{"id":567,"date":"2026-02-27T03:14:29","date_gmt":"2026-02-27T03:14:29","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=567"},"modified":"2026-03-09T05:54:29","modified_gmt":"2026-03-09T05:54:29","slug":"restriction-endonuclease-101-from-basics-to-golden-gate-cloning","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/restriction-endonuclease-101-from-basics-to-golden-gate-cloning\/","title":{"rendered":"\u9650\u5236\u6027\u5185\u5207\u9176\u5165\u95e8\uff1a\u4ece\u57fa\u7840\u77e5\u8bc6\u5230 Golden Gate \u514b\u9686"},"content":{"rendered":"<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/09\/RE-Blog-Image-1024x668.jpg\" alt=\"Restriction Endonuclease 101\" class=\"wp-image-5001\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u51e0\u5341\u5e74\u6765\u4e00\u76f4\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u7684\u57fa\u7840\u5de5\u5177\uff0c\u901a\u5e38\u88ab\u8ba4\u4e3a\u662f\u7b80\u5355\u7684DNA\u5207\u5272\u5de5\u5177\u3002\u7136\u800c\uff0c\u8fd9\u4e9b\u201c\u5206\u5b50\u526a\u5200\u201d\u6bd4\u8868\u9762\u4e0a\u770b\u8d77\u6765\u8981\u590d\u6742\u548c\u6709\u8da3\u5f97\u591a\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-yoast-seo-table-of-contents yoast-table-of-contents\"><h2>\u76ee\u5f55<\/h2><ul><li><a href=\"#h-\u4ec0\u4e48\u662f\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\" data-level=\"2\">\u4ec0\u4e48\u662f\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\uff1f<\/a><\/li><li><a href=\"#h-\u4e3b\u8981\u7c7b\u578b\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\" data-level=\"2\">\u4e3b\u8981\u7c7b\u578b\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176<\/a><\/li><li><a href=\"#h-\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u5207\u5272\u4f4d\u70b9\" data-level=\"2\">\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u5207\u5272\u4f4d\u70b9<\/a><\/li><li><a href=\"#h-\u4f7f\u7528iis\u578b\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176-golden-gate\u514b\u9686\u6280\u672f\" data-level=\"2\">\u4f7f\u7528IIS\u578b\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\uff1aGolden Gate\u514b\u9686\u6280\u672f<\/a><\/li><li><a href=\"#h-\u65e0\u7f1dgolden-gate\u7ec4\u88c5\u6280\u5de7\" data-level=\"2\">\u65e0\u7f1dGolden Gate\u7ec4\u88c5\u6280\u5de7<\/a><\/li><li><a href=\"#h-\u5e94\u7528-\u690d\u7269\u5de5\u7a0b\u4e2d\u7684golden-gate\u514b\u9686\u6280\u672f\" data-level=\"2\">\u5e94\u7528\uff1a\u690d\u7269\u5de5\u7a0b\u4e2d\u7684Golden Gate\u514b\u9686\u6280\u672f<\/a><\/li><li><a href=\"#h-\u5173\u4e8e\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u66f4\u591a\u8d44\u6e90\" data-level=\"2\">\u5173\u4e8e\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u66f4\u591a\u8d44\u6e90<\/a><\/li><li><a