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3D \u764c\u75c7\u7c7b\u5668\u5b98\u7684\u5f62\u6001\u5b66\u7279\u5f81<\/strong><\/strong><\/h2>\n\n\n\n

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\"Time<\/figure>\n<\/div>\n\n\n

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\"Time<\/figure>\n<\/div>\n\n\n

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\"Time<\/figure>\n<\/div>\n\n\n

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\u7c7b\u5668\u5b98\u57f9\u517b\u6700\u4f73\u5b9e\u8df5\u6307\u5357<\/strong><\/strong><\/h2>\n\n\n\n

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\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u5236\u5907<\/strong><\/h3>\n\n\n\n
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  1. \u4f7f\u7528OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u5957\u88c5\uff1a<\/strong>OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u7ecf\u8fc7\u4f18\u5316\uff0c\u9002\u7528\u4e8e\u6269\u589e\u60a3\u8005\u6765\u6e90\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u3002OncoPro\u5957\u88c5\u7b80\u5316\u4e86\u764c\u75c7\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u7684\u5236\u5907\u6d41\u7a0b\uff0c\u65e0\u9700\u91cd\u65b0\u914d\u5236\u3001\u5206\u88c5\u548c\u6df7\u5408\u5e38\u89c1\u4e8e\u81ea\u5236\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u4e2d\u7684\u5404\u7c7b\u751f\u957f\u56e0\u5b50\u548c\u8865\u5145\u5242\u3002<\/li>\n<\/ol>\n\n\n
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    \"Materials<\/figure>\n<\/div>\n\n\n

    \u56fe5\uff1a\u51c6\u5907OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\uff08\u4e0d\u542b\u6297\u751f\u7d20\uff09\u53ca\u4ee3\u8868\u6027\u81ea\u5236\u57f9\u517b\u57fa\u6240\u9700\u7684\u6750\u6599\u3002\u5bf9\u4e8e\u81ea\u5236\u57f9\u517b\u57fa\uff0c\u5c55\u793a\u4e86\u91cd\u60ac\u3001\u5206\u88c5\u53ca\u50a8\u5b58\u5404\u4e2a\u751f\u957f\u56e0\u5b50\u548c\u8865\u5145\u5242\u6240\u9700\u7684\u6750\u6599\u3002<\/p>\n\n\n\n

      \n
    1. \u57f9\u517b\u57fa\u7ec4\u5206\u7684\u50a8\u5b58\uff1a<\/strong>\u5982\u6709\u9700\u8981\uff0c\u5f53\u6bcf\u6b21\u4f7f\u7528\u91cf\u5c11\u4e8e500\u6beb\u5347\u65f6\uff0c\u53ef\u5c06OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u7ec4\u5206\u8fdb\u884c\u5206\u88c5\u3002OncoPro\u57fa\u7840\u57f9\u517b\u57fa\u548cOncoPro BSA\u5e94\u50a8\u5b58\u57284\u00b0C\uff0c\u53ef\u76f4\u63a5\u4ece\u539f\u59cb\u7ec4\u5206\u74f6\u4e2d\u53d6\u7528\u3002B-27\u8865\u5145\u5242\u53ef\u5728\u5ba4\u6e29\u4e0b\u89e3\u51bb\uff0c\u5206\u88c5\u540e\u518d\u6b21\u51b7\u51bb\u5e76\u50a8\u5b58\u4e8e-20\u00b0C\u3002OncoPro\u8865\u5145\u5242\u5e94\u572837\u00b0C\u6c34\u6d74\u4e2d\u89e3\u51bb\uff0c\u5206\u88c5\u540e\u5feb\u901f\u51b7\u51bb\u4e8e-80\u00b0C\u8fc7\u591c\uff0c\u7136\u540e\u50a8\u5b58\u4e8e-20\u00b0C\u3002\u5404\u7ec4\u5206\u7684\u5206\u88c5\u54c1\u53ef\u4fdd\u5b58\u81f3\u5176\u6709\u6548\u671f\u3002\u4e0d\u5efa\u8bae\u5c06\u5b8c\u6574\u57f9\u517b\u57fa\u914d\u597d\u540e\u518d\u51b7\u51bb\u4fdd\u5b58\u3002<\/li>\n\n\n\n
    2. \u57f9\u517b\u57fa\u5236\u5907\u9891\u7387\uff1a<\/strong>\u8bf7\u914d\u5236\u5b8c\u6574\u57f9\u517b\u57fa\uff0c\u5e76\u57287\u5929\u5185\u4f7f\u7528\u4ee5\u83b7\u5f97\u6700\u4f73\u6548\u679c\u3002\u4e0d\u4f7f\u7528\u65f6\uff0c\u5c06\u5176\u50a8\u5b58\u57284\u00b0C\u5e76\u907f\u514d\u5149\u7167\u3002<\/li>\n\n\n\n
    3. \u4f7f\u7528\u524d\u52a0\u6e29\u5904\u7406\uff1a<\/strong>\u907f\u514d\u4f7f\u752837\u00b0C\u6c34\u6d74\u52a0\u6e29\u3002\u5982\u9700\u52a0\u6e29\u5df2\u914d\u597d\u7684\u57f9\u517b\u57fa\uff0c\u5efa\u8bae\u5c06\u5176\u7f6e\u4e8e\u5ba4\u6e29\u4e0b\uff08\u5b9e\u9a8c\u53f0\u6216\u751f\u7269\u5b89\u5168\u67dc\u5185\uff09\u7ea61\u5c0f\u65f6\uff0c\u5e76\u907f\u514d\u5149\u7167\u3002\u4e0d\u4f7f\u7528\u65f6\uff0c\u5c06\u5176\u50a8\u5b58\u57284\u00b0C\u3002<\/li>\n<\/ol>\n\n\n\n

