{"id":898,"date":"2026-03-16T06:17:48","date_gmt":"2026-03-16T06:17:48","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=898"},"modified":"2026-05-13T02:47:40","modified_gmt":"2026-05-13T02:47:40","slug":"hplc-basics-what-you-should-know","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/hplc-basics-what-you-should-know\/","title":{"rendered":"HPLC\u57fa\u7840\u77e5\u8bc6\uff1a\u4f60\u5e94\u8be5\u4e86\u89e3\u7684\u5185\u5bb9"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/download-solution.html?icid=cn-blog-cmd\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1024x176.jpg\" alt=\"\" class=\"wp-image-1799\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/04\/779-\u00d7-134-CMD-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n\n\n<p>\u5728\u5f53\u4eca\u7684\u5206\u6790\u5b9e\u9a8c\u5ba4\u4e2d\uff0c\u4f60\u51e0\u4e4e\u4e0d\u53ef\u80fd\u627e\u4e0d\u5230\u4e00\u53f0<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information\/hplc-basics.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09<\/a>\u4eea\u5668\uff0c\u56e0\u4e3a\u5b83\u5728\u533b\u836f\u3001\u98df\u54c1\u996e\u6599\u3001\u5236\u9020\u548c\u73af\u5883\u5b89\u5168\u7b49\u4f17\u591a\u9886\u57df\u4e2d\u90fd\u626e\u6f14\u7740\u81f3\u5173\u91cd\u8981\u7684\u89d2\u8272\u3002<\/p>\n\n\n\n<p>\u8fd9\u7bc7\u535a\u5ba2\u6587\u7ae0\u89e3\u91ca\u4e86\u4ec0\u4e48\u662f\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u3001\u5176\u5de5\u4f5c\u539f\u7406\uff0c\u4ee5\u53ca\u6db2\u76f8\u8272\u8c31\u4eea\u7528\u4e8e\u7eaf\u5316\u6df7\u5408\u7269\u7684\u4e0d\u540c\u6280\u672f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId-1155097430\"><strong><strong>\u4ec0\u4e48\u662fHPLC\uff1f<\/strong><\/strong><\/h3>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/20762-og-1-300x230.jpg\" alt=\"\" class=\"wp-image-12171\" \/><\/figure>\n<\/div>\n\n\n<p>HPLC\u662f\u4e00\u79cd\u5e7f\u6cdb\u5e94\u7528\u4e8e\u5206\u6790\u5316\u5b66\u7684\u6280\u672f\uff0c\u7528\u4e8e\u5206\u79bb\u3001\u9274\u5b9a\u548c\u5b9a\u91cf\u5316\u5b66\u6df7\u5408\u7269\u4e2d\u7684\u5316\u5408\u7269\u3002\u8fd9\u4e9b\u5206\u79bb\u8fc7\u7a0b\u5229\u7528\u6d41\u52a8\u76f8\u5728\u538b\u529b\u9a71\u52a8\u4e0b\u901a\u8fc7\u586b\u5145\u6709\u56fa\u5b9a\u76f8\u7684\u8272\u8c31\u67f1\u8fdb\u884c\u3002<\/p>\n\n\n\n<p>\u6d41\u52a8\u76f8\u5c06\u6db2\u4f53\u6837\u54c1\u5e26\u5165\u8272\u8c31\u67f1\u5e76\u8f93\u9001\u5230\u68c0\u6d4b\u5668\uff0c\u4e0d\u540c\u5316\u5408\u7269\u2014\u2014\u5373\u5206\u6790\u7269\u2014\u2014\u7531\u4e8e\u4e0e\u56fa\u5b9a