href=\"#h-\u53c2\u8003\u6587\u732e\" data-level=\"2\">\u53c2\u8003\u6587\u732e<\/a><\/li><\/ul><\/div>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u63a2\u7d22\u8d5b\u9ed8\u98de\u4e16\u5c14\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662f\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\">\u4ec0\u4e48\u662f\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\uff1f<\/h2>\n\n\n\n<p>\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\uff0c\u4e5f\u88ab\u79f0\u4e3a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific.html\">\u9650\u5236\u9176<\/a>\uff0c\u662f\u4e00\u7c7b\u7531\u7ec6\u83cc\u548c\u4e00\u4e9b\u53e4\u83cc\u4ea7\u751f\u7684\u7279\u6b8a\u86cb\u767d\u8d28\uff0c\u80fd\u591f\u5728\u7279\u5b9a\u5e8f\u5217\uff08\u79f0\u4e3a\u9650\u5236\u4f4d\u70b9\uff09\u4e0a\u5207\u5272\uff08\u901a\u5e38\u662f\u53cc\u94fe\uff09DNA\u3002<\/p>\n\n\n\n<p>\u9650\u5236\u4f4d\u70b9\u901a\u5e38\u662f\u77ed\u7684\u56de\u6587\u5e8f\u5217\uff0c\u957f\u5ea6\u4e3a\u56db\u5230\u516b\u4e2a\u78b1\u57fa\u5bf9\u3002\u5728\u8fd9\u4e9b\u8bc6\u522b\u4f4d\u70b9\u4e0a\u7684\u7532\u57fa\u5316\u53ef\u4ee5\u9632\u6b62\u5bbf\u4e3bDNA\u81ea\u8eab\u88ab\u6d88\u5316\uff0c\u4f7f\u5176\u5bf9\u81ea\u8eab\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u5207\u5272\u5177\u6709\u6297\u6027\u3002<\/p>\n\n\n\n<p>\u7531\u9650\u5236\u9176\u4ea7\u751f\u7684DNA\u7247\u6bb5\u53ef\u4ee5\u5177\u6709\u7c98\u6027\u672b\u7aef\u6216\u5e73\u672b\u7aef\uff0c\u8fd9\u53d6\u51b3\u4e8e\u9176\u5982\u4f55\u5207\u5272DNA\u94fe\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7c98\u6027\u672b\u7aef<\/strong>\u8fd9\u4e9b\u662f\u7531\u9650\u5236\u6027\u9176\u5728DNA\u4e0a\u4ea7\u751f\u9519\u4f4d\u5207\u5272\u65f6\u5f62\u6210\u7684\u5355\u94fe\u7a81\u51fa\u7aef\u3002\u8fd9\u4e9b\u7a81\u51fa\u7aef\u4f4d\u4e8e5\u2032\u62163\u2032\u672b\u7aef\uff0c\u53ef\u4ee5\u4e0e\u5176\u4ed6DNA\u7247\u6bb5\u4e0a\u7684\u4e92\u8865\u5e8f\u5217\u5f62\u6210\u6c22\u952e\u3002\u7531\u4e8e\u8fd9\u79cd\u7279\u6027\uff0c\u7c98\u6027\u672b\u7aef\u5728\u5206\u5b50\u514b\u9686\u4e2d\u5c24\u4e3a\u6709\u7528\uff0c\u56e0\u4e3a\u5b83\u4eec\u901a\u8fc7\u78b1\u57fa\u914d\u5bf9\u4fc3\u8fdbDNA\u7247\u6bb5\u7684\u7cbe\u786e\u9ad8\u6548\u8fde\u63a5\uff0c\u968f\u540e\u518d\u7531DNA\u8fde\u63a5\u9176\u5c01\u95ed\u3002<br><\/li>\n\n\n\n<li><strong>\u5e73\u672b\u7aef<\/strong>\u8fd9\u4e9b\u662f\u5728\u9650\u5236\u6027\u9176\u76f4\u63a5\u5207\u5272\u4e24\u6761DNA\u94fe\u65f6\u4ea7\u751f\u7684\uff0c\u7ed3\u679c\u662f\u6ca1\u6709\u7a81\u51fa\u7aef\u7684\u7247\u6bb5\u3002\u7531\u4e8e\u5e73\u672b\u7aef\u7f3a\u4e4f\u4e92\u8865\u7a81\u51fa\u7aef\uff0c\u5728\u8fde\u63a5\u8fc7\u7a0b\u4e2d\u6548\u7387\u4f4e\u4e8e\u7c98\u6027\u672b\u7aef\u3002\u7136\u800c\uff0c\u5b83\u4eec\u5177\u6709\u901a\u7528\u517c\u5bb9\u6027\u7684\u4f18\u52bf\uff0c\u5373\u4efb\u4f55\u4e24\u4e2a\u5e73\u672b\u7aef\u7247\u6bb5\u90fd\u53ef\u4ee5\u8fde\u63a5\u5728\u4e00\u8d77\uff0c\u800c\u4e0d\u53d7\u5176\u5e8f\u5217\u5f71\u54cd\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u76ee\u524d\u5df2\u77e5\u6709\u8d85\u8fc74,000\u79cd\u9650\u5236\u6027\u9176\uff0c\u9488\u5bf9400\u591a\u4e2a\u8bc6\u522b\u5e8f\u5217\u3002<\/p>\n\n\n\n<p>\u5173\u4e8e\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u53d1\u5c55\u7684\u91cc\u7a0b\u7891\u4e8b\u4ef6\uff0c<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/molecular-cloning\/restriction-enzymes\/restriction-enzyme-basics.html?cid=bid_mol_clo_r01_co_cp1613_pjt12393_col000000_0so_blg_il_awa_kt_s18_RestEnzyBlg1\" target=\"_blank\" rel=\"noreferrer