{"id":969,"date":"2026-03-16T07:44:34","date_gmt":"2026-03-16T07:44:34","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=969"},"modified":"2026-04-22T21:59:31","modified_gmt":"2026-04-22T21:59:31","slug":"exploring-the-mysteries-of-cell-phagocytosis","status":"publish","type":"post","link":"https:\/\/www.thermofisher.cn\/blog\/exploring-the-mysteries-of-cell-phagocytosis\/","title":{"rendered":"pHrodo\u6807\u8bb0\u8bd5\u5242\u63a2\u7d22\u7ec6\u80de\u541e\u566c\u7684\u5965\u79d8"},"content":{"rendered":"\n

\u4e0a\u4e00\u671f\u7684\u63a8\u6587\u4e2d\uff0c\u6211\u4eec\u4ecb\u7ecd\u4e86\u8102\u4ee3\u8c22\u7814\u7a76\u8fc7\u7a0b\u4e2d\uff0c\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\uff08LDL\uff09\u7684\u7c7b\u578b\u4e0e\u5404\u7c7b\u8367\u5149\u6807\u8bb0LDL\u3002\u5176\u4e2d\u6211\u4eec\u63d0\u5230\u4e86\u4e00\u7c7b\u7279\u6b8a\u7684\u8367\u5149\u57fa\u56e2pHrodo\u2122\uff0c\u90a3\u4e48pHrodo\u2122\u6807\u8bb0\u7684LDL\u6709\u4ec0\u4e48\u7279\u522b\u5e94\u7528\u5462\uff1f\u4ec0\u4e48\u662fpHrodo\u2122\u5462\uff1f\u9664\u4e86\u6807\u8bb0LDL\u4ee5\u5916\uff0cpHrodo\u2122\u662f\u5426\u8fd8\u53ef\u4ee5\u7528\u5728\u5176\u4ed6\u5730\u65b9\u5462\uff1f\u8ddf\u968fDr.\u8d5b\uff0c\u8ba9\u6211\u4eec\u4e00\u63a2\u7a76\u7adf\u3002<\/p>\n\n\n\n

pHrodo\u2122\u6807\u8bb0\u7684LDL\u5728\u8102\u4ee3\u8c22\u7814\u7a76\u5e94\u7528<\/strong><\/h2>\n\n\n\n

