u5b50\u6765\u786e\u5b9a\u7edd\u5bf9\u6a21\u677f\u6570\u91cf\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/12\/image2.jpg\" alt=\"Scientific graphic - the Poisson Model - bar graph of data\" class=\"wp-image-7001\" title=\"dPCR and the Poisson Model\" \/><\/figure>\n<\/div>\n\n\n<p>\n\n\u5728\u4ee5\u4e0b\u60c5\u51b5\u4e0b\uff1a<a title=\"QuantStudio Absolute Q Digital PCR System\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems\/models\/quantstudio-absolute-q.html\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122 \u6570\u5b57PCR\u7cfb\u7edf<\/a>\uff0c\u6bcf\u4e2a<a title=\"Microfluidic Array Plate Technology - Powerfully Simple Digital PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/microfluidic-array-plate-technology.html\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 QuantStudio MAP16 dPCR \u53cd\u5e94\u677f<\/a>\u5305\u542b20,480\u4e2a\u56fa\u5b9a\u5fae\u8154\u3002\u5b8c\u6210dPCR\u53cd\u5e94\u540e\uff0c<a title=\"QuantStudio Absolute Q Digital PCR System Software\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/quantstudio-absolute-q-software.html\" target=\"_blank\" rel=\"noopener\">QuantStudio Absolute Q \u6570\u5b57PCR\u5206\u6790\u8f6f\u4ef6<\/a>\u4f1a\u8bbe\u5b9a\u9608\u503c\u5e76\u8fdb\u884c\u8ba1\u7b97\u3002\n\n<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/12\/image3.png\" alt=\"Scientific graphic of Poisson Model analysis of expected distribution vs observed\" class=\"wp-image-7002\" title=\"Poisson Model Analysis\" \/><\/figure>\n<\/div>\n\n\n<h5 class=\"wp-block-heading\" id=\"h-\u4f7f\u7528\u6cca\u677e\u6a21\u578b\">\u4f7f\u7528\u6cca\u677e\u6a21\u578b<\/h5>\n\n\n\n<p>\n\u8981\u5e94\u7528\u6cca\u677e\u6a21\u578b\uff0c\u5fc5\u987b\u81f3\u5c11\u6709\u4e00\u4e2a\u9634\u6027\u53cd\u5e94\u2014\u2014\u5373\u6ca1\u6709\u5206\u5b50\u7684\u53cd\u5e94\u3002\u6cca\u677e\u6a21\u578b\u63cf\u8ff0\u4e86\u4e00\u4e2a\u53cd\u5e94\u83b7\u5f97\u96f6\u3001\u4e00\u4e2a\u3001\u4e24\u4e2a\u6216\u4e09\u4e2a\u62f7\u8d1d\u7684\u6982\u7387\uff08\u89c1\u4e0a\u65b9\u67f1\u72b6\u56fe\uff09\u3002\u8be5\u6a21\u578b\u5bf9\u542b\u6709\u591a\u4e2a\u5206\u5b50\u7684\u53cd\u5e94\u8fdb\u884c\u6821\u6b63\uff0c\u5e76\u63d0\u4f9b\u7ed3\u679c\u6b63\u786e\u7684\u6982\u7387\u3002\u56e0\u6b64\uff0c\u6837\u672c\u6570\u5b57\u5316\u7ed3\u5408\u6cca\u677e\u7edf\u8ba1\u6570\u636e\u5206\u6790\uff0c\u6bd4\u4f20\u7edf\u7684PCR\u548cqPCR\u65b9\u6cd5\u5177\u6709\u66f4\u9ad8\u7684\u7cbe\u786e\u5ea6\u3002<br><br>\n\u5728\u4f7f\u7528\u6cca\u677e\u6a21\u578b\u65f6\uff0c\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u9634\u6027\u53cd\u5e94\u6700\u4e3a\u91cd\u8981\uff08\u89c1\u4e0a\u65b996\u5b54\u677f\u7ed3\u679c\uff09\u3002\u5b83\u4eec\u4e0d\u4ec5\u5e2e\u52a9\u786e\u5b9a\u539f\u59cb\u6837\u54c1\u4e2dDNA\u5206\u5b50\u7684\u6570\u91cf\uff0c\u8fd8