\u76f8\u4f5c\u7528\u529b\u7684\u4e0d\u540c\u800c\u5b9e\u73b0\u5206\u79bb\u3002<\/p>\n\n\n\n<p>\u68c0\u6d4b\u5668\u5728\u5206\u6790\u7269\u4ece\u8272\u8c31\u67f1\u6d17\u8131\u540e\u5bf9\u5176\u8fdb\u884c\u6d4b\u91cf\uff0c\u8272\u8c31\u6570\u636e\u7cfb\u7edf\uff08CDS\uff09\u5219\u5c06\u68c0\u6d4b\u5230\u7684\u4fe1\u53f7\u8fdb\u884c\u8f6c\u6362\u3002<\/p>\n\n\n\n<p>HPLC\u5206\u6790\u7684\u7ffb\u8bd1\u6570\u636e\u8f93\u51fa\u79f0\u4e3a\u8272\u8c31\u56fe\uff0c\u5176\u4e2dx\u8f74\u8868\u793a\u65f6\u95f4\uff0cy\u8f74\u662f\u7531\u68c0\u6d4b\u5668\u4ea7\u751f\u7684\u7279\u5b9a\u4fe1\u53f7\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/Chromatogram-1.jpg\" alt=\"\" class=\"wp-image-12244\" \/><\/figure>\n<\/div>\n\n\n<p>\u56fe1. HPLC\u8272\u8c31\u56fe\u793a\u4f8b\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId-2042601111\"><strong><strong>HPLC\u662f\u5982\u4f55\u5de5\u4f5c\u7684\uff1f<\/strong><\/strong><\/h3>\n\n\n\n<p>\u4e00\u53f0HPLC\u4eea\u5668\u901a\u5e38\u6709\u56db\u4e2a\u4e3b\u8981\u786c\u4ef6\u7ec4\u6210\u90e8\u5206\uff1a\u6cf5\u3001\u81ea\u52a8\u8fdb\u6837\u5668\u3001\u8272\u8c31\u67f1\u548c\u68c0\u6d4b\u5668\u3002\u5176\u4ed6\u90e8\u4ef6\u5305\u62ec\u6eb6\u5242\u3001CDS\u8f6f\u4ef6\u5305\uff0c\u4ee5\u53ca\u8fde\u63a5\u6bdb\u7ec6\u7ba1\u548c\u7ba1\u8def\uff0c\u4ee5\u4fbf\u6d41\u52a8\u76f8\u548c\u6837\u54c1\u5728\u7cfb\u7edf\u4e2d\u6301\u7eed\u6d41\u52a8\u3002<\/p>\n\n\n\n<p>\u6bcf\u6b21HPLC\u5206\u6790\u90fd\u5305\u62ec\u4ee5\u4e0b\u6b65\u9aa4\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>\u6d41\u52a8\u76f8\u5f00\u59cb\u6d41\u52a8<\/strong>\u2014 \u6cf5\u4ee5\u6307\u5b9a\u7684\u6d41\u901f\u5c06\u6d17\u8131\u5242\u63a8\u9001\u901a\u8fc7\u7cfb\u7edf\u3002<\/li>\n\n\n\n<li><strong>\u6837\u54c1\u8fdb\u6837<\/strong>\u2014 \u6837\u54c1\u6ce8\u5165\u5230\u6d41\u52a8\u76f8\u540e\uff0c\u4f1a\u968f\u6d41\u52a8\u76f8\u4ece\u8fdb\u6837\u70b9\u79fb\u52a8\u5230\u8272\u8c31\u67f1\u5934\u90e8\u3002<\/li>\n\n\n\n<li><strong>\u5316\u5408\u7269\u5206\u79bb<\/strong>\u2014 \u5316\u5408\u7269\u7684\u7269\u7406\u5206\u79bb\u53d1\u751f\u5728\u67f1\u5b50\u7684\u56fa\u5b9a\u76f8\u4e0a\u3002\u6837\u54c1\u7ec4\u5206\u4ece\u67f1\u5b50\u6d17\u8131\u540e\uff0c\u5206\u79bb\u7684\u7ec4\u5206\u4f1a\u8fdb\u5165\u68c0\u6d4b\u5668\u3002<\/li>\n\n\n\n<li><strong>\u5206\u6790\u7269\u68c0\u6d4b<\/strong>\u2014 \u57fa\u4e8e\u7279\u5b9a\u5c5e\u6027\u4ea7\u751f\u7684\u7535\u4fe1\u53f7\u6765\u68c0\u6d4b\u6307\u5b9a\u7684\u5206\u6790\u7269\u3002<\/li>\n\n\n\n<li><strong>\u8272\u8c31\u56fe\u751f\u6210<\/strong>\u2014 