{"id":528,"date":"2026-02-26T06:23:03","date_gmt":"2026-02-26T06:23:03","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=528"},"modified":"2026-03-09T05:56:16","modified_gmt":"2026-03-09T05:56:16","slug":"how-taqman-assays-work","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/how-taqman-assays-work\/","title":{"rendered":"TaqMan\u68c0\u6d4b\u65b9\u6cd5\u7684\u5de5\u4f5c\u539f\u7406"},"content":{"rendered":"\n
\"qPCR<\/figure>\n\n\n\n

\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff08PCR\uff09\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u6700\u5f3a\u5927\u7684\u6280\u672f\u4e4b\u4e00\u3002\u5229\u7528PCR\uff0c\u53ef\u4ee5\u5c06DNA\u6216\u4e92\u8865DNA\uff08cDNA\uff09\u6a21\u677f\u4e2d\u7684\u7279\u5b9a\u5e8f\u5217\u8fdb\u884c\u6210\u5343\u4e0a\u4e07\u751a\u81f3\u767e\u4e07\u500d\u7684\u590d\u5236\u6216\u201c\u6269\u589e\u201d\u3002\u5728\u4f20\u7edf\u7684\u7ec8\u70b9PCR\u4e2d\uff0c\u6269\u589e\u5e8f\u5217\u7684\u68c0\u6d4b\u548c\u5b9a\u91cf\u662f\u5728\u6700\u540e\u4e00\u4e2aPCR\u5faa\u73af\u7ed3\u675f\u540e\u8fdb\u884c\u7684\uff0c\u5e76\u6d89\u53ca\u51dd\u80f6\u7535\u6cf3\u548c\u56fe\u50cf\u5206\u6790\u7b49\u540e\u7eed\u5206\u6790\u3002\u7531\u4e8e\u53cd\u5e94\u662f\u5728\u5b8c\u6210\u540e\u624d\u88ab\u68c0\u6d4b\uff0c\u7ec8\u70b9PCR\u7684\u6570\u636e\u662f\u534a\u5b9a\u91cf\u7684\uff0c\u901a\u5e38\u7528\u4e8e\u5224\u65ad\u6269\u589e\u662f\u5426\u6210\u529f\u3002\u5728\u5b9e\u65f6\u6216\u5b9a\u91cfPCR\u4e2d\uff0c\u901a\u5e38\u7b80\u79f0\u4e3a\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6216qPCR\uff0c\u6bcf\u4e2a\u5faa\u73af\u90fd\u4f1a\u6d4b\u91cfPCR\u4ea7\u7269\u3002\u901a\u8fc7\u5728\u53cd\u5e94\u7684\u6307\u6570\u6269\u589e\u9636\u6bb5\u76d1\u6d4b\u53cd\u5e94\u8fc7\u7a0b\uff0c\u7528\u6237\u53ef\u4ee5\u975e\u5e38\u7cbe\u786e\u5730\u786e\u5b9a\u76ee\u6807\u521d\u59cb\u6570\u91cf\u3002\u6709\u591a\u79cd\u53cd\u5e94\u5316\u5b66\u65b9\u6cd5\u53ef\u4ee5\u5b9e\u73b0\u8fd9\u4e00\u8fc7\u7a0b\uff0c\u4f46Applied Biosystems\u2122 TaqMan\u2122 \u68c0\u6d4b\u8bd5\u5242\u76d2\u957f\u671f\u4ee5\u6765\u4e00\u76f4\u662f\u4f7fqPCR\u53d8\u5f97\u7b80\u5355\u3001\u7cbe\u786e\u3001\u7279\u5f02\u4e14\u9ad8\u6548\u7684\u91d1\u6807\u51c6\u3002<\/p>\n\n\n\n

\u90a3\u4e48\u5b83\u4eec\u662f\u5982\u4f55\u5de5\u4f5c\u7684\u5462\uff1f<\/p>\n\n\n\n

\u5f15\u7269\u3001\u63a2\u9488\u4e0e\u8367\u5149\u4fe1\u53f7<\/h2>\n\n\n
\n
\"A<\/figure>\n<\/div>\n\n\n