noopener\">\u8bf7\u53c2\u9605\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u57fa\u7840\u77e5\u8bc6<\/a>\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e3b\u8981\u7c7b\u578b\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\">\u4e3b\u8981\u7c7b\u578b\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176<\/h2>\n\n\n\n<p>\u6839\u636e\u7ed3\u6784\u590d\u6742\u5ea6\u3001\u8bc6\u522b\u4f4d\u70b9\u3001\u5207\u5272\u4f4d\u70b9\u548c\u8f85\u56e0\u5b50\u9700\u6c42\uff0c\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u5206\u4e3a\u56db\u5927\u7c7b\u6216\u7c7b\u578b\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\"><table><thead><tr><th>\u9176\u7c7b\u522b<\/th><th>\u7279\u5f81<\/th><\/tr><\/thead><tbody><tr><td><strong>I\u578b<\/strong><\/td><td>\u2013 \u591a\u4e9a\u57fa\u86cb\u767d\uff0c\u5177\u6709\u9650\u5236\u548c\u7532\u57fa\u5316\u53cc\u91cd\u6d3b\u6027<br>\n\u2013 \u9700\u8981SAM\u3001ATP\u548cMg<sup>2+<\/sup>\u9700\u6c42\u6761\u4ef6<br>\n\u2013 \u5207\u5272\u4f4d\u70b9\u8ddd\u79bb\u8bc6\u522b\u4f4d\u70b9\u6709\u53ef\u53d8\u8ddd\u79bb<\/td><\/tr><tr><td><strong>II\u578b<\/strong><\/td><td>\u2013 \u5177\u6709\u7279\u5f02\u6027\u8bc6\u522b\u5e8f\u5217\uff0c\u5207\u5272\u4f4d\u70b9\u5728\u8bc6\u522b\u5e8f\u5217\u5185\u6216\u9644\u8fd1<br>\n\u2013 \u5728\u5207\u5272\u4f4d\u70b9\u4ea7\u751f5\u2032\u78f7\u9178\u548c3\u2032\u7f9f\u57fa\u672b\u7aef<br>\n\u2013 Mg<sup>2+<\/sup>\u5927\u591a\u6570\u7684\u8981\u6c42<\/td><\/tr><tr><td><strong>III\u578b<\/strong><\/td><td>\u2013 \u4e24\u90e8\u5206\u8bc6\u522b\u5e8f\u5217\u5448\u53cd\u5411\u6392\u5217<br>\n\u2013 \u5207\u5272\u4f4d\u70b9\u8ddd\u79bb\u5176\u4e2d\u4e00\u4e2a\u8bc6\u522b\u5e8f\u5217\u6709\u7279\u5b9a\u8ddd\u79bb<br>\n\u2013 \u9700\u8981ATP<\/td><\/tr><tr><td><strong>IV\u578b<\/strong><\/td><td>\u2013 \u53ea\u5207\u5272\u7532\u57fa\u5316DNA<br>\n\u2013 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class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>II\u578b\u9650\u5236\u6027\u9176\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u6700\u5e38\u7528\u7684\uff0c\u56e0\u4e3a\u5b83\u4eec\u80fd\u591f\u5728\u5176\u8bc6\u522b\u5e8f\u5217\u5185\u6216\u9644\u8fd1\u7684\u7279\u5b9a\u4f4d\u70b9\u5207\u5272DNA\uff0c\u800c\u65e0\u9700ATP\u3002<\/p>\n\n\n\n<p>IIS\u578b\u9650\u5236\u6027\u5185\u5207\u9176\u5728\u5176\u4f5c\u7528\u673a\u5236\u4e0a\u5177\u6709\u72ec\u7279\u6027\u3002\u4e0e\u8bc6\u522b\u56de\u6587\u5e8f\u5217\u5e76\u5728\u5176\u8bc6\u522b\u4f4d\u70b9\u5185\u6216\u7d27\u90bb\u5904\u5207\u5272DNA\u7684IIP\u578b\u9176\u4e0d\u540c\uff0cIIS\u578b\u9176\u8bc6\u522b\u975e\u56de\u6587DNA\u5e8f\u5217\uff0c\u5e76\u5728\u8ddd\u79bb\u8be5\u5e8f\u5217\u4e00\u5b9a\u8ddd\u79bb\u7684\u4f4d\u7f6e\u8fdb\u884c\u5207\u5272\u3002\u8fd9\u4e00\u72ec\u7279\u6027\u8d28\u5728Golden