CDS\u5c06\u68c0\u6d4b\u5230\u7684\u5206\u6790\u7269\u4fe1\u53f7\u8f6c\u6362\u4e3a\u5206\u6790\u7269\u4fe1\u53f7\u4e0e\u65f6\u95f4\u5173\u7cfb\u7684\u8272\u8c31\u56fe\u3002<\/li>\n<\/ol>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/HPLC-Learning-Center-Page1-Components-1160x600-2.jpg\" alt=\"\" class=\"wp-image-12245\" \/><\/figure>\n<\/div>\n\n\n<p>\u56fe2\uff1a\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u4eea\u5668\u793a\u610f\u56fe\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId--1364862504\"><strong><strong>\u54ea\u4e9b\u56e0\u7d20\u5f71\u54cdHPLC\u5206\u79bb\uff1f<\/strong><\/strong><\/h3>\n\n\n\n<p>\u8bb8\u591a\u56e0\u7d20\uff0c\u5982\u6d41\u52a8\u76f8\u7ec4\u6210\u3001\u67f1\u5b50\u5316\u5b66\u6027\u8d28\u548c\u6e29\u5ea6\uff0c\u90fd\u53ef\u4ee5\u5f71\u54cdHPLC\u5206\u79bb\u3002\u5982\u679c\u5206\u6790\u7269\u5bf9\u67f1\u5b50\u7684\u4eb2\u548c\u529b\u4e0d\u540c\uff0c\u624d\u80fd\u5b9e\u73b0\u6210\u529f\u5206\u79bb\uff0c\u56e0\u6b64\u9009\u62e9\u9002\u5408\u4f60\u5316\u5408\u7269\u7684\u56fa\u5b9a\u76f8\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n<p>\u5f71\u54cd\u6574\u4f53\u5206\u79bb\u8fc7\u7a0b\u7684\u4e3b\u8981\u56e0\u7d20\u6709\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5206\u6790\u7269\u7684\u7406\u5316\u6027\u8d28\uff0c\u5982\u5927\u5c0f\u3001\u7535\u8377\u3001\u6781\u6027\u548c\u6325\u53d1\u6027<\/li>\n\n\n\n<li>\u56fa\u5b9a\u76f8\u7684\u7406\u5316\u6027\u8d28\uff0c\u5982\u6781\u6027\u3001\u7535\u8377\u548c\u9ecf\u5ea6<\/li>\n\n\n\n<li>\u6240\u7528\u6d41\u52a8\u76f8\u7684\u7406\u5316\u6027\u8d28\u53ca\u5176\u4e0e\u5206\u6790\u7269\u548c\u56fa\u5b9a\u76f8\u7684\u76f8\u4e92\u4f5c\u7528<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId--477358823\"><strong><strong>\u7b49\u5ea6\u5206\u79bb\u4e0e\u68af\u5ea6\u5206\u79bb<\/strong><\/strong><\/h3>\n\n\n\n<p>\u6240\u6709HPLC\u5206\u79bb\u90fd\u4ee5\u4e24\u79cd\u6a21\u5f0f\u4e4b\u4e00\u8fdb\u884c\u2014\u2014\u7b49\u5ea6\u6216\u68af\u5ea6\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u7b49\u5ea6\u65b9\u6cd5\u5728\u5206\u6790\u8fc7\u7a0b\u4e2d\u4f7f\u7528\u6052\u5b9a\u7684\u6d17\u8131\u5242\u7ec4\u6210\u8fdb\u884c\u5206\u79bb\uff0c\u4f8b\u5982100%\u4e59\u8148\u621650:50\u4e59\u8148\u4e0e\u6c34\u7684\u6df7\u5408\u7269\u3002<\/li>\n\n\n\n<li>\u68af\u5ea6\u65b9\u6cd5\u5219\u662f\u5728\u5206\u79bb\u8fc7\u7a0b\u4e2d\u6539\u53d8\u6d41\u52a8\u76f8\u7ec4\u6210\u3002\u8fd9\u4e9b\u65b9\u6cd5\u901a\u5e38\u4f7f\u7528\u4e24\u79cd\u6eb6\u5242\uff0c\u79f0\u4e3aA\u548cB\u3002\u4f8