\u7ec6\u80de\u5185\u7684\u80c6\u56fa\u9187\u7a33\u6001\u6d89\u53ca\u80c6\u56fa\u9187\u7684\u4ee3\u8c22\u6d88\u8017\u4e0e\u8f6c\u8fd0\u5408\u6210\u3002\u5176\u4e2d\uff0c\u80c6\u56fa\u9187\u5728\u673a\u4f53\u5185\u7684\u8f6c\u8fd0\u4f9d\u8d56\u8102\u86cb\u767d\uff0c\u56e0\u6b64\uff0c\u901a\u8fc7\u8ffd\u8e2a\u8367\u5149\u6807\u8bb0\u7684\u8102\u86cb\u767d\u7684\u6444\u53d6\u53ef\u4ee5\u8bc4\u4f30\u80c6\u56fa\u9187\u5728\u673a\u4f53\u5185\u7684\u6444\u53d6\u8f6c\u8fd0\u80fd\u529b\u3002\u7814\u7a76\u4eba\u5458\u4e3a\u4e86\u7814\u7a76Zeb1\u86cb\u767d\u5728\u7ec6\u80de\u5185\u80c6\u56fa\u9187\u8f6c\u8fd0\u4e2d\u7684\u6f5c\u5728\u4f5c\u7528\uff0c\u5c06Zeb1WT<\/sup>\u548cZeb1\u0394M<\/sup>\u7684\u5de8\u566c\u7ec6\u80de\u5206\u522b\u4e0epHrodo\u2122 Green\u5076\u8054\u7684LDL\uff08pHrodo\u2122 Green-LDL\uff09\u5171\u57f9\u517b\uff0c\u5e76\u4f7f\u7528\u5171\u805a\u7126\u663e\u5fae\u955c\u76d1\u6d4b\u5176\u8367\u5149\u968f\u65f6\u95f4\u7684\u53d8\u5316\u3002pHrodo\u2122 Green\u5728\u7ec6\u80de\u5916\u4e2d\u6027pH\u503c\u4e0b\u5448\u5f31\u8367\u5149\u6216\u65e0\u8367\u5149\uff0c\u4f46\u5728\u56ca\u6ce1\u4ece\u5185\u4f53\u5411\u6eb6\u9176\u4f53\u6210\u719f\u8fc7\u7a0b\u4e2d\uff0c\u968f\u7740pH\u503c\u7684\u4e0b\u964d\uff0c\u5176\u8367\u5149\u5f3a\u5ea6\u4f1a\u9010\u6e10\u589e\u52a0\u3002\u5728\u6700\u521d\u76845\u5206\u949f\u5185\uff0cpHrodo\u2122 Green-LDL\u5728Zeb1WT<\/sup>\u548cZeb1\u0394M<\/sup>\u5de8\u566c\u7ec6\u80de\u4e2d\u7684\u8367\u5149\u6c34\u5e73\u76f8\u5f53\u3002\u7136\u800c\uff0c10\u5206\u949f\u540e\uff0cZeb1\u0394M<\/sup>\u5de8\u566c\u7ec6\u80de\u4e2d\u7684\u8367\u5149\u663e\u8457\u589e\u5f3a\uff0c\u800c\u5728Zeb1WT<\/sup>\u5de8\u566c\u7ec6\u80de\u4e2d\u5219\u4fdd\u6301\u76f8\u5bf9\u7a33\u5b9a\uff08\u56fe1\uff09\u3002\u8fd9\u8868\u660eZeb1\u0394M<\/sup>\u5de8\u566c\u7ec6\u80de\u5185\u7684LDL\u8fd0\u8f93\u53d7\u5230\u963b\u788d\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe1. Zeb1\u7f3a\u5931\u963b\u788d\u5de8\u566c\u7ec6\u80de\u5185\u7684\u8102\u8d28\u8fd0\u8f93[1]<\/sup><\/p>\n\n\n\n

pHrodo\u2122\u6307\u793a\u5242\u662f\u4ec0\u4e48<\/strong><\/h2>\n\n\n\n

\u4e0a\u8ff0\u6587\u732e\u4e2d\u63d0\u5230pHrodo\u2122\u67d3\u6599\u5728\u4e2d\u6027pH\u503c\u65f6\u51e0\u4e4e\u4e0d\u53d1\u8367\u5149<\/strong>\uff0c\u800c\u5728\u9178\u6027\u73af\u5883\u4e0b\u53ef\u53d1\u51fa\u660e\u4eae\u7684\u8367\u5149\uff0c\u8fd9\u79cdpH\u654f\u611f\u6027\u7684\u7279\u6027\u4f7fpHrodo\u2122\u6210\u4e3a\u8bb8\u591a\u5e94\u7528\u7684\u7406\u60f3pH\u6307\u793a\u5242\u3002pHrodo\u2122\u67d3\u6599\u53ef\u7528\u4e8e\u68c0\u6d4b\u7ec6\u80de\u8d28\u6216\u7ec6\u80de\u4e9a\u7ed3\u6784\u5185\u7684pH\u3002\u7531\u4e8e\u5176\u5728\u4e2d\u6027pH\u503c\u4e0b\u51e0\u4e4e\u4e0d\u53d1\u8367\u5149\uff0c\u5e76\u4e14\u4efb\u4f55\u672a\u5185\u5316\u7684\u7684\u67d3\u6599\u57fa\u672c\u4e5f\u4e0d\u53d1\u8367\u5149\uff0c\u56e0\u6b64\u7701\u53bb\u4e86\u6d17\u6da4\u6b65\u9aa4\u548c\u67d3\u6599\u6dec\u706d\u7684\u5fc5\u8981\u3002\u57fa\u4e8e\u8fd9\u79cd\u7279\u6b8a\u7684\u8367\u5149\u7279\u6027\uff0c\u4f7f\u5f97pHrodo\u2122\u5728\u5404\u7c7b\u541e\u566c\u7b49\u5b9e\u9a8c\u4e2d\u6709\u5e7f\u6cdb\u5e94\u7528\u3002<\/p>\n\n\n\n