Gate\u7ec4\u88c5\u4e2d\u975e\u5e38\u6709\u7528\uff0c\u56e0\u4e3a\u53ef\u4ee5\u751f\u6210\u5e26\u6709\u81ea\u5b9a\u4e49\u957f\u5ea6\u548c\u5e8f\u5217\u7684\u7c98\u6027\u672b\u7aef\u7684DNA\u7247\u6bb5\uff0c\u4ece\u800c\u5b9e\u73b0\u7cbe\u786e\u4e14\u65e0\u7f1d\u5730\u8fde\u63a5\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aae269f74e96&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aae269f74e96\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/09\/RE2-1024x314.png\" alt=\"Type II restriction endonucleases\" class=\"wp-image-5002\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u5207\u5272\u4f4d\u70b9\">\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u5207\u5272\u4f4d\u70b9<\/h2>\n\n\n\n<p><strong>\u540c\u5de5\u9650\u5236\u9176<\/strong>\u662f\u6765\u81ea\u4e0d\u540c\u751f\u7269\u4f46\u5177\u6709\u76f8\u540c\u9650\u5236\u4f4d\u70b9\u5e76\u5728\u76f8\u540c\u4f4d\u7f6e\u5207\u5272DNA\u7684\u9650\u5236\u6027\u5185\u5207\u9176\u3002\u4f8b\u5982\uff0c\u4e00\u5bf9\u9650\u5236\u6027\u5185\u5207\u9176<em>Spe<\/em>I \u548c<em>Bcu<\/em>I \u90fd\u80fd\u8bc6\u522b\u5e8f\u5217 5\u2032-A\u2193CTAGT-3\u2032 \u5e76\u5728\u76f8\u540c\u4f4d\u7f6e\u8fdb\u884c\u5207\u5272\uff0c\u56e0\u6b64\u8fd9\u4e24\u79cd\u9176\u53ef\u4ee5\u4e92\u6362\u4f7f\u7528\u3002<\/p>\n\n\n\n<p><strong>\u65b0\u540c\u5de5\u9650\u5236\u9176<\/strong>\u662f\u540c\u5de5\u9650\u5236\u9176\u7684\u4e00\u7c7b\uff0c\u5b83\u4eec\u80fd\u591f\u8bc6\u522b\u76f8\u540c\u7684\u5e8f\u5217\uff0c\u4f46\u4f1a\u5728\u4e0d\u540c\u7684\u4f4d\u7f6e\u5207\u5272DNA\uff0c\u4ece\u800c\u4ea7\u751f\u591a\u6837\u5316\u7684\u672b\u7aef\u3002\u9650\u5236\u6027\u5185\u5207\u9176<em>Sma<\/em>I \u548c<em>Xma<\/em>\u6211\u8bc6\u522b\u5e8f\u52175\u2032-CCCGGG-3\u2032\uff0c\u4f46\u662f<em>Sma<\/em>I\u5728\u9650\u5236\u6027\u4f4d\u70b9\u7684\u6b63\u4e2d\u95f4\uff085\u2032-CCC\u2193GGG-3\u2032\uff09\u5207\u5272\uff0c\u4ea7\u751f\u5e73\u7aef\uff0c\u4e14<em>Xma<\/em>I\u57285\u2032-C\u2193CCGGG-3\u2032\u5904\u5207\u5272\uff0c\u4ea7\u751f\u7c98\u6027\u672b\u7aef\u3002<\/p>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aae269f753cd&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aae269f753cd\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/09\/RE3-1024x321.png\" alt=\"Restriction endonuclease cleavage site examples\" class=\"wp-image-5003\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<p>\u540c\u5de5\u9176\u4e3a\u5b9e\u9a8c\u8bbe\u8ba1\u63d0\u4f9b\u4e86\u591a\u79cd\u4f18\u52bf\uff1a<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u6027\u80fd\u63d0\u5347<\/strong>\u65b0\u53d1\u73b0\u5e76\u5546\u4e1a\u5316\u7684\u540c\u88c2\u9176\u76f8\u6bd4\u4f20\u7edf\u9176\u66f4\u5177\u6210\u672c\u6548\u76ca\uff0c\u5e76\u4e14\u62e5\u6709\u66f4\u4f18\u8d8a\u7684\u7279\u6027\uff0c\u4f8b\u5982\u66f4\u9ad8\u7684\u7a33\u5b9a\u6027\u548c\u65e0\u661f\u6d3b\u6027\u3002<\/li>\n\n\n\n<li><strong>\u7532\u57fa\u5316\u654f\u611f\u6027<\/strong>\u6709\u4e9b\u540c\u88c2\u9176\u5bf9DNA\u7532\u57fa\u5316\u654f\u611f\uff0c\u800c\u53e6\u4e00\u4e9b\u5219\u4e0d\u654f\u611f\u3002\u8fd9\u4e00\u7279\u6027\u5bf9\u4e8e\u7814\u7a76\u8868\u89c2\u9057\u4f20\u4fee\u9970\u5c24\u4e3a\u6709\u7528\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u5982\u9700\u4e86\u89e3\u66f4\u591a\u4fe1\u606f\uff0c\u8bf7\u8bbf\u95ee\u6211\u4eec\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/spotlight-articles\/digesting-methylated-dna.html?cid=bid_mol_clo_r01_co_cp1613_pjt12393_col000000_0so_blg_il_awa_kt_s18_RestEnzyBlg2#schizomers\" target=\"_blank\" rel=\"noreferrer