      \u764c\u7ec6\u80de\u64ad\u79cd<\/strong><\/h3>\n\n\n\n

      \u89e3\u79bb\u540e\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u7ec6\u80de\u53ef\u4ee5\u5728\u6c34\u51dd\u80f6\uff08\u4f8b\u5982\uff0cGeltrex\uff09\u4e2d\u8fdb\u884c\u5d4c\u5165\u5f0f\u57f9\u517b\uff0c\u4e5f\u53ef\u4ee5\u5728\u60ac\u6d6e\u57f9\u517b\u4e2d\u8fdb\u884c\uff0c\u6b64\u65f6\u4f1a\u5411\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0\u7a00\u91ca\u7684\u57fa\u5e95\u819c\u63d0\u53d6\u7269\uff08\u4f8b\u5982\uff0c\u4f53\u79ef\u6bd4\u4e3a2%\u7684Geltrex\uff09\uff0c\u4ee5\u63d0\u4f9b\u7ec6\u80de\u5916\u57fa\u8d28\u4fe1\u53f7\u3002<\/p>\n\n\n\n

        \n
      1. \u60ac\u6d6e\u57f9\u517b\u4e0e\u5d4c\u5165\u5f0f\u57f9\u517b\uff1a<\/strong>\u5728\u521d\u59cb\u80bf\u7624\u7c7b\u5668\u5b98\u884d\u751f\u8fc7\u7a0b\u4e2d\u901a\u5e38\u66f4\u503e\u5411\u4e8e\u91c7\u7528\u5d4c\u5165\u5f0f\u6761\u4ef6\u57f9\u517b\uff08\u8be6\u89c1\u6211\u4eec\u7684\u884d\u751f\u5e94\u7528\u8bf4\u660e<\/a>\u83b7\u53d6\u66f4\u591a\u4fe1\u606f\uff09\uff0c\u800c\u60ac\u6d6e\u57f9\u517b\u5219\u4fbf\u4e8e\u5b9e\u9a8c\u89c4\u6a21\u5316\u6269\u589e\u3002\u5728\u60ac\u6d6e\u72b6\u6001\u4e0b\u7684\u57f9\u517b\u53ef\u80fd\u9700\u8981\u66f4\u9891\u7e41\u5730\u5206\u5272\uff0c\u56e0\u4e3a\u81ea\u7531\u6f02\u6d6e\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u805a\u96c6\u4f1a\u5bfc\u81f4\u5176\u5f62\u6210\u8f83\u5927\u7684\u7c7b\u5668\u5b98\u901f\u5ea6\u5feb\u4e8e\u5d4c\u5165\u5f0f\u57f9\u517b\uff08\u53c2\u89c1\u4e0a\u65b9\u56fe3\u548c\u56fe4\u4e2dHuLu051421\u80bf\u7624\u7c7b\u5668\u5b98\u7cfb\u5728\u60ac\u6d6e\u4e0e\u5d4c\u5165\u5f0f\u57f9\u517b\u4e2d\u7684\u5bf9\u6bd4\uff09\u3002<\/li>\n<\/ol>\n\n\n
        \n
        \"Images<\/figure>\n<\/div>\n\n\n

        \u56fe6\uff1aHuCo1044\u7ed3\u76f4\u80a0\u80bf\u7624\u7c7b\u5668\u5b98\u5728OncoPro Tumoroid Culture Medium\uff08\u60ac\u6d6e\u57f9\u517b\u683c\u5f0f\uff09\u4e2d\u64ad\u79cd\u540e\u7b2c7\u5929\uff0c\u5728\u4e0d\u540c\u653e\u5927\u500d\u6570\u4e0b\u7684\u56fe\u50cf\u3002\u5728\u60ac\u6d6e\u57f9\u517b\u4e2d\uff0c\u80bf\u7624\u7c7b\u5668\u5b98\u5728\u4ecb\u8d28\u4e2d\u81ea\u7531\u6f02\u6d6e\u65f6\u5f62\u6210\u4e09\u7ef4\u7ec6\u80de\u7ed3\u6784\u3002<\/p>\n\n\n\n

          \n
        1. BME\u7684\u6dfb\u52a0\uff1a<\/strong>\u5728\u60ac\u6d6e\u57f9\u517b\u4e2d\u4f7f\u75282%\uff08v\/v\uff09\u6eb6\u89e3\u7684BME\uff0c\u4ee5\u7ef4\u6301\u5177\u6709\u751f\u7406\u76f8\u5173\u6027\u7684\u7ec6\u80de-ECM\u76f8\u4e92\u4f5c\u7528\u3002\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u5bf9\u4e8e\u7ef4\u6301\u80bf\u7624\u7c7b\u5668\u5b98\u7684\u9876\u7aef-\u57fa\u5e95\u6781\u6027\u81f3\u5173\u91cd\u8981\u3002\u4e3a\u4e86\u83b7\u5f97\u6700\u4f73\u6548\u679c\uff0c\u5e94\u5728\u5c06\u7ec6\u80de\u548c\u4ecb\u8d28\u52a0\u5165\u5230\u57f9\u517b\u5bb9\u5668\u540e\uff0c\u5c06\u51b7BME\u6ef4\u52a0\u5230\u4ecb\u8d28\u4e2d\u3002<\/li>\n\n\n\n
        2. ROCK\u6291\u5236\u5242\u7684\u4f7f\u7528\uff1a<\/strong>\u5728\u521d\u59cb\u64ad\u79cd\u65f6\uff0c\u5728\u57f9\u517b\u57fa\u4e2d\u8865\u514510 \u00b5M Y-27632\uff0c\u4ee5\u652f\u6301\u5355\u7ec6\u80de\u7684\u5065\u5eb7\u3002\u6211\u4eec\u901a\u5e38\u5728\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u8fc7\u7a0b\u4e2d\u6301\u7eed\u6dfb\u52a0\u8be5\u5316\u5408\u7269\uff0c\u4f46\u5728\u64ad\u79cd\u540e2-3\u5929\u8fdb\u884c\u57f9\u517b\u5582\u517b\u65f6\u66f4\u6362\u57f9\u517b\u57fa\uff0c\u53ef\u4ee5\u9009\u62e9\u4e0d\u518d\u6dfb\u52a0\u3002<\/li>\n<\/ol>\n\n\n\n