\u9664\u4e86pHrodo\u2122 Green\u4ee5\u5916\uff0c\u6211\u4eec\u4e5f\u6709\u5176\u4ed6\u989c\u8272\u7684pHrodo\u2122\u57fa\u56e2\uff08\u56fe2\uff09\uff0c\u53ef\u4ee5\u9002\u7528\u4e8e\u4e0d\u540c\u591a\u8272\u8367\u5149\u5171\u67d3\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe2. pHrodo\u2122 Green\u3001Red\u548cDeep Red\u7684\u8367\u5149\u53d1\u5c04\u5149\u8c31<\/p>\n\n\n\n

\u9897\u7c92\u548c\u86cb\u767d\u7ecf\u8fc7\u7ec6\u80de\u7684\u5185\u5316\u4f5c\u7528\u8fdb\u5165\u56ca\u6ce1\uff0c\u8fd9\u4e9b\u56ca\u6ce1\u76f8\u5bf9\u4e8e\u7ec6\u80de\u5916\u73af\u5883\u800c\u8a00\u662f\u9178\u6027\u7684\u3002\u5229\u7528pH\u654f\u611f\u7684\u8367\u5149\u63a2\u9488\u6807\u8bb0\u8fd9\u4e9b\u9897\u7c92\u6216\u86cb\u767d\uff0c\u53ef\u4ee5\u76d1\u6d4b\u7ec6\u80de\u6444\u53d6\u548c\u5185\u5316\u7684\u591a\u79cd\u8fc7\u7a0b\uff0c\u5e76\u8ffd\u8e2a\u4ece\u65e9\u671f\u5185\u4f53(pH 6.3)\u81f3\u6eb6\u9176\u4f53(pH 4.7)\u6216\u518d\u5faa\u73af\u7684\u6574\u4e2a\u8fc7\u7a0b\uff0c\u4e14\u65e0\u9700\u6dec\u706d\u6216\u6d17\u6da4\u6b65\u9aa4<\/strong>\u3002\u56e0\u6b64\uff0c\u5728\u7ec6\u80de\u541e\u566c\u80fd\u529b\u7684\u76f8\u5173\u7814\u7a76\u4e2d\uff0c\u4e5f\u80fd\u770b\u5230pHrodo\u2122\u7cfb\u5217\u67d3\u6599\u7684\u5e94\u7528\u3002<\/p>\n\n\n\n