b\u5982\uff0c\u5b9e\u9a8c\u5f00\u59cb\u65f6A:B\u4e3a60%\u6c34\u5bf940%\u4e59\u8148\uff0c\u968f\u540e\u5728\u5206\u79bb\u8fc7\u7a0b\u4e2d\u6bd4\u4f8b\u53d1\u751f\u53d8\u5316\u3002<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/HPLC-Learning-Center-Page1-Isocratic-vs-gradient-1160x600-2.jpg\" alt=\"\" class=\"wp-image-12246\" \/><\/figure>\n<\/div>\n\n\n<p>\u56fe3. \u7b49\u5ea6\u6d17\u8131\u4e0e\u68af\u5ea6\u6d17\u8131\u7684\u793a\u610f\u56fe\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId-410144858\"><strong><strong>\u6db2\u76f8\u8272\u8c31\u6280\u672f\u7c7b\u578b<\/strong><\/strong><\/h3>\n\n\n\n<p>\u9274\u4e8e\u5316\u5408\u7269\u6570\u91cf\u4f17\u591a\u4e14\u6f5c\u5728\u5206\u6790\u7269\u7ed3\u6784\u591a\u6837\uff0cHPLC\u5f88\u5c11\u91c7\u7528\u4e00\u5200\u5207\u7684\u65b9\u6cd5\u3002\u4ece\u7eb3\u5347\u7ea7\u5230\u5236\u5907\u7ea7\u5206\u79bb\uff0c\u4ee5\u4e0b\u662f\u6700\u5e38\u89c1\u7684HPLC\u6280\u672f\u7c7b\u578b\u53ca\u5176\u9002\u7528\u573a\u666f\u5217\u8868\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong><strong>\u6db2\u76f8\u8272\u8c31\u6280\u672f<\/strong><\/strong><\/td><td><strong><strong>\u5de5\u4f5c\u539f\u7406<\/strong><\/strong><\/td><td><strong><strong>\u5e94\u7528<\/strong><\/strong><\/td><\/tr><tr><td><strong><strong>\u9ad8\u6548\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/vanquish-core-hplc-system.html\" target=\"_blank\" rel=\"noreferrer noopener\">HPLC\uff08\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff09<\/a>\u5728\u538b\u529b\u4f4e\u4e8e700\u5df4\u3001\u6d41\u901f\u4e3a1\u20132\u6beb\u5347\/\u5206\u949f\u7684\u6761\u4ef6\u4e0b\u8fd0\u884c<\/td><td>\u7528\u4e8e\u5236\u836f\u3001\u5de5\u4e1a\u5316\u5b66\u54c1\u548c\u98df\u54c1\u5b89\u5168\u9886\u57df\u4e2d\u5c0f\u5206\u5b50\u548c\u5927\u5206\u5b50\u7684\u8d28\u91cf\u4fdd\u8bc1\/\u8d28\u91cf\u7ba1\u7406\uff08QA\/QC\uff09<\/td><\/tr><tr><td><strong><strong>\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/vanquish-horizon-uhplc-system.html\" target=\"_blank\" rel=\"noreferrer noopener\">UHPLC\uff08\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff09<\/a>\u91c7\u7528\u8d85\u8fc71000\u5df4\u7684\u538b\u529b\uff0c\u6d41\u901f\u4e3a0.2\u20130.7\u6beb\u5347\/\u5206\u949f\u3002\u8f83\u9ad8\u7684\u538b\u529b\u8303\u56f4\u53ef\u63d0\u4f9b\u66f4\u597d\u7684\u5206\u8fa8\u7387\u548c\u7075\u654f\u5ea6\u3001\u66f4\u9ad8\u7684\u901a\u91cf\uff0c\u4ee5\u53ca\u6bd4\u6807\u51c6HPLC\u7cfb\u7edf\u66f4\u5c11\u7684\u6eb6\u5242\u6d88\u8017<\/td><td>\u901a\u5e38\u5e94\u7528\u4e8e\u7814