TaqMan\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u6807\u5fd7\u6027\u7279\u70b9\u662f\u63a2\u9488<\/strong>\u3002\u8fd9\u79cd\u4e0e\u88ab\u6d4bDNA\u6a21\u677f\u5339\u914d\u7684\u5c0f\u7247\u6bb5DNA\u4e0a\u8fde\u63a5\u6709\u4e24\u79cd\u7279\u6b8a\u5206\u5b50\uff1a\u4e00\u79cd\u662f\u8367\u5149\u62a5\u544a\u67d3\u6599\uff08R<\/strong>\uff09\uff0c\u53e6\u4e00\u79cd\u662f\u731d\u706d\u5242\uff08Q<\/strong>\uff09\u3002\u5f53\u8fd9\u4e24\u79cd\u5206\u5b50\u90fd\u8fde\u63a5\u5728\u63a2\u9488\u4e0a\u65f6\uff0c\u67d3\u6599\u7684\u8367\u5149\u4f1a\u88ab\u731d\u706d\u5242\u6291\u5236\u3002\u8fd9\u4e9b\u63a2\u9488\u5728\u6a21\u677fDNA\u53d8\u6027\u4e3a\u5355\u94fe\u540e\u4f46\u5c1a\u672a\u5f00\u59cb\u590d\u5236\u524d\u7ed3\u5408\u5230\u6a21\u677f\u4e0a\uff0c\u4ece\u800c\u786e\u4fdd\u6240\u6709\u6a21\u677f\u590d\u5236\u8fc7\u7a0b\u90fd\u80fd\u4e0e\u63a2\u9488\u53d1\u751f\u4f5c\u7528\u3002<\/p>\n\n\n

\n
\"TaqMan\"<\/figure>\n<\/div>\n\n\n

TaqMan\u63a2\u9488\u7684\u4e00\u5927\u72ec\u7279\u4e4b\u5904\u662f\u5728\u51763\u2019\u7aef\u6dfb\u52a0\u4e86\u6b21\u6c9f\u7ed3\u5408\u4f53\uff08MGB\uff09\u57fa\u56e2\uff0c\u8fd9\u4e00\u4fee\u9970\u80fd\u591f\u63d0\u9ad8\u63a2\u9488\u7684\u7194\u89e3\u6e29\u5ea6\uff08Tm\uff09\uff0c\u5e76\u7a33\u5b9a\u63a2\u9488-\u9776\u6807\u6742\u4ea4\u4f53\u3002\u8fd9\u610f\u5473\u7740TaqMan MGB\u63a2\u9488\u53ef\u4ee5\u6bd4\u4f20\u7edf\u63a2\u9488\u663e\u8457\u66f4\u77ed\uff0c\u4ece\u800c\u63d0\u4f9b\u66f4\u597d\u7684\u5e8f\u5217\u8bc6\u522b\u80fd\u529b\uff0c\u5e76\u5177\u5907\u66f4\u5927\u7684\u7075\u6d3b\u6027\u4ee5\u9002\u914d\u66f4\u591a\u9776\u6807\u3002<\/p>\n\n\n

\n
\"Forward<\/figure>\n<\/div>\n\n\nPrimers<\/strong>\n\n\n\ntaq<\/em>\n\n\n\nTaq<\/em> polymerase <\/strong>\n\n\n\nThermus aquaticus, <\/em>\n\n\n\nTaq<\/em>\n\n\n\n