noopener\">\u7532\u57fa\u5316DNA\u6d88\u5316\u9875\u9762<\/a><\/p>\n\n\n\n<p>\u5f53\u4f60\u770b\u5230\u7c7b\u4f3c\u4e0a\u8ff0\u7684\u9176\u540d\u79f0\u65f6\uff0c<em>Bcu<\/em>I\uff08<em>Spe<\/em>I\uff09\uff0c\u62ec\u53f7\u4e2d\u5217\u51fa\u7684\u9176\uff0c<em>Spe<\/em>\u6211\uff0c\u4ee3\u8868\u4e00\u79cd\u539f\u578b<br>\u2014\u2014\u5373\u7b2c\u4e00\u4e2a\u88ab\u53d1\u73b0\u548c\u9274\u5b9a\u7684\u9650\u5236\u6027\u5185\u5207\u9176\u3002<em>Bcu<\/em>I \u662f\u5728\u539f\u578b\u4e4b\u540e\u53d1\u73b0\u7684\u540c\u5de5\u5f02\u6784\u9176\u3002<em>Spe<\/em>I \u80fd\u8bc6\u522b\u5e76\u5207\u5272\u76f8\u540c\u7684 DNA \u5e8f\u5217\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4f7f\u7528iis\u578b\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176-golden-gate\u514b\u9686\u6280\u672f\">\u4f7f\u7528IIS\u578b\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\uff1aGolden Gate\u514b\u9686\u6280\u672f<\/h2>\n\n\n\n<p>Golden Gate \u7ec4\u88c5\uff0c\u4e5f\u79f0\u4e3a Golden Gate \u514b\u9686\uff0c\u662f\u4e00\u79cd\u5229\u7528 IIS \u578b\u9650\u5236\u6027\u5185\u5207\u9176\u72ec\u7279\u6027\u8d28\u7684\u65b9\u6cd5\u3002\u5728\u8be5\u6280\u672f\u4e2d\u6700\u5e38\u7528\u7684\u9176\u5305\u62ec\uff1a<em>Eco<\/em>31I (<em>Bsa<\/em>I)\uff0c<em>Bpi<\/em>I\uff08<em>Bbs<\/em>I)\uff0c\u4ee5\u53ca<em>Esp<\/em>3I\uff08<em>Bsm<\/em>BI\uff09\u3002<\/p>\n\n\n\n<p>\u7531\u4e8e\u8bc6\u522b\u4f4d\u70b9\u548c\u5207\u5272\u4f4d\u70b9\u662f\u5206\u5f00\u7684\uff0cDNA\u4f1a\u5728\u8ddd\u79bb\u8bc6\u522b\u4f4d\u70b9\u4e00\u5b9a\u7684\u4f4d\u7f6e\u88ab\u5207\u5272\uff0c\u800c\u4e0d\u662f\u5728\u5e8f\u5217\u672c\u8eab\u5185\u90e8\uff0c\u56e0\u6b64\u4ea7\u751f\u7684\u7c98\u6027\u672b\u7aef\u5728\u4e0d\u540c\u7684\u5207\u5272\u4f4d\u70b9\u4e4b\u95f4\u4f1a\u6709\u6240\u4e0d\u540c\u3002\u8fd9\u4f7f\u5f97\u53ef\u4ee5\u7075\u6d3b\u8bbe\u8ba1\u5305\u542b\u591a\u4e2a\u63d2\u5165\u7247\u6bb5\u7684\u6784\u5efa\u4f53\u3002<\/p>\n\n\n\n<p>\u5728Golden Gate\u7ec4\u88c5\u4e2d\uff0c\u63d2\u5165\u7247\u6bb5\u548c\u76ee\u7684\u8f7d\u4f53\u90fd\u542b\u6709\u517c\u5bb9\u7684\u5207\u5272\u4f4d\u70b9\uff0c\u8fd9\u4e9b\u4f4d\u70b9\u80fd\u591f\u751f\u6210\u4e92\u8865\u7684\u7c98\u6027\u672b\u7aef\uff0c\u4ece\u800c\u4f7f\u5404\u4e2a\u7247\u6bb5\u6309\u7167\u9884\u671f\u987a\u5e8f\u8fdb\u884c\u7ec4\u88c5\u3002<\/p>\n\n\n\n<p>\u8bc6\u522b\u4f4d\u70b9\u5728\u76ee\u7684\u8f7d\u4f53\u4e2d\u88ab\u5b9a\u4f4d\u4e8e\u5916\u4fa7\uff08\u5373\u63d2\u5165\u533a\u57df\u5185\uff0c\u800c\u975e\u8f7d\u4f53\u9aa8\u67b6\uff09\uff0c\u800c\u5728\u63d2\u5165\u7247\u6bb5\u4e2d\u5219\u5b9a\u4f4d\u4e8e\u5185\u4fa7\uff08\u5373\u671d\u5411\u5f85\u7ec4\u88c5DNA\uff09\uff0c\u786e\u4fdd\u8fd9\u4e9b\u8bc6\u522