Invitrogen

Phospho-Paxillin (Tyr118) Polyclonal Antibody

Name: Phospho-Paxillin (Tyr118) Polyclonal Antibody
Brand: Invitrogen
SKU: 44-722G

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45个已发表图片

102 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Phospho-Paxillin (Tyr118) Antibody in Western Blot (WB)

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Phospho-Paxillin (Tyr118) Antibody (44-722G) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of COS-7 (Lane 1), COS-7 treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3), A431 treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) and HeLa treated with 100 uM Pervanadate for 1 hour (Lane 6).The blots were probed with Anti-Phospho-Paxillin pTyr118 Rabbit Polyclonal Antibody (Product # 44-722G, 1:500 ... View More

Phospho-Paxillin (Tyr118) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Paxillin (Tyr118) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Paxillin (Tyr118) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-Paxillin (Tyr118) Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

Phospho-Paxillin (Tyr118) Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image Phospho-Paxillin (Tyr118) Polyclonal Antibody

产品信息

44-722G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看48篇已发表文章 48篇已发表文章

免疫组化 (IHC)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

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Proximity Ligation Assay (PLA) (PLA)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Chicken, Dog, Human, Mouse, Pig, Rat, Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533733

产品详细信息

The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 118.

Positive controls used with this antibody in western blotting were normal mouse mammary gland (NMuMG) cells, NMuMG transfected with EGFP-tagged paxillin +/- TGFbeta, and human epidermoid carcinoma cells (A431) +/- EGF.

靶标信息

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。