\u5f15\u7269<\/strong>\u662f\u80fd\u591f\u7ed3\u5408\u5230\u6a21\u677f\u94fe\u4e24\u7aef\uff085’\u7aef\u548c3’\u7aef\uff09\u7684\u77edDNA\u5e8f\u5217\u3002\u8fd9\u4e9b\u5e8f\u5217\u7ecf\u8fc7\u7cbe\u5fc3\u8bbe\u8ba1\uff0c\u4ee5\u5c3d\u53ef\u80fd\u7279\u5f02\u5730\u8bc6\u522b\u6a21\u677f\uff0c\u5e76\u4f5c\u4e3a\u53cd\u5e94\u7684\u951a\u70b9\uff0c\u4f7f\u5f97Taq<\/em>\u805a\u5408\u9176\u80fd\u591f\u5f00\u59cb\u590d\u5236DNA\u6a21\u677f\u3002\u5982\u679c\u6ca1\u6709\u4f18\u826f\u7684\u5f15\u7269\uff0cPCR\u53cd\u5e94\u2014\u2014\u65e0\u8bba\u662f\u5b9a\u91cf\u8fd8\u662f\u5176\u4ed6\u7c7b\u578b\u2014\u2014\u90fd\u53ef\u80fd\u65e0\u6cd5\u6269\u589e\u4efb\u4f55DNA\uff0c\u6216\u8005\u7531\u4e8e\u975e\u7279\u5f02\u6027\u6269\u589e\u800c\u5e72\u6270\u5206\u6790\u3002\u8fd9\u5728\u53e6\u4e00\u79cd\u5e38\u89c1\u7684qPCR\u5316\u5b66\u65b9\u6cd5SYBR Green\u67d3\u6599\u4e2d\u5c24\u4e3a\u503c\u5f97\u5173\u6ce8\uff0c\u56e0\u4e3a\u8be5\u67d3\u6599\u80fd\u4e0e\u4efb\u4f55\u53cc\u94feDNA\u7ed3\u5408\uff0c\u56e0\u6b64\u53ef\u80fd\u5bfc\u81f4\u975e\u76ee\u6807\u7247\u6bb5\u7684\u6269\u589e\u3002<\/p>\n\n\n\n

Taq\u805a\u5408\u9176<\/strong>\u662f\u4e00\u79cd\u7279\u6b8a\u7248\u672c\u7684DNA\u805a\u5408\u9176\uff0c\u6765\u6e90\u4e8e\u55dc\u70ed\u83cc\uff08Thermus aquaticus\uff09\uff0c<\/em>\u5b83\u6bd4\u5927\u591a\u6570\u5176\u4ed6\u751f\u7269\u6765\u6e90\u7684DNA\u805a\u5408\u9176\u80fd\u8010\u53d7\u66f4\u9ad8\u6e29\u5ea6\u3002\u8fd9\u4e2aTaq<\/em>\u805a\u5408\u9176\u8fd8\u6709\u4e00\u4e2a\u989d\u5916\u7684\u4f18\u52bf\uff1a\u5916\u5207\u9176\u6d3b\u6027\u3002\u5f53\u805a\u5408\u9176\u9047\u5230\u5df2\u7ecf\u7ed3\u5408\u5728\u5176\u6b63\u5728\u590d\u5236\u7684\u6a21\u677f\u94fe\u4e0a\u7684\u63a2\u9488\u65f6\uff0c\u5b83\u4f1a\u6d88\u5316\u8be5\u63a2\u9488\uff0c\u5e76\u7ee7\u7eed\u590d\u5236\u5269\u4f59\u7684\u94fe\u3002\u5728\u8fd9\u4e2a\u8fc7\u7a0b\u4e2d\uff0c\u805a\u5408\u9176\u5c06\u8367\u5149\u67d3\u6599\u548c\u731d\u706d\u5242\u5f7c\u6b64\u5206\u79bb\uff0c\u89e3\u9664\u731d\u706d\u5242\u4ecb\u5bfc\u7684\u6291\u5236\uff0c\u4ece\u800c\u4ea7\u751f\u8367\u5149\u4fe1\u53f7\uff0c\u88abqPCR\u4eea\u5668\u68c0\u6d4b\u5230\u3002<\/p>\n\n\n

\n
\"Taq<\/figure>\n<\/div>\n\n\n

\u7531\u4e8e\u8fd9\u79cd\u4fe1\u53f7\u4e0e\u53cd\u5e94\u4e2d\u6a21\u677fDNA\u7684\u6570\u91cf\u5b8c\u5168\u6210\u6b63\u6bd4\uff0c\u56e0\u6b64\u4f7f\u5f97qPCR\u5177\u6709\u5b9a\u91cf\u80fd\u529b\u3002

