{"id":533,"date":"2026-02-26T03:07:22","date_gmt":"2026-02-26T03:07:22","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=533"},"modified":"2026-03-09T05:57:29","modified_gmt":"2026-03-09T05:57:29","slug":"ten-tips-for-successful-qpcr","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/ten-tips-for-successful-qpcr\/","title":{"rendered":"\u6210\u529fqPCR\u5b9e\u9a8c\u7684\u5341\u4e2a\u6280\u5de7"},"content":{"rendered":"\n

1. \u9ad8\u8d28\u91cfRNA<\/h3>\n\n\n\n

\u964d\u89e3\u6216\u4e0d\u7eaf\u7684RNA\u4f1a\u9650\u5236\u9006\u8f6c\u5f55\u53cd\u5e94\u7684\u6548\u7387\u5e76\u964d\u4f4e\u4ea7\u91cf\u3002<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

\u964d\u89e3\u6216\u4e0d\u7eaf\u7684RNA\u4f1a\u9650\u5236\u9006\u8f6c\u5f55\u53cd\u5e94\u7684\u6548\u7387\u5e76\u964d\u4f4e\u4ea7\u91cf\u3002RNA\u5e94\u4ece\u65b0\u9c9c\u7ec4\u7ec7\u4e2d\u5236\u5907\uff0c\u6216\u4f7f\u7528\u5982Invitrogen\u2122 RNAlater\u00ae<\/a>\u7a33\u5b9a\u6db2\u5904\u7406\u7684\u7ec4\u7ec7\u4e2d\u5236\u5907\u3002\u56e0\u6b64\uff0c\u90e8\u5206RNA\u964d\u89e3\u662f\u53ef\u4ee5\u5bb9\u5fcd\u7684\u3002\u5982\u679c\u65e0\u6cd5\u4f7f\u7528\u5b8c\u5168\u5b8c\u6574\u7684RNA\uff0c\u8bf7\u8bbe\u8ba1\u5f15\u7269\u4f7f\u5176\u4e0e\u76ee\u6807\u57fa\u56e0\u7684\u5185\u90e8\u533a\u57df\u7ed3\u5408\u3002\u8bf7\u6ce8\u610f\uff0c\u5bf9\u4e8e\u771f\u6b63\u7684\u5b9a\u91cfRT-PCR<\/a>\uff0c\u90e8\u5206\u964d\u89e3\u7684RNA\u53ef\u80fd\u65e0\u6cd5\u51c6\u786e\u53cd\u6620\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\u3002\n<\/p>\n\n\n\n

2. \u826f\u597d\u7684\u5f15\u7269\u548c\u63a2\u9488\u8bbe\u8ba1<\/h3>\n\n\n\n

\n\u4e3a\u4e86\u6700\u6709\u6548\u5730\u8bbe\u8ba1\u5b9e\u65f6PCR\u7528\u7684\u5f15\u7269\u548c\u63a2\u9488\u7ec4\u5408\uff0c\u6211\u4eec\u5f3a\u70c8\u5efa\u8bae\u4f7f\u7528\u5f15\u7269\u8bbe\u8ba1\u8f6f\u4ef6\u3002\u5927\u591a\u6570\u5f15\u7269\u8bbe\u8ba1\u7a0b\u5e8f\u90fd\u5305\u542b\u53ef\u8c03\u53c2\u6570\uff0c\u4ee5\u5b9e\u73b0\u6700\u4f73\u5f15\u7269\u548c\u63a2\u9488\u8bbe\u8ba1\u3002\u8fd9\u4e9b\u53c2\u6570\u8003\u8651\u4e86\u5f15\u7269\/\u63a2\u9488Tm\u3001\u4e92\u8865\u6027\u3001\u4e8c\u7ea7\u7ed3\u6784\uff0c\u4ee5\u53ca\u6269\u589e\u7247\u6bb5\u5927\u5c0f\u7b49\u5176\u4ed6\u91cd\u8981\u56e0\u7d20\u3002\u540c\u65f6\u5efa\u8bae\u9650\u5236\u76f8\u540c\u6838\u82f7\u9178\u8fde\u7eed\u51fa\u73b0\u7684\u6570\u91cf\u3002\u5728\u4e3a\u771f\u6838\u751f\u7269\u76ee\u6807\u8bbe\u8ba1\u6269\u589e\u7247\u6bb5\u65f6\uff0c\u5e94\u9009\u62e9\u8de8\u8d8a\u76ee\u6807mRNA\u81f3\u5c11\u4e00\u4e2a\u5916\u663e\u5b50-\u5916\u663e\u5b50\u8fde\u63a5\u5904\u7684PCR\u5f15\u7269\uff0c\u4ee5\u9632\u6b62\u6765\u81ea\u6c61\u67d3\u57fa\u56e0\u7ec4DNA\u7684\u76ee\u6807\u6269\u589e\u3002