          \u80bf\u7624\u7c7b\u5668\u5b98\u5582\u517b<\/strong><\/h3>\n\n\n\n

          \u5e94\u5b9a\u671f\u5582\u517b\u80bf\u7624\u7c7b\u5668\u5b98\uff0c\u4ee5\u53bb\u9664\u4ee3\u8c22\u5e9f\u7269\u5e76\u63d0\u4f9b\u751f\u957f\u6240\u9700\u7684\u8425\u517b\u3002\u5728\u5305\u57cb\u57f9\u517b\u4e2d\uff0c\u901a\u8fc7\u79fb\u9664\u7528\u8fc7\u7684\u57f9\u517b\u57fa\u5e76\u8986\u76d6\u65b0\u9c9c\u7684OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u6765\u8fdb\u884c\u5582\u517b\u3002\u5728\u60ac\u6d6e\u57f9\u517b\u4e2d\uff0c\u5c06\u7ec6\u80de\u548c\u57f9\u517b\u57fa\u4ece\u677f\u6216\u74f6\u4e2d\u53d6\u51fa\u5e76\u79bb\u5fc3\uff0c\u5f03\u53bb\u4e0a\u6e05\u6db2\uff0c\u7136\u540e\u5c06\u80bf\u7624\u7c7b\u5668\u5b98\u91cd\u65b0\u60ac\u6d6e\u4e8e\u65b0\u9c9c\u57f9\u517b\u57fa\u4e2d\u3002<\/p>\n\n\n\n

            \n
          1. \u5582\u517b\u9891\u7387\uff1a<\/strong>\u5efa\u8bae\u6bcf2-3\u5929\u8fdb\u884c\u4e00\u6b21\u5b8c\u5168\u66f4\u6362\u57f9\u517b\u57fa\uff0c\u4ee5\u786e\u4fdd\u6700\u4f73\u751f\u957f\u3002\u5982\u679c\u672a\u80fd\u6bcf2-3\u5929\u66f4\u6362\uff0c\u4ee3\u8c22\u5e9f\u7269\u79ef\u7d2f\u4f1a\u5bf9\u7ec6\u80de\u6d3b\u6027\u4ea7\u751f\u8d1f\u9762\u5f71\u54cd\uff0c\u5e76\u53ef\u80fd\u5bfc\u81f4\u6574\u4e2a\u57f9\u517b\u5d29\u6e83\u3002\u6211\u4eec\u5efa\u8bae\u5728\u5582\u517b\u6216\u4f20\u4ee3\u5f53\u5929\u65b0\u9c9c\u8865\u514510 \u00b5M Y-27632\u81f3\u57f9\u517b\u57fa\u4e2d\u3002<\/li>\n\n\n\n
          2. \u76d1\u6d4b\u57f9\u517b\u57fa\u989c\u8272\uff1a<\/strong>OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u56e0\u5176\u7565\u9178\u6027\u7684pH\u503c\uff0c\u989c\u8272\u53ef\u80fd\u6bd4\u5176\u4ed6\u57f9\u517b\u57fa\u66f4\u504f\u9ec4\u3002\u968f\u7740\u80bf\u7624\u7c7b\u5668\u5b98\u6269\u589e\u5e76\u4ee3\u8c22\u4ecb\u8d28\u6210\u5206\uff0c\u4ecb\u8d28\u4f1a\u8fdb\u4e00\u6b65\u9178\u5316\uff0c\u5f53\u7ec6\u80de\u63a5\u8fd1\u4f20\u4ee3\u65f6\uff0c\u4ecb\u8d28\u770b\u8d77\u6765\u5c24\u5176\u504f\u9ec4\u3002\u9700\u8981\u9891\u7e41\u76d1\u6d4b\uff08\u6bcf2-3\u5929\uff09\u4ee5\u5224\u65ad\u4f55\u65f6\u5206\u5272\u57f9\u517b\u3002\u5982\u679c\u5df2\u5b9a\u671f\u66f4\u6362\u4ecb\u8d28\u4e14\u80bf\u7624\u7c7b\u5668\u5b98\u76f4\u5f84\u5904\u4e8e100-300 \u00b5m\u8303\u56f4\u5185\uff0c\u5219\u9ec4\u8272\u4e0d\u662f\u503c\u5f97\u62c5\u5fe7\u7684\u95ee\u9898\u3002<\/li>\n<\/ol>\n\n\n
            \n
            \"Examples<\/figure>\n<\/div>\n\n\n

            \u56fe7\uff1a\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u4e2d\u7684\u4ecb\u8d28\u989c\u8272\u793a\u4f8b\u3002\u56fe\u4e2d\u4e3a\u4e09\u53eaT25\u74f6\uff0c\u5728\u5582\u517b\u540e2-3\u5929\u62cd\u6444\uff0c\u90fd\u542b\u6709\u6b63\u5e38\u6269\u589e\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u3002\u5f53\u80bf\u7624\u7c7b\u5668\u5b98\u6570\u91cf\u548c\u4f53\u79ef\u589e\u52a0\u3001\u63a5\u8fd1\u4f20\u4ee3\u65f6\uff0c\u4ecb\u8d28\u53ef\u80fd\u53d8\u5f97\u66f4\u52a0\u504f\u9ec4\u3002<\/p>\n\n\n\n

