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GPNMB Antibody (740041MP555) in ICC/IF
Immunofluorescence analysis of GPNMB was performed using 70% confluent SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GPNMB Recombinant Mouse Monoclonal Antibody (HOST5DS), Alexa Fluor™ Plus 555 (Product # 740041MP555, 1:100) at 4 degree Celsius overnight. Panel a) shows representative images of cells that were stained for detection and localization of GPNMB. Panel b) shows ... View More
产品信息
740041MP555
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HOST5DS
抗原
Fc-tagged full protein expressed in insect cells
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This HOST5DS antibody has been pre-titrated and tested by flow cytometric analysis of human blood monocyte-derived macrophages. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Tissue dissociation protocols involving enzymatic digestion should be optimized to limit proteolytic degradation of GPNMB on the cell surface.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; osteoactivin; transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
基因别名: GPNMB; HGFIN; NMB; UNQ1725/PRO9925
UniProt ID: (Human) Q14956
Entrez Gene ID: (Human) 10457