\u53d1\u5b9e\u9a8c\u5ba4\u4ee5\u53ca\u5236\u836f\u548c\u751f\u7269\u5236\u836f\u9886\u57df\uff0c\u7528\u4e8e\u5c0f\u5206\u5b50\u836f\u7269\u3001\u80bd\u7c7b\u548c\u6297\u4f53\u7684\u5f00\u53d1\u4e0e\u8868\u5f81<\/td><\/tr><tr><td><strong><strong>\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u8054\u7528\u6280\u672f<\/strong><\/strong><\/td><td>\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u8054\u7528\u6280\u672f\uff08LC-MS\uff09\u91c7\u7528\u4e86<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/mass-spectrometry\/mass-spectrometry-learning-center.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d28\u8c31\u4eea<\/a>\u800c\u4e0d\u662f\u4f20\u7edf\u7684\u5149\u5b66\u68c0\u6d4b\u5668\uff0c\u5982\u7d2b\u5916-\u53ef\u89c1\u68c0\u6d4b\u5668<\/td><td>\u4e3b\u8981\u7528\u4e8e\u80bd\u7c7b\u548c\u86cb\u767d\u8d28\u5206\u6790<\/td><\/tr><tr><td><strong><strong>\u4f4e\u6d41\u901f\u6db2\u76f8\u8272\u8c31\u6cd5<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/products-and-services\/promotions\/industrial\/vanquish-neo-beyond-brilliant.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u4f4e\u6d41\u901f\u6db2\u76f8\u8272\u8c31\u6cd5<\/a>\u6db5\u76d6\u7eb3\u5347\u7ea7\u3001\u5fae\u5347\u7ea7\u548c\u6bdb\u7ec6\u7ba1\u7ea7\u6d41\u901f\u8303\u56f4\uff0c\u4ece\u4f4e\u7eb3\u5347\/\u5206\u949f\u5230\u7ea650\u5fae\u5347\/\u5206\u949f\u3002\u7531\u4e8e\u67f1\u5185\u5f84\u51cf\u5c0f\uff0c\u6837\u54c1\u5e26\u7a00\u91ca\u51cf\u5c11\uff0c\u56e0\u6b64\u8be5\u6280\u672f\u63d0\u9ad8\u4e86\u7075\u654f\u5ea6\u3002<\/td><td>\u975e\u5e38\u9002\u5408\u5728\u590d\u6742\u751f\u7269\u57fa\u8d28\u4e2d\u5bf9\u5206\u5b50\u8fdb\u884c\u9ad8\u7075\u654f\u5ea6\u6d4b\u91cf\uff0c\u5176\u4e2d\u5206\u6790\u7269\u6d53\u5ea6\u53ef\u80fd\u8de8\u8d8a\u51e0\u4e2a\u6570\u91cf\u7ea7<\/td><\/tr><tr><td><strong><strong>\u5236\u5907\u578b\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td>\u5236\u5907\u578b\u6db2\u76f8\u8272\u8c31\u6280\u672f\u5305\u62ec<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/brochures\/sl-001235-lc-vanquish-analytical-purification-sl001235-en.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u6536\u96c6\u5206\u998f\u540e\u7684\u6d17\u8131\u6db2<\/a>\u5c06\u5176\u6536\u96c6\u5230\u72ec\u7acb\u7684\u6837\u54c1\u5bb9\u5668\u4e2d\uff0c\u4ee5\u5206\u79bb\u4e00\u79cd\u6216\u591a\u79cd\u5206\u6790\u7269\uff0c\u4ece\u800c\u7eaf\u5316\u4e3b\u8981\u6210\u5206\u6216\u5206\u79bb\u6742\u8d28\u4ee5\u4fbf\u8fdb\u4e00\u6b65\u7814\u7a76<\/td><td>\u5404\u79cd\u5e94\u7528\uff0c\u5982\u836f\u7