              \n
            1. BME\u8865\u52a0\uff1a<\/strong>\u5728\u60ac\u6d6e\u57f9\u517b\u8fc7\u7a0b\u4e2d\uff0c\u4e00\u90e8\u5206BME\u4f1a\u5728\u80bf\u7624\u7c7b\u5668\u5b98\u5582\u517b\u65f6\u6d41\u5931\u3002\u4e3a\u8865\u5145\u4e22\u5931\u7684BME\u5e76\u4fdd\u6301\u5176\u76ca\u5904\uff0c\u53ef\u52a0\u51651%\uff08v\/v\uff09\u7684BME\uff08\u5982Geltrex<\/a>\uff09\u5728\u6bcf\u6b21\u6362\u6db2\u65f6\u52a0\u5165\u57f9\u517b\u57fa\u4e2d\u3002BME\u5e94\u5728\u7ec6\u80de\u548c\u57f9\u517b\u57fa\u63a5\u79cd\u5230\u57f9\u517b\u5bb9\u5668\u540e\u518d\u6dfb\u52a0\uff0c\u800c\u4e0d\u662f\u5728\u5582\u517b\u8fc7\u7a0b\u4e2d\u4e0e\u7ec6\u80de\u9884\u5148\u6df7\u5408\u4e8e\u9525\u5f62\u7ba1\u4e2d\u3002<\/li>\n<\/ol>\n\n\n\n

              \u764c\u7c7b\u7c7b\u5668\u5b98\u7684\u4f20\u4ee3<\/strong><\/h3>\n\n\n\n

              \u9274\u4e8e\u4e0d\u540c\u4f9b\u4f53\u4e4b\u95f4\u5f62\u6001\u5404\u5f02\uff0c\u5bc6\u5207\u76d1\u63a7\u80bf\u7624\u7c7b\u5668\u5b98\u7684\u57f9\u517b\u8fc7\u7a0b\u81f3\u5173\u91cd\u8981\uff0c\u5c24\u5176\u662f\u5728\u521d\u671f\u9636\u6bb5\u3002\u7531\u4e8e\u4e0d\u540c\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u5177\u6709\u72ec\u7279\u7684\u5f62\u6001\u5e76\u4ee5\u4e0d\u540c\u901f\u5ea6\u6269\u589e\uff0c\u76d1\u6d4b\u6709\u52a9\u4e8e\u6700\u5927\u5316\u6210\u529f\u7387\uff0c\u5e76\u4e3a\u4f55\u65f6\u8fdb\u884c\u4e9a\u57f9\u517b\u63d0\u4f9b\u5173\u952e\u4fe1\u606f\u3002<\/p>\n\n\n\n

                \n
              1. \u4f20\u4ee3\u7684\u6700\u4f73\u76f4\u5f84\uff1a<\/strong>\u6211\u4eec\u5efa\u8bae\u5f53\u57f9\u517b\u4e2d\u7684\u5927\u578b\u80bf\u7624\u7c7b\u5668\u5b98\u76f4\u5f84\u8fbe\u5230100\u2013300\u5fae\u7c73\u65f6\u8fdb\u884c\u4f20\u4ee3\uff08\u901a\u5e38\u6bcf6-10\u5929\u4e00\u6b21\uff0c\u4f46\u90e8\u5206\u57f9\u517b\u7269\u53ef\u57285\u201314\u5929\u5185\u5206\u5272\uff09\u3002\u5927\u591a\u6570\u80bf\u7624\u7c7b\u5668\u5b98\u7cfb\u5728\u51c6\u5907\u4e9a\u57f9\u517b\u65f6\u76f4\u5f84\u53ef\u8fbe200-300\u5fae\u7c73\uff0c\u4f46\u67d0\u4e9b\u7cfb\u53ef\u80fd\u4ec5\u751f\u957f\u81f3\u7ea6100\u5fae\u7c73\u3002\u8fc7\u65e9\u4f20\u4ee3\u4f1a\u5bf9\u5b58\u6d3b\u7387\u4ea7\u751f\u8d1f\u9762\u5f71\u54cd\uff0c\u5e76\u5bfc\u81f4\u8fc7\u5ea6\u89e3\u79bb\u4e3a\u5355\u4e2a\u7ec6\u80de\uff0c\u4ece\u800c\u589e\u52a0\u5931\u5de2\u51cb\u4ea1\uff08anoikis\uff09\u7684\u98ce\u9669\u3002\u5ef6\u8fdf\u4f20\u4ee3\u5219\u53ef\u80fd\u5bfc\u81f4\u574f\u6b7b\u6838\u5fc3\u5f62\u6210\u3001\u89e3\u79bb\u6548\u679c\u4e0d\u4f73\u3001\u4ee3\u8c22\u5e9f\u7269\u79ef\u7d2f\u4ee5\u53ca\u6700\u7ec8\u7684\u57f9\u517b\u5d29\u6e83\u3002\u4e0b\u56fe\u4e2d\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u4ee3\u8868\u4e86\u540c\u4e00\u764c\u75c7\u7c7b\u578b\uff08\u672c\u4f8b\u4e3a\u7ed3\u76f4\u80a0\u764c\uff09\u5185\u53ef\u80fd\u51fa\u73b0\u7684\u5f62\u6001\u53d8\u5316\u3002<\/li>\n<\/ol>\n\n\n
                \n
                \"Examples<\/figure>\n<\/div>\n\n\n