\n\n\u89c2\u770b\u89c6\u9891\uff1aTaqMan\u5b9e\u65f6PCR\u5206\u6790\u5982\u4f55\u5de5\u4f5c\u2014\u2014Taq Talk\u7b2c4\u96c6<\/a>

\n\nTaqMan\u5206\u6790\u76f8\u6bd4\u8bb8\u591a\u5176\u4ed6qPCR\u5316\u5b66\u65b9\u6cd5\u8fd8\u6709\u4e00\u4e2a\u989d\u5916\u4f18\u52bf\uff0c\u5373\u5b83\u4eec\u517c\u5bb9\u591a\u79cd\u4e0d\u540c\u7684\u8367\u5149\u63a2\u9488\u3002\u8fd9\u610f\u5473\u7740\u53ef\u4ee5\u5728\u540c\u4e00\u6b21qPCR\u53cd\u5e94\u4e2d\u540c\u65f6\u68c0\u6d4b\u591a\u4e2aDNA\u6a21\u677f\uff0c\u56e0\u4e3a\u5b83\u4eec\u7684\u8bfb\u6570\u4e0d\u4f1a\u91cd\u53e0\u3002\u8fd9\u88ab\u79f0\u4e3a\u591a\u91cd\u68c0\u6d4b\uff08multiplexing\uff09\u800c\u8fd9\u79cd\u80fd\u529b\u662fSYBR Green\u53cd\u5e94\u6240\u4e0d\u5177\u5907\u7684\uff0c\u5728SYBR Green\u53cd\u5e94\u4e2d\uff0c\u5355\u4e00\u8367\u5149\u67d3\u6599\u4f1a\u5bf9\u53cd\u5e94\u4e2d\u7684\u4efb\u4f55\u53cc\u94feDNA\uff08\u65e0\u8bba\u662f\u5426\u7279\u5f02\uff09\u6fc0\u6d3b\u3002

\n\n\u89c2\u770b\u89c6\u9891\uff1a\u5355\u91cd\u4e0e\u591a\u91cdqPCR\u5b9e\u9a8c\u6ce8\u610f\u4e8b\u9879\u2014\u2014Taq Talk\u7b2c23\u96c6<\/a><\/p>\n\n\n\n

