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Invitrogen
Calpastatin Monoclonal Antibody (1F7E3D10)
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Calpastatin Antibody (MA3-944) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mon... View More
产品信息
MA3-944
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Pig,
Rat
已发表种属
Bovine,
Fish,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1F7E3D10
抗原
Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-944 detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.
MA3-944 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins from bovine skeletal muscle extract which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells with MA3-944 results in diffuse cytoplasmic staining.
The MA3-944 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpastatin. This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.
靶标信息
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Calpain inhibitor; Calpastatin; calpastatin isoform III; calpastatin type 1; calpastatin type 2; calpastatin type I; calpastatin type II; calpastatin type III; calpastatin type IV; heart calpastatin; Sperm BS-17 component
基因别名: BS-17; CAST; PLACK
UniProt ID: (Human) Q96D08, (Rat) P27321
Entrez Gene ID: (Human) 831, (Bovine) 281039, (Pig) 397135, (Rat) 25403
liver development
egg activation
brain development
myoblast fusion
aging
negative regulation of endopeptidase activity
organ regeneration
myoblast differentiation
negative regulation of cell cycle arrest
cell-cell adhesion
negative regulation of type B pancreatic cell apoptotic process
negative regulation of peptidase activity
regulation of protein catabolic process
negative regulation of catalytic activity