                \u56fe8\uff1a\u4e09\u79cd\u7ed3\u76f4\u80a0\u764c\u80bf\u7624\u7c7b\u5668\u5b98\u7cfb\u572810\u500d\u30014\u500d\u548c1.25\u500d\u663e\u5fae\u955c\u4e0b\u51c6\u5907\u4f20\u4ee3\u65f6\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff0c\u6bd4\u4f8b\u5c3a\u5df2\u6807\u6ce8\u3002<\/p>\n\n\n\n

                  \n
                1. \u6d17\u6da4\u6b65\u9aa4\uff1a<\/strong>\u4f7f\u7528\u51b7\u7f13\u51b2\u6db2\uff08\u5982\u51b7DMEM\/F12\u3001DPBS -\/-\uff09\u6253\u6563\u805a\u5408\u540e\u7684BME\uff0c\u5e76\u5c06\u7ec6\u80de\u4eceBME\u7f51\u7edc\u4e2d\u5206\u79bb\u51fa\u6765\u3002\u8fd9\u4e00\u6b65\u5bf9\u4e8e\u786e\u4fdd\u6d88\u5316\u9176\u80fd\u6709\u6548\u89e3\u79bb\u80bf\u7624\u7c7b\u5668\u5b98\u81f3\u5173\u91cd\u8981\u3002<\/li>\n\n\n\n
                2. \u6d88\u5316\u8bd5\u5242\uff1a<\/strong>\u6839\u636e\u6211\u4eec\u7684\u7ecf\u9a8c\uff0c\u4f7f\u7528StemPro Accutase<\/a>\u8fdb\u884c\u5206\u79bb\u80fd\u591f\u83b7\u5f97\u7a33\u5b9a\u7684\u751f\u957f\u901f\u7387\u3002\u8fd9\u79cd\u9176\u80fd\u591f\u9ad8\u6548\u5730\u5c06\u80bf\u7624\u7c7b\u5668\u5b98\u89e3\u79bb\u4e3a\u5355\u4e2a\u7ec6\u80de\u548c\u5c0f\u578b\u7ec6\u80de\u56e2\uff0c\u540c\u65f6\u4e0d\u4f1a\u5f71\u54cd\u7ec6\u80de\u6d3b\u6027\u3002StemPro Accutase\u7b49\u5206\u8bd5\u5242\u5e94\u50a8\u5b58\u4e8e-20\u00b0C\uff0c\u5e76\u5728\u4f7f\u7528\u5f53\u5929\u4e8e\u5ba4\u6e29\u4e0b\u89e3\u51bb\u3002\u5176\u4ed6\u89e3\u79bb\u8bd5\u5242\u5982TrypLE<\/a>\u4e5f\u53ef\u6839\u636e\u7814\u7a76\u7684\u5177\u4f53\u9700\u6c42\u548c\u7c7b\u5668\u5b98\u6a21\u578b\u7684\u7279\u6027\u9009\u62e9\u4f7f\u7528\u3002\u6211\u4eec\u5efa\u8bae\u5728\u5b75\u80b2\u8fc7\u7a0b\u4e2d\u5411\u89e3\u79bb\u8bd5\u5242\u4e2d\u8865\u514510 \u00b5M Y-27632\uff0c\u4ee5\u652f\u6301\u5355\u4e00\u5316\u80bf\u7624\u7c7b\u5668\u5b98\u7ec6\u80de\u7684\u5065\u5eb7\u3002<\/li>\n\n\n\n
                3. \u89e3\u79bb\u6e29\u5ea6\uff1a<\/strong>\u6211\u4eec\u5efa\u8bae\u572837\u00b0C\u6761\u4ef6\u4e0b\uff0c\u7528\u8865\u5145\u4e8610 \u00b5M Y-27632\u7684StemPro Accutase\u5bf9\u80bf\u7624\u7c7b\u5668\u5b98\u8fdb\u884c\u89e3\u79bb\u3002\u6b64\u64cd\u4f5c\u53ef\u572837\u00b0C\u6c34\u6d74\u4e2d\u8fdb\u884c\uff0c\u4e5f\u53ef\u5c06CO2\u8010\u6447\u5e8a\u653e\u7f6e\u4e8e37\u00b0C\u30015% CO2\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\uff0c\u5e76\u8bbe\u7f6e\u4e3a120 rpm\u3002\u4e0d\u5efa\u8bae\u4f7f\u7528\u52a0\u70ed\u73e0\u6d74\u8fdb\u884c\u89e3\u79bb\u3002\u5728\u6c34\u6d74\u4e2d\u8fdb\u884c\u89e3\u79bb\u65f6\uff0c\u6bcf2-3\u5206\u949f\u8f7b\u8f7b\u65cb\u8f6c\u542b\u6709\u80bf\u7624\u7c7b\u5668\u5b98\u60ac\u6db2\u7684\u9525\u5f62\u7ba1\uff0c\u4ee5\u4fdd\u6301\u5176\u5728\u89e3\u79bb\u8bd5\u5242\u4e2d\u7684\u5747\u5300\u5206\u6563\u3002<\/li>\n\n\n\n
                4. \u5439\u6253\uff1a<\/strong>\u7ecf\u8fc7\u4e0e\u89e3\u79bb\u8bd5\u5242\u5b75\u80b2\u540e\uff0c\u7528P1000\u79fb\u6db2\u5668\u5438\u5934\u5c06\u7ec6\u80de\u60ac\u6db2\u53cd\u590d\u5439\u625320\u6b21\uff0c\u4ee5\u8fdb\u4e00\u6b65\u5206\u6563\u80bf\u7624\u7c7b\u5668\u5b98\u3002\u4e0d\u8981\u4f7f\u7528\u5bbd\u53e3\u79fb\u6db2\u5668\u5438\u5934\uff0c\u56e0\u4e3a\u5b83\u4eec\u65e0\u6cd5\u63d0\u4f9b\u8db3\u591f\u7684\u673a\u68b0\u529b\u6765\u7834\u574f\u4e09\u7ef4\u7ed3\u6784\u3002\u5728\u6b64\u6b65\u9aa4\u4e4b\u524d\uff0c\u79fb\u6db2\u5668\u5438\u5934\u5e94\u9884\u5148\u6da6\u6e7f\uff08\u901a\u8fc7\u5439\u6253\u65e0\u7ec6\u80de\u7684OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\uff09\uff0c\u4ee5\u907f\u514d\u7ec6\u80de\u7c98\u9644\u5728\u5438\u5934\u4e0a\u3002\u5f53\u7528P1000\u79fb\u6db2\u5668\u5438\u5934\u5439\u6253\u540e\uff0c\u7ec6\u80de\u6eb6\u6db2\u53d8\u6d51\u6d4a\u5373\u8868\u660e\u89e3\u79bb\u5145\u5206\u3002\u8089\u773c\u4e0d\u5e94\u770b\u5230\u4efb\u4f55\u7ec6\u80de\u56e2\u5757\uff0c\u4e14\u5728\u4f20\u4ee3\u540e\u4e0d\u5e94\u6709\u8fb9\u754c\u6e05\u6670\u3001\u5b8c\u6574\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u6b8b\u7559\u3002<\/li>\n<\/ol>\n\n\n
                  \n
                  \"Images<\/figure>\n<\/div>\n\n\n