\n\n\u5982\u679c\u4f60\u60f3\u5b8c\u5168\u907f\u514d\u8bbe\u8ba1\u7684\u5de5\u4f5c\uff0c\u53ef\u4ee5\u9009\u62e9\u9884\u5148\u8bbe\u8ba1\u597d\u7684 Applied Biosystems\u2122 TaqMan\u00ae \u68c0\u6d4b\u8bd5\u5242<\/a>\u3002\u6bcf\u4e2a\u68c0\u6d4b\u8bd5\u5242\u90fd\u5305\u542b\u6240\u9700\u7684\u4e00\u5207\u2014\u2014\u76ee\u6807\u5f15\u7269\u548c\u4f18\u5316\u7684\u5e8f\u5217\u7279\u5f02\u6027\u63a2\u9488\u2014\u2014\u65e0\u9700\u989d\u5916\u8bbe\u8ba1\u3001\u4f18\u5316\u6216\u5197\u957f\u7684\u7194\u89e3\u66f2\u7ebf\u5206\u6790\u3002\n<\/p>\n\n\n\n

3. \u4f7f\u7528 Master Mix\uff08\u4e3b\u6df7\u5408\u6db2\uff09<\/h3>\n\n\n\n

\n\u4e3b\u6df7\u5408\u6db2\u5c06\u6240\u6709 qPCR \u8bd5\u5242\u6df7\u5408\u5728\u4e00\u8d77\uff0c\u6709\u52a9\u4e8e\u51cf\u5c11\u6837\u54c1\u95f4\u548c\u5b54\u95f4\u7684\u5dee\u5f02\uff0c\u5e76\u63d0\u9ad8\u91cd\u590d\u6027\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u51cf\u5c11\u5b54\u95f4\u5dee\u5f02\uff0c\u8bf7\u4f7f\u7528\u542b\u6709\u53c2\u8003\u67d3\u6599\uff08\u5982 ROX\uff09\u7684\u4e3b\u6df7\u5408\u6db2\u3002\u8bf7\u53c2\u8003\u8fd9\u4e2a\u5b9e\u7528\u7684\u9009\u62e9\u6307\u5357<\/a>\u6765\u4e3a\u4f60\u7684\u5b9e\u9a8c\u9009\u62e9\u5408\u9002\u7684\u4e3b\u6df7\u5408\u6db2\uff08\u4f60\u751a\u81f3\u53ef\u4ee5\u83b7\u5f97\u4e00\u4e2a\u514d\u8d39\u6837\u54c1<\/a>\uff09\u3002\n<\/p>\n\n\n\n

4. \u907f\u514d\u4ea4\u53c9\u6c61\u67d3<\/h3>\n\n\n\n

\nPCR\u533a\u57df\u5185\u7684\u6240\u6709\u8868\u9762\u90fd\u5e94\u5b9a\u671f\u53bb\u6c61\uff0c\u4ee5\u9632\u6b62\u4ea4\u53c9\u6c61\u67d3\u3002\u5efa\u8bae\u4f7f\u7528DNA\u53bb\u6c61\u6eb6\u6db2\uff0c\u5982Invitrogen\u2122 DNAzap<\/a>\u2122 PCR DNA\u964d\u89e3\u6eb6\u6db2\uff0c\u8be5\u6eb6\u6db2\u80fd\u591f\u7834\u574fDNA\u3002\u5e94\u8fd0\u884c\u4e00\u4e2a\u201c\u65e0\u6a21\u677f\u5bf9\u7167\u201d\uff08NTC\uff09\uff0c\u4ee5\u6392\u9664\u8bd5\u5242\u548c\u8868\u9762\u7684\u4ea4\u53c9\u6c61\u67d3\u3002NTC\u5305\u542b\u6240\u6709RT-PCR\u8bd5\u5242<\/a>\u9664\u4e86\n\u6838\u9178\u6a21\u677f\u3002\u901a\u5e38\uff0cDNA\u6216RNA\u53ea\u9700\u7528\u65e0\u6838\u9178\u9176\u6c34\u66ff\u4ee3\u3002\u5728NTC\u4e2d\u4e0d\u5e94\u5408\u6210\u4efb\u4f55\u4ea7\u7269\uff1b\u5982\u679c\u6269\u589e\u51fa\u4ea7\u7269\uff0c\u5219\u8bf4\u660e\u4e00\u4e2a\u6216\u591a\u4e2aRT-PCR\u8bd5\u5242\u88ab\u6269\u589e\u5b50\u6c61\u67d3\u4e86\u3002

