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Glutaminase Antibody (710997) in ICC/IF
Immunofluorescence analysis of GLS was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Glutaminase Recombinant Rabbit Superclonal™ Antibody (6HCLC) (Product # 710997) at 5 µg/mL in 0.1% BSA, incubated at ... View More
产品信息
710997
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Virus
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
6HCLC
抗原
Protein corresponding to Human GLS (aa251-533)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Rat and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
Glutaminase is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing neurotransmitter glutamate, and maintaing acid-based balance in the kidney. There are alternate splicing results in multiple transcript variants in the Glutaminase gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686O15119; FLJ10358; GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase
基因别名: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; RATGLUT
UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264
Entrez Gene ID: (Human) 2744, (Mouse) 14660, (Rat) 24398
