{"id":742,"date":"2026-03-09T09:13:12","date_gmt":"2026-03-09T09:13:12","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=742"},"modified":"2026-05-13T02:49:42","modified_gmt":"2026-05-13T02:49:42","slug":"real-solutions-to-improve-your-hplc-peak-resolution","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/real-solutions-to-improve-your-hplc-peak-resolution\/","title":{"rendered":"HPLC\u5cf0\u5206\u8fa8\u7387\u63d0\u5347\u7684\u5b9e\u9645\u89e3\u51b3\u65b9\u6848"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/download-solution.html?icid=cn-blog-cmd\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1024x176.jpg\" alt=\"\" class=\"wp-image-1799\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p>\u5982\u679c\u4f60\u6b63\u5728\u5bfb\u627e\u4e00\u4efd\u5e2e\u52a9\u4f60\u83b7\u5f97\u6700\u4f73\u5cf0\u5206\u79bb\u5ea6\u7684\u5feb\u901f\u89e3\u51b3\u65b9\u6848\u6e05\u5355\uff0c\u4e0d\u5fc5\u518d\u7ee7\u7eed\u641c\u7d22\u4e86\u3002\u672c\u6307\u5357\u5c06\u89e3\u91ca\u5982\u4f55\u901a\u8fc7\u5173\u6ce8\u5206\u6790\u8fc7\u7a0b\u4e2d\u7684\u6bcf\u4e00\u6b65\uff0c\u5e2e\u52a9\u4f60\u5b9e\u73b0\u66f4\u597d\u7684\u5cf0\u5206\u79bb\u5ea6\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright size-full is-resized\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/08\/HPLC-Peak-Performance-2.jpg\" alt=\"\" class=\"wp-image-15540\" style=\"width:444px;height:453px\" \/><\/figure>\n<\/div>\n\n\n<p>\u5cf0\u7684\u57fa\u7ebf\u5206\u79bb\uff08\u5373\u68c0\u6d4b\u5668\u4fe1\u53f7\u5728\u5404\u4e2a\u5cf0\u4e4b\u95f4\u56de\u5230\u57fa\u7ebf\uff09\u662f\u6bcf\u4e00\u6b21<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information\/hplc-basics.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09<\/a>\u5206\u6790\u7684\u76ee\u6807\u3002\u8fd9\u662f\u56e0\u4e3a\u5728\u5404\u5bf9\u5cf0\u4e4b\u95f4\u6709\u8db3\u591f\u7684\u5206\u79bb\u5ea6\uff0c\u53ef\u4ee5\u786e\u4fdd\u4f60\u51c6\u786e\u8bc6\u522b\u5e76\u5feb\u901f\u5b9a\u91cf\u6837\u54c1\u4e2d\u7684\u6240\u6709\u5206\u6790\u7269\u3002(HPLC) \u5206\u6790\u3002\u8fd9\u662f\u56e0\u4e3a\u5728\u5404\u5bf9\u5cf0\u4e4b\u95f4\u6709\u8db3\u591f\u7684\u5206\u79bb\u5ea6\uff0c\u53ef\u4ee5\u786e\u4fdd\u4f60\u51c6\u786e\u8bc6\u522b\u5e76\u5feb\u901f\u5b9a\u91cf\u6837\u54c1\u4e2d\u7684\u6240\u6709\u5206\u6790\u7269\u3002<\/p>\n\n\n\n<p>\u975e\u57fa\u7ebf\u5206\u79bb<br>\u2014\u2014\u6216\u79f0\u4e3a\u5cf0\u5171\u6d17\u8131\u2014\u2014\u901a\u5e38\u7531\u5cf0\u62d6\u5