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Invitrogen
ERK2 Polyclonal Antibody
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ERK2 Antibody (PA1-37640) in ICC/IF
Immunofluorescence analysis of ERK2 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK2 Rabbit Polyclonal Antibody (Product # PA5-32396) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
产品信息
PA1-37640
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the C-terminus of mouse ERK-2 protein
偶联物
形式
Liquid
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with bovine, chicken, canine, amphibian, porcine, rabbit and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
靶标信息
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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