\u541e\u566c\u4f5c\u7528<\/strong><\/h2>\n\n\n\n

\u5728\u6b63\u5e38\u7ec6\u80de\u751f\u547d\u6d3b\u52a8\u8fc7\u7a0b\u4e2d\uff0c\u7ec6\u80de\u4f1a\u5185\u5316\u9897\u7c92\u7269\u8d28\uff0c\u5982\u5fae\u751f\u7269\uff0c\u8fd9\u4e00\u8fc7\u7a0b\u5bf9\u4e8e\u514d\u75ab\u53cd\u5e94\u548c\u51cb\u4ea1\u7ec6\u80de\u6e05\u9664\u8fc7\u7a0b\u975e\u5e38\u91cd\u8981\u3002\u541e\u566c\u4f5c\u7528\u7814\u7a76\u7684\u6307\u793a\u5242\u5305\u62ecBioParticles\u7ed3\u5408\u7269\uff0c\u5373\u8367\u5149\u67d3\u6599\u6807\u8bb0\u7684\u5904\u7406\u8fc7\u7684\u5927\u80a0\u6746\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u9175\u6bcd\u805a\u7cd6A\u3002\u4e0d\u540c\u7684\u7ec6\u80de\u4e0a\u53c2\u4e0e\u541e\u566c\u8fc7\u7a0b\u7684\u53d7\u4f53\u4e0d\u540c\uff0c\u56e0\u6b64\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u5bf9\u4e0d\u540c\u6765\u6e90\u7684Bioparticles\u7684\u6444\u53d6\u5b58\u5728\u663e\u8457\u5dee\u5f02\u3002\u5982\u56fe3\u6240\u793a\uff0c\u6587\u7ae0\u6bd4\u8f83\u4e86\u539f\u4ee3\u5de8\u566c\u7ec6\u80de\u5bf9\u4e0d\u540c\u7c7b\u578b\u7684\u9897\u7c92\u7684\u6444\u53d6\u5b58\u5728\u663e\u8457\u5dee\u5f02\u3002\u5c3d\u7ba1\u539f\u4ee3\u5de8\u566c\u7ec6\u80de\u5bf9\u5927\u80a0\u6746\u83cc\u3001\u7ec6\u83cc\u548c\u9175\u6bcd\u9897\u7c92\u7684\u6444\u53d6\u5747\u5b58\u5728\u6d53\u5ea6\u4f9d\u8d56\u6027\uff0c\u4f46\u5bf9\u9175\u6bcd\u9897\u7c92\uff0c\u5c24\u5176\u662f\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u7684\u541e\u566c\u6548\u7387\u66f4\u9ad8\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe3. \u539f\u4ee3\u4eba\u7c7b\u5de8\u566c\u7ec6\u80de\u5bf9\u5927\u80a0\u6746\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u9175\u6bcd\u805a\u7cd6A\u7684\u541e\u566c\u4f5c\u7528[2]<\/sup><\/p>\n\n\n\n

\u7c7b\u4f3c\u5730\uff0c\u6211\u4eec\u4e5f\u53ef\u4ee5\u901a\u8fc7\u5f71\u50cf\u7684\u624b\u6bb5\uff0c\u8ffd\u8e2a\u4e0d\u540c\u57f9\u517b\u6761\u4ef6\u4e0b\uff0c\u7ec6\u80de\u541e\u566c\u80fd\u529b\u7684\u5dee\u5f02\u3002\u6709\u6587\u7ae0\u6bd4\u8f83\u4e86Raw264.7\u7ec6\u80de\uff0c\u5728\u6dfb\u52a0\u7ec6\u80de\u5916\u56ca\u6ce1\uff08bacteria\u2011released extracellular vesicles\uff0cBEVs\uff09\u5171\u57f9\u517b\u540e\uff0c\u80fd\u663e\u8457\u589e\u5f3a\u5176\u541e\u566c\u80fd\u529b\uff08\u56fe4\uff09\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe4. BEV\u5bf9\u5de8\u566c\u7ec6\u80de\u541e\u566c\u4f5c\u7528\u7684\u5f71\u54cd[3]<\/sup><\/p>\n\n\n\n