c3e\u589e\u52a0\u3001\u9009\u62e9\u6027\u53d8\u5316\u548c\u7075\u654f\u5ea6\u5dee\u5f15\u8d77\u3002\u8fd9\u79cd\u5c40\u9650\u6027\u4e0d\u4ec5\u5f71\u54cd\u4f60\u5feb\u901f\u5ba1\u67e5\u6570\u636e\u7684\u80fd\u529b\uff0c\u8fd8\u4f1a\u7ed9\u4f60\u7684\u5206\u6790\u5e26\u6765\u53d8\u5f02\u3002\u56e0\u6b64\uff0c\u4f60\u53ef\u4ee5\u7406\u89e3\u4e3a\u4ec0\u4e48\u4f18\u5316\u5206\u6790\u7684\u5206\u8fa8\u7387\u5bf9\u4e8e\u63d0\u5347\u6570\u636e\u8d28\u91cf\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n<p>\u5f04\u6e05\u695a\u5982\u4f55\u4f18\u5316\u4f60\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information\/hplc-method-development-steps.html\">HPLC\u5b9e\u9a8c<\/a>\u7684\u5206\u8fa8\u7387\u53ef\u80fd\u662f\u4e00\u9879\u8270\u5de8\u4efb\u52a1\u3002\u4eba\u4eec\u603b\u4f1a\u9996\u5148\u60f3\u5230\uff1a\u201c\u6211\u5230\u5e95\u8be5\u4ece\u54ea\u91cc\u5f00\u59cb\uff1f\u201d\u8fd9\u6b63\u662f\u672c\u6307\u5357\u80fd\u591f\u5e2e\u4e0a\u5fd9\u7684\u5730\u65b9\u3002\u53ef\u80fd\u662f\u4e00\u9879\u4ee4\u4eba\u751f\u754f\u7684\u4efb\u52a1\u3002\u603b\u4f1a\u6709\u4e00\u79cd\u201c\u6211\u5230\u5e95\u8be5\u4ece\u54ea\u91cc\u5f00\u59cb\uff1f\u201d\u7684\u611f\u89c9\u3002\u8fd9\u6b63\u662f\u672c\u6307\u5357\u53ef\u4ee5\u5e2e\u5230\u4f60\u7684\u5730\u65b9\u3002<\/p>\n\n\n\n<p>\u63d0\u9ad8\u5206\u8fa8\u7387\u7684\u4e00\u4e9b\u65b9\u6cd5\u5305\u62ec\u91c7\u7528\u65b0\u7684\u8272\u8c31\u67f1\u5316\u5b66\u6280\u672f\u4ee5\u53ca\u4f7f\u7528\u73b0\u4ee3\u4eea\u5668\u548c\u8f6f\u4ef6\u6280\u672f\uff0c\u4f46\u5982\u679c\u60a8\u65e0\u6cd5\u5347\u7ea7\u73b0\u6709\u6280\u672f\uff0c\u4ecd\u7136\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u5f53\u524d\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u82e5\u5e72\u56e0\u7d20\u6765\u6539\u5584\u5cf0\u5206\u8fa8\u7387\u3002<\/p>\n\n\n\n<p>\u8bf7\u8bb0\u4f4f\uff1a\u8981\u91c7\u7528\u7cfb\u7edf\u5316\u7684\u65b9\u6cd5\u2014\u2014\u6bcf\u6b21\u53ea\u6539\u53d8\u4e00\u4e2a\u53c2\u6570\u3001\u5176\u4f59\u4fdd\u6301\u4e0d\u53d8\u2014\u2014\u4ee5\u5224\u65ad\u6bcf\u4e00\u6b65\u8c03\u6574\u7684\u771f\u5b9e\u6548\u679c\u3002<\/p>\n\n\n\n<p>\u4ece\u6837\u54c1\u5236\u5907\u5230\u6570\u636e\u91c7\u96c6\uff0c\u4e0b\u9762\u662f\u4e00\u4efd\u53ef\u7528\u4e8e\u201c\u9010\u9879\u6392\u67e5\u4e0e\u8c03\u6574\u201d\u7684\u8be6\u7ec6\u6e05\u5355\uff0c\u5e2e\u52a9\u4f60\u6700\u5927\u5316\u5cf0\u5206\u8fa8\u7387\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-\u6837\u54c1\u4e0e\u7cfb\u7edf\u51c6\u5907\"><strong><strong>1. \u6837\u54c1\u4e0e\u7cfb\u7edf\u51c6\u5907<\/strong><\/strong><\/h3>\n\n\n\n<p><strong>\u25a1 <strong>\u6837\u54c1\u5236\u5907<\/strong><\/strong><\/p>\n\n\n\n<p>\u6bcf\u4e00\u6b21HPLC\u5206\u6790\u7684\u7b2c\u4e00\u6b65\u90fd\u662f<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-consumables.html\">\u6837\u54c1\u5236\u5907<\/a>\u3002\u6839\u636e\u60a8\u7684\u5e94\u7528\u548c\u7cfb\u7edf\u9700\u6c42\u8fdb\u884c\u9002\u5f53\u7684\u8fc7\u6ee4\u6216\u63d0\u53d6\uff0c\u53bb\u9664\u9897\u7c92\u7269\u548c\u6742\u8d28\uff0c\u5c06\u6709\u52a9\u4e8e\u63d0\u5347\u5206\u8fa8\u7387\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u6837\u54c1\u5bb9\u5668<\/strong><\/strong><\/p>\n\n\n\n<p>\u5bf9\u4e8e\u60a8\u7684\u5149\u654f\u5206\u6790\u7269\uff0c\u901a\u8fc7\u9009\u62e9\u5408\u9002\u7684\u9632\u5149\u74f6\u53ef\u4ee5\u9632\u6b62\u5206\u6790\u7269\u964d\u89e3\u3002\u758f\u6c34\u6027\/\u4eb2\u6c34\u6027\u5206\u6790\u7269\u53ef\u80fd\u9700\u8981\u60a8\u9009\u62e9\u80fd\u591f\u9632\u6b62\u5206\u6790\u7269\u4e0e\u5bb9\u5668\u8868\u9762\u53d1\u751f\u4e0d\u5fc5\u8981\u7ed3\u5408\u7684\u5bb9\u5668\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u6d41\u52a8\u76f8\u7ec4\u6210<\/strong><\/strong><\/p>\n\n\n\n<p>\u60a8\u7684\u6d41\u52a8\u76f8\u7ec4\u6210\u81f3\u5173\u91cd\u8981\uff0c\u5e76\u4e14\u4f1a\u5bf9\u5206\u6790\u7269\u4fdd\u7559\u548c\u9009\u62e9\u6027\u4ea7\u751f\u663e\u8457\u5f71\u54cd\u3002\u8bf8\u5982\u6c34\/\u6709\u673a\u6eb6\u5242\u6bd4\u4f8b\u3001\u6d41\u52a8\u76f8pH\u503c\u4ee5\u53ca\u7f13\u51b2\u6db2\u79bb\u5b50\u5f3a\u5ea6\u7b49\u56e0\u7d20\u90fd\u4f1a\u5f71\u54cd\u5206\u8fa8\u7387\u3002\u6eb6\u5242\u7ec4\u6210\u751a\u81f3\u53ef\u4ee5\u5b8c\u5168\u6539\u53d8\u60a8\u7684\u5206\u79bb\u79d1\u5b66\u3002\u60a8\u53ef\u4ee5\u53c2\u8003<a href=\"https:\/\/www.chromatographyonline.com\/view\/methods-changing-peak-resolution-hplc-advantages-and-limitations\">HPLC\u4e2d\u6539\u53d8\u5cf0\u5206\u8fa8\u7387\u7684\u65b9\u6cd5\uff1a\u4f18\u70b9\u4e0e\u5c40\u9650\u6027<\/a>\u4ee5\u83b7\u5f97\u66f4\u6df1\u5165\u7684\u7406\u8bba\u5206\u6790\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/08\/Mobile-phase-composition-on-peak-resolution-in-HPLC.png\" alt=\"\" class=\"wp-image-15542\" style=\"width:753px;height:463px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/Reference-Materials\/WS-72221-HPLC-Tips-Tricks-HPLCWinterWebinar2016-WS72221-EN.