\n\n\u5982\u9700\u4e86\u89e3\u66f4\u591a\u4fe1\u606f\uff0c\u8bf7\u9605\u8bfb\u8fd9\u7bc7\u5173\u4e8e\u5728qPCR\u5b9e\u9a8c\u4e2d\u907f\u514d\u6c61\u67d3\u7684\u826f\u597d\u5b9e\u9a8c\u5ba4\u89c4\u8303\u7684\u6587\u7ae0<\/a>\u3002\n<\/p>\n\n\n\n

5. \u4f7f\u7528\u201c\u65e0\u9006\u8f6c\u5f55\u9176\u201d\u5bf9\u7167\uff08No RT Control\uff09<\/h3>\n\n\n\n

\n\u4e4e\u4e0d\u53ef\u80fd\u5b8c\u5168\u6d88\u9664RNA\u5236\u5907\u4e2d\u7684\u57fa\u56e0\u7ec4DNA\u3002\u56e0\u6b64\uff0c\u5728qPCR\u5b9e\u9a8c\u4e2d\u52a0\u5165\u4e00\u4e2a\u51cf\u53bb\u9006\u8f6c\u5f55\u9176\u5bf9\u7167\uff08\u201c\u65e0\u6269\u589e\u5bf9\u7167\u201d\u6216NAC\uff09\u975e\u5e38\u91cd\u8981\u3002\u901a\u5e38\uff0cNAC\u662f\u4e00\u4e2a\u6a21\u62df\u9006\u8f6c\u5f55\u53cd\u5e94\uff0c\u5305\u542b\u6240\u6709\u8bd5\u5242<\/a>\uff0c\u9664\u4e86\u9006\u8f6c\u5f55\u9176\u3002\u5982\u679c\u5728NAC\u4e2d\u89c2\u5bdf\u5230\u4ea7\u7269\uff0c\u8fd9\u5f88\u53ef\u80fd\u8868\u660e\u6837\u54c1\u4e2d\u5b58\u5728\u6c61\u67d3\u7684DNA\u3002

\n\n\u4e00\u4e2a\u66f4\u7b80\u5355\u7684\u89e3\u51b3\u65b9\u6848\u662f\u4f7f\u7528\u8de8\u8d8a\u5916\u663e\u5b50-\u5916\u663e\u5b50\u8fde\u63a5\u4f4d\u70b9\u7684\u68c0\u6d4b\u65b9\u6cd5\u3002\u901a\u8fc7\u5229\u7528\u8fd9\u79cd\u53ea\u5b58\u5728\u4e8eRNA\uff08\u6216cDNA\uff09\u4e2d\u7684\u72ec\u7279\u5e8f\u5217\uff0c\u5c31\u4e0d\u4f1a\u68c0\u6d4b\u5230\u57fa\u56e0\u7ec4DNA\u3002\u5728\u67e5\u627e\u9884\u8bbe\u8ba1TaqMan\u68c0\u6d4b\u8bd5\u5242<\/a>\u65f6\uff0c\u60a8\u53ef\u4ee5\u7b5b\u9009\u90a3\u4e9b\u4e0d\u4f1a\u68c0\u6d4b\u57fa\u56e0\u7ec4DNA\u7684\u8bd5\u5242\u3002\n<\/p>\n\n\n\n