269\u7684\u5927\u89c4\u6a21\u7eaf\u5316\uff0c\u6216\u5c0f\u89c4\u6a21\u7528\u4e8e\u63d0\u9ad8\u4ea7\u54c1\u4ea7\u7387\u6216\u5206\u79bb\u7eaf\u51c0\u5316\u5408\u7269<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId-1297648539\"><strong><strong>\u591a\u901a\u9053\u6db2\u76f8\u8272\u8c31\u7cfb\u7edf<\/strong><\/strong><\/h3>\n\n\n\n<p>\u591a\u901a\u9053\u6db2\u76f8\u8272\u8c31\u7cfb\u7edf\u91c7\u7528\u591a\u4e2a\u6d41\u8def\uff0c\u5e2e\u52a9\u8272\u8c31\u5206\u6790\u4eba\u5458\u8fdb\u884c\u590d\u6742\u548c\/\u6216\u5e76\u884c\u7684\u5206\u79bb\u8fc7\u7a0b\u3002\u591a\u901a\u9053\u7cfb\u7edf\u7684\u4f18\u52bf\u5305\u62ec\u66f4\u9ad8\u7684\u6837\u54c1\u5904\u7406\u91cf\u3001\u66f4\u597d\u5730\u89e3\u6790\u590d\u6742\u6837\u54c1\uff0c\u4ee5\u53ca\u589e\u5f3a\u5206\u6790\u7269\u5b9a\u91cf\u80fd\u529b<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong><strong>\u591a\u901a\u9053\u6db2\u76f8\u8272\u8c31\u6280\u672f<\/strong><\/strong><\/td><td><strong><strong>\u5de5\u4f5c\u539f\u7406<\/strong><\/strong><\/td><td><strong><strong>\u5e94\u7528<\/strong><\/strong><\/td><\/tr><tr><td><strong><strong>\u4e8c\u7ef4\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/products-and-services\/promotions\/industrial\/vanquish-online-2d-lc-systems.html\" target=\"_blank\" rel=\"noreferrer noopener\">2D-LC<\/a> \u662f\u4e00\u79cd\u5148\u8fdb\u7684\u5206\u79bb\u6280\u672f\uff0c\u901a\u8fc7\u4e24\u79cd\u4e92\u8865\u7684\u8272\u8c31\u67f1\u5316\u5b66\u6027\u8d28\uff0c\u5b9e\u73b0\u591a\u7ef4\u5206\u79bb\uff0c\u800c\u4e0d\u662f\u4ec5\u901a\u8fc7\u5355\u4e00\u8272\u8c31\u67f1\u5bf9\u6837\u54c1\u8fdb\u884c\u5206\u79bb\u3002<\/td><td>\u4e8c\u7ef4\u6db2\u76f8\u8272\u8c31\u53ef\u5e94\u7528\u4e8e\u590d\u6742\u5316\u5b66\u6df7\u5408\u7269\uff0c\u5982\u75ab\u82d7\u548c\u98df\u54c1\u4e2d\u5b58\u5728\u5e72\u6270\u57fa\u8d28\u7684\u6837\u54c1\u3002<\/td><\/tr><tr><td><strong><strong>\u53cc\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/vanquish-duo-uhplc-dual-lc-system.html\" target=\"_blank\" rel=\"noreferrer noopener\">Dual LC\uff08\u53ccLC\uff09<\/a> \u662f\u4e00\u79cd\u591a\u901a\u9053\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u65b9\u6cd5\uff0c\u5728\u5355\u4e00\u7cfb\u7edf\u4e2d\u4f7f\u7528\u4e24\u4e2a\u72ec\u7acb\u6d41\u8def\uff0c\u53ef\u540c\u65f6\u8fdb\u884c\u4e24\u9879\u5206\u6790\u3002<\/td><td>\u9002\u7528\u4e8e\u9700\u8981\u63d0\u9ad8\u6837\u54c1\u901a\u91cf\u7684\u60c5\u51b5\uff0c\u4f8b\u5982\u5728\u4e00\u6b21\u8fd0\u884c\u4e2d\u540c\u65f6\u5206\u6790\u6837\u54c1\u4e2d\u7684\u519c\u836f\u548c\u915a\u7c7b\uff0c\u6216\u540c\u6b65\u8fdb\u884c\u91cd\u590d\u5206\u6790\u3002<\/td><\/tr><tr><td><strong><strong>\u4e32\u8054\u6db2\u76f8\u8272\u8c31<\/strong><\/strong><\/td><td><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/vanquish-duo-uhplc-tandem-lc-system.html\" target=\"_blank\" rel=\"noreferrer