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u5728\u8fd0\u884c\u76f8\u540c\u5206\u6790\u7269\u65f6\uff0c\u6d41\u52a8\u76f8pH\u503c\u548c\u7f13\u51b2\u6db2\u6d53\u5ea6\u7684\u5dee\u5f02<\/a><\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u8272\u8c31\u67f1\u9009\u62e9<\/strong><\/strong><\/p>\n\n\n\n<p>\u4e0e\u6d41\u52a8\u76f8\u8003\u8651\u540c\u6837\u91cd\u8981\u7684\u662f\u60a8\u7684<a href=\"https:\/\/www.thermofisher.com\/order\/lc-column-guide\/#!\/\">\u8272\u8c31\u67f1\u56fa\u5b9a\u76f8<\/a>\u5728\u4f18\u5316\u5206\u8fa8\u7387\u65f6\uff0c\u8003\u8651\u8fd9\u4e9b\u56e0\u7d20\u975e\u5e38\u91cd\u8981\u3002\u53ef\u4ee5\u8003\u8651\u4f7f\u7528\u66f4\u5c0f\u7684\u9897\u7c92\u5c3a\u5bf8\u548c\u5b9e\u5fc3\u6838\u9897\u7c92\uff0c\u8fd9\u4e9b\u90fd\u80fd\u901a\u8fc7\u63d0\u9ad8\u6548\u7387\u8fdb\u4e00\u6b65\u63d0\u5347\u5206\u8fa8\u7387\u3002\u8f83\u957f\u7684\u8272\u8c31\u67f1\u901a\u5e38\u610f\u5473\u7740\u66f4\u5c0f\u7684\u9897\u7c92\u548c\u66f4\u5927\u7684\u5b54\u5f84\u3002\u8fd9\u4f1a\u6539\u5584\u5cf0\u503c\u5206\u8fa8\u7387\uff0c\u4f46\u4e5f\u4f1a\u589e\u52a0\u80cc\u538b\u548c\u5206\u6790\u65f6\u95f4\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-\u6cf5\"><strong>2. \u6cf5<\/strong><\/h3>\n\n\n\n<p>\u25a1 <strong><strong>\u6d41\u901f<\/strong><\/strong><\/p>\n\n\n\n<p>\u5f53\u4f60\u88c5\u597d\u6837\u54c1\u4e0e\u6d41\u52a8\u76f8\u5e76\u5b89\u88c5\u8272\u8c31\u67f1\u540e\uff0c\u542f\u52a8\u7cfb\u7edf\u65f6\u6700\u5148\u5f71\u54cd\u5206\u8fa8\u7387\u7684\u53c2\u6570\u4e4b\u4e00\u5c31\u662f\u6d41\u901f\u3002\u9700\u8981\u627e\u5230\u4e00\u4e2a\u5728\u4fdd\u8bc1\u603b\u4f53\u8fd0\u884c\u65f6\u95f4\u7684\u540c\u65f6\uff0c\u4f7f\u5cf0\u6548\u7387\u6700\u5927\u5316\u7684\u6700\u4f73\u6d41\u901f\u3002<\/p>\n\n\n\n<p>\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u964d\u4f4e\u6d41\u901f\u4f1a\u964d\u4f4e\u67f1\u51fa\u53e3\u5904\u7684\u4fdd\u7559\u56e0\u5b50\uff0c\u4f7f\u5cf0\u66f4\u7a84\u5e76\u5e26\u6765\u66f4\u597d\u7684\u54cd\u5e94\uff1b\u63d0\u9ad8\u6d41\u901f\u53ef\u80fd\u5bfc\u81f4\u5cf0\u53d8\u5bbd\u3001\u5206\u8fa8\u7387\u4e0b\u964d\uff0c\u4f46\u53ef\u7f29\u77ed\u8fd0\u884c\u65f6\u95f4\u3002<\/p>\n\n\n\n<p>\u91c7\u7528\u66f4\u5c0f\u9897\u7c92\u6216\u6838\u58f3\u9897\u7c92\u586b\u5145\u7684\u8272\u8c31\u67f1\uff0c\u53ef\u5728\u66f4\u9ad8\u6d41\u901f\u4e0b\u66f4\u597d\u5730\u7ef4\u6301\u8f83\u9ad8\u5cf0\u5206\u8fa8\u7387\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-\u81ea\u52a8\u8fdb\u6837\u5668\"><strong>3. \u81ea\u52a8\u8fdb\u6837\u5668<\/strong><\/h3>\n\n\n\n<p>\u6b64\u65f6\uff0c\u4f60\u7684\u6cf5\u5df2\u7ecf\u5f00\u542f\uff0c\u6eb6\u5242\u6b63\u5728\u7cfb\u7edf\u4e2d\u6d41\u52a8\u3002\u4e00\u65e6\u8fbe\u5230\u5e73\u8861\uff08\u5373\u6574\u4e2a\u6d41\u8def\u90fd\u88ab\u586b\u6ee1\uff09\uff0c\u5c31\u5230\u4e86\u6700\u6709\u8da3\u7684\u4e00\u6b65\u2014\u2014\u6837\u54c1\u8fdb\u6837\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u8fdb\u6837\u4f53\u79ef<\/strong><\/strong><\/p>\n\n\n\n<p>\u5f71\u54cd\u5206\u8fa8\u7387\u7684\u8fdb\u6837\u4f53\u79ef\u53c2\u6570\u5305\u62ec\u6837\u54c1\u6d53\u5ea6\u3001\u8272\u8c31\u67f1\u5bb9\u91cf\u4ee5\u53ca\u68c0\u6d4b\u5668\u7075\u654f\u5ea6\u3002\u5728\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u6ce8\u5165\u5230\u8272\u8c31\u67f1\u4e2d\u7684\u6837\u54c1\u8d8a\u591a\uff0c\u8d8a\u5bb9\u6613\u51fa\u73b0\u524d\u6cbf\u5cf0\u73b0\u8c61\u3002\u4fdd\u7559\u65f6\u95f4\u53ef\u80fd\u7f29\u77ed\uff0c\u5e76\u5bf9\u8272\u8c31\u67f1\u6548\u7387\u53ca\u5206\u8fa8\u7387\u4ea7\u751f\u8d1f\u9762\u5f71\u54cd\u3002\u8fd9\u79cd\u73b0\u8c61\u88ab\u79f0\u4e3a\u8d28\u91cf\u8d85\u8f7d\u3002<\/p>\n\n\n\n<p>\u8fc7\u91cf\u52a0\u8f7d\u6837\u54c1\u5230\u8272\u8c31\u67f1\u4e0d\u4ec5\u4f1a\u4f7f\u68c0\u6d4b\u5668\u8fbe\u5230\u6700\u5927\u9650\u5ea6\uff0c\u8fd8\u4f1a\u5bfc\u81f4\u5176\u4ed6\u975e\u7406\u60f3\u60c5\u51b5\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u5982\u679c\u6837\u54c1\u6d53\u5ea6\u4e3a1\u00b5g\/\u00b5l\uff0c\u5efa\u8bae\u6ce8\u5c04\u91cf\u4e3a\u8272\u8c31\u67f1\u603b\u4f53\u79ef\u76841-2%\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-4-\u67f1\u6e29-\u7bb1\"><strong>4. \u67f1\u6e29<\/strong>\u7bb1<\/h3>\n\n\n\n<p>\u4ece\u6cf5\u5230\u81ea\u52a8\u8fdb\u6837\u5668\uff0c\u4f18\u5316\u6d41\u7a0b\u7684\u4e0b\u4e00\u7ad9\u5c31\u662f\u8272\u8c31\u67f1\u4ed3\u3002\u867d\u7136\u6211\u4eec\u5df2\u7ecf\u8003\u8651\u4e86\u8272\u8c31\u67f1\u5316\u5b66\u6027\u8d28\uff0c\u4f60\u8fd8\u9700\u8981\u786e\u4fdd\u8272\u8c31\u67f1\u7684\u5c3a\u5bf8\u3001\u957f\u5ea6\u548c\u6761\u4ef6\u80fd\u591f\u5982\u9884\u671f\u54cd\u5e94\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u8272\u8c31\u67f1\u6e29\u5ea6<\/strong><\/strong><\/p>\n\n\n\n<p>\u8272\u8c31\u67f1\u4ed3\u7684\u6e29\u5ea6\u5728\u5206\u79bb\u6548\u7387\u548c\u9009\u62e9\u6027\u65b9\u9762\u8d77\u7740\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n\n\n\n<p>\u8f83\u9ad8\u7684\u6e29\u5ea6\u53ef\u4ee5\u5b9e\u73b0\u66f4\u5