b\u4f4d\u70b9\u4e0d\u4f1a\u4fdd\u7559\u5728\u6700\u7ec8\u6784\u5efa\u4f53\u4e2d\u3002\u8fd9\u5141\u8bb8\u6240\u6709\u63d2\u5165\u7247\u6bb5\u548c\u76ee\u7684\u8f7d\u4f53\u540c\u65f6\u7528\u9650\u5236\u6027\u9176\u548cDNA\u8fde\u63a5\u9176\u8fdb\u884c\u5355\u6b21\u53cd\u5e94\u5904\u7406\u3002\u5982\u679c\u63d2\u5165\u7247\u6bb5\u672a\u88ab\u7ec4\u88c5\u5230\u76ee\u7684\u8f7d\u4f53\u4e2d\uff0c\u8bc6\u522b\u4f4d\u70b9\u5c06\u4f1a\u4fdd\u7559\uff0c\u4f7f\u9650\u5236\u6027\u9176\u5c06\u8f7d\u4f53\u7ebf\u6027\u5316\u3002\u7531\u4e8e\u7ebf\u6027\u5316\u7684\u8f7d\u4f53\u5728\u7ec6\u83cc\u8f6c\u5316\u4e2d\u7684\u6548\u7387\u8f83\u4f4e\uff0c\u53ef\u4ee5\u6700\u5927\u7a0b\u5ea6\u51cf\u5c11\u8f6c\u5316\u80cc\u666f\u3002Golden Gate\u514b\u9686\u6280\u672f\u6700\u591a\u53ef\u7ec4\u88c535\u4e2aDNA\u7247\u6bb5<sup>[1]<\/sup>.<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aae269f75bbd&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aae269f75bbd\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/09\/RE4-1024x602.png\" alt=\"Golden Gate cloning\" class=\"wp-image-5006\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u65e0\u7f1dgolden-gate\u7ec4\u88c5\u6280\u5de7\">\u65e0\u7f1dGolden Gate\u7ec4\u88c5\u6280\u5de7<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5728\u5b9e\u9a8c\u5f00\u59cb\u524d\uff0c\u4f7f\u7528SnapGene\u6216Benchling\u7b49\u8f6f\u4ef6\u5de5\u5177\u53ef\u89c6\u5316\u5e76\u786e\u8ba4\u63d2\u5165\u7247\u6bb5\u4e0e\u76ee\u7684\u8f7d\u4f53\u4e2d\u8bc6\u522b\u4f4d\u70b9\u7684\u65b9\u5411\u3002\u786e\u4fdd\u8bc6\u522b\u4f4d\u70b9\u5728\u76ee\u7684\u8f7d\u4f53\u4e2d\u671d\u5916\uff0c\u5728\u63d2\u5165\u7247\u6bb5\u4e2d\u671d\u5185\u3002\u5982\u679c\u65b9\u5411\u6b63\u786e\uff0c\u6d88\u5316\u540e\u8bc6\u522b\u4f4d\u70b9\u5c06\u5728\u8f7d\u4f53\u548c\u63d2\u5165\u7247\u6bb5\u4e0a\u90fd\u88ab\u53bb\u9664\u3002<\/li>\n\n\n\n<li>\u5206\u6790\u4f60\u7684\u63d2\u5165\u7247\u6bb5\u548c\u76ee\u7684\u8f7d\u4f53\u5e8f\u5217\uff0c\u786e\u4fdd\u6ca1\u6709\u5176\u4ed6\u53ef\u80fd\u5e72\u6270\u514b\u9686\u8fc7\u7a0b\u7684\u989d\u5916\u8bc6\u522b\u4f4d\u70b9\u3002<\/li>\n\n\n\n<li>\u6700\u591a\u53ef\u5c0610\u4e2a\u7247\u6bb5\u7ec4\u88c5\u5230\u4e00\u4e2a\u6784\u5efa\u4f53\u4e2d\u3002<\/li>\n\n\n\n<li>\u4f18\u5316\u4f60\u7684\u5b9e\u9a8c\u6761\u4ef6\u3002\u786e\u4fdd\u4f60\u7684\u8fde\u63a5\u7f13\u51b2\u6db2\u542b\u6709ATP\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5e94\u7528-\u690d\u7269\u5de5\u7a0b\u4e2d\u7684golden-gate\u514b\u9686\u6280\u672f\">\u5e94\u7528\uff1a\u690d\u7269\u5de5\u7a0b\u4e2d\u7684Golden Gate\u514b\u9686\u6280\u672f<\/h2>\n\n\n\n<p>\u7531\u4e8e\u5408\u6210\u751f\u7269\u5b66\u7684\u8fdb\u6b65\uff0c\u690d\u7269\u7814\u7a76\u548c\u80b2\u79cd\u5de5\u4f5c\u901a\u8fc7\u4f7f\u7528\u5e8f\u5217\u7279\u5f02\u6027\u6838\u9178\u9176\uff08\u5982TALENs\u3001ZFNs\u548cCRISPR\u2013Cas\u7cfb\u7edf\uff09\u5f97\u5230\u4e86\u663e\u8457\u63d0\u5347\u3002<sup>[2]<\/sup><\/p>\n\n\n\n<p>\u690d\u7269\u57fa\u56e0\u5de5\u7a0b\u7814\u7a76\u901a\u5e38\u6d89\u53ca\u8bbe\u8ba1\u590d\u6742\u7684\u5408\u6210\