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Invitrogen
PROP1 Polyclonal Antibody
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FIGURE: 1 / 2
PROP1 Antibody (PA5-100890) in ICC/IF
Immunocytochemistry analysis of PROP1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PROP1 polyclonal antibody (Product # PA5-100890) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100890
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PROP1(Accession O75360), corresponding to amino acid residues A98-P148.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PROP1.
Target Information
PROP1 encodes a paired-like homeodomain transcription factor in the developing pituitary gland. Expression occurs prior to and is required for expression of pou domain transcription factor 1, which is responsible for pituitary development and hormone expression. Mutations in this gene have been associated with combined pituitary hormone deficiency-2 as well as deficiencies in luteinizing hormone, follicle-stimulating hormone, growth hormone, prolactin, and thyroid-stimulating hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ames dwarf; paired like homeodomain factor 1; pituitary specific homeodomain factor; pituitary-specific homeodomain factor; prophet of Pit-1; prophet of Pit1, paired-like homeodomain transcription factor
Gene Aliases: CPHD2; df; PROP-1
UniProt ID: (Human) O75360, (Mouse) P97458
Entrez Gene ID: (Human) 5626, (Mouse) 19127, (Rat) 266738
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
protein binding
beta-catenin binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
organ morphogenesis
dorsal/ventral pattern formation
cell migration
hypothalamus cell differentiation
pituitary gland development
adenohypophysis development
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
gland development
hypophysis morphogenesis
somatotropin secreting cell differentiation
central nervous system development
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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