                  \u56fe9\uff1a\u9525\u5f62\u7ba1\u5185\u6709\u6548\u89e3\u79bb\u7684\u80bf\u7624\u7c7b\u5668\u5b98\uff08\u4e0a\uff09\u548c\u9700\u8981\u5728StemPro Accutase\u4e2d\u5ef6\u957f\u5b75\u80b2\u65f6\u95f4\u7684\u80bf\u7624\u7c7b\u5668\u5b98\uff08\u4e0b\uff09\u7684\u56fe\u7247\uff0c\u8fd9\u4e9b\u53ef\u7528\u8089\u773c\u89c2\u5bdf\u5230\u3002<\/p>\n\n\n\n

                    \n
                  1. \u5ef6\u957f\u5b75\u80b2\uff1a<\/strong>\u5982\u6709\u7591\u95ee\uff0c\u53ef\u5728\u89e3\u79bb\u8bd5\u5242\u4e2d\u518d\u5b75\u80b25-10\u5206\u949f\uff0c\u5e76\u518d\u6b21\u5439\u6253\uff0c\u7136\u540e\u7a00\u91ca\u5e76\u8ba1\u6570\u3002\u6211\u4eec\u5efa\u8bae\u6bcf\u6b21\u589e\u52a02\u5206\u949f\u89e3\u79bb\u65f6\u95f4\uff0c\u603b\u65f6\u957f\u6700\u591a\u53ef\u8fbe20\u5206\u949f\uff0c\u76f4\u5230\u7ec6\u80de\u5145\u5206\u89e3\u79bb\u4e3a\u6b62\u3002<\/li>\n\n\n\n
                  2. \u96be\u4ee5\u89e3\u79bb\u7684\u7ec6\u80de\u7cfb\u9707\u8361\u5904\u7406\uff1a<\/strong>\u5bf9\u4e8e\u7279\u522b\u96be\u4ee5\u6709\u6548\u89e3\u79bb\u7684\u7ec6\u80de\u7cfb\uff0c\u53ef\u4ee5\u8003\u8651\u5728\u4e0e\u89e3\u79bb\u8bd5\u5242\u5b75\u80b2\u671f\u95f4\uff0c\u6bcf\u4e24\u5206\u949f\u5bf9\u5c01\u95ed\u7ba1\u8fdb\u884c\u77ed\u6682\u9707\u8361\uff08\u4f8b\u5982\u4f7f\u7528FisherbrandTMMini Vortex Mixer\u9707\u83612\u79d2\uff09\u3002<\/li>\n\n\n\n
                  3. \u7ec6\u80de\u8ba1\u6570\uff1a<\/strong>\u6211\u4eec\u5efa\u8bae\u6bcf\u6b21\u4f20\u4ee3\u90fd\u8fdb\u884c\u7ec6\u80de\u8ba1\u6570\uff0c\u4ee5\u66f4\u597d\u5730\u63a7\u5236\u64ad\u79cd\u7684\u4e00\u81f4\u6027\uff0c\u5e76\u91cf\u5316\u5404\u6a21\u578b\u7684\u500d\u589e\u65f6\u95f4\u53ca\u7d2f\u8ba1\u7fa4\u4f53\u500d\u589e\u6570\u3002\u5728\u8ba1\u6570\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u80fd\u4f1a\u51fa\u73b0\u5c0f\u578b\u7ec6\u80de\u56e2\u5757\u3002\u4e3a\u4e86\u83b7\u5f97\u51c6\u786e\u6570\u636e\uff0c\u5e94\u8ba1\u5165\u8fd9\u4e9b\u56e2\u5757\u4e2d\u7684\u5355\u4e2a\u7ec6\u80de\uff08\u89c1\u56fe10\uff09\u3002\u53ef\u4ee5\u5728\u57fa\u4e8e\u56fe\u50cf\u7684\u81ea\u52a8\u5316\u7ec6\u80de\u8ba1\u6570\u4eea\u4e0a\u5b9e\u65bd\u53bb\u56e2\u7b97\u6cd5\uff0c\u4ee5\u51c6\u786e\u8ba1\u6570\u5c0f\u56e2\u5757\u4e2d\u7684\u7ec6\u80de\u3002\u6211\u4eec\u53d1\u73b0\uff0c\u5c06\u6700\u5c0f\u7269\u4f53\u76f4\u5f84\u8bbe\u4e3a8\u5fae\u7c73\u3001\u6700\u5927\u7269\u4f53\u76f4\u5f84\u8bbe\u4e3a60\u5fae\u7c73\uff0c\u5e76\u91c7\u7528\u57fa\u4e8e\u56fe\u50cf\u81ea\u52a8\u5316\u8ba1\u6570\u4eea\u4e0a\u7684\u6700\u9ad8\u53bb\u56e2\u7a0b\u5ea6\uff0c\u53ef\u4ee5\u83b7\u5f97\u51c6\u786e\u7684\u7ec6\u80de\u8ba1\u6570\u6570\u636e\u3002<\/li>\n\n\n\n
                  4. \u7ec6\u80de\u5728\u4f20\u4ee3\u8fc7\u7a0b\u4e2d\u7684\u5b58\u6d3b\u7387\uff1a<\/strong>\u80bf\u7624\u7c7b\u5668\u5b98\u7684\u7ec6\u80de\u5b58\u6d3b\u7387\u5177\u6709\u53d8\u5f02\u6027\uff0c\u5e76\u4e14\u4e0d\u540c\u4f9b\u4f53\u4e4b\u95f4\u53ef\u80fd\u5b58\u5728\u663e\u8457\u5dee\u5f02\u3002\u89e3\u79bb\u540e\uff0c\u80bf\u7624\u7c7b\u5668\u5b98\u7684\u5b58\u6d3b\u7387\uff08\u901a\u8fc7\u53f0\u76fc\u84dd\u6392\u9664\u6cd5<\/a>\uff09\u901a\u5e38\u572865-90%\u4e4b\u95f4\u3002\u867d\u7136\u8fd9\u4e9b\u5b58\u6d3b\u7387\u4f4e\u4e8e\u6c38\u751f\u5316\u7ec6\u80de\u7cfb\u901a\u5e38\u89c2\u5bdf\u5230\u7684\u6c34\u5e73\uff0c\u4f46\u5b83\u4eec\u5e94\u5728\u591a\u6b21\u4f20\u4ee3\u8fc7\u7a0b\u4e2d\u4fdd\u6301\u7a33\u5b9a\uff0c\u5e76\u4e0d\u4e00\u5b9a\u8868\u793a\u57f9\u517b\u4e2d\u7ec6\u80de\u5065\u5eb7\u72b6\u51b5\u8f83\u5dee\u3002<\/li>\n<\/ol>\n\n\n\n