u6784\u5efa\u4f53\uff0c\u4f8b\u5982\u65b0\u7684\u4ee3\u8c22\u9014\u5f84\u3001\u57fa\u56e0\u8c03\u63a7\u76d2\u6216\u7528\u4e8e\u57fa\u56e0\u7ec4\u7f16\u8f91\u7684\u6784\u5efa\u4f53\u3002<sup>[3,4]<\/sup> \u4f8b\u5982\uff0c\u7528\u4e8e\u57fa\u56e0\u5de5\u7a0b\u7684\u76d2\u901a\u5e38\u5305\u542b\u591a\u4e2a\u63d2\u5165\u7247\u6bb5\uff0c\u8fd9\u4e9b\u7247\u6bb5\u7f16\u7801\u4e00\u4e2a\u6216\u591a\u4e2a\u5411\u5bfcRNA\u4ee5\u53ca\u6240\u9700\u7684\u6838\u9178\u9176\u3002<\/p>\n\n\n\n<p>\u5408\u6210\u9014\u5f84\u6784\u5efa\u9700\u8981\u5bf9\u8bb8\u591a\u8c03\u63a7\u5143\u4ef6\u8fdb\u884c\u5408\u7406\u8bbe\u8ba1\uff0c\u4ee5\u5efa\u7acb\u9057\u4f20\u903b\u8f91\u95e8\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0cGolden Gate\u7ec4\u88c5\u662f\u4e00\u79cd\u6709\u5438\u5f15\u529b\u7684\u5de5\u5177\uff0c\u5b83\u901a\u8fc7\u5141\u8bb8\u5728\u5355\u6b21\u53cd\u5e94\u4e2d\u540c\u65f6\u7ec4\u88c5\u591a\u4e2aDNA\u7247\u6bb5\u5e76\u51cf\u5c11\u5927\u91cf\u7b5b\u9009\u9700\u6c42\uff0c\u7b80\u5316\u4e86\u514b\u9686\u8fc7\u7a0b\uff0c\u4ece\u800c\u63a8\u52a8\u519c\u4e1a\u548c\u751f\u7269\u6280\u672f\u9886\u57df\u7684\u521b\u65b0\u3002<sup>[5]<\/sup><\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aae269f761e2&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aae269f761e2\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/09\/RE5-1024x404.png\" alt=\"Golden Gate cloning in plant science application\" class=\"wp-image-5007\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><em><em>\u56fe\u4f8b\u6539\u7f16\u81eaHahn, Florian\u7b49\u4eba\uff082020\uff09\u3002<sup>[6]<\/sup>\u91c7\u7528\u8bbe\u8ba1\u5de5\u5177Figma\u5236\u4f5c<\/em><\/em><\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5173\u4e8e\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u66f4\u591a\u8d44\u6e90\">\u5173\u4e8e\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176\u7684\u66f4\u591a\u8d44\u6e90<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u82f1\u7ef4\u521b\u5206\u5b50\u751f\u7269\u5b66\u5b66\u9662\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/molecular-cloning\/restriction-enzymes.html\">\u9650\u5236\u6027\u9176\u6559\u80b2<\/a><\/li>\n\n\n\n<li><strong>\u9009\u62e9\u5de5\u5177\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/conventional-restriction-enzymes-thermo-scientific.html\">\u5e38\u89c4\u9650\u5236\u6027\u9176\u9009\u62e9\u5de5\u5177<\/a> &amp; <a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/fastdigest-thermo-scientific\/fastdigest-enzyme-selection-tool.html\">FastDigest\u9650\u5236\u6027\u9176\u9009\u62e9\u5de5\u5177<\/a><\/li>\n\n\n\n<li><strong>\u8ba1\u7b97\u5668\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/double-digest-calculator-thermo-scientific.html\">\u53cc\u91cd\u6d88\u5316\u8ba1\u7b97\u5668<\/a><\/li>\n\n\n\n<li><strong>\u76ee\u5f55\uff1a<\/strong><a href=\"https:\/\/www.thermofisher.com\/search\/results?query=fastdigest%20restriction%20enzymes&amp;focusarea=Search%20All&amp;cid=bid_mol_clo_r01_co_cp1613_pjt12393_col000000_0so_blg_il_awa_kt_s18_RestEnzyBlg3\" target=\"_blank\" rel=\"noreferrer