{"id":885,"date":"2026-03-12T00:59:22","date_gmt":"2026-03-12T00:59:22","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=885"},"modified":"2026-03-12T00:59:23","modified_gmt":"2026-03-12T00:59:23","slug":"pcr-primer-design-tips","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/pcr-primer-design-tips\/","title":{"rendered":"PCR\u5f15\u7269\u8bbe\u8ba1\u6280\u5de7"},"content":{"rendered":"\n<p>\u8bbe\u8ba1\u5be1\u6838\u82f7\u9178\u5e76\u786e\u4fdd\u60a8\u62e5\u6709\u6b63\u786e\u7684\u53c2\u6570<a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/what-is-an-oligo\/?icid=BID_SBOLG_PJT3762_WhatIsOligohttp:\/\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u5be1\u6838\u82f7\u9178<\/a>\u662f\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u91cd\u8981\u6b65\u9aa4\uff0c\u5c24\u5176\u662f\u5728<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/oligonucleotides-primers-probes-genes\/applied-biosystems-custom-primers-probes.html?icid=BID_SBOLG_PJT3762_PCRprimerDesign\" target=\"_blank\" rel=\"noopener noreferrer\">PCR\u5f15\u7269\u8bbe\u8ba1<\/a>\u4e2d\u3002\u4e3a\u4e86\u5b9e\u73b0\u6210\u529f\u7684DNA\u6269\u589e\uff0c\u9009\u62e9\u5408\u9002\u7684\u5f15\u7269\u975e\u5e38\u91cd\u8981\u3002\n\n<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/09\/pcr-primer.jpg\" alt=\"\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u6837\u7684\u5f15\u7269\u624d\u7b97\u597d\u5f15\u7269\"><strong>\u4ec0\u4e48\u6837\u7684\u5f15\u7269\u624d\u7b97\u597d\u5f15\u7269\uff1f<\/strong><\/h2>\n\n\n\n<p>\n\u4ee5\u4e0b\u662f\u8bbe\u8ba1\u60a8\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/oligonucleotides-primers-probes-genes\/applied-biosystems-5-prime-labeled-primers.html?icid=BID_SBOLG_PJT3762_PCRprimers\" target=\"_blank\" rel=\"noopener noreferrer\">PCR\u5f15\u7269\u7684\u4e00\u4e9b\u6307\u5bfc\u539f\u5219<\/a>\uff1a\n<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>GC\u542b\u91cf\u5e94\u63a7\u5236\u572840%\u523060%\u4e4b\u95f4\uff0c\u5e76\u4e14\u5f15\u72693\u2019\u7aef\u4ee5G\u6216C\u7ed3\u5c3e\uff0c\u4ee5\u4fc3\u8fdb\u7ed3\u5408\u3002\u8fd9\u88ab\u79f0\u4e3aGC\u5939\u3002G\u548cC\u78b1\u57fa\u5177\u6709\u66f4\u5f3a\u7684\u6c22\u952e\uff0c\u6709\u52a9\u4e8e\u63d0\u9ad8\u5f15\u7269\u7684\u7a33\u5b9a\u6027\u3002\u6ce8\u610f\u4e0d\u8981\u6709\u8fc7\u591a\u91cd\u590d\u7684G\u6216C\u78b1\u57fa\uff0c\u5426\u5219\u53ef\u80fd\u5bfc\u81f4\u5f15\u7269\u4e8c\u805a\u4f53\u5f62\u6210\u3002<\/li>\n\n\n\n<li>PCR\u5f15\u7269\u7684\u7406\u60f3\u957f\u5ea6\u901a\u5e38\u4e3a18-30\u4e2a\u78b1\u57fa\u3002\u7279\u5f02\u6027\u901a\u5e38\u53d6\u51b3\u4e8e\u957f\u5ea6\u548c\u9000\u706b\u6e29\u5ea6\u3002\u5f15\u7269\u8d8a\u77ed\uff0c\u7ed3\u5408\u6216\u9000\u706b\u5230\u76ee\u6807\u5e8f\u5217\u7684\u6548\u7387\u5c31\u8d8a\u9ad8\u3002<\/li>\n\n\n\n<li>\u5c3d\u91cf\u4f7f\u5f15\u7269\u7684\u7194\u89e3\u6e29\u5ea6\uff08Tm\uff09\u4fdd\u6301\u572865\u00b0C\u523075\u00b0C\u4e4b\u95f4\uff0c\u5e76\u4e14\u4e24\u8005\u4e4b\u95f4\u76f8\u5dee\u4e0d\u8d85\u8fc75\u00b0C\u3002\u7531\u4e8eTm\u53d7\u957f\u5ea6\u5f71\u54cd\uff0c\u56e0\u6b64\u5efa\u8bae\u5c06\u5f15\u7269\u8bbe\u8ba1\u5f97\u8f83\u77ed\u3002\u78b1\u57fa\u7c7b\u578b\u4e5f\u4f1a\u5f71\u54cdTm\uff0cG\u548cC\u4f1a\u5bfc\u81f4\u66f4\u9ad8\u7684\u7194\u89e3\u6e29\u5ea6\uff0c\u800cA\u548cT\u5219\u8f83\u4f4e\u3002\u5982\u679c\u60a8\u7684\u5f15\u7269Tm\u5f88\u4f4e\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