noopener\">Tandem LC <\/a>\u8fd9\u4e9b\u6280\u672f\u4f7f\u7528\u7b2c\u4e8c\u4e2a\u6cf5\u548c\u667a\u80fd\u67f1\u5207\u6362\uff0c\u901a\u8fc7\u51cf\u5c11\u67f1\u5b50\u518d\u751f\u7684\u505c\u673a\u65f6\u95f4\u6765\u6700\u5927\u5316\u68c0\u6d4b\u5668\u7684\u4f7f\u7528\u7387\u3002<\/td><td>\u6700\u9002\u7528\u4e8e\u836f\u7269\u53d1\u73b0\u5b9e\u9a8c\u5ba4\u7684\u5148\u5bfc\u5316\u5408\u7269\u7b5b\u9009\uff0c\u4ee5\u63d0\u9ad8\u6837\u54c1\u901a\u91cf\u5e76\u6700\u5927\u5316\u68c0\u6d4b\u5668\u5229\u7528\u7387\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"toc-hId--2109815076\"><strong><strong>\u60f3\u4e86\u89e3\u66f4\u591a\u5173\u4e8eHPLC\u5206\u6790\u7684\u4fe1\u606f\u5417\uff1f<\/strong><\/strong><\/h3>\n\n\n\n<p>\u770b\u5230\u6db2\u76f8\u8272\u8c31\u7684\u53d1\u5c55\u4ee4\u4eba\u96be\u4ee5\u7f6e\u4fe1\u3002<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/mass-spectrometry\/mass-spectrometry-learning-center\/liquid-chromatography-mass-spectrometry-lc-ms-information.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u6db2\u76f8\u8272\u8c31<\/a>\u5728\u73b0\u5b9e\u65f6\u95f4\u4e2d\u3002\u4ece\u4e00\u5f00\u59cb\u5206\u79bb\u8272\u7d20\uff0c\u5230\u57fa\u7840\u7684\u6db2\u76f8\u8272\u8c31\uff0c\u8fd9\u66fe\u662f\u4e00\u4e2a\u975e\u5e38\u624b\u52a8\u4e14\u8017\u65f6\u6570\u5c0f\u65f6\u624d\u80fd\u5206\u79bb\u76ee\u6807\u5316\u5408\u7269\u7684\u8fc7\u7a0b\u3002\u5982\u4eca\uff0c\u501f\u52a9\u73b0\u4ee3HPLC\u548cUHPLC\u4eea\u5668\uff0c\u5206\u79bb\u8fc7\u7a0b\u53ea\u9700\u51e0\u5206\u949f\uff0c\u751a\u81f3\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\u53ea\u9700\u51e0\u79d2\u949f\u3002<\/p>\n\n\n\n<p>\u6b64\u5916\uff0c\u901a\u8fc7\u7ed3\u5408\u7d2b\u5916\u68c0\u6d4b\uff08UV\uff09\u3001\u8d28\u8c31\uff08MS\uff09\u4ee5\u53ca<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information\/hplc-system-components\/how-hplc-detectors-work\/charged-aerosol-detection.html?cid=fl-cad\" target=\"_blank\" rel=\"noreferrer noopener\">\u5e26\u7535\u6c14\u6eb6\u80f6\u68c0\u6d4b\u5668\uff08CAD\uff09<\/a>\u7b49\u591a\u79cd\u68c0\u6d4b\u6280\u672f\uff0c\u5bf9\u5316\u5408\u7269\u8fdb\u884c\u68c0\u6d4b\u3001\u9274\u5b9a\u4e0e\u5b9a\u91cf\u5206\u6790\u53d8\u5f97\u524d\u6240\u672a\u6709\u5730\u9ad8\u6548\u4e0e\u4fbf\u6377\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u89c2\u770b\uff1a<a