\u80fd\u5efa\u7acbPCR\u5206\u6790\u540e\u9633\u6027\u4e0e\u9634\u6027\u53cd\u5e94\u7684\u6bd4\u4f8b\u3002<br><br>\n\u4f8b\u5982\uff0c\u6570\u5b57PCR\u7279\u522b\u9002\u7528\u4e8e\u9700\u8981\u68c0\u6d4b\u5c11\u91cf\u8f93\u5165\u6838\u9178\u6216\u5bf9\u6837\u54c1\u4e2d\u76ee\u6807\u6570\u91cf\u8fdb\u884c\u66f4\u7cbe\u7ec6\u5206\u8fa8\u7684\u5e94\u7528\uff0c\u4f8b\u5982\u764c\u75c7\u7814\u7a76\u4e2d\uff0c\u5728\u6db2\u4f53\u6d3b\u68c0\u6837\u54c1\u4e2d\u68c0\u6d4b\u6781\u4f4e\u542b\u91cf\u7684\u764c\u75c7\u7a81\u53d8\u7a00\u6709\u5e8f\u5217\u3002<br><br>\n\u76f8\u5173\u5185\u5bb9: <a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/mutation-detection.html\" target=\"_blank\" rel=\"noopener\">Mutation Detection Using Digital PCR<\/a><\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/12\/image4-1024x349.png\" alt=\"Scientific graphic - precision that one would achieve using either 20K (green), 12K (blue) or 3K (red) microreactions per sample.\" class=\"wp-image-7003\" title=\"dPCR is well suited for applications that require detection of small amounts of input nucleic acid\" \/><\/figure>\n<\/div>\n\n\n<p><span style=\"font-size: 8.0pt\"><strong>\u6807\u9898\uff1a<\/strong>\u5bf9\u4e8e\u4efb\u4f55\u6570\u5b57PCR\u53cd\u5e94\uff0c\u5f53\u6bcf\u4e2a\u53cd\u5e94\u542b\u67091.6\u4e2a\u62f7\u8d1d\u65f6\u53ef\u83b7\u5f97\u6700\u5927\u7cbe\u786e\u5ea6\u3002\u56fe\u8868\u663e\u793a\u4e86\u6bcf\u4e2a\u6837\u54c1\u91c7\u752820K\uff08\u7eff\u8272\uff09\u300112K\uff08\u84dd\u8272\uff09\u62163K\uff08\u7ea2\u8272\uff09\u5fae\u53cd\u5e94\u6240\u80fd\u8fbe\u5230\u7684\u7cbe\u786e\u5ea6\u3002X\u8f74\u4ee3\u8868\u9634\u6027\u53cd\u5e94\uff0820.32%\uff09\uff0cY\u8f74\u4ee3\u8868\u7cbe\u786e\u5ea6\uff0895%\u7f6e\u4fe1\u533a\u95f4\uff09\u3002\u6700\u5927\u7cbe\u786e\u5ea6\u5bf9\u5e94\u4e8e20%\u9634\u6027\u548c80%\u9633\u6027\u53cd\u5e94\u3002\u4ece\u7eff\u8272\u7ebf\u6765\u770b\uff0c20K\u5fae\u53cd\u5e94\/\u6837\u54c1\u65f6\uff0c\u7cbe\u786e\u5ea6\u5728\u7ea65%\u523090%\u7684\u9634\u6027\u53cd\u5e94\u4e4b\u95f4\u4fdd\u6301\u4e00\u81f4\u3002\u4e3a\u4e86\u589e\u52a0\u6bcf\u4e2a\u6837\u54c1\u7684\u5fae\u53cd\u5e94\u6570\uff0c\u53ef\u4ee5\u901a\u8fc7\u6570\u5b57\u6c47\u805a\u63d0\u5347\u7cbe\u786e\u5ea6\u3002\u8fd9\u662f\u8c03\u6574\u6837\u54c1\u6d53\u5ea6\u65f6\u8981\u8ffd\u6c42\u7684\u76ee\u6807\uff0c\u4f8b\u5982\uff0c\u6bcf\u5b54\u4e00\u4e2a\u62f7\u8d1d\u2014\u201415\u5fae\u5347\u5185\u8fdb\u884c20,000\u6b21\u53cd\u5e94\u3002\u5728\u4e24\u7aef\u6781\u503c\u60c5\u51b5\u4e0b\uff0c\u7cbe\u786e\u5ea6\u4f1a\u6025\u5267\u4e0b\u964d\u3002<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u6570\u5b57pcr\u5b9e\u73b0\u9ad8\u6c34\u5e73\u51c6\u786e\u6027\u548c\u91cd\u590d\u6027\">\u6570\u5b57PCR\u5b9e\u73b0\u9ad8\u6c34\u5e73\u51c6\u786e\u6027\u548c\u91cd\u590d\u6027<\/h3>\n\n\n\n