6. \u4f7f\u7528\u5408\u9002\u7684\u5f52\u4e00\u5316\u5bf9\u7167<\/h3>\n\n\n\n

\n\u901a\u8fc7\u5728\u5b9e\u9a8c\u4e2d\u52a0\u5165\u4e00\u4e2a\u4e0d\u53d8\u7684\u5185\u6e90\u6027\u5bf9\u7167<\/a>\uff0c\u53ef\u4ee5\u63d0\u9ad8\u4efb\u4f55qPCR\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff0c\u4ee5\u6821\u6b63\u6837\u54c1\u95f4\u7684\u5dee\u5f02\u3002\u4e00\u4e2a\u597d\u7684\u5bf9\u7167\u5176\u8868\u8fbe\u6c34\u5e73\u5728\u6240\u5206\u6790\u6837\u54c1\u4e4b\u95f4\u4e0d\u5e94\u6709\u53d8\u5316\u300218S rRNA\u5e38\u88ab\u7528\u4f5c\u5bf9\u7167\uff0c\u56e0\u4e3a\u5176\u8868\u8fbe\u6c34\u5e73\u6bd4\u5176\u4ed6\u4f20\u7edf\u5185\u53c2\u5982\u03b2-\u808c\u52a8\u86cb\u767d\u6216GAPDH\u66f4\u4e3a\u7a33\u5b9a\u3002\u5982\u9700\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u9009\u62e9\u5185\u6e90\u6027\u5bf9\u7167\u7684\u4fe1\u606f\uff0c\u8bf7\u4e0b\u8f7d\u6b64\u4efd\u5e94\u7528\u8bf4\u660e<\/a>\u3002<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

7. \u4f7f\u7528 SYBR\u00ae Green \u8fdb\u884c\u89e3\u79bb\uff08\u7194\u89e3\uff09\u66f2\u7ebf\u5206\u6790<\/h3>\n\n\n\n

\n\u7406\u60f3\u60c5\u51b5\u4e0b\uff0c\u5b9e\u9a8c\u6837\u54c1\u5728\u6269\u589e\u5b50\u7194\u89e3\u6e29\u5ea6\u5904\u5e94\u4ea7\u751f\u4e00\u4e2a\u5c16\u9510\u7684\u5cf0\u503c\uff08\u4e00\u9636\u5bfc\u6570\u56fe\uff09\uff0c\u800c NAC \u548c NTC \u4e0d\u4f1a\u4ea7\u751f\u663e\u8457\u7684\u8367\u5149\u4fe1\u53f7\u3002\u8be5\u7ed3\u679c\u8868\u660e\u4ea7\u7269\u5177\u6709\u7279\u5f02\u6027\uff0c\u5e76\u4e14SYBR Green<\/a>\u8367\u5149\u662f\u76ee\u6807\u4ea7\u7269\u79ef\u7d2f\u7684\u76f4\u63a5\u6d4b\u91cf\u6307\u6807\u3002\u5982\u679c\u89e3\u79bb\u66f2\u7ebf\u663e\u793a\u4e00\u7cfb\u5217\u5cf0\u503c\uff0c\u5219\u8bf4\u660e\u5bf9\u7279\u5f02\u6027\u548c\u975e\u7279\u5f02\u6027\u53cd\u5e94\u4ea7\u7269\u7684\u533a\u5206\u4e0d\u591f\u3002\u4e3a\u4e86\u83b7\u5f97\u6709\u610f\u4e49\u7684\u6570\u636e\uff0c\u9700\u8981\u8fdb\u884c\u4f18\u5316\u3002

\n\n\u4f7f\u7528 TaqMan \u68c0\u6d4b\u8bd5\u5242\u907f\u514d\u4f18\u5316\u548c\u7194\u89e3\u66f2\u7ebf\u5206\u6790\u7684\u5fc5\u8981\u6027\u3002\u9884\u8bbe\u8ba1\u3001\u57fa\u4e8e\u63a2\u9488\u7684 TaqMan \u68c0\u6d4b\u8bd5\u5242<\/a> \u4e0d\u4ec5\u4e3a\u60a8\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u8fd8\u63d0\u4f9b\u4e86\u6027\u80fd\u4fdd\u8bc1<\/a>\u3002\n<\/p>\n\n\n\n

