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Invitrogen
PMCA4 ATPase Monoclonal Antibody (JA9)
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FIGURE: 1 / 14
PMCA4 ATPase Antibody (MA1-914) in ICC/IF
Immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) using Product # MA1-914 on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) using Product # MA1-934 on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.
Product Details
MA1-914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Pig,
Rat
Published species
Amphibian,
Bovine,
C. elegans,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
JA9
Immunogen
Purified human erythrocyte PMCA.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine and rat tissues.
MA1-914 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.
The MA1-914 antigen is purified human erythrocyte PMCA ATPase. The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1; ATPase, Ca++ transporting, plasma membrane 4 tv2; Matrix-remodeling-associated protein 1; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; plasma membrane calcium ATPase 4; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 4; plasma membrane calcium-pumping ATP-ase; Plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; sarcolemmal calcium pump
Gene Aliases: ATP2B2; ATP2B4; MXRA1; PMCA4; PMCA4b; PMCA4x
UniProt ID: (Bovine) D3K0R6, (Human) P23634, (Rat) Q64542
Entrez Gene ID: (Bovine) 282146, (Human) 493, (Pig) 100623420, (Rat) 29600
calmodulin binding
ATP binding
protein phosphatase 2B binding
nitric-oxide synthase inhibitor activity
metal ion binding
calcium-dependent protein binding
nitric-oxide synthase binding
calcium-transporting ATPase activity
protein binding
sodium channel regulator activity
PDZ domain binding
scaffold protein binding
primary active transporter
regulation of transcription from RNA polymerase II promoter
cellular calcium ion homeostasis
negative regulation of gene expression
negative regulation of nitric oxide mediated signal transduction
urinary bladder smooth muscle contraction
negative regulation of angiogenesis
sperm motility
positive regulation of peptidyl-serine phosphorylation
negative regulation of blood vessel endothelial cell migration
negative regulation of nitric oxide biosynthetic process
negative regulation of nitric-oxide synthase activity
response to hydrostatic pressure
calcium ion transmembrane transport
negative regulation of calcineurin-NFAT signaling cascade
cellular response to epinephrine stimulus
negative regulation of the force of heart contraction
negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process
negative regulation of arginine catabolic process
calcium ion export
negative regulation of cellular response to vascular endothelial growth factor stimulus
regulation of cell cycle G1/S phase transition
positive regulation of protein localization to plasma membrane
negative regulation of cardiac muscle hypertrophy in response to stress
negative regulation of citrulline biosynthetic process
cellular response to acetylcholine
positive regulation of cAMP-dependent protein kinase activity
neural retina development
spermatogenesis
hippocampus development
ion transmembrane transport
calcium ion transmembrane import into cytosol
calcium ion import across plasma membrane
negative regulation of adrenergic receptor signaling pathway involved in heart process
negative regulation of peptidyl-cysteine S-nitrosylation
regulation of sodium ion transmembrane transport
regulation of cardiac conduction
calcium ion transport
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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