<p>\u5728\u8ba8\u8bba\u5e94\u7528\u4e4b\u524d\uff0c\u8fd9\u91cc\u6709\u4e00\u7ec4\u6570\u636e\u5c55\u793a\u4e86\u6570\u5b57PCR\u7684\u91cd\u590d\u6027\u3002<\/p>\n\n\n\n<p>\u4e0b\u56fe\u5c55\u793a\u4e86dPCR\u5728\u7edd\u5bf9\u5b9a\u91cf\u65b9\u9762\u7684\u91cd\u590d\u6027\u3002\u8be5\u56fe\u663e\u793a\u4e86\u4e00\u9879\u7edd\u5bf9\u5b9a\u91cf\u5b9e\u9a8c\u572824\u5757\u677f\u30013\u4f4d\u4e0d\u540c\u7528\u6237\u4e4b\u95f4\u8fd0\u884c\u7684\u7ed3\u679c\u3002\u5982\u56fe\u6240\u793a\uff0c\u8fd9\u4e09\u4f4d\u4e0d\u540c\u7528\u6237\u57fa\u672c\u4e0a\u90fd\u83b7\u5f97\u4e86\u76f8\u540c\u7684\u7ed3\u679c\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/03\/dPCR-consistency_plates_users-1024x739.png\" alt=\"Scientific graphic showing results of an absolute quantification experiment run on 24 plates across 3 different users\" class=\"wp-image-16568\" title=\"Reproducibility of dPCR in Absolute Quantification\" \/><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u6570\u5b57pcr\u9002\u7528\u4e8e\u591a\u79cd\u5e94\u7528\">\u6570\u5b57PCR\u9002\u7528\u4e8e\u591a\u79cd\u5e94\u7528<\/h3>\n\n\n\n<p>dPCR\u975e\u5e38\u9002\u5408\u8fdb\u884c\u7a00\u6709\u7b49\u4f4d\u57fa\u56e0\u68c0\u6d4b\u3001\u62f7\u8d1d\u6570\u53d8\u5f02\u6d4b\u91cf\u3001\u75c5\u6bd2\u6ef4\u5ea6\u6d4b\u91cf\u3001\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u6587\u5e93\u5b9a\u91cf\uff0c\u4ee5\u53ca\u4ece\u73af\u5883\u6837\u672c\uff08\u5982\u5e9f\u6c34\uff09\u4e2d\u68c0\u6d4b\u7a00\u6709\u76ee\u6807\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u7edd\u5bf9\u5b9a\u91cf\u7684\u5177\u4f53\u5e94\u7528\u5305\u62ec\">\u7edd\u5bf9\u5b9a\u91cf\u7684\u5177\u4f53\u5e94\u7528\u5305\u62ec\uff1a<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a title=\"Copy Number Variation Analysis Using TaqMan Assays\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/cnv-analysis-using-taqman.html\" target=\"_blank\" rel=\"noopener\">\u62f7\u8d1d\u6570\u53d8\u5f02\uff08CNV\uff09<\/a> \u2013 \u4ee5\u9ad8\u5ea6\u7cbe\u786e\u6027\u68c0\u6d4b\u548c\u5b9a\u91cf\u5c0f\u6bd4\u4f8b\u7684\u62f7\u8d1d\u6570\u5dee\u5f02<\/li>\n\n\n\n<li><a title=\"Download Digital PCR Tech Notes\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/digital-pcr-tech-notes.html\" target=\"_blank\" rel=\"noopener\">\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08NGS\uff09\u6587\u5e93\u5b9a\u91cf<\/a> \u2013 \u5bf9NGS\u6587\u5e93\u8fdb\u884c\u7edd\u5bf9\u5b9a\u91cf\u5e76\u9a8c\u8bc1\u6d4b\u5e8f\u7ed3\u679c\uff0c\u65e0\u9700\u53c2\u8003\u6807\u51c6\u54c1<\/li>\n\n\n\n<li><a title=\"Mutation Detection Using Digital PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/mutation-detection.html\" target=\"_blank\" rel=\"noopener\">\u7a00\u6709\u76ee\u6807\u5b9a\u91cf<\/a> \u2013 \u68c0\u6d4b\u548c\u5b9a\u91cf\u764c\u75c7\u7814\u7a76\u6837\u672c\u4e2d\u4f4e\u6d41\u884c\u7387\u76ee\u6807\u7684\u7a00\u6709\u7a81\u53d8<\/li>\n\n\n\n<li><a title=\"Cell and Gene Therapy Development Using Digital PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/cell-gene-therapy-manufacturing.html\" target=\"_blank\" rel=\"noopener\">\u75c5\u6bd2\u6ef4\u5ea6\u5b9a\u91cf<\/a> \u2013 \u5bf9\u7ec6\u80de\u548c\u57fa\u56e0\u6cbb\u7597\u7814\u7a76\u4e2d\u5e38\u7528\u7684\u75c5\u6bd2\u8f7d\u4f53\u9aa8\u67b6\uff0c\u5982\u817a\u76f8\u5173\u75c5\u6bd2\uff08AAV\uff09\u548c\u5de8\u7ec6\u80de\u75c5\u6bd2\uff08CMV\uff09\uff0c\u8fdb\u884c\u7edd\u5bf9\u5b9a\u91cf<\/li>\n\n\n\n<li><a title=\"Wastewater Surveillance using Digital PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr\/wastewater-surveillance.html\" target=\"_blank\" rel=\"noopener\">\u73af\u5883\u75c5\u539f\u4f53\u68c0\u6d4b<\/a> \u2013 \u4ece\u73af\u5883\u6837\u672c\u5982\u5e9f\u6c34\u6216\u6c61\u6c34\u6837\u672c\u4e2d\u68c0\u6d4b\u81f4\u75c5\u75c5\u6bd2\u548c\u7ec6\u83cc<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-dpcr\u4f18\u52bf\u603b\u7ed3\">dPCR\u4f18\u52bf\u603b\u7ed3<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-dpcr\u63d0\u4f9b\u7edd\u5bf9\u5b9a\u91cf\u673a\u5236\">dPCR\u63d0\u4f9b\u7edd\u5bf9\u5b9a\u91cf\u673a\u5236<\/h4>\n\n\n\n<p>\u9996\u5148\uff0cdPCR\u63d0\u4f9b\u4e86\u4e00\u79cd\u7edd\u5bf9\u5b9a\u91cf\u7684\u673a\u5236\uff0c\u8ba9\u60a8\u65e0\u9700\u6807\u51c6\u66f2\u7ebf\u5373\u53ef\u786e\u5b9a\u6837\u54c1\u4e2d\u7684\u5206\u5b50\u6570\u91cf\u3002\u8fd9\u5bf9\u4e8e\u53c2\u8003\u5b9e\u9a8c\u5ba4\u548c\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u5c24\u5176\u6709\u76ca\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-dpcr\u53ef\u8bc6\u522b\u7a00\u6709\u5206\u5b50\">dPCR\u53ef\u8bc6\u522b\u7a00\u6709\u5206\u5b50<\/h4>\n\n\n\n<p>\u5176\u6b21\uff0cdPCR\u80fd\u591f\u5728\u5927\u91cf\u6b63\u5e38\u7b49\u4f4d\u57fa\u56e0\u4e2d\u8bc6\u522b\u7a00\u6709\u5206\u5b50\uff0c\u4f8b\u5982\u5728\u5faa\u73af\u6e38\u79bbDNA\u4e2d\u68c0\u6d4b\u81f4\u764c\u7a81\u53d8\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-dpcr\u5bf9\u6291\u5236\u5242\u654f\u611f\u6027\u8f83\u4f4e\">dPCR\u5bf9\u6291\u5236\u5242\u654f\u611f\u6027\u8f83\u4f4e<\/h4>\n\n\n\n<p>\u6700\u540e\uff0c\u7531\u4e8edPCR\u662f\u7ec8\u70b9PCR\u53cd\u5e94\uff0c\u5b83\u5bf9\u6291\u5236\u5242\u7684\u654f\u611f\u6027\u6bd4qPCR\u53cd\u5e94\u4f4e\u3002\u5728qPCR\u53cd\u5e94\u4e2d\uff0c\u50cfSDS\u6216\u809d\u7d20\u8fd9\u6837\u7684\u6291\u5236\u5242\u53ef\u80fd\u4f1a\u5f71\u54cdPCR\u6548\u7387\u3002\u8fd9\u4e00\u7279\u6027\u5bf9\u4e8e\u5904\u7406\u73af\u5883\u6837\u672c\u6216\u73cd\u8d35\u764c\u75c7\u6837\u672c\u7684\u7814\u7a76\u5b9e\u9a8c\u5ba4\u6765\u8bf4\u975e\u5e38\u91cd\u8981\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u6570\u5b57pcr\u7684\u4fe1\u606f\">\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u6570\u5b57PCR\u7684\u4fe1\u606f<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a