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Invitrogen
Mu-Calpain Monoclonal Antibody (2H2A7C2)
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Mu-Calpain Antibody (MA3-941) in ICC/IF
Immunofluorescence analysis of Mu-Calpain was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Mu-Calpain (2H2A7C2) Monoclonal Antibody (Product # MA3-941) at 1:20 dilution in 0.1% BSA, incubated at 4 degr... View More
产品信息
MA3-941
产品规格
种属反应
Bovine,
Human,
Pig,
Rat
已发表种属
Bovine
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2H2A7C2
抗原
Purified bovine skeletal muscle 80 kDa mu-calpain subunit.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-941 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, and pig cultured cells. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
MA3-941 has been successfully used in Western blot, IF and immunocytochemistry procedures. By Western blot, this antibody detects an ~80 kDa protein representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in porcine LLC-PK1 cells with MA3-941 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-941 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 245-265 (domain II) of human mu-calpain.
靶标信息
The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: calcium activated neutral protease; calcium-activated neutral protease 1; Calcium-activated neutral proteinase 1; calpain 1 large subunit; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP 1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; mucalpain; muCANP
基因别名: CANP; CANP 1; CANP1; CANPL1; CAPN1; Cls1; muCANP; muCL; PIG30; SPG76
UniProt ID: (Pig) P35750, (Bovine) Q27970, (Human) P07384, (Rat) P97571
Entrez Gene ID: (Pig) 397027, (Bovine) 281661, (Human) 823, (Rat) 29153