                    \u89e3\u79bb\u540e\uff0c\u57f9\u517b\u7269\u5e94\u7531\u5355\u4e2a\u7ec6\u80de\u548c\u5c0f\u578b\u7ec6\u80de\u56e2\u6df7\u5408\u7ec4\u6210\uff0c\u5982\u4e0b\u56fe\u6240\u793a\u3002<\/p>\n\n\n

                    \n
                    \"Representative<\/figure>\n<\/div>\n\n\n

                    \u56fe10\uff1a\u80bf\u7624\u7c7b\u5668\u5b98\u89e3\u79bb\u540e\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff0c\u7eff\u8272\u4e3a\u5b58\u6d3b\u7ec6\u80de\uff0c\u7ea2\u8272\u4e3a\u6b7b\u4ea1\u7ec6\u80de\u3002\u5728\u8ba1\u6570\u65f6\uff0c\u5e94\u5c3d\u91cf\u7edf\u8ba1\u805a\u96c6\u4e2d\u7684\u5355\u4e2a\u7ec6\u80de\uff0c\u4ee5\u83b7\u5f97\u6700\u51c6\u786e\u7684\u7ec6\u80de\u6570\u91cf\u5b9a\u91cf\u7ed3\u679c\u3002<\/p>\n\n\n\n