href=\"https:\/\/www.youtube.com\/watch?v=WRum8Fddec8\" target=\"_blank\" rel=\"noreferrer noopener\">\u8d5b\u9ed8\u98de\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u7684\u8fdb\u5316\u4e4b\u65c5<\/a>\u3002<\/li>\n\n\n\n<li>\u8bbf\u95ee\u6211\u4eec\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/chromatography-learning-center\/liquid-chromatography-information.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u5b66\u4e60\u4e2d\u5fc3<\/a>\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u7684\u4fe1\u606f\uff0c\u5e76\u83b7\u53d6\u6211\u4eec\u7684<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/3d-tours.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u865a\u62dfHPLC\u4eea\u5668\u4ea7\u54c1\u5bfc\u89c8<\/a>\u3002<\/li>\n\n\n\n<li>\u522b\u5fd8\u4e86\u5173\u6ce8\u6211\u4eec\u7684<a href=\"https:\/\/www.linkedin.com\/showcase\/chromatography-&amp;-mass-spectrometry\/\" target=\"_blank\" rel=\"noreferrer noopener\">LinkedIn\u4e0a\u7684\u8272\u8c31\u4e0e\u8d28\u8c31\u9875\u9762<\/a>\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u5728\u5f53\u4eca\u7684\u5206\u6790\u5b9e\u9a8c\u5ba4\u4e2d\uff0c\u4f60\u51e0\u4e4e\u4e0d\u53ef\u80fd\u627e\u4e0d\u5230\u4e00\u53f0\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u4eea\u5668\uff0c\u56e0\u4e3a\u5b83\u5728\u533b\u836f\u3001\u98df\u54c1\u996e\u6599\u3001\u5236\u9020\u548c\u73af\u5883\u5b89<\/p>\n","protected":false},"author":1852,"featured_media":899,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[49],"tags":[74],"division":[50],"class_list":{"0":"post-898","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-49","8":"tag-hplc","9":"division-cmd","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>HPLC\u57fa\u7840\u77e5\u8bc6\uff1a\u4f60\u5e94\u8be5\u4e86\u89e3\u7684\u5185\u5bb9 - \u8d5b\u9ed8\u98de\u4e2d\u6587\u535a\u5ba2<\/title>\n<meta name=\"description\" content=\"\u8fd9\u7bc7\u535a\u5ba2\u6587\u7ae0\u89e3\u91ca\u4e86\u4ec0\u4e48\u662f\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u3001\u5176\u5de5\u4f5c\u539f\u7406\uff0c\u4ee5\u53ca\u6db2\u76f8\u8272\u8c31\u4eea\u7528\u4e8e\u7eaf\u5316\u6df7\u5408\u7269\u7684\u4e0d\u540c\u6280\u672f\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.cn\/blog\/accelerating-science-in-china\/hplc-basics-what-you-should-know\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta 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