noopener\">\u9650\u5236\u6027\u9176<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.com\/search\/results?query=fastdigest%20restriction%20enzymes&amp;focusarea=Search%20All&amp;cid=bid_mol_clo_r01_co_cp1613_pjt12393_col000000_0so_blg_il_awa_kt_s18_RestEnzyBlg4\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u63a2\u7d22\u8d5b\u9ed8\u98de\u4e16\u5c14\u7684\u9650\u5236\u6027\u6838\u9178\u5185\u5207\u9176<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u53c2\u8003\u6587\u732e\">\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Pryor, J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., &amp; Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design.&nbsp;<em>PloS one<\/em>,&nbsp;<em>15<\/em>(9), e0238592. <a href=\"https:\/\/doi.org\/10.1371\/journal.pone.0238592\">https:\/\/doi.org\/10.1371\/journal.pone.0238592<\/a><\/li>\n\n\n\n<li>Osakabe, Y., &amp; Osakabe, K. (2015). Genome editing with engineered nucleases in plants.&nbsp;<em>Plant &amp; cell physiology<\/em>,&nbsp;<em>56<\/em>(3), 389\u2013400. <a href=\"https:\/\/doi.org\/10.1093\/pcp\/pcu170\">https:\/\/doi.org\/10.1093\/pcp\/pcu170<\/a><\/li>\n\n\n\n<li>Van Eck J. (2020). Applying gene editing to tailor precise genetic modifications in plants.&nbsp;<em>The Journal of biological chemistry<\/em>,&nbsp;<em>295<\/em>(38), 13267\u201313276. <a href=\"https:\/\/doi.org\/10.1074\/jbc.REV120.010850\">https:\/\/doi.org\/10.1074\/jbc.REV120.010850<\/a><\/li>\n\n\n\n<li>Pan, C., Wu, X., Markel, K., Malzahn, A. A., Kundagrami, N., Sretenovic, S., Zhang, Y., Cheng, Y., Shih, P. M., &amp; Qi, Y. (2021). CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants.&nbsp;<em>Nature plants<\/em>,&nbsp;<em>7<\/em>(7), 942\u2013953. https:\/\/doi.org\/10.1038\/s41477-021-00953-7<\/li>\n\n\n\n<li>Karimi, M., &amp; Jacobs, T. B. (2021). GoldenGateway: A DNA Assembly Method for Plant Biotechnology.&nbsp;<em>Trends in plant science<\/em>,&nbsp;<em>26<\/em>(1), 95\u201396. <a href=\"https:\/\/doi.org\/10.1016\/j.tplants.2020.10.004\">https:\/\/doi.org\/10.1016\/j.tplants.2020.10.004<\/a><\/li>\n\n\n\n<li>Hahn, F., Korolev, A., Sanjurjo Loures, L., &amp; Nekrasov, V. (2020). A modular cloning toolkit for genome editing in plants.&nbsp;<em>BMC plant biology<\/em>,&nbsp;<em>20<\/em>(1), 179. <a href=\"https:\/\/doi.org\/10.1186\/s12870-020-02388-2\">https:\/\/doi.org\/10.1186\/s12870-020-02388-2<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>##<\/p>\n\n\n\n<p><em>\u00a9 2025 \u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280\u516c\u53f8\u7248\u6743\u6240\u6709\u3002\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u6240\u6709\u5546\u6807\u5747\u4e3a\u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280\u53ca\u5176\u5b50\u516c\u53f8\u7684\u8d22\u4ea7\u3002\u5982\u6709\u8bf4\u660e\u5219\u4f8b\u5916\u3002<\/em><\/p>\n\n\n\n<p><em><em>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\uff0c\u4e0d\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" 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