Invitrogen

Aiolos Monoclonal Antibody (8B2), eBioscience™

Name: Aiolos Monoclonal Antibody (8B2), eBioscience\u2122
Brand: Invitrogen
SKU: 14-5789-82

2个已发表图片

5 篇参考文献

查看其他17个Aiolos (IKZF3)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (2)

图: 1 / 3

Aiolos Antibody (14-5789-82) in WB

Immunoblotting of reduced cell lysate prepared from mouse splenocytes with 5 µg/mL of Anti-Mouse Aiolos antibody. Bands were visualized using Anti-Mouse IgG HRP.

Aiolos Antibody in Flow Cytometry (Flow)

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产品信息

14-5789-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

8B2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10734227

产品详细信息

Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.

Applications Reported: This 8B2 antibody has been reported for use in immunoblotting/western blotting.

Applications Tested: This 8B2 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse splenocytes. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。