Invitrogen

K-Ras Polyclonal Antibody

Name: K-Ras Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-44339
Availability: InStock

高级验证

1个已发表图片

7 篇参考文献

查看其他24个K-Ras抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

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K-Ras Antibody (PA5-44339) in WB

Western blot analysis of human RPMI-8226 cells using an anti-K-Ras polyclonal antibody (Product # PA5-44339).

K-Ras Antibody in Immunohistochemistry (IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

K-Ras Antibody — Degradation of K-RAS requires autophagosome formation. (a). PANC-1 pancreatic and HCT116 colorectal tumor cells were transfected with plasmids to express K-RAS V12-GFP and K-RAS V12-RFP. In parallel, cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down the expression of ATG5, Beclin1 or LAMP2. Twenty-four h after transfection cells were treated with vehicle control or with neratinib (100 nM). Cells were imaged at 60X magnification over the time course shown in the Figure (representative images from three studies are shown). Analyses for densitometric scanning of fluorescence intensity at 3h were performed at 10X magnification on > 120 cells per condition (n = 3 SD) * p < 0.05 less than vehicle control exposure. (b). PANC-1 and HCT116 cells were transfected with a scrambled control or with siRNA molecules to knock down the expression of K-RAS or N-RAS. Twenty-four h after transfection, cells were fixed in place and immune-staining performed to detect the fluorescence intensity under each condition. Analyses for densitometric scanning of fluorescence intensity at 3h were performed at 10X magnification on > 120 cells per condition (n = 3 SD) * p < 0.05 less than vehicle control exposure. (c). Uveal melanoma cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down the expression of ATG5 or Beclin1. Twenty-four h after transfection cells were treated with vehicle control or with neratinib (100 nM). Cells {KD}

Immunogen image

Immunogen Image K-Ras Polyclonal Antibody

产品信息

PA5-44339

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the N-terminal of human KRAS (aa 2-51).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

50 µg

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2609818

产品详细信息

Peptide sequence: TEYKLVVVGA GGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%

靶标信息

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。