\u5185\u541e\u4f5c\u7528\u548c\u80de\u996e\u4f5c\u7528<\/strong><\/h2>\n\n\n\n

\u5185\u541e\u4f5c\u7528\u662f\u6307\u7ec6\u80de\u901a\u8fc7\u5185\u541e\u86cb\u767d\u8d28\u6216\u591a\u7cd6\u7b49\u975e\u9897\u7c92\u7269\u8d28\u800c\u5c06\u5176\u5185\u5316\u7684\u8fc7\u7a0b\u3002\u8be5\u8fc7\u7a0b\u5bf9\u4e8e\u7ec6\u80de\u4ee3\u8c22\u548c\u7ec6\u80de\u4fe1\u53f7\u975e\u5e38\u91cd\u8981\u3002\u8367\u5149\u86cb\u767d\u548c\u6709\u673a\u67d3\u6599\u662f\u5bf9\u56ca\u6ce1\u58c1\u548c\u56ca\u6ce1\u5185\u5bb9\u7269\u8fdb\u884c\u67d3\u8272\u7684\u975e\u5e38\u6709\u7528\u7684\u6307\u793a\u5242\u3002\u7531\u4e8e\u56ca\u6ce1pH\u4f1a\u5728\u5185\u541e\u8fc7\u7a0b\u4e2d\u53d1\u751f\u6539\u53d8\uff0c\u56e0\u6b64\uff0c\u4e5f\u53ef\u5229\u7528pH\u6307\u793a\u5242\u68c0\u6d4b\u5185\u541e\u4f5c\u7528\u548c\u80de\u996e\u4f5c\u7528\u7684\u6574\u4e2a\u8fdb\u7a0b\u3002\u76ee\u524d\uff0c\u8ffd\u8e2a\u8367\u5149\u6807\u8bb0\u7684\u8461\u805a\u7cd6\u7684\u5185\u5316\u662f\u5206\u6790\u5185\u541e\u4f5c\u7528\u7684\u5e38\u89c4\u65b9\u6cd5\u4e4b\u4e00\u3002\u7ec6\u80de\u819c\u7684\u5f20\u529b\u4e0e\u7ec6\u80de\u7684\u5185\u541e\u80fd\u529b\u5448\u73b0\u4e00\u5b9a\u76f8\u5173\u6027\uff0c\u6709\u6587\u7ae0\u6bd4\u8f83\u4e86\u836f\u7269\u5904\u7406\u5c0f\u9f20\u80da\u80ce\u5e72\u7ec6\u80de\uff08embryonic stem cells\uff0cESCs\uff0924h\u540e\uff0c\u7ec6\u80de\u5f62\u6001\u6539\u53d8\uff0c\u7ec6\u80de\u819c\u8868\u9762\u5f20\u529b\u589e\u5927\uff0c\u7ec6\u80de\u5185\u541e\u80fd\u529b\u4e5f\u589e\u5f3a\uff08\u56fe5\uff09\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe5. \u4f7f\u7528pHrodo\u2122\u6807\u8bb0\u8461\u805a\u7cd6\u5bf9\u5185\u541e\u4f5c\u7528\u5b9a\u91cf[4]<\/sup><\/p>\n\n\n\n

\u914d\u4f53\u5185\u5316\u4e0e\u6297\u4f53\u5185\u5316<\/strong><\/h2>\n\n\n\n

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\u6211\u4eec\u4e5f\u6709\u603b\u7ed3\u5e94\u7528\u5728\u6297\u4f53\u5076\u8054\u836f\u7269\/ADC\u9886\u57df\uff0c\u5404\u7c7b\u4e0d\u540cpHrodo\u6807\u8bb0\u8bd5\u5242\u7684\u5dee\u5f02\u4e0e\u9009\u62e9\u6307\u5357\uff0c\u8bf7\u8be6\u7ec6\u53c2\u8003\uff1a\u3010\u6297\u4f53\u5076\u8054\u836f\u7269\/ADC\u3011\u7b5b\u9009\u4e0e\u9274\u5b9a\u5de5\u5177\u6c47\u603b<\/a><\/p>\n\n\n\n

\u53c2\u8003\u6587\u732e<\/strong><\/p>\n\n\n\n

1. Martinez-Campanario, M. C., et al. “Atherosclerotic plaque development in mice is enhanced by myeloid ZEB1 downregulation.” Nature Communications 14.1 (2023): 8316.<\/p>\n\n\n\n

2. Lindner, Benjamin, Tobias Burkard, and Michael Schuler. “Phagocytosis assays with different pH-sensitive fluorescent particles and various readouts.” Biotechniques 68.5 (2020): 245-250.<\/p>\n\n\n\n

3. Zhu, Ruiyao, et al. “Probiotic bacteria-released extracellular vesicles enhance macrophage phagocytosis in polymicrobial sepsis by activating the FPR1\/2 pathway.” Molecular Medicine 30.1 (2024): 216.<\/p>\n\n\n\n

4. De Belly, Henry, et al. “Membrane tension gates ERK-mediated regulation of pluripotent cell fate.” Cell stem cell 28.2 (2021): 273-284.<\/p>\n\n\n\n

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