                    \u764c\u75c7\u7c7b\u5668\u5b98\u57f9\u517b\u7684\u5176\u4ed6\u6ce8\u610f\u4e8b\u9879<\/strong><\/h3>\n\n\n\n
                      \n
                    1. \u60ac\u6d6e\u57f9\u517b\u671f\u95f4\u7684\u7ec6\u80de\u9ecf\u9644\uff1a<\/strong>\u67d0\u4e9b\u80bf\u7624\u7c7b\u5668\u5b98\u5929\u751f\u201c\u9ecf\u6027\u201d\u8f83\u5f3a\uff0c\u5728\u60ac\u6d6e\u57f9\u517b\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u4f1a\u9ecf\u9644\u4e8e\u975e\u7ec4\u7ec7\u57f9\u517b\u5904\u7406\u8fc7\u7684\u74f6\u58c1\u3002\u5982\u679c\u53d1\u73b0\u67d0\u4e00\u80bf\u7624\u7c7b\u5668\u5b98\u7cfb\u51fa\u73b0\u9ecf\u9644\u73b0\u8c61\uff0c\u53ef\u5728\u64ad\u79cd\u524d\uff0c\u5c06\u5b54\u677f\u6216\u74f6\u75287.5% BSA\u6eb6\u6db2\uff08\u7528\u65e0\u9499\u3001\u65e0\u9541DPBS\u7a00\u91ca\uff09\u572837\u00b0C\u7ec6\u80de\u57f9\u517b\u7bb1\u4e2d\u6d82\u898630\u5206\u949f\u3002BSA\u6eb6\u6db2\u53ef\u5728\u52a0\u5165\u80bf\u7624\u7c7b\u5668\u5b98\u60ac\u6db2\uff08\u4f7f\u7528OncoPro Tumoroid Culture Medium\uff09\u8fdb\u884c\u521d\u6b21\u64ad\u79cd\u6216\u6362\u6db2\u540e\u7acb\u5373\u5438\u51fa\uff0c\u65e0\u9700\u8fdb\u884c\u4e2d\u95f4\u6e05\u6d17\u6b65\u9aa4\u3002<\/li>\n\n\n\n
                    2. \u5305\u57cb\u57f9\u517b\u671f\u95f4\u7684\u7ec6\u80de\u9ecf\u9644\uff1a<\/strong>\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u5728BME\u6c34\u51dd\u80f6\u4e2d\u57f9\u517b\u65f6\u53ef\u80fd\u4f1a\u89c2\u5bdf\u5230\u7ec6\u80de\u9ecf\u9644\u548c\u6269\u5c55\u3002\u4e3a\u51cf\u5c11\u7ec6\u80de\u5bf9\u7ec6\u80de\u57f9\u517b\u5851\u6599\u7684\u9ecf\u9644\uff0c\u53ef\u4ee5\u4f7f\u7528\u975e\u7ec4\u7ec7\u57f9\u517b\u5904\u7406\u7684\u5b54\u677f\u548c\u57f9\u517b\u76bf\u3002\u5982\u679c\u64cd\u4f5c\u5f97\u5f53\uff0c\u6c34\u51dd\u80f6\u7a79\u9876\u5728\u521d\u59cb\u7ec6\u80de\u63a5\u79cd\u8fc7\u7a0b\u4e2d\u5012\u7f6e\u4ee5\u805a\u5408BME\u65f6\uff0c\u4f1a\u4fdd\u6301\u5728\u8fd9\u4e9b\u5bb9\u5668\u4e0a\u3002\u5982\u6709\u9700\u8981\uff0c\u6536\u96c6BME\u7a79\u9876\u4e2d\u7684\u80bf\u7624\u7c7b\u5668\u5b98\u7ec6\u80de\u540e\uff0c\u53ef\u4f7f\u7528TrypLE\u6216\u7c7b\u4f3c\u89e3\u79bb\u8bd5\u5242\u5206\u79bb\u8d34\u58c1\u7ec6\u80de\u3002<\/li>\n\n\n\n
                    3. \u8840\u6e05\u79fb\u6db2\u7ba1\u548c\u79fb\u6db2\u67aa\u5934\u9884\u6e7f\uff1a<\/strong>\u6211\u4eec\u5efa\u8bae\u5728\u5904\u7406\u80bf\u7624\u7c7b\u5668\u5b98\u524d\u9884\u5148\u6e7f\u6da6\u8840\u6e05\u79fb\u6db2\u7ba1\u548c\u79fb\u6db2\u67aa\u5934\uff0c\u4ee5\u51cf\u5c11\u7ec6\u80de\/\u80bf\u7624\u7c7b\u5668\u5b98\u5bf9\u57f9\u517b\u5851\u6599\u7684\u9ecf\u9644\u53ca\u968f\u540e\u7684\u7ec6\u80de\u635f\u5931\u3002\u5bf9\u4e8e\u5141\u8bb8\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u4e22\u5931\u90e8\u5206\u7ec6\u80de\u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u9884\u6e7f\u53ef\u80fd\u4e0d\u662f\u5fc5\u9700\u7684\u3002<\/li>\n<\/ol>\n\n\n\n

                      \u5229\u7528OncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u63a8\u52a8\u764c\u75c7\u7814\u7a76\u8fdb\u5c55<\/strong><\/strong><\/h2>\n\n\n\n

                      \u764c\u75c7\u7c7b\u5668\u5b98\u6a21\u578b\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5f3a\u5927\u7684\u5e73\u53f0\u7528\u4e8e\u7814\u7a76\u80bf\u7624\u751f\u7269\u5b66\u3002\u901a\u8fc7\u7406\u89e3\u5e76\u5229\u7528\u764c\u75c7\u7c7b\u5668\u5b98\uff0c\u7814\u7a76\u4eba\u5458\u80fd\u591f\u63a8\u52a8\u764c\u75c7\u9886\u57df\u7684\u53d1\u5c55\uff0c\u5e76\u52a9\u529b\u66f4\u6709\u6548\u6cbb\u7597\u65b9\u6cd5\u7684\u5f00\u53d1\u3002\u5b9a\u671f\u6210\u50cf\u3001\u6301\u7eed\u5582\u517b\u4ee5\u53ca\u9002\u5f53\u4f20\u4ee3\u662f\u786e\u4fdd\u8fd9\u4e9b\u4e09\u7ef4\u6a21\u578b\u5b58\u6d3b\u4e0e\u7a33\u5065\u7684\u5173\u952e\u5b9e\u8df5\u3002\u8be6\u7ec6\u534f\u8bae\u3001\u63a8\u8350\u7ec6\u80de\u5bc6\u5ea6\u53ca\u57f9\u517b\u57fa\u4f53\u79ef\u5df2\u5305\u542b\u4e8eOncoPro\u80bf\u7624\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\u7528\u6237\u6307\u5357<\/a>\u3002<\/p>\n\n\n\n

                      \u6ce8\uff1a\u90e8\u5206\u56fe\u7247\u6539\u7f16\u81eaPaul, C.D. \u7b49\u4eba bioRxiv 10.1101\/2024.06.10.598331v1 (2024)\u3002<\/p>\n\n\n\n

                      \u76f8\u5173\u5185\u5bb9<\/strong><\/strong><\/h3>\n\n\n\n

                      \u764c\u75c7\u7c7b\u5668\u5b98\u4e0e\u764c\u75c7\u7403\u4f53\u6bd4\u8f83<\/a><\/p>\n\n\n\n

                      \u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/em><\/em><\/p>\n\n\n\n

                      \"contact<\/a><\/figure>\n\n\n\n

                      <\/p>\n","protected":false},"excerpt":{"rendered":"

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