u9009\u62e9\u542b\u6709\u66f4\u591aGC\u7684\u5e8f\u5217\uff0c\u6216\u8005\u9002\u5f53\u5ef6\u957f\u5f15\u7269\u957f\u5ea6\u3002<\/li>\n\n\n\n<li>\u901a\u5e38\uff0c\u5728\u5f15\u7269\u4e2d\u9650\u5236\u6027\u9176\u4f4d\u70b9\u76845&#8217;\u7aef\u6dfb\u52a03\u52304\u4e2a\u6838\u82f7\u9178\uff0c\u4ee5\u4fbf\u5b9e\u73b0\u9ad8\u6548\u5207\u5272\u3002<\/li>\n\n\n\n<li>\u5c3d\u91cf\u907f\u514d\u51fa\u73b0\u4e8c\u7ea7\u7ed3\u6784\u533a\u57df\uff0c\u5e76\u786e\u4fddGC\u4e30\u5bcc\u533a\u548cAT\u4e30\u5bcc\u533a\u5206\u5e03\u5747\u8861\u3002<\/li>\n\n\n\n<li>\u5c3d\u91cf\u907f\u514d\u8fde\u7eed\u51fa\u73b04\u4e2a\u6216\u4ee5\u4e0a\u540c\u4e00\u79cd\u78b1\u57fa\uff0c\u6216\u4e8c\u6838\u82f7\u9178\u91cd\u590d\uff08\u4f8b\u5982ACCCC\u6216ATATATAT\uff09\u3002<\/li>\n\n\n\n<li>\u907f\u514d\u5f15\u7269\u5185\u90e8\u540c\u6e90\u6027\uff08\u5373\u5f15\u7269\u5185\u6709\u8d85\u8fc73\u4e2a\u4e92\u8865\u78b1\u57fa\uff09\u6216\u5f15\u7269\u95f4\u540c\u6e90\u6027\uff08\u6b63\u5411\u4e0e\u53cd\u5411\u5f15\u7269\u4e4b\u95f4\u5b58\u5728\u4e92\u8865\u5e8f\u5217\uff09\u3002\u8fd9\u4e9b\u60c5\u51b5\u53ef\u80fd\u5bfc\u81f4\u81ea\u4e8c\u805a\u4f53\u6216\u5f15\u7269\u4e8c\u805a\u4f53\u5f62\u6210\uff0c\u800c\u4e0d\u662f\u4e0e\u76ee\u6807DNA\u5e8f\u5217\u9000\u706b\u3002<\/li>\n\n\n\n<li>\u5982\u679c\u60a8\u5c06\u5f15\u7269\u7528\u4e8e\u514b\u9686\uff0c\u6211\u4eec\u5efa\u8bae\u81f3\u5c11\u91c7\u7528\u67f1\u7eaf\u5316\u4f5c\u4e3a\u6700\u4f4e\u7eaf\u5316\u6807\u51c6\u3002<\/li>\n\n\n\n<li>\u5982\u679c\u60a8\u5c06\u5f15\u7269\u7528\u4e8e\u8bf1\u53d8\u5b9e\u9a8c\uff0c\u8bf7\u5c1d\u8bd5\u5c06\u9519\u914d\u78b1\u57fa\u653e\u5728\u5f15\u7269\u4e2d\u95f4\u4f4d\u7f6e\u3002<\/li>\n\n\n\n<li>\u5982\u679c\u60a8\u5c06\u5f15\u7269\u7528\u4e8ePCR\u53cd\u5e94\u5e76\u7528\u4e8eInvitrogen TOPO\u514b\u9686\uff0c\u5f15\u7269\u4e0d\u5e94\u5e26\u6709\u78f7\u9178\u4fee\u9970\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u8bbe\u8ba1\u5be1\u6838\u82f7\u9178\u5e76\u786e\u4fdd\u60a8\u62e5\u6709\u6b63\u786e\u7684\u53c2\u6570\u5be1\u6838\u82f7\u9178\u662f\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u91cd\u8981\u6b65\u9aa4\uff0c\u5c24\u5176\u662f\u5728PCR\u5f15\u7269\u8bbe\u8ba1\u4e2d\u3002\u4e3a\u4e86\u5b9e\u73b0\u6210\u529f\u7684D<\/p>\n","protected":false},"author":1852,"featured_media":886,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[8],"tags":[89],"division":[9],"class_list":{"0":"post-885","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-8","8":"tag-89","9":"division-bid","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>PCR\u5f15\u7269\u8bbe\u8ba1\u6280\u5de7 - \u8d5b\u9ed8\u98de\u4e2d\u6587\u535a\u5ba2<\/title>\n<meta name=\"description\" content=\"\u8bbe\u8ba1\u5be1\u6838\u82f7\u9178\u5e76\u786e\u4fdd\u4e3a\u5be1\u6838\u82f7\u9178\u8bbe\u7f6e\u6b63\u786e\u7684\u53c2\u6570\uff0c\u662f\u83b7\u5f97\u53ef\u9760\u7ed3\u679c\u7684\u91cd\u8981\u6b65\u9aa4\uff0c\u5c24\u5176\u662f\u5728 PCR \u5f15\u7269\u8bbe\u8ba1\u4e2d\u3002\u4e3a\u4e86\u5b9e\u73b0\u6210\u529f\u7684 DNA \u6269\u589e\uff0c\u4ece\u5408\u9002\u7684\u5f15\u7269\u5165\u624b\u81f3\u5173\u91cd\u8981\u3002\u4ec0\u4e48\u6837\u7684\u5f15\u7269\u624d\u662f\u597d\u7684\u5f15\u7269\u5462\uff1f\u4ee5\u4e0b\u662f\u4e00\u4e9b PCR \u8bbe\u8ba1\u7684\u6307\u5bfc\u539f\u5219\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" 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