Invitrogen

PDI Recombinant Rabbit Monoclonal Antibody (016), FITC

Name: PDI Recombinant Rabbit Monoclonal Antibody (016), FITC
Brand: Invitrogen
SKU: MA5-40969

查看其他30个PDI抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

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PDI Antibody (MA5-40969) in Flow

Flow cytometry analysis of Human PDI (PDI) expression in HeLa cells. The cells were treated according to manufacturer’s manual, and stained with PDI Recombinant Rabbit Monoclonal Antibody (016), FITC (Product # MA5-40969). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

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产品信息

MA5-40969

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

016

抗原

Recombinant Human P4HB protein

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein A

保存液

PBS with 0.5% BSA

内含物

0.03% ProClin 300

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2898730

产品详细信息

May be stored for twelve months without detectable loss of activity. Protect from prolonged exposure to light and do not freeze. Sodium azide is toxic to cells and should be disposed of properly so flush with large volumes of water during disposal.

靶标信息

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Eps1p; glutathione-insulin transhydrogenase; p55; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55

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基因别名: CLCRP1; DSI; ERBA2L; GIT; P4HB; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB

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UniProt ID: (Human) P07237

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Entrez Gene ID: (Human) 5034

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功能

protein disulfide isomerase activity procollagen-proline 4-dioxygenase activity integrin binding protein binding enzyme binding poly(A) RNA binding protein heterodimerization activity chaperone

参与通路

response to reactive oxygen species protein folding peptidyl-proline hydroxylation to 4-hydroxy-L-proline response to endoplasmic reticulum stress lipoprotein biosynthetic process cell redox homeostasis positive regulation of viral entry into host cell cellular response to hypoxia regulation of oxidative stress-induced intrinsic apoptotic signaling pathway