8. \u6b63\u786e\u8bbe\u7f6e\u57fa\u7ebf\u548c\u9608\u503c<\/h3>\n\n\n\n

\n\u4e3a\u4e86\u83b7\u5f97\u51c6\u786e\u7684 Ct \u503c\uff0c\u57fa\u7ebf\u9700\u8981\u6bd4\u6700\u4e30\u5bcc\u6837\u54c1\u7684 Ct \u503c\u63d0\u524d\u4e24\u4e2a\u5faa\u73af\u8bbe\u7f6e\u3002\u8981\u4f7f\u5b9e\u65f6 PCR \u6570\u636e\u6709\u610f\u4e49\uff0c\u9608\u503c\u5e94\u5728\u4ea7\u7269\u5904\u4e8e\u6307\u6570\u589e\u957f\u9636\u6bb5\u65f6\u8bbe\u5b9a\u3002\u65b0\u7684 Applied Biosystems \u4eea\u5668\u548c Thermo Fisher Connect\uff08\u4e91\u7aef\uff09\u90fd\u5177\u5907\u81ea\u52a8\u8bbe\u7f6e\u57fa\u7ebf\u548c\u9608\u503c\u7684\u7b97\u6cd5\u3002\u4e00\u79cd\u540d\u4e3a\u76f8\u5bf9\u9608\u503c\u6216 C \u7684\u65b0\u7b97\u6cd5RT\u53ef\u4ee5\u7528\u4e8e\u786e\u5b9aCq\u3002\u8bf7\u67e5\u9605\u5e94\u7528\u8bf4\u660e\uff0cCRT<\/sub>\uff0c\u4e00\u79cd\u7528\u4e8eqPCR\u6570\u636e\u5206\u6790\u7684\u76f8\u5bf9\u9608\u503c\u65b9\u6cd5<\/a>\u2026<\/a>\uff0c\u4ee5\u83b7\u53d6\u66f4\u591a\u4fe1\u606f\u3002\n\n<\/p>\n\n\n\n

\u5feb\u901f\u56de\u987e\uff1a\u76f8\u5bf9\u9608\u503c\uff08Crt\uff09\u65b9\u6cd5\u3002<\/strong><\/p>\n\n\n\n

\u4e0b\u56fe\u548c\u6b65\u9aa4\u63cf\u8ff0\u4e86\u5982\u4f55\u8ba1\u7b97Crt\u3002\u6269\u589e\u66f2\u7ebf\u4e3a\u84dd\u8272\uff1b\u53cd\u5e94\u6548\u7387\u66f2\u7ebf\u6a21\u578b\u4e3a\u7ea2\u8272\uff1b\u5de6\u4fa7y\u8f74\u5bf9\u5e94\u84dd\u8272\u6269\u589e\u66f2\u7ebf\uff0c\u53f3\u4fa7y\u8f74\u5bf9\u5e94\u7ea2\u8272\u66f2\u7ebf\u3002Crt\u7684\u786e\u5b9a\u5206\u4e3a\u56db\u4e2a\u6b65\u9aa4\uff1a
\u4f7f\u7528\u9884\u8bbe\u7684\u5185\u90e8\u53c2\u8003\u6548\u7387\u6c34\u5e73\uff08\u7c89\u8272\u865a\u7ebf\uff09\u6765\u8bc6\u522b\u53cd\u5e94\u6548\u7387\u66f2\u7ebf\uff08\u6a21\u578b\uff09\u8fbe\u5230\u7279\u5b9a\u6570\u503c\u65f6\u7684\u5c0f\u6570\u5468\u671f\uff08Ce\uff09\u3002<\/p>\n\n\n\n

    \n
  1. \u4f7f\u7528\u9884\u8bbe\u7684\u5185\u90e8\u53c2\u8003\u6548\u7387\u6c34\u5e73\uff08\u7c89\u8272\u865a\u7ebf\uff09\u6765\u786e\u5b9a\u53cd\u5e94\u6548\u7387\u66f2\u7ebf\uff08\u6a21\u578b\uff09\u8fbe\u5230\u7279\u5b9a\u503c\u65f6\u7684\u5206\u6570\u5468\u671f\uff08Ce\uff09\u3002<\/li>\n\n\n\n
  2. \u8367\u5149\u6c34\u5e73\uff08Fe\uff09\u5bf9\u5e94\u4e8e\u6269\u589e\u66f2\u7ebf\u4e0a\u7684\u5206\u6570\u5faa\u73afCe\u3002<\/li>\n\n\n\n
  3. \u76f8\u5bf9\u8367\u5149\u9608\u503c\uff08\u6d45\u84dd\u8272\u865a\u7ebf\uff09\u662f\u6839\u636eFe\u7684\u7279\u5b9a\u767e\u5206\u6bd4\uff08%Fe\uff09\u8ba1\u7b97\u5f97\u51fa\u7684\u66f2\u7ebf\u7279\u5f02\u6027\u9608\u503c\u3002<\/li>\n\n\n\n
  4. Crt\u88ab\u8ba1\u7b97\u4e3a\u6269\u589e\u66f2\u7ebf\u4e0e\u76f8\u5bf9\u8367\u5149\u9608\u503c\u4ea4\u53c9\u65f6\u7684\u5206\u6570\u5faa\u73af\u3002<\/li>\n<\/ol>\n\n\n\n\n\n