feb\u7684\u6d41\u901f\u548c\u66f4\u8fc5\u901f\u7684\u5206\u6790\uff0c\u4f46\u53e6\u4e00\u65b9\u9762\uff0c\u4e5f\u53ef\u80fd\u5bfc\u81f4\u6837\u54c1\u964d\u89e3\u548c\u5206\u8fa8\u7387\u964d\u4f4e\u3002\u8f83\u4f4e\u7684\u8272\u8c31\u67f1\u6e29\u5ea6\u5219\u80fd\u63d0\u9ad8\u4fdd\u7559\u65f6\u95f4\uff0c\u6539\u5584\u5cf0\u5206\u8fa8\u7387\uff0c\u4f46\u5206\u6790\u901f\u5ea6\u4f1a\u53d8\u6162\u3002<\/p>\n\n\n\n<p>\u786e\u4fdd\u4f60\u4e86\u89e3\u6837\u54c1\u3001\u6eb6\u5242\u3001\u8272\u8c31\u67f1\u53ca\u4eea\u5668\u8bbe\u5907\u7684\u6e29\u5ea6\u9650\u5236\uff0c\u5e76\u5728\u8fd9\u4e9b\u6307\u5bfc\u8303\u56f4\u5185\u9010\u6b65\u8c03\u6574\u53c2\u6570\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/08\/Temperature-effects-on-peak-resolution-in-HPLC.png\" alt=\"\" class=\"wp-image-15558\" style=\"width:830px;height:370px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/Reference-Materials\/WP-71499-LC-Temperature-Column-Thermostatting-WP71499-EN.pdf\">\u540c\u4e00\u6a21\u578b\u6837\u54c1\u5728\u4e0d\u540c\u6e29\u5ea6\u4e0b\u5206\u79bb\u6240\u5f97\u53e0\u52a0\u8272\u8c31\u56fe<\/a>\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u7cfb\u7edf\u80cc\u538b<\/strong><\/strong><\/p>\n\n\n\n<p>\u5f53\u6d41\u52a8\u76f8\u901a\u8fc7\u8272\u8c31\u67f1\/HPLC\u7cfb\u7edf\u65f6\uff0c\u4f1a\u4ea7\u751f\u4e00\u5b9a\u538b\u529b\uff0c\u8fd9\u901a\u5e38\u88ab\u79f0\u4e3a\u7cfb\u7edf\u80cc\u538b\u3002\u8bb0\u5f55\u7cfb\u7edf\u80cc\u538b\u662f\u4f18\u5316\u8fc7\u7a0b\u4e2d\u7684\u6709\u7528\u5de5\u5177\uff0c\u5c24\u5176\u662f\u5728\u4f60\u7684\u8272\u8c31\u67f1\u548c\u4eea\u5668\u9010\u6e10\u8001\u5316\u65f6\u3002\u80cc\u538b\u589e\u52a0\u6216\u8fc7\u9ad8\u53ef\u80fd\u610f\u5473\u7740\u5835\u585e\u6216\u8272\u8c31\u67f1\u635f\u574f\u3002\u9002\u5f53\u7ef4\u62a4\u3001\u6e05\u6d17\u6216\u66f4\u6362\u8272\u8c31\u67f1\u53ef\u4ee5\u8ba9\u4f60\u7684\u7cfb\u7edf\u6062\u590d\u6700\u4f73\u72b6\u6001\u3002\u59cb\u7ec8\u53c2\u8003\u8272\u8c31\u67f1\u5236\u9020\u5546\u63a8\u8350\u7684\u64cd\u4f5c\u7a0b\u5e8f\u548c\u6307\u5357\uff0c\u4ee5\u83b7\u5f97\u6700\u4f73\u7ef4\u62a4\u4e0e\u6e05\u6d17\u65b9\u6cd5\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-5-\u68c0\u6d4b\u5668\"><strong>5. \u68c0\u6d4b\u5668<\/strong><\/h3>\n\n\n\n<p>\u68c0\u6d4b\u5668\u662f\u60a8\u7684\u6837\u54c1\u5206\u6790\u8fc7\u7a0b\u4e2d\u7684\u6700\u540e\u4e00\u7ad9\u3002\u5728\u5f00\u59cb\u4f18\u5316\u68c0\u6d4b\u5668\u8bbe\u7f6e\u4e4b\u524d\uff0c\u8bf7\u5148\