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Invitrogen
Zap-70 Monoclonal Antibody (1E7.2), PE
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Zap-70 Antibody (MHZAP7004) in Flow
Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MHZAP7004
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Rhesus monkey
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1E7.2
抗原
GST-fusion protein of Human ZAP70, amino acids 282-307
偶联物
PE
PE
PE
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
纯化类型
purified
保存液
PBS with sucrose, 4mg/mL BSA
内含物
0.1% sodium azide
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
靶标信息
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Analyte Specific Reagent
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生物信息学
蛋白别名: 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa
基因别名: mrtle; mur; Srk; ZAP-70; Zap70
UniProt ID: (Mouse) P43404
Entrez Gene ID: (Mouse) 22637
nucleotide binding
phosphotyrosine binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
immune system process
protein phosphorylation
immune response
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
beta selection
positive thymic T cell selection
negative thymic T cell selection
thymic T cell selection
innate immune response
positive regulation of T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
protein autophosphorylation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
