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Agrisera
FDX1/Ferredoxin Polyclonal Antibody
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FIGURE: 1 / 2
FDX1/Ferredoxin Antibody (AS06-121) in WB
20 or 40 µg of total protein from Arabidopsis thaliana (WT) leaves or ferredoxin mutant fd 2 were separated on 15% acrylamide gel with 6 M urea. Filters were blotted on PVDF, 5 µg of soluble protein extracts from Chlamydomonas reinhardtii strain CC-4,532 were separated on 15% SDS-PAGE and blotted at 4 W for 30 min to nitrocellulose membrane (0.1 µm pore size) using semi-dry transfer. The blot was blocked with 1% non-fat dry milk overnight at room temperature (RT) with agitation. The blot was incubated in the primary antibody solution at a ... View More
Product Details
AS06-121
Product Specifications
Species Reactivity
Algae,
Bacteria,
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Native ferredoxin purified from Spinacia oleracea.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Pinus strobus, Spinacia oleracea, Synechocystis 6803 substrain PCC-M
Target Information
Ferredoxin 1 (FDX1) encodes a nuclear-encoded mitochondrial protein that is synthesized as a precursor with an N-terminal mitochondrial targeting sequence and is imported into the mitochondrial matrix, where it functions as a small soluble electron-transfer factor. Structurally, FDX1 is a ferredoxin-type iron-sulfur protein in the mitochondrial 2Fe-2S ferredoxin family, binding a 2Fe-2S cluster via conserved cysteine residues within a ferredoxin fold that supports reversible redox cycling and transient protein-protein interactions. Biologically, FDX1 shuttles electrons from the NADPH-dependent ferredoxin reductase (FDXR) to multiple mitochondrial type I cytochrome P450 enzymes, thereby supporting key monooxygenase reactions central to steroid hormone biosynthesis and related pathways (including steps in vitamin D and bile acid metabolism). Beyond steroidogenic P450 support, experimental evidence indicates FDX1 has distinct target specificity from the paralog FDX2 and can contribute electrons to additional mitochondrial biosynthetic processes, including heme a and lipoyl cofactor biosynthesis, consistent with a broader role in mitochondrial redox chemistry and respiratory competence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: At1g10960; ATFD1; FD1; PETF2; T19D16.12; T19D16_12
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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