\u7b80\u5316\u5de5\u4f5c\u6d41\u7a0b<\/h2>\n\n\n\n

\n\u6709\u8fdb\u53d6\u5fc3\u7684\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5229\u7528\u6211\u4eec\u7684\u5b9a\u5236\u68c0\u6d4b\u8bbe\u8ba1\u6d41\u7a0b\uff0c\u6839\u636e\u7279\u5b9a\u5e94\u7528\u91cf\u8eab\u5b9a\u5236\u68c0\u6d4b\u65b9\u6cd5\uff0c\u8fd9\u4e9b\u5e94\u7528\u53ef\u80fd\u8fc7\u4e8e\u7a00\u6709\u6216\u7279\u6b8a\uff0c\u65e0\u6cd5\u4f7f\u7528\u73b0\u6210\u89e3\u51b3\u65b9\u6848\u3002\u4f46\u5bf9\u4e8e\u7814\u7a76\u5e38\u89c1\u57fa\u56e0\u9776\u70b9\u7684\u79d1\u5b66\u5bb6\u6765\u8bf4\uff0c\u9884\u8bbe\u8ba1\u7684TaqMan\u68c0\u6d4b\u5927\u5927\u7b80\u5316\u4e86qPCR\u5de5\u4f5c\u6d41\u7a0b\u3002\u4f7f\u7528\u9884\u8bbe\u8ba1\u68c0\u6d4b\uff0c\u65e0\u9700\u989d\u5916\u8bbe\u8ba1\u3001\u4f18\u5316\u3001\u7194\u89e3\u66f2\u7ebf\u5206\u6790\u6216\u5176\u4ed6\u9a8c\u8bc1\u2014\u2014\u53ea\u9700\u6dfb\u52a0\u6837\u54c1\u548c\u4e3b\u6df7\u5408\u6db2\u5373\u53ef\u3002\u6b64\u5916\uff0c\u6bcf\u4e2a\u9884\u8bbe\u8ba1\u7684TaqMan\u68c0\u6d4b\u90fd\u63d0\u4f9b\u6027\u80fd\u4fdd\u8bc1*\uff0c\u8ba9\u60a8\u7684\u7ed3\u679c\u66f4\u5177\u4fe1\u5fc3\u3002Thermo Fisher \u63d0\u4f9b\u8d85\u8fc72100\u4e07\u79cd\u9884\u8bbe\u8ba1TaqMan\u68c0\u6d4b\uff0c\u6db5\u76d6\u5927\u91cf\u57fa\u56e0\u548c\u7269\u79cd\uff0c\u56e0\u6b64\u6781\u6709\u53ef\u80fd\u5df2\u6709\u9002\u7528\u4e8e\u60a8\u7684\u6848\u4f8b\u7684\u4ea7\u54c1\u3002\u5f3a\u5927\u7684\u751f\u7269\u4fe1\u606f\u5b66\u548c\u8bbe\u8ba1\u6d41\u7a0b\u786e\u4fdd\u9884\u8bbe\u8ba1TaqMan\u68c0\u6d4b\u5177\u6709\u4e00\u6d41\u7684\u7279\u5f02\u6027\u3001\u7075\u654f\u5ea6\u548c\u53ef\u91cd\u590d\u6027\uff0c\u6bcf\u6b21\u90fd\u80fd\u83b7\u5f97\u51fa\u8272\u7ed3\u679c\u3002\n\n\u901a\u8fc7Assay Search Wizard\u8f7b\u677e\u627e\u5230\u9884\u8bbe\u8ba1\u7684TaqMan\u68c0\u6d4b<\/a>\n\n\u5982\u9700\u4e86\u89e3\u66f4\u591a\u5173\u4e8eTaqMan\u68c0\u6d4b\u53ca\u5176\u5728\u5b9e\u9a8c\u5ba4\u4e2d\u7684\u4f5c\u7528\uff0c\u8bf7\u53c2\u9605\u6b64\u5173\u4e8e\u5176\u5de5\u4f5c\u539f\u7406\u7684\u7b80\u8981\u4ecb\u7ecd<\/a>,\u6df1\u5165\u89e3\u6790<\/a>\uff0c\u6216\u5bf9\u5176\u8bbe\u8ba1\u7406\u8bba\u4e0e\u5b9e\u8df5\u7684\u63a2\u8ba8<\/a>.\n<\/p>\n\n\n\n

\u4e86\u89e3\u66f4\u591a\u4fe1\u606f<\/strong><\/h2>\n\n\n\n

\u5b9e\u65f6PCR\u6570\u5b57\u624b\u518c2.0<\/a>\uff1a\u63a2\u7d22\u5b9e\u65f6PCR\u6700\u4f73\u5b9e\u8df5\u3001\u57fa\u672c\u539f\u7406\u53ca\u6545\u969c\u6392\u9664\u6280\u5de7\u3002

\n\n\u8bf7\u89c2\u770b\u5b8c\u6574\u7684 Applied Biosystems Taq Talk \u89c6\u9891\u7cfb\u5217<\/a>\u4f5c\u4e3a\u5b9e\u65f6 PCR \u7684\u8d44\u6df1\u4e13\u5bb6\uff0c\u6211\u4eec\u5728\u8be5\u89c6\u9891\u7cfb\u5217\u4e2d\u89e3\u7b54\u60a8\u6700\u5e38\u89c1\u7684\u5b9e\u65f6 PCR \u95ee\u9898\u548c\u6311\u6218\u3002

\n\n*\u9002\u7528\u6761\u6b3e\u548c\u6761\u4ef6\u3002\u8981\u67e5\u770b\u4fdd\u8bc1\u7684\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u8bbf\u95eethermofisher.com\/taqmanguarantee.<\/a>

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