    9. \u786e\u8ba4\u53cd\u5e94\u6548\u7387<\/h3>\n\n\n\n

    \u53cd\u5e94\u7684\u6548\u7387\uff08Eff\uff09\u53ef\u901a\u8fc7\u4ee5\u4e0b\u516c\u5f0f\u8ba1\u7b97\uff1a<\/p>\n\n\n\n

    \"\"<\/figure>\n\n\n\n

    PCR\u7684\u6548\u7387\u5e94\u4e3a90\u2013110%\uff083.6 > \u659c\u7387 > 3.1\uff09\u3002\u591a\u79cd\u53d8\u91cf\u4f1a\u5f71\u54cdPCR\u7684\u6548\u7387\uff0c\u5305\u62ec\u6269\u589e\u5b50\u957f\u5ea6\u3001\u4e8c\u7ea7\u7ed3\u6784\u548c\u5f15\u7269\u8bbe\u8ba1\u7b49\u3002\u867d\u7136\u53ef\u4ee5\u83b7\u5f97\u843d\u5728\u8be5\u6548\u7387\u8303\u56f4\u4e4b\u5916\u7684\u6709\u6548\u6570\u636e\uff0c\u4f46\u4ecd\u5efa\u8bae\u8fdb\u4e00\u6b65\u4f18\u5316qPCR\uff0c\u6216\u8bbe\u8ba1\u66ff\u4ee3\u6269\u589e\u5b50\u3002<\/p>\n\n\n\n

    10. \u4f7f\u7528\u9002\u5f53\u8303\u56f4\u7684\u6807\u51c6\u66f2\u7ebf<\/h3>\n\n\n\n

    \u5bf9\u4e8eRNA\u5b9a\u91cf\uff08\u7edd\u5bf9\u6216\u76f8\u5bf9\u5b9a\u91cf\uff09\u7814\u7a76\u4e2d\u7684\u6bcf\u4e2a\u57fa\u56e0\uff0c\u6216\u7528\u4e8e\u6bd4\u8f83\u5b9a\u91cf\uff08delta-delta-Ct\uff09\u4e2d\u9a8c\u8bc1\u53cd\u5e94\u6548\u7387\uff0c\u5e94\u5206\u522b\u5236\u5907\u6807\u51c6\u66f2\u7ebf\u3002\u6807\u51c6\u66f2\u7ebf\u5e94\u8986\u76d6\u76ee\u6807\u9884\u671f\u4e30\u5ea6\u4ee5\u4e0a\u548c\u4ee5\u4e0b\u7684\u8303\u56f4\u3002\u8fd8\u53ef\u4ee5\u5305\u62ec\u5176\u4ed6\u8f93\u5165\u91cf\uff0c\u5982\u9ad8\u4e8e\u548c\u4f4e\u4e8e\u68c0\u6d4b\u9650\u7684\u6700\u5c0f\u548c\u6700\u5927RNA\u7528\u91cf\uff0c\u4ee5\u5e2e\u52a9\u533a\u5206\u7279\u5f02\u6027\u4ea7\u7269\u548c\u975e\u7279\u5f02\u6027\u4ea7\u7269\u3002<\/p>\n\n\n

    \n
    \"\"<\/figure>\n<\/div>\n\n\n

    \u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/em><\/p>\n\n\n\n

    \"contact<\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"

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\u964d\u89e3\u6216\u4e0d\u7eaf\u7684RNA\u4f1a\u9650\u5236\u9006\u8f6c\u5f55\u53cd\u5e94\u7684<\/p>\n","protected":false},"author":1852,"featured_media":534,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[8],"tags":[16,17],"division":[10],"class_list":{"0":"post-533","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-8","8":"tag-qpcr","9":"tag-pcr","10":"division-gsd","11":"entry"},"_selected_authors":[744],"_selected_reviewers":[],"acf":[],"yoast_head":"\n\u6210\u529fqPCR\u5b9e\u9a8c\u7684\u5341\u4e2a\u6280\u5de7 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