title=\"Download Digital PCR Technical Literature\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/digital-pcr-tech-notes.html\" target=\"_blank\" rel=\"noopener\">\u4e0b\u8f7d\u6570\u5b57PCR\u6280\u672f\u6587\u732e<\/a><\/li>\n\n\n\n<li><a title=\"Real-Time vs. Digital PCR vs. Traditional PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/qpcr-education\/qpcr-vs-digital-pcr-vs-traditional-pcr.html\" target=\"_blank\" rel=\"noopener\">\u6bd4\u8f83\u4e0e\u5bf9\u6bd4\u6570\u5b57PCR vs. qPCR vs. \u4f20\u7edfPCR<\/a> \u2013 \u4e09\u4ee3PCR\u6280\u672f\u3002<\/li>\n\n\n\n<li><a title=\"QuantStudio Absolute Q Digital PCR System\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems\/models\/quantstudio-absolute-q.html\" target=\"_blank\" rel=\"noopener\">\u83b7\u53d6\u4fe1\u606f\u5173\u4e8eQuantStudio Absolute Q\u6570\u5b57PCR\u7cfb\u7edf<\/a> \u2013 \u4e86\u89e3\u8fd9\u4e00\u529f\u80fd\u5f3a\u5927\u4e14\u7b80\u5355\u7684\u6570\u5b57PCR\u7cfb\u7edf\u3002\u8be5\u5355\u4e00\u4eea\u5668\u7cfb\u7edf\u62e5\u6709\u7c7b\u4f3cqPCR\u7684\u7b80\u4fbf\u5de5\u4f5c\u6d41\u7a0b\uff0c90\u5206\u949f\u5feb\u901f\u51fa\u7ed3\u679c\uff0c\u5e76\u4e14\u65e0\u9700\u53c2\u8003\u66f2\u7ebf\u5373\u53ef\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\uff0c\u5728\u6ca1\u6709\u6807\u51c6\u66f2\u7ebf\u7684\u60c5\u51b5\u4e0b\u4ee5\u62f7\u8d1d\u6570\/\u03bcL\u63d0\u4f9b\u6d53\u5ea6\u6d4b\u91cf\u3002<\/li>\n\n\n\n<li><a title=\"Video - Next-generation digital PCR workflow\" href=\"https:\/\/www.youtube.com\/watch?v=nh9HtzXLYl0\" target=\"_blank\" rel=\"noopener\">\u89c2\u770b\u8fd9\u4e2a\u7b80\u77ed\u89c6\u9891\u5173\u4e8e\u4e0b\u4e00\u4ee3\u6570\u5b57PCR\u5de5\u4f5c\u6d41\u7a0b<\/a>\u4eb2\u81ea\u4f53\u9a8c\u4f7f\u7528 QuantStudio Absolute Q \u6570\u5b57 PCR \u7cfb\u7edf\u8fdb\u884c\u6570\u5b57 PCR \u7684\u5feb\u901f\u4e0e\u7b80\u4fbf\u3002<\/li>\n\n\n\n<li><a title=\"Digital PCR - Powerfully Simple dPCR for Absolute Quantification\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/digital-pcr.html\" target=\"_blank\" rel=\"noopener\">\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u7528\u4e8e\u7edd\u5bf9\u5b9a\u91cf\u7684\u5f3a\u5927\u800c\u7b80\u5355\u7684\u6570\u5b57 PCR<\/a> \u2013 \u63d0\u4f9b\u6709\u5173\u6570\u5b57 PCR \u53ca\u76f8\u5173\u5e94\u7528\u7684\u5b8c\u6574\u4fe1\u606f\uff0c\u5305\u62ec\u65b0\u7684\u548c\u76f8\u5173\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/digital-pcr-tech-notes.html\">\u6280\u672f\u548c\u5e94\u7528\u8bf4\u660e<\/a>\u3001\u89c6\u9891\u4ee5\u53ca\u8d44\u6e90\u3002<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" 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