u8003\u8651\u60a8\u662f\u5426\u5df2\u7ecf\u4e3a\u8be5\u4efb\u52a1\u9009\u62e9\u4e86\u6b63\u786e\u7684\u68c0\u6d4b\u5668\u3002\u53ef\u53d8\u6ce2\u957f\u3001\u4e8c\u6781\u7ba1\u9635\u5217\u3001<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information\/hplc-system-components\/how-hplc-detectors-work\/charged-aerosol-detection.html\">\u5e26\u7535\u6c14\u6eb6\u80f6<\/a>\u3001\u6298\u5149\u6307\u6570\u3001\u8367\u5149\u3001\u5355\u56db\u6781\u6746\u8d28\u8c31\u4eea\u2026\u2026\u79cd\u7c7b\u7e41\u591a\u3002\u8bf7\u786e\u4fdd\u60a8\u6839\u636e\u5206\u6790\u7269\u6027\u8d28\u3001\u7075\u654f\u5ea6\u3001\u68c0\u6d4b\u8303\u56f4\u7b49\u56e0\u7d20\uff0c\u9009\u62e9\u4e86<a href=\"https:\/\/www.thermofisher.com\/blog\/analyteguru\/which-hplc-detector-fits-your-analytes-best\/\">\u6240\u9700\u7684\u68c0\u6d4b\u5668<\/a>\u3002\u57fa\u4e8e\u5206\u6790\u7269\u6027\u8d28\u3001\u7075\u654f\u5ea6\u3001\u8303\u56f4\u7b49\u56e0\u7d20\u3002<\/p>\n\n\n\n<p>\u25a1 <strong><strong>\u68c0\u6d4b\u5668\u8bbe\u7f6e<\/strong><\/strong><\/p>\n\n\n\n<p>\u4e00\u65e6\u786e\u5b9a\u4e86\u6700\u9002\u5408\u60a8\u5206\u6790\u7684\u68c0\u6d4b\u5668\uff0c\u5c31\u9700\u8981\u786e\u4fdd\u5206\u8fa8\u7387\u8fbe\u5230\u8981\u6c42\u3002\u60a8\u5fc5\u987b\u8003\u8651\u7684\u4e3b\u8981\u53c2\u6570\u6709\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6ce2\u957f\uff1a<\/strong>\u5728\u4f7f\u7528\u7d2b\u5916-\u53ef\u89c1\uff08UV-Vis\uff09\u68c0\u6d4b\u5668\u65f6\uff0c\u6ce2\u957f\u5e94\u4f5c\u4e3a\u9996\u8981\u4f18\u5316\u53c2\u6570\u3002\u901a\u8fc7\u8bc4\u4f30\u76ee\u6807\u5206\u6790\u7269\u7684\u5438\u6536\u5149\u8c31\uff0c\u53ef\u4ee5\u627e\u5230\u4ea7\u751f\u6700\u9ad8\u5438\u6536\u7684\u6700\u4f73\u6ce2\u957f\u3002\u8fd9\u4e00\u6280\u672f\u80fd\u591f\u6700\u5927\u7a0b\u5ea6\u51cf\u5c11\u5e72\u6270\uff0c\u5e76\u63d0\u4f9b\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u3002<\/li>\n\n\n\n<li><strong>\u54cd\u5e94\u65f6\u95f4\uff1a<\/strong>\u4e00\u822c\u7684\u7ecf\u9a8c\u6cd5\u5219\u662f\uff0c\u4f60\u5e94\u8be5\u5c06\u54cd\u5e94\u65f6\u95f4\u8bbe\u7f6e\u4e3a\u4f60\u5173\u6ce8\u7684\u6700\u7a84\u5cf0\u5728\u534a\u9ad8\u5bbd\u5ea6\u7684\u4e09\u5206\u4e4b\u4e00\u3002\uff08\u8fd9\u53e5\u8bdd\u53ef\u80fd\u9700\u8981\u4f60\u591a\u8bfb\u51e0\u904d\uff0c\u6216\u8005\u62ff\u51fa\u7eb8\u548c\u7b14\u6765\u8ba1\u7b97\u4e00\u4e0b\u3002\uff09<\/li>\n\n\n\n<li><strong>\u6570\u636e\u91c7\u96c6\uff1a<\/strong>\u6570\u636e\u70b9\u592a\u5c11<a href=\"https:\/\/www.crawfordscientific.com\/chromatography-blog\/post\/peak-purity-analysis\">\u4f1a\u5f71\u54cd\u4f60\u7684\u5cf0\u5f62<\/a>\u3001\u91cd\u73b0\u6027\u4ee5\u53ca\u9762\u79ef\u7cbe\u5ea6\u3002\u6bcf\u4e2a\u8272\u8c31\u5cf0\u81f3\u5c11\u9700\u898120\u4e2a\u6570\u636e\u70b9\u2014\u2014\u4f46\u7406\u60f3\u60c5\u51b5\u4e0b\u5e94\u4e3a30-40\u4e2a\u2014\u2014\u624d\u80fd\u83b7\u5f97\u6700\u4f73\u7684\u5cf0\u5206\u8fa8\u7387\u3002<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright size-large is-resized\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/08\/HPLC-peak-resolution-solution-blog-410x1024.jpg\" alt=\"\" class=\"wp-image-15562\" style=\"width:318px;height:794px\" \/><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u800c\u4e14\u522b\u5fd8\u4e86\u7ef4\u62a4\u4f60\u7684hplc\u7cfb\u7edf\"><strong><strong>\u800c\u4e14\u522b\u5fd8\u4e86\u7ef4\u62a4\u4f60\u7684HPLC\u7cfb\u7edf\uff01<\/strong><\/strong><\/h3>\n\n\n\n<p>\u4f18\u5316\u4eea\u5668\u6216<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/vanquish-method-development-hplc-and-uhplc-systems.html\">\u65b9\u6cd5<\/a>\u7684\u5206\u8fa8\u7387\u53ef\u80fd\u662f\u4e00\u4e2a\u8017\u65f6\u7684\u8fc7\u7a0b\uff0c\u4f46\u8fd9\u662f\u4efb\u4f55\u5206\u6790\u4e2d\u91cd\u8981\u7684\u4e00\u90e8\u5206\uff0c\u5e76\u4e14\u4f1a\u5e2e\u52a9\u4f60\u66f4\u52a0\u719f\u6089\u4f60\u7684\u4eea\u5668\u3002\u4e00\u65e6\u4f60\u8fbe\u5230\u4e86\u7406\u60f3\u7684\u5206\u8fa8\u7387\uff0c\u8bf7\u8bb0\u4f4f\uff0c\u4f60\u7684\u5de5\u4f5c\u8fd8\u6ca1\u6709\u7ed3\u675f\u3002\u53ef\u80fd\u662f\u4e00\u4e2a\u8017\u65f6\u7684\u8fc7\u7a0b\uff0c\u4f46\u5b83\u662f\u4efb\u4f55\u5206\u6790\u4e2d\u91cd\u8981\u7684\u4e00\u90e8\u5206\uff0c\u5e76\u4e14\u4f1a\u5e2e\u52a9\u4f60\u66f4\u52a0\u719f\u6089\u4f60\u7684\u4eea\u5668\u3002\u4e00\u65e6\u4f60\u627e\u5230\u4e86\u89e3\u51b3\u65b9\u6848\uff0c\u8bf7\u8bb0\u4f4f\uff0c\u4f60\u7684\u5de5\u4f5c\u8fd8\u6ca1\u6709\u5b8c\u6210\u3002<\/p>\n\n\n\n<p>\u60a8\u8fd8\u9700\u8981\u786e\u4fdd\u60a8\u7684\u4eea\u5668\u4e0d\u4ec5\u8fd0\u884c\u5f97\u6700\u4f73\uff0c\u800c\u4e14\u4fdd\u6301\u4e00\u81f4\u3002\u8fd9\u610f\u5473\u7740\u4e0d\u8981\u57288\/24\u5c0f\u65f6\u7684\u4f7f\u7528\u65f6\u95f4\u91cc\u7528\u7a7a\u8c03\u731b\u5439\u7cfb\u7edf\uff0c\u5e76\u4e14\u8981\u8fc5\u901f\u53d1\u73b0\u5e76\u89e3\u51b3\u6cc4\u6f0f\u3001\u5835\u585e\u6216\u5176\u4ed6\u95ee\u9898\u3002<\/p>\n\n\n\n<p>\u6ce8\u610f<a 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